Growth of Lily Bulblets In Vitro, a Review

Document Type: Research paper

Authors

1 Department of Plant Sciences, University of Jiroft, Jiroft, Iran. Plant Breeding, Wageningen University & Research, Wageningen,The Netherlands.

2 Plant Breeding, Wageningen University & Research, Wageningen,The Netherlands.

Abstract

In micropropagation of lily, preferably bulblets should be produced: Because bulblets are compact and robust, they are much easier to handle and to plant in soil than shoots. In this review, the various factors that determine bulblet growth in vitro are discussed. Gibberellins, jasmonates (JA) and abscisic acid (ABA) are the major identified plant growth regulators (PGRs) for storage organ formation. They also play a major role in lily bulblet growth in vitro. Growth conditions such as temperature and light (quantity and quality) strongly affect lily bulblet growth in tissue culture. Moderate abiotic stresses are introduced as new tool to improve storage organ formation in vitro. The amounts of endogenous carbohydrates (starch) in the explant and exogenous carbohydrates in the medium (sucrose) influence bulblet growth in vitro. It is also discussed how compounds present in the medium or in the scale-explants are translocated to the regenerating bulblet.

Keywords


Aksenova N.P, Konstantinova T.N, Sergeeva L.I, Macháčková I, Golyanovskaya S.A. 1994. Morphogenesis of potato plants in vitro. I. Effect of light quality and hormones. Journal of Plant Growth Regulation 13, 143-146.

Askari N. 2016. Aspects of bulblet growth of lily in vitro. Wageningen University PhD Thesis.130.  DOI: 10.18174/375959

Askari N, De Klerk G.J. 2012. Avoidance of cross-contamination during the  initiation step in lily tissue culture. The Lily Yearbook North American Lily Society 39-44.

Askari N, De Klerk G.J. 2018. The effect of light quality on lily growth in vitro. 2th International and 3rd National Ornamental Plant Congress. Iran.

Askari N, Visser R, De Klerk G.J. 2016. The role of abiotic stresses on lily bulblet growth in vitro. Propagation of Ornamental Plants 16, 130-136.

Askari N, Wang Y.G, De Klerk G.J. 2014. In tissue culture of Lilium explants may become heavily contaminated by the standard initiation procedure. Propagation of Ornamental Plants 14, 49-56.

Bach A, Świderski A. 2000. The effect of light quality on organogenesis of Hyacinthus orientalis L. in vitro. Acta Biologica Cracoviensia. Series Botanica 42, 115-120.

Bahr L.R, Compton M.E. 2004. Competence for in vitro bulblet regeneration among eight Lilium genotypes. HortScience 39, 127-129.

Beattie D, White J. 1993. Lilium-hybrids and species. In: The Physiology of Flower Bulbs. A. De Hertogh and M. Le Nard (eds) Elsevier Science Publishers.

Bonnier F, Van Tuyl J. 1997. Long term in vitro storage of lily: effects of temperature and concentration of nutrients and sucrose. Plant Cell, Tissue and Organ Culture 49, 81-87.

Chopra J, Kaur N, Gupta A.K. 2007. Sustained activities of carbon metabolizing enzymes determine seed size in Vigna radiata (mungbean). Current Science-Bangalore 92, 1420-1424.

De Jong P. 1974. Some notes on the evolution of lilies. The Lily Yearbook of the North American Lily Society.

De Klerk G.J, Kim K, Van Schadewijk M, Gerrits M. 1992. Growth of bulblets of Lilium speciosum in vitro and in soil. Acta Horticulturae 325, 513-520.

Desjardins Y, Dubuc J, Badr A. 2009. In vitro culture of plants: a stressful activity. Acta Horticulturae  812, 29-50.

George E.F, Hall M.A, De Klerk G.J. 2008. Plant Propagation by Tissue Culture: Volume 1. The Background. Springer 520.

Han B.H, Yae B.W, Yu H.J, Peak K.Y. 2005. Improvement of in vitro micropropagation of Lilium oriental hybrid ‘Casa blanca’by the formation of shoots with abnormally swollen basal plates. Scientia Horticulturae 103, 351-359.

