Omid Sohrabi; Abdollah Hatamzadeh; Azim Ghasemnezhad; Habibollah Samizadeh; Vahid Erfani-moghadam
Abstract
Medicinal plants have been used historically for various treatments and in human nutrition. Due to their natural occurrence and biodegradability, medicinal plants can be an eco-friendly alternative to toxic chemicals such as pesticides, fungicides, and herbicides. An in-vitro experiment was conducted ...
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Medicinal plants have been used historically for various treatments and in human nutrition. Due to their natural occurrence and biodegradability, medicinal plants can be an eco-friendly alternative to toxic chemicals such as pesticides, fungicides, and herbicides. An in-vitro experiment was conducted to test the impact of six medicinal plant extracts on tomato growth and callus induction. Two concentrations of each extract (50 and 100 mg L-1) were added to the Murashige and Skoog (MS) medium culture. Results showed that Juniperus sabina extract (50 mg L-1) accelerated tomato seed germination by 100% in vitro, compared to the control group in MS basal medium. Callus growth (CG) and callus weight (CW) increased by 50% and by more than 200% when using Taraxacum officinale (100 mg L-1) and Conocarpus erectus (50 mg L-1) extracts, respectively. Biochemical analysis revealed that the extracts were rich in phenolic compounds (348 mg g-1 of total phenol), flavonoids (162 mg g-1), antioxidants (61%), and auxin. Antioxidant activity, plant growth regulators (PGR), and plant defense elicitation probably contributed to these outcomes. Plant extracts also affected the biochemical content of calluses, except total phenol. We recommend using these plant extracts to increase growth, accelerate seed germination, and promote callus induction. Optimal concentrations and combinations of medicinal plant extracts for maximum benefit to different plant species and their growth-related values require further research.
Dabit Bista; Dikshya Sapkota; Hemanta Paudel; Gaurav Adhikari
Abstract
A field experiment was conducted at Horticulture Farm of Agriculture and Forestry University, Rampur, Chitwan, Nepal from 1 December to30 April of 2018/19 to evaluate the effect of plant growth regulators on onion (Allium cepa cv. Nasik-53). The experiment was laid out in a Randomized Complete Block ...
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A field experiment was conducted at Horticulture Farm of Agriculture and Forestry University, Rampur, Chitwan, Nepal from 1 December to30 April of 2018/19 to evaluate the effect of plant growth regulators on onion (Allium cepa cv. Nasik-53). The experiment was laid out in a Randomized Complete Block Design (RCBD) with 13 treatments. Growth regulators including: GA3 and NAA each at 75, 150 and 200 mg L-1 concentrations together with the combined forms were applied at 3 and 7 leaf stages of onion crop and the obtained data were compared with the control (distilled water spray) plants. Each treatment was replicated three times. The application of combination of 150 mg L-1 NAA at 3 leaf stage and 150 mg L-1 GA3 at 7 leaf stage resulted in highest values for plant height (76.67 cm), number of leaves (11.33), stem diameter (2.19 cm), bulb diameter (7.55 cm) and fresh weight (72.66 gm) while the control treatment resulted in the lowest values for all these attributes. Therefore, the combined application of 150 mg L-1 of NAA at 3-leaf stage and 150 mg L-1 of GA3 at 7 leaf stage can be recommended to enhance the growth and yield of onion.
Naser Askari; Richard G.F. Visser; Geert-Jan De Klerk
Abstract
In micropropagation of lily, preferably bulblets should be produced: Because bulblets are compact and robust, they are much easier to handle and to plant in soil than shoots. In this review, the various factors that determine bulblet growth in vitro are discussed. Gibberellins, jasmonates (JA) and abscisic ...
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In micropropagation of lily, preferably bulblets should be produced: Because bulblets are compact and robust, they are much easier to handle and to plant in soil than shoots. In this review, the various factors that determine bulblet growth in vitro are discussed. Gibberellins, jasmonates (JA) and abscisic acid (ABA) are the major identified plant growth regulators (PGRs) for storage organ formation. They also play a major role in lily bulblet growth in vitro. Growth conditions such as temperature and light (quantity and quality) strongly affect lily bulblet growth in tissue culture. Moderate abiotic stresses are introduced as new tool to improve storage organ formation in vitro. The amounts of endogenous carbohydrates (starch) in the explant and exogenous carbohydrates in the medium (sucrose) influence bulblet growth in vitro. It is also discussed how compounds present in the medium or in the scale-explants are translocated to the regenerating bulblet.
