Document Type : Research paper

Authors

1 Horticulture Crops Research Department, Agriculture and Natural Resources Research Center of Semnan Province (Shahrood), AREEO, Shahrood, Iran

2 Department of Horticulture, Damghan Branch, Islamic Azad University, Damghan, Iran

3 Forests and Rangelands Research Department, Semnan Agricultural and Natural Resources Research and Education Center, AREEO, Semnan, Iran

Abstract

Internal conditions and external display of grafted fruit trees are the results of rootstock effects on nutrient uptake, type and amount of rootstock inductive effects on the scion, and scion consumption. Effect of grafting combination on nutrient content, phenyl propanoid pathway enzymes and their effect on secondary metabolites production of apple leaves was investigated in two consecutive years. Two Iranian red flesh apple cultivars and ‘Red delicious’ cultivar grafted on M9, B9 and one red flesh apple colony rootstock of Bekran were tested in a factorial randomized complete block design. To make a noticeable difference in the amount of nutrients in samples, the treatments in the second year were fertilized. Nitrogen, phosphorus, potassium and calcium were measured in leaves for two years. Also PAL (Phenylalanine ammonia-lyase) and UFGT (UDP-glucose: flavonoid 3-O-glucosyltransferase) enzymes activity, phenolic compounds and flavonoids were determined by high-performance liquid chromatography (HPLC) in vegetative tissues. According to results of this study, among studied rootstocks, M9 was able to accumulate and maintain high concentration of minerals in leaves. Red flesh apples are capable of production of secondary metabolites if they grafted on rootstocks with higher absorption rate. In this way, they can meet the goal of secondary metabolites production. Also, our results showed that calcium was effective in production of all phenolic and flavonoid compounds and induction of UFGT enzyme activity.
Abbreviations: N, Nitrogen; P, Phosphorus; K, Potassium; Ca, Calcium; Fe, Iron; Zn, Zinc; PAL, Phenylalanine ammonia-lyase; UFGT, UDP-glucose: flavonoid 3-O-glucosyltransferase; HPLC, High-performance liquid chromatography; BA, Bastam; BE, Bekran

