Document Type : Research paper


1 Department of Horticulture, Arak Branch, Islamic Azad University, Arak, Iran

2 Department of Genetic Engineering, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), P. O. Karaj, Iran Ornamental Plants Research Center, Horticultural Science Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Mahallat, Iran

3 Department of Genetic and Plant Breeding, Arak Branch, Islamic Azad University, Arak, Iran

4 Horticulture Crops Research Department, Markazi Agricultural and Natural Resources Research and Education center, AREEO, Arak, Iran

5 Ornamental Plants Research Center, Horticultural Science Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Mahallat, Iran


In the commercial production of roses, introducing a method to reduce the time of propagation with maximum success is crucial. This study was conducted to investigate the effect of different concentrations (0, 1500, 3000, 4500 mg/L) of Indole-3-butyric acid (IBA) on propagation of three cultivars of Rosa hybrid L. ('Dolce Vita', 'Samurai' and 'Utopia') by stenting (simultaneous cutting and grafting) method under greenhouse conditions. The cultivars were grafted onto Rosa hybrida L. 'Natal Briar' rootstock. The factorial experiment was performed in a completely randomized design with three replications. The stentings were cultured in coco peat and perlite medium at 1:2 ratio in a greenhouse under mist system. After 60 days, the percentage of grafting, rooting percentage, root number, longest root length and fresh and dry weights of roots and shoot traits (including leaf number, shoot number, and longest shoot length) were determined on the stentings. The highest rate of rooting in Dolce Vita cultivar was obtained at 1500 mg/L IBA and in Samurai and Utopia cultivars at 4500 mg/L IBA. Among the three cultivars of roses, highest percentage of rooting (93.75%), healing percentage (93.75%), root length (12.47 cm), fresh weight (0.84 g) and dry weight (0.07 g) of roots were observed in stentings treated with 4500 mg/L of IBA in Rosa hybrida L. cv. Samurai. Interaction between IBA concentrations and cultivars on chlorophyll (a, b and total) and carotenoid contents were significant in all the three cultivars of roses.


