Effects of Supplemental Light Quality at The End of Day on Herb Production and Some Phytochemical Properties of Lemon Balm (Melissa officinalis L.)

Document Type : Research paper

Authors

Department of Horticultural Science, Bu-Ali Sina University, Hamedan, Iran

Abstract

Increasing day length during the short photoperiod in fall and winter is a beneficial method of increasing biomass production and altering plant morphology and phytochemistry. The objective of this study was to examine the effects of light quality at the end of the day (EoD) on the growth and phytochemical characteristics of lemon balm. During shortday photoperiods in autumn, lemon balm (Melissa officinalis L.) seedlings were exposed to red, blue, and combined red/blue light using light-emitting diodes for 2 hours at the EoD. The results showed that exposure to red light significantly increased biomass. Plants grown under blue light yielded the highest percentage of dry matter and their leaves had the highest chlorophyll content and flavonoids. The highest carotenoid content was found in plants irradiated with blue light and later with red+blue light. The highest levels of total phenols, anthocyanins, and antioxidant activity were found in plants grown under red light. In addition, light quality had a significant effect on essential oil content. The highest essential oil content was obtained in the red and red+blue light treatments. The light quality at the EoD significantly changed the essential oil composition. The blue light significantly increased the citronellal content but decreased the geranial and linalool content. This study provided insights into the effects of EoD light quality on plant growth and metabolite accumulation in lemon balm with a short photoperiod. In conclusion, supplemental light at the EoD can effectively improve plant growth and secondary metabolite quality in medicinal plants. 

