Seyed Hossein Mirdehghan; Zahra Aghamolaei
Abstract
Two experiments were designed to evaluate the addition of essential oils to preservative solution and subsequent effects on postharvest quality and vase life of Gladiolus ‘Sorati’ cut flowers. In first experiment, the solutions of savory (Satureja hortensis.), ajowan (Trachyspermum ammi) ...
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Two experiments were designed to evaluate the addition of essential oils to preservative solution and subsequent effects on postharvest quality and vase life of Gladiolus ‘Sorati’ cut flowers. In first experiment, the solutions of savory (Satureja hortensis.), ajowan (Trachyspermum ammi) and thyme (Thymus vulgaris) were applied at the concentrations of 2, 4, and 6 (mg L−1), distilled water + 2% sucrose were considered as control treatment. In second experiment, the cut flowers pulsed with CaCl2 and AgNO3 for 1 h and then transferred to preservative solution contain 2 mg L-1 of savory, ajowan, thyme and distilled water (control). All cut flowers were kept at 25±2oC for 20 days and qualitative properties were evaluated during postharvest periods. Savory 2 mg L−1 exhibited as the most effective treatment for minimizing dehydration of the gladiolus cut flowers by enhancing the water absorption and flower weight rate than other treatments. The interaction of pulsing with CaCl2 and essential oils could maintain the quality of cut flowers.
Raziyeh Robatjazi; Parto Roshandel; Sa’dollah Hooshmand
Abstract
In the present study, improvement of salt tolerance in basil (as a salt-sensitive plant) was investigated through silicon (Si) nutrition. Basil plants were subjected to silicon (0, 3 mM) and salinity (0, 50, 100, 150 and 200 mM NaCl) for a duration of one month. Salt stress significantly decreased the ...
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In the present study, improvement of salt tolerance in basil (as a salt-sensitive plant) was investigated through silicon (Si) nutrition. Basil plants were subjected to silicon (0, 3 mM) and salinity (0, 50, 100, 150 and 200 mM NaCl) for a duration of one month. Salt stress significantly decreased the biomass of basil. Si supplement (3 mM) resulted in a considerable increase (averagely +135%) in the biomass of salinized plants. Salt stress significantly decreased photosynthetic pigments concentrations, but Si supplement improved total chlorophyll concentration (averagely up to +217% compared to salinized plants). This improvement in pigment concentrations also occurred for carotenoids content (+123%). Salinity increased lipid peroxidation and H2O2 level in the aerial parts of the basil plants, but Si decreased lipid peroxidation (-49.1%) and H2O2 content (-29%) under salinity condition. Results showed salinity (alone) or together with Si, increased the level of polyphenols and also the level of radical scavenging activities in the aerial parts of basil but this effect was much more in plants co-treated with Si and salinity. Si nutrition increased the activity of SOD, APX and GPX in response to salt stress, but it did not affect CAT activity. Overall, Si supplement could induce salt tolerance in basil plants by improving photosynthesis, membrane integrity, and detoxification of toxic radicals. Furthermore, silicon increased the medicinal properties of basil via elevating its antioxidant capacity under salt stress.
Maryam Chavoushi; Khosrow Manoochehri Kalantari; Mohamad Javad Arvin
Abstract
Salinity stress is one of the main limiting factors for optimum agricultural productivity of safflower, Carthamus tinctorius L., in arid and semi-arid regions. It could leads to significant changes in plant biochemical, physiological, and growth traits. Salinity induced endogenous rise in jasmonic acid ...
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Salinity stress is one of the main limiting factors for optimum agricultural productivity of safflower, Carthamus tinctorius L., in arid and semi-arid regions. It could leads to significant changes in plant biochemical, physiological, and growth traits. Salinity induced endogenous rise in jasmonic acid and its methyl esters (MeJA) has been reported. In the present study, effects of salinity stress (6 and 12 ds m-1) and the exogenous application of MeJA (0.1 and 0.5 mM) on the leaf number, shoot fresh weight, shoot length, chlorophyll a/b, soluble sugar, proline, and malondialdehyde (MDA) contents were investigated in two safflower varieties (Isfahan and IL111). Salinity stress negatively affected the growth of both varieties. Lipid peroxidation was not observed in Isfahan variety, but it significantly increased in the salinity resistant safflower, IL111. Soluble sugar and proline as the important osmoprotectants and free radical scavengers were elevated by salinity stress. Exogenous application of MeJA to the salinity stress-imposed plants slightly improved the growth due to inductions in the rate of photosynthesis; however, MeJA application impaired the growth of non-stressed plants because of induction of stomatal closure and as a result reduced photosynthesis.
Farid Moradinezhad; Mehdi Khayyat
Abstract
We examined the effectiveness of intermittent warming (IW), hot water (HW), salicylic acid (SA), and calcium chloride (CaCl2) on the postharvest life of pomegranate fruit cv. ‘Shishe-Kab’ and extending fruit shelf life during cold storage (3°C). Fruit were subjected to cycles of 1 d at ...