1. Akinyele A.O. 2010. Effects of Growth Hormones, Rooting Media and Leaf Size on Juvenile Stem Cuttings of Buchholzia coriacea Engler. Annals of Forest Research 53: 127-123. 2. Azadi, P., Beyrami Zadeh, E. and Otang Ntui, V., 2013. A simple protocol for somatic embryogenesis in Rosa hybrida L. cv. Apollo. The Journal of Horticultural Science and Biotechnology, 88(4), pp.399-402.
3. Babaie H. Zarei H. and Hemmati K. 2014. Propagation of Ficus benjamina var. Starlight by Stenting Technique under Different Concentrations of IBA in Various Times of Taking Cutting. Journal of Ornamental Plants 4(2): 75-79.
4. Bekhradi F. Kashi A. and Delshad M. 2011. “Effect of Three Cucurbits Rootstocks on Vegetative and Yield of “Charleston Gray” Watermelon,” International Journal of Plant Production 5(2): 105-110.
5. Dawa S. Rather Z.A. Sheikh M.Q. Nelofar I.T. and Nazki A. H. 2013. Influence of Growth Regulators on Rhizogenesis in Semi Hardwood Cuttings of Some Flower Roses. Journal of Applied Biology and Biotechnology 15(2):1-6.
6. Fishel F. M. 2009. Plant Growth Regulators,
7. Gudin S. 2003. Breeding. In: Roberts A.V., Debener, T. and Gudin, S. (eds). Encyclopedia of Rose Science. Elsevier, Academic, Oxford, UK pp 25-30.
8. Hanan J.J. Grueber K.L. 1987. Understocks. In: Langhans, R.W. (Ed.), Roses. Roses Incorporated, Haslett, MI pp. 29–34.
Propagation of Three Cultivars of Rosa hybrida L. through Stenting Method 35
9. Hartmann H.T. Kester D.E. and Davies Jr. F.T. 1990. Plant Propagation: Principles and Practices, 5th edition. Prentice-Hall, Inc. Englewood Cliffs, New Jersey, USA pp. 647.
10. Hartmann H.T. Kester D.E. Davies F.T. and Geneve R.L. 1997. Plant Propagation: Principles and Practices. Prentice-Hall, Inc., Englewood Cliffs, New Jersey. Sixth edition.
11. Hartmann H.T. Kester D.E. Davies Jr. F.T. and Geneve R.L. 2002. Plant propagation: Principles and Practices. 5th Ed, Prentice-Hall Inc. Englewood, Cliffs, NJ., USA p.647.
12. Hartmann H.T. Kester D.E. Davies F.T. and Geneve R. 2010. Hartmann and Kester’s plant propagation: Principles and practices, In: Hartmann, H.T. Kester, D.E. Davies, F.T. and Geneve, R. (eds.). Principles of grafting and budding. 8th ed. Pearson Higher Education, Harlow, UK p. 425.
13. Islam M.N. Rahim M.A. Naher M.N.A. Azad M.L. and Shahjahan M. 2004. Effect of Time of Operation and Age of Rootstock on the Success of Inserted Contact Grafting in Mango. Asian Journal of Plant Sciences 3(5): 636-641.
14. Izadi Z. 2012. An Investigation of the Effect of Grafting Time, Technique, Kind of rootstock and scion on the success of stenting method of greenhouse rose (R. hybrida). M.Sc. Thesis, Gorgan University of Agricultural Sciences & Natural Resources, Gorgan.
15. Izadi Z. Zarei H. and Alizadeh M. 2013. Role of Grafting Technique on the Success of Stenting Propagation of Two Rose (Rosa sp.) Varieties. American Journal of Plant Sciences 4: 41-44. 2013.45A006.
16. Kasim N.E. and Rayya A. 2009. Effect of Different Collection Times and Some Treatments on Rooting and Chemical Interminal Constituents of Bitter Almond Hard wood Cutting. Journal of Agriculture and Biological Sciences 5(2):116-122.
17. Kazankaya A. Mehmet S. and Tekintas F.G. 1997. Relations between Graft Success and Structural Hormones on Walnut (Juglans regia L.). International Society for Horticultural Science 442: 295-298.
18. Kazankaya A. Yoruk E. Dogan A. 2005. Effect of IBA on Rooting of Rosa canina Hardwood Cuttings from Lake Van Region, Turkey. International Society for Horticultural Science 690:153-158.
19. Khan M.A. Khurram Z. Iftikhar A. 2004. Effect of Various Hormones and Different Rootstocks
on Rose Propagation. Pakistan Journal of Biological Sciences 7:1643-1646.
20. Khosh-Khui M. Teixeira da Silva J.A. 2006. In Vitro Culture of Rosa Species. In: Teixeira da Silva, J.A. (Ed.), Floriculture, Ornamental and Plant Biotechnology, Advances and Topical Issues, vol. 2. Global Science Books, Ltd., UK pp. 516–526.
21. Lichtenthaler H K. 1987. Chlorophylls and Carotenolds: Pigments of Photosynthesis, Methods of Biochemical Analysis 148: 350-352.
22. Majeed M. Khan M.A. and Mughal A.H. 2009. Vegetative Propagation of Aesculus indica through Stem Cuttings Treated with Plant Growth Regulators. Journal of Forest Research 20: 171-173.
23. Mercurio G. 2007. Cut Rose Cultivation Around the World. Http://
24. Naier A. Zhang D. and Smagula J. 2008. Rooting and Overwintering Stem Cuttings of Stewartia pseudocamellia Maxim. Relevant to Hormone, Media, and Temperature. American Society for Horticultural Science 43(7):2124-2128.
25. Nasri F. Fadakar A. Koshesh Saba M. and Yousefi B. 2015. Study of Indole Butyric Acid (IBA) Effects on Cutting Rooting Improving some of Wild Genotypes of Damask Roses (Rosa damascena Mill.). Journal of Agricultural Sciences 60(3): 263-275. DOI: 10.2298/JAS1503263N.
26. Nazari F. Khosh-Khui M. and Salehi H. 2009. Growth and Flower Quality of Four Rosa hybrida L. Cultivars in Response to Propagation by Stenting or Cutting in Soilless Culture. Scientia Horticulturae 119:302-305.
27. Pourghorban M. Khaghani S. Azadi P. Mirzakhani A. and Changizi M. 2019. Propagation of Rosa hybrida L. cv. 'Dolce Vita' by Stenting and Stem Cutting Methods in Response to Different Concentrations of IBA. Advances in Horticultural science 33(1): 105-111.
28. Salehi H. Khosh-Khui M. 1997. Effects of Explant Length and Diameter on in Vitro Shoot Growth and Proliferation Rate of Miniature Roses. Journal Horticultural Science 72: 673–676.
29. Senapati S.K. and Rout G.R. 2008. Study of Culture Conditions for Improved
36 Int. J. Hort. Sci. Technol; Vol. 7, No. 1; March 2020
Micropropagation of Hybrid Rose. Horticultural Science 35: 27-34.
30. Sharma S.K. and Verma S.K. 2011. Seasonal Influences on the Rooting Response of Chir pine (Pinus roxburghii Sarg.). Forestry Journal at Forest Research and Management Institute 54(2): 241-247.
31. Soleimani A. Rabiei, V. and Hassani D. 2010. Effect of Different Techniques on Walnut (J. regia) Grafting,” Journal of Food, Agriculture & Environment 8(29): 544-546.
32. Van de Pol P. A. and Breukel A. 1982. Agricultural University, Department of Horticulture, Wageningen (The Netherlands). Scientia Horticulturae 17: 187-196.
33. Van de Pol P.A. Joosten M.H.A.J. and Keizer H. 1986. Stenting of roses, starch depletion and accumulation during the early development. Acta Hortic 189:51-59.
34. Van de Pol P.A. Pierik R.L.M. 1995. Newest Developments in Rose (Rosa hybrida L.) Propagation. Revista Chapingo 3: 15–22.