Keywords

References

Adorjan B, Buchbauer G. 2010. Biological properties of essential oils: an updated review. Flavour and Fragrance Journal 25, 407-426. https://doi.org/10.1002/ffj.2024
Ali M, Abbasi BH. 2014. Light-induced fluctuations in biomass accumulation, secondary metabolites production and antioxidant activity in cell suspension cultures of Artemisia absinthium L. Journal of Photochemistry and Photobiology B: Biology 140, 223- 227. https://doi.org/10.1016/j.jphotobiol.2014.08.008
Baek GY, Kim MH, Kim CH, Choi EG, Jin BO, Son JE, Kim HT. 2013. The effect of LED light combination on the anthocyanin expression of lettuce. IFAC Proceedings Volumes 46(4), 120-123. https://doi.org/10.3182/20130327-3-JP-3017.00029
Bajaj KL, Devsharma AK. 1977. A colorimetric method for the determination of tannins in tea. Microchimica Acta 68, 249-253. https://doi.org/10.1007/BF01196209
Bantis F, Ouzounis T, Radoglou K. 2016. Artificial LED lighting enhances growth characteristics and total phenolic content of Ocimum basilicum, but variably affects transplant success. Scientia Horticulturae 198, 277-283. https://doi.org/10.1016/j.scienta.2015.11.014
Barro F, De La Haba P, Maldonado JM, Fontes AG. 1989. Effect of light quality on growth, contents of carbohydrates, protein and pigments, and nitrate reductase activity in soybean plants. Journal of Plant Physiology 134(5), 586-591. https://doi.org/10.1016/S0176-1617(89)80152-X
Batchauer A, Bonerjee P, Pokorny R. 2007.Cryptochromes. In: Whitelam GC, Halliday KJ (Eds), Light and Plant Development. Wiley-Blackwell. pp 17- 48.
Bedreag CFG, Trifan A, Bucur LA, Arcus M, Tebrencu C, Miron A, Costache II. 2014. Chemical and antioxidant studies on Crataegus pentagyna leaves and flowers. Romanian Biotechnological Letters 19(6), 9859-9867.
Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248-254. https://doi.org/10.1016/0003-2697(76)90527-3
Brand-Williams W, Cuvelier ME, Berset C. 1995. Use of a free radical method to evaluate antioxidant activity. Journal of Food Science and Technology 28, 25–30. https://doi.org/10.1016/S0023-6438(95)80008-5
Brown CS, Schuerger AC, Sager JC. 1995. Growth and photomorphogenesis of pepper plants under red lightemitting diodes with supplemental blue or far-red lighting. Journal of the American Society for Horticultural Science 120, 808-813. https://doi.org/10.21273/JASHS.120.5.808
Carnat AP, Carnat A, Fraisse D, Lamaison JL. 1998. The aromatic and polyphenolic composition of lemon balm (Melissa officinalis L. subsp. officinalis) tea. Pharmaceutica Acta Helvetiae 72, 301-305. https://doi.org/10.1016/S0031-6865(97)00026-5
Castleman M. 2010. The new healing herbs: the essential guide to more than 125 of nature’s most potent herbal remedies. 3rd Ed. Rodale Books. USA. pp 300-303.
Chang CC, Yang MH, Wen HM, Chern JC. 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis 10, 178-182.
Chen XL, Guo WZ, Xue XZ, Wang LC, Qiao XJ. 2014. Growth and quality responses of green oak leaf lettuce as affected by monochromic or mixed radiation provided by fluorescent lamp (FL) and light-emitting diode (LED). Scientia Horticulturae 172, 168-175. https://doi.org/10.1016/j.scienta.2014.04.009
Chen XL, Xue XZ, Guo WZ, Wang LC, Qiao XJ. 2016. Growth and nutritional properties of lettuce affected by mixed irradiation of white and supplemental light provided by light-emitting diode. Scientia Horticulturae 200, 111-118. https://doi.org/10.1016/j.scienta.2016.01.007
Choi HG, Moon BY, Kang NJ. 2015. Effects of LED light on the production of strawberry during cultivation in a plastic greenhouse and in a growth chamber. Scientia Horticulturae 189, 22-31. https://doi.org/10.1016/j.scienta.2015.03.022