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We examined the effectiveness of intermittent warming (IW), hot water (HW), salicylic acid (SA), and calcium chloride (CaCl2) on the postharvest life of pomegranate fruit cv. ‘Shishe-Kab’ and extending fruit shelf life during cold storage (3°C). Fruit were subjected to cycles of 1 d at 17 ± 1°C every 6 d of storage at 3°C under IW conditions. Pre-storage treatments were HW (50°C) for 3 min, SA (2mmol L-1), or a combination of SA (2 mmol L-1) and CaCl2 (2%) solutions for 3 min at 20°C. Weight loss was not affected by HW or chemical treatments, but firmness decreased significantly in HW-treated fruit when they were subjected to IW. The lowest quantity of unmarketable fruit was observed in fruit treated with a combination of SA and CaCl2, regardless of whether stored in cold storage (CS) or exposed to IW. Regardless of HW and chemical treatments, IW significantly albeit slightly increased shelf life and reduced fruit decay compared with the control. However, the longest fruit shelf life (19 wk) was obtained with a combination of HW, SA, and CaCl2 compared with the control (11 wk), especially under long-term cold storage.
Mehrnaz Zarei; Hassan Salehi; Abolfazl Jowkar
Abstract
Plant propagation originated from the mature tissues is the most desirable method in producing clonal plants, however microbial contamination usually is the main concern during regeneration processes. Moreover, commercial production of plants using clonal propagation is considered as high throughput ...
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Plant propagation originated from the mature tissues is the most desirable method in producing clonal plants, however microbial contamination usually is the main concern during regeneration processes. Moreover, commercial production of plants using clonal propagation is considered as high throughput method due to the removal of seasonal barriers, lower cost of production with higher yield when compared to the conventional propagation methods. In this research the regulatory roles of 2-iso-pentenyl adenine (2iP) and Zeatin (Z) along with two temperature regimes, 22 and 28 °C, on mature explants of Picea abies (L.) H. Karst was evaluated for adventitious shoot regeneration. Norway spruce micro-cuttings considerably benefited from Ridomil and Benomyl treatments for fungal decontamination in explants taken in spring or fall. The highest percentage of regenerated explants (68.75%) as well as the highest numbers of adventitious shoots per explant were obtained in MS media supplemented with 4 µM Z. Furthermore, the highest length of proliferated shoot (1.17 cm) was achieved at 3.2 µM 2iP treatment. However, there were no significant differences in the length of adventitious shoots using different concentrations of 2iP. Incubating explants at 28˚C resulted in null or deformed regeneration in Norway spruce.
Tahereh Goudarzi; Leila Tabrizi; Hossain Ali Alikhani; Vahideh Nazeri; Farzad Najafi
Abstract
To evaluate the effects of four licorice plant growth-promoting bacteria (PGPB), i.e. Pantoea agglomerans (S72), Serratia rubidaea (S28) Pseudomonas azotoformans (E101), Pseudomonas frederiksbergensis (E56) on licorice seed germination and seedling growth, two experiments were conducted at the Research ...
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To evaluate the effects of four licorice plant growth-promoting bacteria (PGPB), i.e. Pantoea agglomerans (S72), Serratia rubidaea (S28) Pseudomonas azotoformans (E101), Pseudomonas frederiksbergensis (E56) on licorice seed germination and seedling growth, two experiments were conducted at the Research Laboratory and Greenhouse, University of Tehran in 2019. Treatments were bacterial strains in eight levels (sole or in combination) and growing media at four levels of M1 (sand + perlite (1: 1)), M2 (soil + cow manure), M3 (soil + mycorrhiza fungi) and M4 (soil + mycorrhiza fungi+ cow manure). Germination quality criteria and some morphological traits of two-month-old seedlings were evaluated. The results of seed germination showed that in most of the evaluated traits, bacterial treatments performed better than the control, although in some cases, there were no significant differences with the control. Also, in some cases, endophytic bacteria had an effective role in improving seed germination index compared to rhizospheric bacteria. According to the experiment results of seedling growth, M4 in combination with two endophytic bacteria (E101 and E56), of the Pseudomonas genus in licorice plants, had a superior performance in improving the initial growth and establishment qualities of the licorice plants. Regarding most of the traits, the co-application of mycorrhiza with S28 (M3B3) showed the lowest values. The results of this study indicated the potential use of licorice endophytic bacteria as a source of biofertilizer for the improvement of licorice seed germination and growth and, if possible, for similar applications in other plant species. AbbreviationsM1 (sand + perlite (1: 1)), M2 (soil + cow manure), M3 (soil + mycorrhiza fungi) and M4 (soil + mycorrhiza fungi + cow manure), Plant growth-promoting bacteria (PGPB), Indole-3-acetic acid (IAA), B1 (Pantoea agglomeransz (S72)), B2 (Serratia rubidaea (S28)), B3 (Pseudomonas azotoformans (E101)), B4 (Pseudomonas frederiksbergensis (E56)
Rohollah Abdoli Nejad; Akhtar Shekafandeh
Abstract
A major portion of the Iranian fig (Ficus carica) industry is located in high-salinity regions, and salinity is an important limiting factor in the production of this fruit. The present study was conducted to investigate the changes of leaf antioxidant-enzyme activity, proline and total protein content ...
