Document Type : Research paper


1 Department of Horticultural Technology, Federal College of Horticulture, Dadin Kowa, Gombe, Nigeria

2 Department of Crop Protection/Institute for Agricultural Research, Ahmadu Bello University Zaria, Nigeria


This study is aimed to detect weed hosts of Tomato Yellow Leaf Curl Virus (TYLCV) in field-grown tomato (Solanum lycopersicum L.) in Sudan savanna regions (Gombe, Jigawa and Kano states) of Nigeria during 2017 and 2018 dry and wet seasons. Three farms each from three Local Government Areas (LGAs) of each state were surveyed. Ten symptomatic and asymptomatic weed samples within and around each farm (n=1080) were randomly collected and assayed against TYLCV using Triple Antibody Sandwich Enzyme-linked Immunosorbent Assay (TAS-ELISA). Based on the obtained results, 14 weed species from 12 families were detected as alternative host of TYLCV in all states surveyed but with variation in distribution. Gombe and Kano states had significantly (p ≤ 0.05) the highest number (7) of TYLCV weed hosts while the lowest number (4) of TYLCV weed hosts was recorded in Jigawa state. Cassia obtusifolia Linn., significantly (p ≤ 0.05) showed the highest TYLCV frequency rating of 12.1%. The study reports for the first time 13 weed species naturally occurring as an alternative host of TYLCV in Nigeria. The detection of these weed hosts of TYLCV will give a better understanding of the virus disease epidemiology for its effective management. Exploring more weed hosts of TYLCV and molecular characterization of the virus in these weeds for the possible evolution of novel strain(s) in the region is recommended.