Cruces E, Rautenberger R, Rojas-Lillo Y, Cubillos VM, Arancibia-Miranda N, Ramírez-Kushel E, Gómez I. 2017. Physiological acclimation of Lessonia spicata to diurnal changing PAR and UV radiation: differential regulation among down-regulation of photochemistry, ROS scavenging activity and phlorotannins as major photoprotective mechanisms. Photosynthesis Research 131, 145–157. https://doi.org/10.1007/s11120-016-0304-4
Da Silva S, Sato A, Lage CLS, San Gil, RAdS, Azevedo Dd A, Esquibel MA. 2005. Essential oil composition of Melissa officinalis L. In vitro produced under the influence of growth regulators. Journal of the Brazilian Chemical Society 16, 1387–1390. http://dx.doi.org/10.1590/S0103-50532005000800014
Darko E, Heydarizadeh P, Schoefs B, Sabzalian MR. 2014. Photosynthesis under artificial light: the shift in primary and secondary metabolism. Philosophical Transactions of the Royal Society of London Biology Science 369(1640), 20130243. https://doi.org/10.1098/rstb.2013.0243
De Sousa AC, Alviano DS, Blank AF, Alves PB, Alviano CS, Gattass CR. 2004. Melissa officinalis L. essential oil: anti-tumoral and antioxidant activities. Journal of Pharmacy and Pharmacology 56, 677-681. https://doi.org/10.1211/0022357023321
Dousti M, Ramchandani MH, Barkhordarian A, Danaei S, Chiappelli F. 2012. Evidence-based traditional Persian medicine. In: Rastogi S, Chiappelli F, Ramchandani MH, Singh RH (Eds.). Evidence-based practice in complementary and alternative medicine: perspectives, protocols, problems, and potential in Ayurveda. Berlin: Springer. p. 88.
Esmaeili S, Aliniaeifard S, Dianati Daylami S, Karimi S, Shomali A, Didaran F, Telesiński A, Sierka E, Kalaji HM. 2022. Elevated light intensity compensates for nitrogen deficiency during chrysanthemum growth by improving water and nitrogen use efficiency. Scientific Reports 12, 10002. https://doi.org/10.1038/s41598-022-14163-4
Estell RE, Fredrickson EL, James DK. 2016. Effect of light intensity and wavelength on concentration of plant secondary metabolites in the leaves of Flourensia cernua. Biochemical Systematics and Ecology 65, 108- 114. https://doi.org/10.1016/j.bse.2016.02.019
Fan XX, Xu ZG, Liu XY, Tang CM, Wang LW, Han XL. 2013. Effects of light intensity on the growth and leaf development of young tomato plants grown under a combination of red and blue light. Scientia Horticulturae 153, 50-55. https://doi.org/10.1016/j.scienta.2013.01.017
Heo JW, Lee CW, Murthy HN, Paek KY. 2003. Influence of light quality and photoperiod on flowering of Cyclamen persicum Mill. cv. Dixie White. Plant Growth Regulation 40, 7-10. https://doi.org/10.1023/A:102309690
Hernandez R. 2013. Growth and development of greenhouse vegetable seedlings under supplemental LED lighting. Ph.D. dissertation, University of Arizona. p. 127.
Hogewoning SW, Trouwborst G, Maljaars H, Poorter H, van Leperen W, Harbin-son J. 2010. Blue light doseresponses of leaf photosynthesis, morphology, and chemical composition of Cucumis sativus grown underdifferent combinations of red and blue light. Journal of Experimental Botany 61(11), 3107–3117. https://doi.org/10.1093/jxb/erq132
Hosseini A, Zare Mehrjerdi M, Aliniaeifard S. 2018. Alteration of bioactive compounds in two varieties of basil (Ocimum basilicum) grown under different light spectra. Journal of Essential Oil Bearing Plants 21(4) 913-923. https://doi.org/10.1080/0972060X.2018.1526126
Huché-Thélier L, Crespel L, Le Gourrierec J, Morel P, Sakr S, Leduc N. 2016. Light signaling and plant responses to blue and UV radiations-perspectives for applications in horticulture. Environmental and Experimental Botany 121, 22-38. https://doi.org/10.1016/j.envexpbot.2015.06.009