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A major portion of the Iranian fig (Ficus carica) industry is located in high-salinity regions, and salinity is an important limiting factor in the production of this fruit. The present study was conducted to investigate the changes of leaf antioxidant-enzyme activity, proline and total protein content in two fig cultivars with two leaf shapes: ‘Anjir Sabz’ with lobate and palmate leaves, and ‘Shah Anjir’ with lobate and palmate leaves, under salt stress condition. The seedlings were exposed to different NaCl concentrations so that six different electrical conductivity levels of 0.6, 3, 6, 9, 12 and 15 dSm-1 were achieved in pots. This experiment was performed as factorial based 6×2(2) in a completely randomized design with four replications and two seedlings in each replicate. The results showed that as the soil salinity increased, the proline and protein contents of both cultivars were increased. However, palmate leaves of both cultivars accumulated more proline and protein than those of their lobate leaves. The activities of antioxidant enzymes increased in seedlings of both cultivars; however, superoxide desmutase and catalase showed more activity in palmate leaves than lobate leaves. There were no significant differences between the two leaf shapes in relation to peroxidase. The results seem to suggest that seedlings with palmate leaves are more salt tolerant than seedlings with lobate leaves.
MD. Farid Hossain
Abstract
This research aimed to evaluate the effects of different potting media and pot sizes on yield and quality attributes (TSS%, Vitamin C, pH) of cherry tomato in the winter season under field conditions. Cherry tomato was cultivated in four different size pots by using four types of potting media. Plastic ...
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This research aimed to evaluate the effects of different potting media and pot sizes on yield and quality attributes (TSS%, Vitamin C, pH) of cherry tomato in the winter season under field conditions. Cherry tomato was cultivated in four different size pots by using four types of potting media. Plastic pots ranged in capacity (2L, 6L, 10L and 14L). Each treated pot was filled with air dried field loamy soil without fertilizer (control) and organic fertilizers, namely, cow dung (1:1), poultry litter (1:1) and vermicompost (1:1) which were used as treatments. In total, 16 treatment combinations were arranged in a randomized complete block design. The highest total number of fruits and fruit yield per plant of cherry tomato was obtained from the plants raised in pots with cow dung mixture soil (1:1). Organic fertilizers in pot soil significantly affected the TSS% and pH value of ripened fruits. The pot size affected the total fruit yield per plant significantly, and the best yield was 2.2 times higher than that of plants in the smallest pots. The highest total number of fruits per plant (108.75), fruit yield per plant (504.25g) and yield per hectare (20.17t) was obtained from plants in 14L size pots by using cow dung mixture soil (1:1). It was concluded that the 14L pot and cow dung mixture soil (1:1) were the best treatments for the cultivation of cherry tomato in homestead gardening.
Majid Rahemi; Fateme Yazdani; Sahar Sedaghat
Abstract
Selection of frost tolerant cultivars and understanding the mechanisms of frost hardiness could help to improve freezing resistance in olive plants. Olive cultivars may differ in frost hardiness due to differential survival of specific organs. The aim of this study was to screen different olive cultivars ...
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Selection of frost tolerant cultivars and understanding the mechanisms of frost hardiness could help to improve freezing resistance in olive plants. Olive cultivars may differ in frost hardiness due to differential survival of specific organs. The aim of this study was to screen different olive cultivars based on their stomatal density and metabolic modifications under cold conditions. The ‘Zard’ cultivar had the lowest while ‘Derak’ had the highest stomatal density, respectively. In another experiment, where entire potted olive plants were subjected to freezing stress (0, -6, -12 and -18 ˚C), ‘Zard’ and ‘Dehghan’ were found to be the most tolerant cultivars. They showed the lowest starch content, ionic leakage and wood injury. They also had the highest reducing sugar, phenolic and proline contents among studied cultivars. We concluded that ‘Zard’ and ‘Dehghan’ are the most tolerant cultivars and ‘Derak’, ‘Dakal’ and ‘Shiraz’ are the most sensitive cultivars to freezing injury.
Mohammad Hosein Shamshiri; Mohammad Reza Hasani
Abstract
This study was conducted to determine the combined effects of salicylic acid (SA) and arbuscular mycorrhizal fungi (AMF) (Glomus mosseae) symbiosis on the growth of pistachio seedlings (Pistacia vera ‘Abareqi’) grown in the greenhouse under different drought stress (DS) levels. The arbuscular ...