1. Abraham P, Banwo O.O, Kashina B.D, Alegbejo M.D. 2019b. Detection of alternative hosts of some tomato viruses in Sudan Savanna, Nigeria. A paper presented at the Maiden National Conference of Nigerian Society for Plant Virology held at the Conference centre International Institute of Tropical Agriculture, Ibadan, Oyo State, Nigeria, 28th – 31st October, 2019.
2. Abraham P, Banwo O.O, Kashina B.D, Alegbejo M.D. 2019a. Status of tomato viruses in Nigeria. FUDMA Journal of Sciences 3 (3), 482-494.
3. Alarcón-Flores M.I, Romero-González R, Vidal J.L M, Frenich A.G. 2016. Multiclass Determination of Phenolic Compounds in Different Varieties of Tomato and Lettuce by Ultra High-Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry. International Journal of Food Properties, 19 (3), 494-507.
4. Aguiar R.W.S, Alves G.B, Queiroz A.P, Nascimento I.R, Lima M.F. 2018. Evaluation of weeds as virus reservoirs in watermelon crops. Planta Daninha 36, 1-10.
5. Akobundu I.O, Ekeleme F, Agyakwa C.W, Ogazie C.A. 2016. A handbook of West African weeds (3rd ed.). International Institute of Tropical Agriculture (IITA), Nigeria. 379pp.
6. Alegbejo M.D, Kashina, B.D. 2000. Survey of weed host of blackeye cowpea mosaic and cowpea aphid-borne mosaic potyviruses in Samaru, Nigeria. Journal of Pure and Applied Sciences 3(2):119-125.
7. Asala S, Alegbejo M.D, Kashina B.D, Banwo O.O, Shinggu C.P. 2014. Viruses in weeds in Dioscorea yam fields in Nigeria. African Crop Science Journal 22 (2), 109 – 115.
8. Bello I, Alegbejo M.D, Banwo O.O, Kashina B.D. 2017. Survey for alternative hosts of viruses of
irrigated tomato (Solanum lycopersicum L.) in Sokoto and Zamfara States, Nigeria. A paper presented to at the 42nd annual conference of Nigerian Society for Plant Protection held at the University of Agriculture Makurdi, 12th – 16th March, 2017.
9. Brunt A.A, Crabtree K, Dallwitz M.J, Gibbs A.J, Watson L Zurcher E.J. 1996. Plant Viruses Online: Descriptions and lists from VIDE Database. CAB International, Wallingford, UK, 1484 pp.
10. Chidi A. 2012. Tomato, nutritious vegetable. Seventeen Nigerian Economic Summit. Manufacturing today Nigeria. Alafrican Post Web Team. Pp. 1-3.
11. Clark M.F, Adams A N. 1977. Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34, 475-483.
12. Czosnek H. 2020. Tomato Yellow Leaf Curl Viruses (Geminiviridae). Reference Module in Life Sciences.
13. GEMSA4. 2016. Mapping of Tomato Clusters in Northern Nigeria. Growth and Employment in States – Wholesale and Retail Sector (GEMSA4) Project funded by the DFID/UKAID and the World Bank. St. James House 167 Cadastral Zone Adetokumbo Ademola Crescent Wuse II, Abuja, Nigeria, 38pp., Retrieved on Wednesday, r ectobe05,
2016 , 5:15:24 .MM
14. Gomez K.A, Gomez A.A. 1984. Statistical Procedure for Agricultural Research. 2nd (ed.) Wiley, 680 pp.
15. Huang Y, Li M.Y, Wang F, Xu Z.S, Huang, W, Wang G.L, Ma J, Xiong A.S. 2015. Heat shock factors in carrot: Genome-wide identification, classification, and expression profiles response to abiotic stress. Molecular Biology Reports. (42), 893–905.
16. Kashina B.D. 2017. Begomoviruses in Nigeria. In: Begomoviruses: Occurrence and Management in Asia and Africa. (Saxena S., Tiwari, A. Eds.). Springer, Singapore. 271-283pp.
17. Kashina B.D, Mabagala R.B, Mpunami A.A. 2002. Reservoir weed hosts of Tomato yellow leaf curl begomovirus from Tanzania. Archives of Phytopathology and Plant Protection 35 (4), 269–278.
18. Kashina B.D, Mabagala R.B, Mpunami, A.A. 2003. First report of Ageratum conyzoides L. and
Peter Abraham et al. Int. J. Hort. Sci. Technol. 2021 8(3): 235-246
Sida acuta Burm F. as new weed hosts of Tomato Yellow Leaf Curl Tanzania Virus. Plant Protection Science 39 (1), 18–22.
19. Kumar V. (Ed.) 2009. “Methods for the Diagnosis of Plant Virus Diseases: A Laboratory Manual” Training course on Diagnosis of Mlant Virus Diseases held during 28th April – 10th May, 2009 at IITA, Ibadan, Nigeria. 90pp.
20. Kumar P.L, Cuervo M, Kreuze J.F, Muller G, Kulkarni G, Kumari S.G, Massart S, Mezzalama M, Alakonya A, Muchugi A, Graziosi I, Ndjiondjop M.N, Sharma R, Negawo A.T. 2021. Phytosanitary Interventions for Safe Global Germplasm Exchange and the Prevention of Transboundary Pest Spread: The Role of CGIAR Germplasm Health Units. Plants, 10 (2), 328.
21. Levy D, Lapidot M. 2008. Effect of plant age at inoculation on expression of genetic resistance to Tomato yellow leaf curl virus. Archives of Virology 153, 171–179.
22. Nono-Womdim R. 2003. An overview of major virus diseases of vegetable crop's in Africa and some aspects of their control, pp. 213-232. In: Plant virology in Sub-Saharan Africa, Proceedings of Conference Organized by IITA. J.d'A. Hughes and J.Odu, (eds.). International Institute of Tropical Agriculture, Ibadan, Nigeria.
23. Odedara O.O, Hughes J.d.A, Odebode A.C, Odu B.O. 2008. Multiple virus infection of lablab (Lablab purpureus [L.] Sweet) in Nigeria. Journal of General Pathology 74:322-325.
24. Olaiya C.O. 2011. Bioregulators favourably affect the levels of vitamins and sugars in tomato fruit tissues. Vegetable Crops Research Bulletin (75),71–79
25. Papayiannis L.C, Katis N.I, Idris A.M, Brown J.K. 2011. Identification of weed hosts of Tomato yellow leaf curl virus in Cyprus. Plant Disease.95(2):120-5.
26. Patil B.L. 2020. Plant Viral Diseases: Economic Implications. Reference Module in Life Sciences.
27. Péréfarres F, Thierry M, Becker N, Lefeuvre P, Reynaud B, Delatte H, Lett J.M. 2012. Biological invasions of geminiviruses: case study of TYLCV and Bemisia tabaci in Reunion Island. Viruses. 3665-88.
28. Prasad A, Sharma N, Hari-Gowthem G, Muthamilarasan M, Prasad M. 2020. Tomato Yellow Leaf Curl Virus: Impact, Challenges, and Management. Trends in Plant Science DOI:10.1016/j.tplants.2020.03.015.
29. Sastry K.S, Zitter T.A. 2014. Plant virus and viroid diseases in the tropics: Epidemiology and management. Vol. 2. Springer. 489pp.