Islam MA, Kuwar G, Clarke JL, Blystad DR, Gislerød HR, Olsen JE, Torre S. 2012. Artificial light from light emitting diodes (LEDs) with a high portion of blue light results in shorter poinsettias compared to high pressure sodium (HPS) lamps. Scientia Horticulturae 147, 136-143. https://doi.org/10.1016/j.scienta.2012.08.034
Islam MA, Tarkowská D, Clarke JL, Blystad D, Gislerød HR, Torre S, Olsen JE. 2014. Impact of end-of-day red and far-red light on plant morphology and hormone physiology of poinsettia. Scientia Horticulturae 174, 77-86. https://doi.org/10.1016/j.scienta.2014.05.013
Javadi Asayesh E, Aliniaeifard S, Askari N, Roozban MR, Sobhani M, Tsaniklidis G, Woltering EJ, Fanourakis D. 2021. Supplementary light with increased blue fraction accelerates emergence and improves development of the inflorescence in Aechmea, Guzmania and Vriesea. Horticulturae 7(11), 485. https://doi.org/10.3390/horticulturae7110485
Johkan M, Shoji K, Goto F, Hashida SN, Yoshihara T. 2010. Blue light-emitting diode light irradiation of seedlings improves seedling quality and growth after transplanting in red leaf lettuce. HortScience 45(12), 1809-1814. https://doi.org/10.21273/HORTSCI.45.12.1809
Karimi, M., Ahmadi, N., Ebrahimi, M. 2022. Red LED light promotes biomass, flowering and secondary metabolites accumulation in hydroponically grown Hypericum perforatum L. (cv. ‘Topas’). Industrial Crops and Products 175, 114239. https://doi.org/10.1016/j.indcrop.2021.114239
Kondo S, Tomiyama H, Rodyoung A, Okawa K, Ohara H, Sugaya S, Terahara N, Hirai N. 2014. Abscisic acid metabolism and anthocyanin synthesis in grape skin are affected by light emitting diode (LED) irradiation at night. Journal of Plant Physiology 171(10), 823-829. https://doi.org/10.1016/j.jplph.2014.01.001
Kozai T. 2016. Why LED lighting for urban agriculture? In: LED lighting for urban agriculture (pp. 3-18). Springer, Singapore.
Lange H, Shropshire W, Mohr H. 1971. An analysis of phytochrome-mediated anthocyanin synthesis. Plant Physiology 47, 649-655. https://doi.org/10.1104/pp.47.5.649
Lee SW, Seo JM, Lee MK, Chun JH, Antonisamy P, Arasu MV, Suzuci T, Al-Dhabi NA, Kim SJ. 2014. Influence of different LED lamps on the production of phenolic compounds in common and Tartary buckwheat sprouts. Industrial Crops and Products 54, 320-326. https://doi.org/10.1016/j.indcrop.2014.01.024
Li H, Tang C, Xu Z. 2013. The effects of different light qualities on rapeseed (Brassica napus L.) plantlet growth and morphogenesis in vitro. Scientia Horticulturae 150, 117-124. https://doi.org/10.1016/j.scienta.2012.10.009
Li Q, Kubota C. 2009. Effects of supplemental light quality on growth and phytochemicals of baby leaf lettuce. Environmental and Experimental Botany 67, 59-64. https://doi.org/10.1016/j.envexpbot.2009.06.011
Liu Y, Wang T, Fang S, Zhou M, Qin J. 2018. Responses of morphology, gas exchange, photochemical activity of photosystem II, and antioxidant balance in Cyclocarya paliurus to light spectra. Frontiers in Plant Science 9, 1704. https://doi.org/10.3389/fpls.2018.01704
Liu Y, Schouten RE, Tikunov Y, Liu X, Visser RG, Tan F, Bovy A, Marcelis LF. 2022. Blue light increases anthocyanin content and delays fruit ripening in purple pepper fruit. Postharvest Biology and Technology 192, 112024. https://doi.org/10.1016/j.postharvbio.2022.112024
Matsuda R, Yamano T, Murakami K, Fujiwara K. 2016. Effects of spectral distribution and photosynthetic photon flux density for overnight LED light irradiation on tomato seedling growth and leaf injury. Scientia Horticulturae 198, 363-369. https://doi.org/10.1016/j.scienta.2015.11.045
Mencherini T, Picerno P, Scesa C, Aquino R. 2007. Triterpene, antioxidant and antimicrobial compounds from Melissa officinalis. Journal of Natural Products 70, 1889-1894. https://doi.org/10.1021/np070351s