Keywords

Abdalla O.A, Khatamian H, Miles N.W. 1982. Effect of rootstocks and interstems on composition of ‘Delicious’ apple leaves. Journal of the American Society for Horticultural Science 107,730-733.
Aguirre P.B, Al-Hinai Y.K, Roper T. R, Krueger A. R. 2001. Apple tree rootstock and fertilizer application timing affect nitrogen uptake. HortScience 36, 1202-1205.
Ahenger M.A, Agarwal R.M. 2017. Potassium up-regulates antioxidant metabolism and alleviates growth inhibition under water and osmotic stress in wheat (Triticum aestivum L). Protoplasma 254, 1471-1486.‏
Amiri M.E, Fallahi E. 2009. Potential of Mineral Uptake Efficiency by Some Apple Rootstocks.‏ UC Davis: Departtment of plant science.
Awad M.A, de Jager A, van Westing L.M. 2000. Flavonoid and chlorogenic acid levels in apple fruit: characterisation of variation. Scientia Horticulturae 83, 249-263.‏
Bais H.P, Walker T. S, Schweizer H.P, Vivanco, J.M. 2002. Root specific elicitation and antimicrobial activity of rosmarinic acid in hairy root cultures of Ocimum basilicum.  Plant Physiology and Biochemistry 40, 983–995.
Bate N.J, Orr J, Ni W, Meromi A, Nadler-Hassar T, Doerner P.W, Dixon R.A, Lamb C.J, Elkind Y. 1994. Quantitative relationship between phenylalanine ammonia-lyase levels and phenylpropanoid accumulation in transgenic tobacco identifies a rate-determining step in natural product synthesis. Proceedings of the National Academy of Sciences 91, 7608–7612.
Bryant, J.P, Chapin, F.S, Klein, D. R. 1983. Carbon nutrient balance of boreal plants in relation to vertebrate herbivory. Oikos 40, 357–368.
Bradford, M. M, 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry 72, 248–254.
Bremner J. M. 1996. Nitrogen‐total. Methods of soil analysis: Part 3 Chemical methods 5, 1085-1121.
Chalker-Scott L. 1999. Environmental significance of anthocyanins in plant stress responses, Photochemistry and Photobiology 70, 1–9.
Chang C.C, Yang M. H, Wen H. M, Chern J.C. 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of food and drug analysis 10, 178-182.
Darjazi B.B, Jaimand K. 2017. The Effect of Rootstocks on the Peel Phenolic Compounds of Clementine Mandarin (Citrus clementina). Journal of Medicinal Plants and By-products 6, 173-180.
Davis D.R. 2009. Declining fruit and vegetable nutrient composition: What is the evidence?. HortScience 44, 15-19.‏
Dong S, Cheng L, Fuchigami L.H. 1998.  Nutrient uptake by new roots of six clonal apple rootstocks. HortScience 34,492-493.
El-Beltagi H.S, Mohamed H. I. 2013. Alleviation of cadmium toxicity in Pisum sativum L. seedlings by calcium chloride. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 41, 157-168.‏
Emami A. 1996. Plant decomposition methods. Soil and Water Research Institute.
Fallahi E, Westwood M.N, Chaplin M.H, Richardson D. G. 1984. Influence of apple rootstocks and K and N fertilizers on leaf mineral composition and yield in a high density orchard. Journal of plant nutrition 7, 1161-1177.  
Feyissa A.M, Chandran A, Stockmeier C.A, Karolewicz B. 2009. Reduced levels of NR2A and NR2B subunits of NMDA receptor and PSD-95 in the prefrontal cortex in major depression. Progress in Neuro-Psychopharmacology and Biological Psychiatry  33, 70-75.‏
Foster T.M, McAtee P.A, Waite C.N, Boldingh H.L, McGhie T.K. 2017. Apple dwarfing rootstocks exhibit an imbalance in carbohydrate allocation and reduced cell growth and metabolism. Horticulture research 4, 1-13.
Francisco R.M, Regalado A, Ageorges A, Burla B.J, Bassin B, Eisenach C, Zarrouk O, Vialet S, Marlin T, Chaves M.M, Martinoia E. 2013. ABCC1, an ATP binding cassette protein from grape berry, transports anthocyanidin 3-O-glucosides. The Plant Cell 25, 1840-1854.
 Fritz C, Palacios-Rojas, N, Feil R, Stitt, M. 2006. Regulation of secondary metabolism by the carbon–nitrogen status in tobacco: nitrate inhibits large sectors of phenylpropanoid metabolism. The Plant Journal 46, 533–548.
Ganji Moghadam E, Talaie A, Mokhtarin A. 2007. Relationships between total phenol content, mineral nutrition and vigor of some selected dwarf Iranian mahaleb (Prunus mahaleb L.) genotypes. Journal of Plant Sciences 2, 82-88.
Gerats A.G.M, Bussard J, Coe E.H, Larson R. 1984. Influence of B and pl on UDPG: Flavonoid-3-O-glucosyltransferase in Zea mays L. Biochemical genetics 22, 161–1169.
Gil-Izquierdo A, Riquelme M. T, Porras I, Ferreres F. 2004. Effect of the rootstock and interstock grafted in lemon tree (Citrus limon L. Burm.) on the flavonoid content of lemon juice. Journal of agricultural and food chemistry 52, 324-331.
Gomez C, Terrier N, Torregrosa L, Vialet S, Fournier-Level A, Verries C, Souquet J.M, Mazauric J.P, Klein M, Cheynier V, Ageorges A. 2009. Grapevine MATE-type proteins act as vacuolar H+ - dependent acylated anthocyanin transporters. Plant Physiology 150, 402-415.