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This study was conducted to determine the combined effects of salicylic acid (SA) and arbuscular mycorrhizal fungi (AMF) (Glomus mosseae) symbiosis on the growth of pistachio seedlings (Pistacia vera ‘Abareqi’) grown in the greenhouse under different drought stress (DS) levels. The arbuscular mycorrhizal fungi (AMF) colonization or exogenous SA treatment could increase ‘Abareqi’ pistachio seedlings tolerance to DS. Application of SA on AMF-inoculated seedlings further promoted drought tolerance, as indicated by an alleviated plant biomass and water relations compared to the respective treatments. The analysis of proline and soluble carbohydrates showed that the increased drought tolerance in the treated plants may be associated, at least in part, with increasing of proline accumulation in the leaves of stressed plants.
Rouzbeh Abbaszadeh; Kosar Alimohammad; Romina Zarrabi Ekbatani
Abstract
Fig (Ficus carica L.) is a perishable fruit and it needs to use new techniques in order to increase shelf life of this product. In present study, atmospheric cold plasma as a non-thermal treatment was utilized for preserving fresh fig quality. Dielectric barrier discharge plasma was applied to fruits ...
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Fig (Ficus carica L.) is a perishable fruit and it needs to use new techniques in order to increase shelf life of this product. In present study, atmospheric cold plasma as a non-thermal treatment was utilized for preserving fresh fig quality. Dielectric barrier discharge plasma was applied to fruits in two steps. Durations of first treatments were 1 and 5 minutes. Based on this step results, main experiments were conducted for 30, 90 and 180s and cold plasma was applied to packed and unpacked fig samples. Quality was sensory evaluated in terms of color, texture, odor, appearance and overall acceptance. Firmness, color indices (L*, a* and b*),, total soluble solids and pH were also determined. Subjective measurements showed significant shelf life improvement of treated figs compared to control samples while objective quality attributes were not changed except pH and a* which are not undesired. Direct application of plasma for 90 s and in-package treatment for 30 s were suggested for further investigation and considering the practical aspects, pulsed plasma processing of packed figs for 30 s is recommended. It is concluded that atmospheric cold plasma has a potential for prolonging the shelf life of fresh fig fruits.
Mohsen Azadbakht; Mohammad Javad Mahmoodi; Mohammad Vahedi Torshizi
Abstract
Nowadays, due to the necessity of increasing quality awareness in the food sector and its health, the non-destructive computed tomography (CT) method, which is one of the most widely used methods because of the ability to detect internal bruise in a non-destructive way, attracted so much attention. By ...
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Nowadays, due to the necessity of increasing quality awareness in the food sector and its health, the non-destructive computed tomography (CT) method, which is one of the most widely used methods because of the ability to detect internal bruise in a non-destructive way, attracted so much attention. By using the non-destructive CT method a total of 81 healthy pears was selected and then subjected to quasi-static and dynamical loading. The experiment was performed on wide edge quasi-static pressure of 70, 100, 130 N and thin edge of 15, 20, 25 N and dynamic load of 300, 350, 400 g and storage period for 5, 10 and 15 days, to investigate the different effects of loading forces and storage periods on the percentage of the bruise and its relation with the qualitative properties such as phenol, antioxidant and vitamin C contents and firmness. The results of the experiments showed that the highest and lowest percentages of the bruise were related to a load of 400 N of 15 days and a 15 N 5-day thin line with values of 47.36 and 0.007, respectively. The highest and lowest physiological values were 15 N load of the 5-day thin edge and the 400 N of 15-day impact. Finally, the highest antioxidant content was 51.5% for 300 g dynamic loading force and 5- day storage, 28.86 mg/100g phenol for loading force of 70 N wide edge and 5 day storage and 7.4 mg/100ml vitamin C for loading force of 70 N wide edge and 5 day storage. Finally, according to the obtained results, there was an inverse relationship between the amount of bruising and chemical properties of pear.
Azam Nikbakht Dehkordi; Nadali Babaeian; Sajede Karimpour; Pedro Martinez-Gomez; Manuel Rubio; Nadali Bagheri
Abstract
Plum pox or sharka, a viral disease caused by Plum pox virus (PPV), severely affects the production of Prunus species in Europe. The first evidence of sharka was reported in Iran in 2000 . Due to the economic impact of this disease on crop production, recent advances in the term of biology, epidemiology, ...
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Plum pox or sharka, a viral disease caused by Plum pox virus (PPV), severely affects the production of Prunus species in Europe. The first evidence of sharka was reported in Iran in 2000 . Due to the economic impact of this disease on crop production, recent advances in the term of biology, epidemiology, and disease management are provided in this paper to assure awareness among growers and professionals involved in Prunus production. This study will provide fundamental knowledge about this virus to guaranty the successful detection and controlling of sharka disease in Iran.