Mulas G, Gardner Z, Craker LE. 2006. Effect of light quality on growth and essential oil composition in rosemary. Acta Horticulturae 723, 427-432. https://doi.org/10.17660/ActaHortic.2006.723.60
Nagano S, Mori N, Tomari Y, Mitsugi N, Deguchi A, Kashima M, Tezuka A, Nagano AJ, Usami H, Tanabata T, Watanabe H. 2022. Effect of differences in light source environment on transcriptome of leaf lettuce (Lactuca sativa L.) to optimize cultivation conditions. PLOS One 17(3), e0265994. https://doi.org/10.1371/journal.pone.0265994
Namdar D, Charuvi D, Ajjampura V, Mazuz M, Ion A, Kamara I, Koltai H. 2019. LED lighting affects the composition and biological activity of Cannabis sativa secondary metabolites. Industrial Crops and Products 132, 177-185. https://doi.org/10.1016/j.indcrop.2019.02.016
Ouzounis T, Fretté X, Rosenqvist E, Ottosen CO. 2014. Spectral effects of supplementary lighting on the secondary metabolites in roses, Chrysanthemums, andCampanulas. Journal of Plant Physiology 171(16), 1491-1499. https://doi.org/10.1016/j.jplph.2014.06.012
Paquin R, Lechasseur P. 1979. Observations sur une méthode de dosage de la proline libre dans les extraits de plantes. Canadian Journal of Botany 57, 1851–1854. (In French.) https://doi.org/10.1139/b79-233
Piovene C, Orsini F, Bosi S, Sanoubar R, Bregola V, Dinelli G, Gianquinto G. 2015. Optimal red: blue ratio in led lighting for nutraceutical indoor horticulture. Scientia Horticulturae 193, 202-208. https://doi.org/10.1016/j.scienta.2015.07.015
Porra RJ, Thompson WA, Kriedemann PE. 1989. Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochimica et Biophysica Acta 975(3), 384-394.
Qian H, Liu T, Deng M, Miao H, Cai C, Shen W, Wang Q. 2016. Effects of light quality on main health-promoting compounds and antioxidant capacity of Chinese kale sprouts. Food Chemistry 196, 1232-1238. https://doi.org/10.1016/j.foodchem.2015.10.055
Rajapakse NC, Shahak Y. 2007. Light-quality manipulation by horticulture industry. In: Whitelam GC, Halliday KJ (Eds), Light and Plant Development. Wiley-Blackwell. pp 290-310.
Rapisarda P, Fanella F, Maccarone E. 2000. Reliability of analytical methods for determining anthocyanins in blood orange juices. Journal of Agricultural and Food Chemistry 48(6), 2249-2252. https://doi.org/10.1021/jf991157h
Ren J, Guo S, Xu C, Yang C, Ai W, Tang Y, Qin L. 2014. Effects of different carbon dioxide and LED lighting levels on the anti-oxidative capabilities of Gynura bicolor DC. Advances in Space Research 53(2), 353- 361. https://doi.org/10.1016/j.asr.2013.11.019
Sabzalian MR, Heydarizadeh P, Zahedi M, Boroomand A, Agharokh M, Sahba MR, Choefs B. 2014. High performance of vegetables, flowers, and medicinal plants in a red-blue LED incubator for indoor plant production. Agronomy for Sustainable Development 34(4), 879-886. https://doi.org/10.1007/s13593-014-0209-6
Samuolienė G, Brazaitytė A, Urbonavičiūtė A, Šabajevienė G, Duchovskis P. 2010. The effect of red and blue light component on the growth and development of frigo strawberries. Zemdirbyste-Agriculture 97(2), 99-104.
Samuolienė G, Sirtautas R, Brazaitytė A, Duchovskis P. 2012. LED lighting and seasonality effects antioxidant properties of baby leaf lettuce. Food Chemistry 134(3), 1494-1499. https://doi.org/10.1016/j.foodchem.2012.03.061
Seo JM, Arasu MV, Kim YB, Park SU, Kim SJ. 2015. Phenylalanine and LED lights enhance phenolic compound production in Tartary buckwheat sprouts. Food Chemistry 177, 204-213. https://doi.org/10.1016/j.foodchem.2014.12.094
Singleton VL, Rossi JA. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture 16(3), 144-158.
Son YJ, Park JE, Kim J, Yoo G, Nho CW. 2021. The changes in growth parameters, qualities, and chemical constituents of lemon balm (Melissa officinalis L.) cultivated in three different hydroponic systems. Industrial Crops and Products 163, 113313. https://doi.org/10.1016/j.indcrop.2021.113313