Hilbert G, Soyer J, Molot C, Giraudon J. 2003. Effects of nitrogen supply on must quality and anthocyanin accumulation in berries of cv. Merlot. Vitis 42, 69–76.
Ibrahim M.H, Jaafar H.Z, Rahmat A, Rahman Z.A. 2011. Effects of nitrogen fertilization on synthesis of primary and secondary metabolites in three varieties of kacip Fatimah (Labisia pumila Blume). International Journal of Molecular Sciences 12, 5238-5254.‏
Jarrell W.M, Beverly R.B. 1981. The dilution effect in plant nutrition studies. Advances in agronomy 34, 197-224.‏
Jones O.P. 1976. Effect of dwarfing interstocks on xylem sap composition in apple trees: effect on nitrogen, potassium, phosphorus, calcium and magnesium content. Annals of Botany 40, 1231-1235.‏
Ju Z, Liu C, Yuan Y. 1995. Activities of chalcone synthase and UDPGal: flavonoid-3-oglycosyltransferase in relation to anthocyanin synthesis in apple. Scientia Horticulturae 63, 175–185.
Keski‐Saari S, Julkunen‐Tiitto R. 2003. Resource allocation in different parts of juvenile mountain birch plants: effect of nitrogen supply on seedling phenolics and growth. Physiologia Plantarum 118, 114-126.
Kviklys D, Liaudanskas M, Janulis V, Viškelis P, Rubinskienė M, Lanauskas J, Uselis N. 2015. Rootstock genotype determines phenol content in apple fruits. Plant, Soil and Environment 60, 234-240.
Li W, Koike K, Asada Y, Yoshikawa T, Nikaido T. 2005. Rosmarinic acid production by Coleus forskohlii hairy root cultures. Plant cell, tissue and organ culture 80, 151–155.
Mainla L, Moor U, Karp K, Pussa T. 2011. The effect of genotype and rootstock on polyphenol composition of selected apple cultivars in Estonia. Agriculture 98, 63-70.
Mashayekhi K, Sadeghi H, Akbarpour V, Atashi S, Mousavizadeh S.J, Abshaei M, Nazari Z. 2013. Effect of some citrus rootstocks on the amount of biochemical composition of Parson Brown and Mars Oranges in Jiroft. Journal of Horticultral Science 27, 9-17.
Mccallum J.A, Walker J.R. 1990. Phenolic biosynthesis during grain development in wheat: changes in phenylalanine ammonia-lyase activity and soluble phenolic content. Journal of cereal science 11, 35–49.
Parvaneh T, Abedi B, Davarynejad G, Ganji Moghadam E. 2019. Correlation of Nutrient Content, Activity of Phenylpropanoid Pathway Enzymes and Secondary Metabolites in Two Iranian Red Flesh Apple Genotypes and Red delicious Cultivar on Different Rootstocks. Journal of Crops Improvement 22, 149-163.
Ruhmann S, Treutter D. 2003. Effect of N-nutrition in apple on the response of its secondary metabolism to prohexadione-Ca treatment. European Journal of Horticultural Science 68, 152-159.‏
Satisha J, Prakash G.S, Murti G.S.R, Upreti, K.K. 2005. Response of grape genotypes to water deficit: Root, shoot, growth and endogenous hormones. Indian journal of plant physiology 10, 225- 230.‏
Scalzo J, Politi A, Pellegrini N, Mezzetti B, Battino M. 2005. Plant genotype affects total antioxidant capacity and phenolic contents in fruit. Nutrition 21, 207-213.
Sobhana P, Thomsa M, Krishnakumar R, Jacob J. 2000. Can there be possible genetic conflict between genetically divergent rootstocks and scions in bud grafted plants. National Symposium on Recent Trends in Plant Science Research, University of Kerala, Trivandrum, India.‏
Steyn W.J, Wand S.J.E, Holcroft D.M, Jacobs G. 2002. Anthocyanins in vegetative tissues: a proposed unified function in photoprotection. New Phytologist 155, 349-361.‏  
Talukdar D. 2012. Exogenous calcium alleviates the impact of cadmium-induced oxidative stress in Lens culinaris Medic. Seedlings through modulation of antioxidant enzyme activities. Journal of Crop Science and Biotechnology 15, 325-334.‏
Tomar N.S, Agarwal R.M. 2013. Influence of treatment of Jatropha curcas L. leachates and potassium on growth and phytochemical constituents of wheat (Triticum aestivum L.).‏ American Journal of Plant Sciences 4, 1134–1150.
Treutter D, eucht W. 1987. Accumulation of the flavonoid prunin in Prunus avium‏/P. cerasus grafts and its possible involvement in the process of incompatibility. nternational Symposium on Vegetative Propagation of Woody Species 227, 74-78.
Tsao R, Yang R, Young J.C, Zhu H. 2003. Polyphenolic profiles in eight apple cultivars using high-performance liquid chromatography (HPLC). Journal of agricultural and food chemistry 51, 6347-6353.
Usenik V, Stampar F. 2000. Influence of various rootstocks for cherries on p-coumaric acid, genistein and prunin content and their involvement in the incompatibility process. Gartenbauwissenschaft 65, 245-250.
Xu W. Peng H, Yang T, Whitaker B, Huang L, Sun J, Chen P. 2014. Effect of calcium on strawberry fruit flavonoid pathway gene expression and anthocyanin accumulation. Plant physiology and biochemistry 82, 289-298.‏
Zucker M. 1965. Induction of phenylalanine deaminase by light and its relation to chlorogenic acid synthesis in potato tuber tissue. Plant Physiology 40, 779–784.