Peter Abraham; Olalekan Oyeleke Banwo; Boniface David Kashina; Mathew Dada Alegbejo
Abstract
This study is aimed to detect weed hosts of Tomato Yellow Leaf Curl Virus (TYLCV) in field-grown tomato (Solanum lycopersicum L.) in Sudan savanna regions (Gombe, Jigawa and Kano states) of Nigeria during 2017 and 2018 dry and wet seasons. Three farms each from three Local Government Areas (LGAs) of ...
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This study is aimed to detect weed hosts of Tomato Yellow Leaf Curl Virus (TYLCV) in field-grown tomato (Solanum lycopersicum L.) in Sudan savanna regions (Gombe, Jigawa and Kano states) of Nigeria during 2017 and 2018 dry and wet seasons. Three farms each from three Local Government Areas (LGAs) of each state were surveyed. Ten symptomatic and asymptomatic weed samples within and around each farm (n=1080) were randomly collected and assayed against TYLCV using Triple Antibody Sandwich Enzyme-linked Immunosorbent Assay (TAS-ELISA). Based on the obtained results, 14 weed species from 12 families were detected as alternative host of TYLCV in all states surveyed but with variation in distribution. Gombe and Kano states had significantly (p ≤ 0.05) the highest number (7) of TYLCV weed hosts while the lowest number (4) of TYLCV weed hosts was recorded in Jigawa state. Cassia obtusifolia Linn., significantly (p ≤ 0.05) showed the highest TYLCV frequency rating of 12.1%. The study reports for the first time 13 weed species naturally occurring as an alternative host of TYLCV in Nigeria. The detection of these weed hosts of TYLCV will give a better understanding of the virus disease epidemiology for its effective management. Exploring more weed hosts of TYLCV and molecular characterization of the virus in these weeds for the possible evolution of novel strain(s) in the region is recommended.
Mansoureh Danesh; Mahmoud Lotfi; Shiva Azizinia
Abstract
Iran is a part of the secondary centre of origin of melons, and therefore, there is a wealth of genetic variation of these species in this country. The Amplified Fragment Length Polymorphism (AFLP) marker was applied to investigate the genetic variations among five major Iranian melon cultigens (Cucumis ...
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Iran is a part of the secondary centre of origin of melons, and therefore, there is a wealth of genetic variation of these species in this country. The Amplified Fragment Length Polymorphism (AFLP) marker was applied to investigate the genetic variations among five major Iranian melon cultigens (Cucumis melo) and ‘Ananasi’ as a general well-known cultivar. Ten primer pairs were used on 90 individuals producing 318 polymorphic fragments, with an average of 31.8 fragments per primer combination. The polymorphism rates ranged from 80 to 100%. The genetic similarities among accessions were calculated according to Dice’s Similarity Index and used to construct a dendrogram based on the Unweighted Pair Group Method with Arithmetic Average (UPGMA). The genetic distance estimates based on AFLPs ranged from 0.29 to 0.63, with a mean of 47±0.3. Iranian melon genotypes and the ‘Ananasi’ cultivar were considered as two separate groups on the cluster analysis. The principal coordinate analysis showed a separate allocation of the melon cultivar groups. The results demonstrated a wide diversity of Iranian melon cultigens. The high number of alleles and the high expected genetic diversity detected with the AFLP marker indicated that the Iranian melon cultigens had distinctive characteristics and were an important genetic diversity pool, which made them a valuable source of breeding materials.
Farid Moradinezhad; Fatemeh Setayesh; Sohrab Mahmoodi; Mehdi Khayyat
Abstract
Jujube (Ziziphus jujuba Mill.) fruit is an important medicinal plant in Iran. The harvesting time is a crucial for jujube fruit quality. Several characteristics of jujube fruit when harvested at four development stages; white mature (light green), crisp mature (white-red), fully mature (red) and fully ...
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Jujube (Ziziphus jujuba Mill.) fruit is an important medicinal plant in Iran. The harvesting time is a crucial for jujube fruit quality. Several characteristics of jujube fruit when harvested at four development stages; white mature (light green), crisp mature (white-red), fully mature (red) and fully ripe (dehydrated brown) were evaluated. Fruit weight, diameter, volume, pulp to stone ratio, firmness, titratable acidity (TA), total soluble solids (TSS), ascorbic acid, total phenolics content, protein, ash and chlorophyll fluorescence parameters were determined. The results showed that fruit dry weight, TSS, TSS/TA were increased significantly, while firmness, protein content and ash weight were decreased as jujube fruit harvested at progressed development stages. Ascorbic acid in fruit was decreased from white to crisp mature stage and thereafter increased significantly to fully mature stage. However, the content of total phenol increased significantly from white to crisp mature stage and thereafter decreased at more maturity and ripening stages. Moreover, chlorophyll fluorescence parameters were influenced by harvesting time. The F0 and Fm showed a positive significant correlation with total phenolic, ascorbic acid and protein content and a negative significant correlation with DW. Therefore, chlorophyll fluorescence maybe a helpful, nondestructive technique to evaluate the nutritional quality changes in jujube fruit. Overall, jujube fruit harvested at crisp (white-red) and fully mature (red) stages, seem best in respect of postharvest handling and nutritional criteria.
