Hassan Sarikhani; Mohammad-Sadegh Safariyan-Nejad
Abstract
One of the most important problems of strawberry cultivation in temperate regions is winter cold injuries. Current study investigated impacts of foliar application of glycine betaine (GB) at 0, 0.5, 1, 2 and 4 mM concentrations on the cold hardiness of strawberry. The plants were divided into two groups: ...
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One of the most important problems of strawberry cultivation in temperate regions is winter cold injuries. Current study investigated impacts of foliar application of glycine betaine (GB) at 0, 0.5, 1, 2 and 4 mM concentrations on the cold hardiness of strawberry. The plants were divided into two groups: one group for evaluation of cold hardiness at temperatures of -6, -9, -12, -15 and -18 °C; and the other for study of some biochemical characteristics. Results showed that GB treatment increased soluble carbohydrate and proline concentrations in both leaf and crown tissues, total protein concentration in leaf, and relative water content in leaf as compared to those in control. Based on LT50 calculated from electrolyte leakage and tetrazolium staining test, the GB application increased cold hardiness in strawberry plant based on its concentration. The highest cold hardiness was found in the 2 mM GB concentration based on electrolyte leakage and tetrazolium staining tests at -13.3 and -15.3 °C. Meanwhile, the lowest values of cold hardiness were observed in the control treatments based on electrolyte leakage and tetrazolium staining tests at -10.2 and -11.0 °C. Significant correlations were found between soluble carbohydrate and proline concentrations in leaf and crown, and total protein concentration with LT50 calculated from electrolyte leakage and tetrazolium staining test. We conclude that application of 2 mM GBhas the capacity to increase the freezing tolerance of strawberry and could be used as a prophylactic tool to reduce winter cold injury.
Hassan Sarikhani; Hassan Sarikhani-Khorami
Abstract
Cadaman Avimag rootstock is widely used for almonds and peaches. The purpose of this studywas to investigate the effect of light quality on micropropagation, and some morphological andphysiological properties of this rootstock. Single node explants were cultured on WPM and MSmedia containing three levels ...
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Cadaman Avimag rootstock is widely used for almonds and peaches. The purpose of this studywas to investigate the effect of light quality on micropropagation, and some morphological andphysiological properties of this rootstock. Single node explants were cultured on WPM and MSmedia containing three levels of BA (0, 1 and 2 mg L-1) and three levels of NAA (0, 0.05 and0.1 mg L-1). WPM medium containing 2 mg L-1 BA and 0.1 mg L-1 NAA was selected fordifferent light quality treatments including fluorescent, red, and red+blue lights. Results showedthat light quality had a significant effect on shoot proliferation, length and diameter, nodenumber, shoot fresh and dry weights, leaf thickness, stomata number, and chlorophyllconcentration. Red light resulted in higher shoot diameter, but crunchiness and lack of propershoot growth. Moreover, red light produced the lowest amount of chlorophyll in the explants.Leaf thickness and its structural layers under the red light were the lowest among differenttreatments. Combined red+blue light in many factors resembled fluorescent light but inducedmore chlorophyll and a larger stomata size. The quality of light influenced the growth of thisrootstock in vitro, and affected the stem and leaf vegetative traits.