Sorensen JM. 2000. Melissa officinalis. International Journal of Aromatherapy 10(1), 7-15. https://doi.org/10.1016/S0962-4562(00)80004-1
Taiz L, Zeiger E, Møller IM, Murphy A. 2015. Plant physiology, 6th ed. Sinauer Associates Inc., Massachusetts, 761 p. Viršilė A, Olle M, Duchovskis P. 2017. LED lighting in horticulture. In: Dutta Gupta S. (eds) Light Emitting Diodes for Agriculture. Springer, Singapore. https://doi.org/10.1007/978-981-10-5807-3_7
Wacker A, Piepho M, Harwood JL, Guschina IA, Arts MT. 2016. Light-induced changes in fatty acid profiles of specific lipid classes in several freshwater Phytoplankton species. Frontiers in Plant Science 7, 264. https://doi.org/10.3389/fpls.2016.00264
Wang H, Gu M, Cui J, Shi K, Zhou Y, Yu J. 2009. Effects of light quality on CO2 assimilation, chlorophyllfluorescence quenching, expression of Calvin cycle genes and carbohydrate accumulation in Cucumis sativus. Journal of Photochemistry and Photobiology B: Biology 96(1), 30-37. https://doi.org/10.1016/j.jphotobiol.2009.03.010
Warrington IJ, Mitchell KJ. 1976. The influence of blueand red-biased light spectra on the growth and development of plants. Agricultural Meteorology 16, 247-262. https://doi.org/10.1016/0002-1571(76)90045-5
Wojciechowska R, Długosz-Grochowska O, Kołton A, Żupnik M. 2015. Effects of LED supplemental lighting on yield and some quality parameters of lamb’s lettuce grown in two winter cycles. Scientia Horticulturae 187, 80-86. https://doi.org/10.1016/j.scienta.2015.03.006
Wu B, Hitti Y, MacPherson S, Orsat V, Lefsrud MG. 2020. Comparison and perspective of conventional and LED lighting for photobiology and industry applications. Environmental and Experimental Botany 171, 103953. https://doi.org/10.1016/j.envexpbot.2019.103953
Wu MC, Hou CY, Jiang CM, Wang YT, Wang CY, Chen HH, Chang HM. 2007. A novel approach of LED light radiation improves the antioxidant activity of pea seedlings. Food Chemistry 101(4), 1753-1758. https://doi.org/10.1016/j.foodchem.2006.02.010
Xu F, Cao S, Shi L, Chen W, Su X, Yang Z. 2014. Blue light irradiation affects anthocyanin content and enzyme activities involved in postharvest strawberry fruit.Journal of Agricultural and Food Chemistry 62, 4778– 4783. https://doi.org/10.1021/jf501120u
Yanagi T, Okamoto K. 1997. Utilization of super-bright light emitting diodes as an artificial light source for plant growth. Acta Horticulturae 418, 223–228. https://doi.org/10.17660/ActaHortic.1997.418.30
Yi ZL, Huang WF, Ren Y, Onac E, Zhou GF, Peng S, Wang XJ, Li HH. 2014. LED lights increase bioactive substances at low energy costs in culturing fruiting bodies of Cordyceps militaris. Scientia Horticulturae 175, 139-143. https://doi.org/10.1016/j.scienta.2014.06.006
Yorio NC, Goins GD, Kagie HR, Wheeler RM, Sager JC. 2001. Improving spinach, radish, and lettuce growth under red light-emitting diodes (LEDs) with blue light supplementation. HortScience 36(2), 380-383. https://doi.org/10.21273/HORTSCI.36.2.380
Zahedi SM, Sarikhani H. 2017. The effect of end of day far-red light on regulating flowering of short-day strawberry (Fragaria × ananassa Duch. сv. Paros) in a long-day situation. Russian Journal of Plant Physiology 64, 83-90. https://doi.org/10.1134/S1021443717010198
 
International Journal of Horticultural Science and Technology
Volume 10, Special issue (Light in horticulture)
Special issue :Impact of light on crop production and quality
December 2023
Pages 66-88

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  • Receive Date: 18 January 2023
  • Revise Date: 01 March 2023
  • Accept Date: 10 May 2023

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