Saber Avestan; Lotfali Naseri; Roghayeh Najafzadeh
Abstract
Nano-fertilizers can increase value of products in agriculture. Iron plays many important and essential roles in plant growth and development as compared to other micronutrients. In the present study, effects of different levels of enriched nano chelated Iron fertilizer (25, 50, 100, and 200 mg l-1) ...
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Nano-fertilizers can increase value of products in agriculture. Iron plays many important and essential roles in plant growth and development as compared to other micronutrients. In the present study, effects of different levels of enriched nano chelated Iron fertilizer (25, 50, 100, and 200 mg l-1) were investigated in comparison with the common Iron (FeSO4.7H2O) on in vitro proliferation of apple explants cultivar 'Gala' in the MS medium. The results showed that enrichednano chelated Iron increased growth and proliferation of apple in terms of number of nodes, shoots, and leaves, nodes and shoots length, leaf area, fresh and dry weights of shoot and chlorophyll index. The maximum growth and proliferation was observed at 100 mg l-1 of enrichednano chelated Iron, while in higher concentration (200 mg l-1), the growth was decreased due to the Iron toxicity. According to the obtained results, 100 mg l-1 of enrichednano chelated Iron can be added for increasing growth on in vitro proliferation of 'Gala' apple cultivar. The present study is the first report of the effects of enrichednano chelated Iron fertilizer on growth and in vitro proliferation of apple that can be useful for in vitro culture of the plant.of enriched nano iron chelated can be added for increasing growth on in vitro proliferation of 'Gala' apple cultivar. The present study is the first report of the effects of enriched nano chelated iron fertilizer on growth and in vitro proliferation in apple that can be useful for in vitro culture of the plant.
Maryam Eslami; Fatemeh Nasibi; Khosrow Manouchehri Kalantari; Masoud Khezri; Hakimeh Oloumi
Abstract
Pistachio yield are often negatively affected by some physiological problems such as abscission of inflorescence buds and fruits, deformed or blank nuts, and non-split shells. In the present study the effect of exogenous application of arginine (Arg) (a substrate for nitric oxide (NO) synthase) and sodium ...
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Pistachio yield are often negatively affected by some physiological problems such as abscission of inflorescence buds and fruits, deformed or blank nuts, and non-split shells. In the present study the effect of exogenous application of arginine (Arg) (a substrate for nitric oxide (NO) synthase) and sodium nitroproside (SNP), as a NO-donor was investigated on yield production, fruit and inflorescence buds abscission, and physiological parameters of Pistacia vera. The experiment was conducted in randomized complete block design with factorial structure in a commercial pistachio orchard. Factors were included two levels of Arg (0.5 and 1 mM), two levels of SNP (50 and 100 μM) and their combination applied at two distinct time; one week before full bloom (first stage) and five weeks after full bloom (second stage). Comparing to SNP-treated trees, Arg was more effective on growth and physiological parameters of shoots and on fruit and inflorescence buds abscission, and on fruit parameters such as split and non-split shells, blank nuts, number of nuts per ounce. Effects of these treatments were considerably depending on the time of application and the concentrations of applied compounds. It is proposed that the effects of Arg and SNP may be related to the NO signaling and polyamines production.
Masoud Sharifkhah; Davood Bakhshi; Mohammadreza Pourghayoumi; Soma Abdi; Hossein Hokmabadi
Abstract
To obtain the best quality and quantity of pistachio fruits, knowing the most suitable time of pollination, is of paramount importance. This study was conducted to investigate the effect of pollination time, different stages of female flower development, including A (lower florets opened and clusters ...
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To obtain the best quality and quantity of pistachio fruits, knowing the most suitable time of pollination, is of paramount importance. This study was conducted to investigate the effect of pollination time, different stages of female flower development, including A (lower florets opened and clusters were green), B (lower and medial florets opened and clusters were green), C (all florets opened and clusters were light green), D (the stigma in the lowest florets was light pink), and E (the stigma in the lowest and medial florets was pink) on quantitative and qualitative characteristics of fruits in three pistachio cultivars (‘Ghermez Peste’, ‘Kal Khandan’, and ‘Kaleh Bozi’). Results showed that the highest yield in ‘GhermezPeste’ obtained when the manual pollination carried out at the stage that the cluster were green (early flowering period) while the highest yield In ‘KalKhandan’ and ‘KalehBozi’ were observed when the pollination carried out at the full bloom stage. The highest amount of total phenolics, total flavonoid, and antioxidant activity of fruits observed when pollination carried out at the stage that lower florets opened and clusters were green. The Lowest amount of total flavonoid and antioxidant capacity were obtained in the fruits that pollination carried out in the stage that the stigma in middle and lower florets were pink. As a first step towards identifying the best time for manual pollination of pistachio trees, our results showed manual pollination in the suitable blooming stage led to higher yield and quality of pistachio nuts.
