Hassan Sarikhani; Hassan Sarikhani-Khorami
Abstract
Cadaman Avimag rootstock is widely used for almonds and peaches. The purpose of this studywas to investigate the effect of light quality on micropropagation, and some morphological andphysiological properties of this rootstock. Single node explants were cultured on WPM and MSmedia containing three levels ...
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Cadaman Avimag rootstock is widely used for almonds and peaches. The purpose of this studywas to investigate the effect of light quality on micropropagation, and some morphological andphysiological properties of this rootstock. Single node explants were cultured on WPM and MSmedia containing three levels of BA (0, 1 and 2 mg L-1) and three levels of NAA (0, 0.05 and0.1 mg L-1). WPM medium containing 2 mg L-1 BA and 0.1 mg L-1 NAA was selected fordifferent light quality treatments including fluorescent, red, and red+blue lights. Results showedthat light quality had a significant effect on shoot proliferation, length and diameter, nodenumber, shoot fresh and dry weights, leaf thickness, stomata number, and chlorophyllconcentration. Red light resulted in higher shoot diameter, but crunchiness and lack of propershoot growth. Moreover, red light produced the lowest amount of chlorophyll in the explants.Leaf thickness and its structural layers under the red light were the lowest among differenttreatments. Combined red+blue light in many factors resembled fluorescent light but inducedmore chlorophyll and a larger stomata size. The quality of light influenced the growth of thisrootstock in vitro, and affected the stem and leaf vegetative traits.
Masoumeh Pourghorban; Pejman Azadi; Shahab Khaghani; Abbas Mirzakhani; Mahdi Changizi; Behzad Edrisi
Abstract
In the commercial production of roses, introducing a method to reduce the time of propagation with maximum success is crucial. This study was conducted to investigate the effect of different concentrations (0, 1500, 3000, 4500 mg/L) of Indole-3-butyric acid (IBA) on propagation of three cultivars of ...
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In the commercial production of roses, introducing a method to reduce the time of propagation with maximum success is crucial. This study was conducted to investigate the effect of different concentrations (0, 1500, 3000, 4500 mg/L) of Indole-3-butyric acid (IBA) on propagation of three cultivars of Rosa hybrid L. ('Dolce Vita', 'Samurai' and 'Utopia') by stenting (simultaneous cutting and grafting) method under greenhouse conditions. The cultivars were grafted onto Rosa hybrida L. 'Natal Briar' rootstock. The factorial experiment was performed in a completely randomized design with three replications. The stentings were cultured in coco peat and perlite medium at 1:2 ratio in a greenhouse under mist system. After 60 days, the percentage of grafting, rooting percentage, root number, longest root length and fresh and dry weights of roots and shoot traits (including leaf number, shoot number, and longest shoot length) were determined on the stentings. The highest rate of rooting in Dolce Vita cultivar was obtained at 1500 mg/L IBA and in Samurai and Utopia cultivars at 4500 mg/L IBA. Among the three cultivars of roses, highest percentage of rooting (93.75%), healing percentage (93.75%), root length (12.47 cm), fresh weight (0.84 g) and dry weight (0.07 g) of roots were observed in stentings treated with 4500 mg/L of IBA in Rosa hybrida L. cv. Samurai. Interaction between IBA concentrations and cultivars on chlorophyll (a, b and total) and carotenoid contents were significant in all the three cultivars of roses.
Mina Farsi; Mohammad Reza Fatahimoghadam; Zabihollah Zamani; Darab Hassani; Ahmad Ahmadi
Abstract
Compared to other techniques of propagation, grafting is the most successful and feasible technique for asexual propagation of walnut plants. There is little information about graft union formation in walnut. Therefore, the objective of this study was to evaluate histological events during graft union ...
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Compared to other techniques of propagation, grafting is the most successful and feasible technique for asexual propagation of walnut plants. There is little information about graft union formation in walnut. Therefore, the objective of this study was to evaluate histological events during graft union formation in Juglans regia L. cv. Chandler scions when minigrafted on the one-year-old seedlings. Cross and longitudinal sections of the graft union were taken for examining different stages of grafting process after 1, 14, 30, 60, 120, 180, 420 and 540 days of minigrafting. One day after grafting, brown necrotic layers were observed at the cut edges. The first callus cells were initiated from cambium layer of rootstock 14 days after grafting but there was weak connection between two parts. New vascular connections between rootstock and scion were observed 30 days after grafting . Vascular connections were increased in central parts of the graft union 60 days after grafting. In mid-summer (180 days after grafting), necrotic layer was almost disappeared in the central longitudinal sections but they were increased especially in the bark, callus and top parts of the graft union due to high temperature and low humidity. In 420 days after grafting, two parts were strongly connected by xylem vessels, but necrotic layer was still remained and observed in some parts. In 540 day after grafting which was coincided with the end of second growing season, two parts were tightly connected to each other and necrotic layer disappeared in most of cross and longitudinal sections.
Seyedeh Faezeh Taghizadeh; Ahmad Asgharzadeh; Javad Asili; Amirhossein Sahebkar; Abolfazl Shakeri
Abstract
Mahaleb (Prunus mahaleb L.) is an important rootstock for P. avium and P. cerasus cultivars. The present study has compared the phenolic contents and antioxidant activity of the methanolic extracts of the barks, leaves and fruits of ten selected mahaleb genotypes. The total phenolic content (5.11-131.77 ...
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Mahaleb (Prunus mahaleb L.) is an important rootstock for P. avium and P. cerasus cultivars. The present study has compared the phenolic contents and antioxidant activity of the methanolic extracts of the barks, leaves and fruits of ten selected mahaleb genotypes. The total phenolic content (5.11-131.77 mg GA g-1) in barks and the total flavonoid (54.06-180.6 mg QE g-1) and proanthocyanidin (8.89-25.33 mg CA g-1) contents in fruits were greater than the other parts of the plants. The maximum contents of total phenol and total proanthocyanidin were in the stem bark and fruit of the genotype '249' (131.77 mg GA g-1, 25.33 mg CA g-1, respectively), while the maximum contents of flavonoid, and anthocyanin were in the fruits of genotype 271 (180.6 mg QE g-1 and 260.81 mg CY g-1, respectively). Antioxidant activity of the samples was determined using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and reducing power assay (RPA). The antioxidant activity was the highest with the genotype '249', which showed 80.9% and 89.3% in DPPH and RPA assays, respectively. This study showed that total phenolic, flavonoid, proanthocyanidin, and anthocyanin contents were affected by mahaleb genotypes. This information may be of assistance in the production of mahaleb genotypes with maximum levels of desired phenolic.