Hossein Meighani; Mahmood Ghasemnezhad; Davood Bakshi
Abstract
Aril browning threatens production, consumption, and exports of pomegranates, because affected fruit cannot be externally distinguished from healthy fruit. This study compared the mineral, biochemical composition, and related enzyme activities in affected brown arils with healthy ones in ‘Malase ...
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Aril browning threatens production, consumption, and exports of pomegranates, because affected fruit cannot be externally distinguished from healthy fruit. This study compared the mineral, biochemical composition, and related enzyme activities in affected brown arils with healthy ones in ‘Malase Saveh’ pomegranates. The results indicated that concentrations of Cu in the aril and K, Mg, and Mn in the peel were higher in the healthy fruit than in the affected fruit. The total soluble solids, titratable acidity, total phenolics, total flavonoids, total anthocyanins, antioxidant activity, and color parameters (L*, a*, b*, hue, and chroma) decreased in the browned arils of pomegranates, whereas fruit respiration rate and acidity, peroxidase (POD), and polyphenol oxidase (PPO) enzyme activity were higher in the browned arils. No difference was found for phenylalanine ammonia lyase (PAL) activity. There were positive correlations between total anthocyanins and both color values and total phenols, and a negative correlation between PPO and POD activities was observed. Overall, the nutritional and functional value of the affected fruit is anticipated to be far less than that of the healthy fruit.
Soheila Mohammadrezakhani; Zahra Pakkish
Abstract
Storage of Lime (Citrus aurantifolia L.) fruits, originally a tropical fruits, in low temperature confronts with several difficulties due to the risk of chilling injury (CI). To develop an effective method aiming to reduce CI, the effects of treatments containing 0, 0.5 and 1 Mg/lit brassinosteroids ...
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Storage of Lime (Citrus aurantifolia L.) fruits, originally a tropical fruits, in low temperature confronts with several difficulties due to the risk of chilling injury (CI). To develop an effective method aiming to reduce CI, the effects of treatments containing 0, 0.5 and 1 Mg/lit brassinosteroids (BRs) and hot water (HW) including 20°C as control, 45 and 55°C for 30 minute on CI was studied. Moreover lipid peroxidaion, hydrogen peroxide, catalase (CAT) and peroxidase (POD) activities were investigated in lime fruits stored at 1°C for 21 days. The CI, lipid peroxidaion, hydrogen peroxide were significantly reduced by BRs treatment particularly at 1Mg/lit and 45°CHW treatment. Furthermore fruits treated with 1Mg/lit BRs and 45 °C HW treatment exhibited significantly higher CAT and POD activities in comparison with the control fruits. These results suggest that BRs and HW treatment protect lime fruits from CI by enhancing antioxidant enzymes such as POD and CAT activities, and reduce lipid peroxidaion, hydrogen peroxide contents, and maintaining membrane integrity.
Mina Taghizadeh; Mousa Solgi
Abstract
An important part of plant in vitro techniques is the sterilization of explants and the maintenance of aseptic conditions. Ideally, sterilizing materials should be effective on a vast range of microorganisms at low density. Nowadays, the use of compounds such as essential oils (EOs) and nanoparticles ...
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An important part of plant in vitro techniques is the sterilization of explants and the maintenance of aseptic conditions. Ideally, sterilizing materials should be effective on a vast range of microorganisms at low density. Nowadays, the use of compounds such as essential oils (EOs) and nanoparticles is applicable in microbiology studies. The main objective of this experiment was to study the substitution probability of silver nanoparticles (SNPs), thymol and carvacrol as novel sterilization agents in the tissue culture of Cynodon dactylon. Explants were sterilized with 70% ethanol for 2 min, and then 30% Clorox for 15 min. Sterilization complementary treatments (SNPs, thymol and carvacrol) were applied at different concentrations (100 and 200 mg l-1) with exposure times of 30, 60 and 120 min. According to the results, infection of bermudagrass explants was controlled successfully by SNPs, thymol and carvacrol. Examination of various concentrations in different exposure times showed that 200 mg L-1 SNPs in combination with 100 mg L-1 thymol in 60 min inhibited microbial growth. Thymol and carvacrol were more effective than SNPs in controlling bacteria and fungi contaminations. Finally, these novel agents could be used as an alternative to common chemical treatments for elimination and control of microbial population explants in in vitro conditions.
Nafiseh Geshnizjani; Morteza Khosh-khui
Abstract
Commercially available amino acids mixtures have several advantages such as enhancing assimilation of fertilizer, facilitating uptake of water and nutrients and improving photosynthesis of plants. To investigate the response of gerbera (Gerbera jamesonni L.) flowers ‘Saltino’ to foliar application ...
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Commercially available amino acids mixtures have several advantages such as enhancing assimilation of fertilizer, facilitating uptake of water and nutrients and improving photosynthesis of plants. To investigate the response of gerbera (Gerbera jamesonni L.) flowers ‘Saltino’ to foliar application of a mixture of 19 essential amino acids (0.25, 0.50 and 0.75 mg L-1) and ammonium nitrate (200 mg L-1) as nitrogen source, a pot experiment was carried out in the research greenhouse of Eram Botanical Garden of Shiraz University. Number of flowers, flower diameter, stems length, chlorophyll, proline, protein and nitrogen contents, photosynthesis rate, stomatal conductance, and vase life of flowers were significantly improved by amino acids treatments. No significant difference was observed between ammonium nitrate and amino acids mixture for flower fresh and dry weights. Results suggested that application of amino acids mixture can induce acetyl CoA, which plays an improvingrole in physiological processes in plants. Application of amino acids mixture as a promising and sustainable approach can be recommended to promote quality and quantity of gerbera flowers.
Seyed Mohammad Hassan Mortazavi; Fatemeh Azizollahi; Noorallah Moallemi
Abstract
The fruit of date palm (Phoenix dactylifera L.) is an important horticultural product in the Middle East and North Africa. Among more than 400 reported date palm cultivars in Iran, around 20 cultivars are more important due to having better eating quality and trading values. In this study, the fruit ...
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The fruit of date palm (Phoenix dactylifera L.) is an important horticultural product in the Middle East and North Africa. Among more than 400 reported date palm cultivars in Iran, around 20 cultivars are more important due to having better eating quality and trading values. In this study, the fruit of nine commercially important date cultivars including ‘Barhee’, ‘Breim’, ‘Deiri’, ‘Fersi’, ‘Gantar’, ‘Khadravi’, ‘Sayer’, ‘Shakar’ and ‘Zahidi’ were evaluated for certain quality and biochemical properties during the last three stages of ripening (i.e., Khalal, Rutab and Tamar). The results showed that the highest levels of total soluble solids (TSS) and titratable acidity were recorded at Tamar stage for all studied cultivars. Maximum TSS was recorded for ‘Deiri’ (77 %) and ‘Zahidi’ (75.3%) which are considered as dry dates. By a similar pattern, the mean amount of soluble proteins, flesh darkening and peroxidase activity (POX) increased to 5587 μg g-1, 0.76 A410 and 5220 Ug-1 tissue, respectively. Fruit at Khalal stage showed an incredible amount of phenolic compounds and antioxidant capacity, but these parameters of nutritional value reduced to their lowest level in almost all studied cultivars at Tamar stage. Flesh darkening as an index of appearance quality increased greatly by turning Rutab into Tamar for almost all cultivars.
Ali Tanhaei; Ahmad Reza Dadras; Hossein Sabouri; Ebrahim Gholamalipour Alamdari; Sayed Javad Sajadi; Hossein Hosseini Moghaddam
Abstract
Olive (Olea europaea L.) is a historic and significant Mediterranean tree that has been widely used for its curative properties and oily nature. Images of 150 randomly selected fruits were captured and processed in three replications to investigate the genetic diversity among 98 olive genotypes. The ...
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Olive (Olea europaea L.) is a historic and significant Mediterranean tree that has been widely used for its curative properties and oily nature. Images of 150 randomly selected fruits were captured and processed in three replications to investigate the genetic diversity among 98 olive genotypes. The difference in all traits between genotypes was significant (P˂0.01), indicating a high level of genetic diversity among the olive genotypes. D1 outperformed other genotypes in terms of fruit area, major axis length, convex area, filled area, and equiv. diameter. The major axis length of the fruit exhibited a significant positive correlation with the perimeter, equivalent diameter, major axis length, and area of the stone (P≤0.01). Also, there was a significant, positive correlation between the minor axis lengths of the fruit and stone. The explained percentage of the traits’ associated markers indicated that the fruits’ major axis length had the highest cumulative coefficient (39%) with five bands. The IJS9-A and ScoT21-B genes regulated the most significant number of traits. The former regulated seven characteristics, i.e. fruit area, major axis length, minor axis length, convex area, filled area, equivalent diameter, and perimeter. In comparison, the latter regulated six characteristics, i.e. fruit area, major axis length, convex area, filled area, and the equivalent diameter of the fruit. Cluster analysis was used for categorizing genotypes into two groups. The findings of this study can be applied in hybridization and production programs for developing genotypes with more suitable fruits.