Ilczuk A, Winkelmann T, Richartz S, Witomska M, Serek M. 2005. In vitro propagation of Hippeastrum × chmielii Chm. influence of flurprimidol and the culture in solid or liquid medium and in temporary immersion systems. Plant Cell, Tissue and Organ Culture 83, 339-346.

Ishimori T, Niimi Y, Han D.S. 2007. Benzyladenine and low temperature promote phase transition from juvenile to vegetative adult in bulblets of Lilium× formolongi ‘White Aga’cultured in vitro. Plant Cell,Tissue and Organ Culture 88, 313-318.

Jásik J, De Klerk G.J. 2006. Effect of methyl jasmonate on morphology and dormancy development in lily bulblets regenerated in vitro. Journal of Plant Growth Regulation 25, 45-51.

Ji Q, Vincken J.P, Suurs L.C, Visser R.G. 2003. Microbial starch-binding domains as a tool for targeting proteins to granules during starch biosynthesis. Plant Molecular Biology 51, 789-801.

Kato T, Shinmura D, Taniguchi A. 2007. Activities of enzymes for sucrose-starch conversion in developing endosperm of rice and their association with grain filling in extra-heavy panicle types. Plant Production Science 10, 442-450.

Keller E. 1993. Sucrose, cytokinin, and ethylene influence formation of in vitro bulblets in onion and leek. Genetic Resources and Crop Evolution 40, 113-120.

Kim K.S, Davelaar E, De Klerk G.J. 1994. Abscisic acid controls dormancy development and bulb formation in lily plantlets regenerated in vitro. Physiologia Plantarum 90, 59-64.

Knypl J. 1980. Stimulation of bulb growth in onion (Allium cepa L.) by N, N-diethyl-N-(2-hydroxyethyl) glycine. Biologia Plantarum 22, 226-230.

Kuijpers A.M, Langens-Gerrits M.M. 1996. Propagation of tulip in vitro. Acta Horticulturae 430, 321-324.

Kumar S, Kanwar J, Sharma D. 2006. In vitro propagation of Lilium. Advances in Horticultural Science 2, 181-188.

Kumar S, Kashyap M, Sharma D. 2005. In vitro regeneration and bulblet growth from lily bulb scale explants as affected by retardants, sucrose and irradiance. Biologia Plantarum 49, 629-632.

Langens-Gerrits M.M, De Klerk G.J. 1999. Micropropagation of flower bulbs. In: Plant Cell Culture Protocols, Springer, pp. 141-147.

Langens‐Gerrits M.M, De Klerk G.J, Croes A. 2003a. Phase change in lily bulblets regenerated in vitro. Physiologia Plantarum 119, 590-597.

Langens-Gerrits M.M, Kuijpers A.M, De Klerk G.J, Croes A. 2003b. Contribution of explant carbohydrate reserves and sucrose in the medium to bulb growth of lily regenerated on scale segments in vitro. Physiologia Plantarum 117, 245-255.

Langens-Gerrits M.M, Lilien-Kipnis H, Croes T, Miller W.B, Kollöffel C, De Klerk G.J. (1996). Bulb growth in lily regenerated in vitro. Acta Horticulturae 430, 267-274.

Le Guen‐Le Saos F, Hourmant A, Esnault F, Chauvin J. 2002. In vitro bulb development in shallot (Allium cepa L. Aggregatum Group): effects of anti‐gibberellins, sucrose and light. Annals of Botany 89, 419-425.

Leshem B, Lilien-Kipnis H, Steinitz B. 1982. The effect of light and of explant orientation on the regeneration and subsequent growth of bulblets on Lilium longiflorum Thunb, bulb-scale sections cultured in vitro. Scientia Horticulturae 17, 129-136.

Matsuo E, Arisumi K. 1978. Studies on the leaf development of the scale bulblet in the Easter lily (Lilium longiflorum). III. The relationship between the polarity of a scale and the leaf emergence of the scale bulblet or the plant type. Journal of the Japanese Society for Horticultural Science 47(3): 415-420

Matsuo T, Mizuno T. 1974. Changes in the amounts of two kinds of reserve glucose-containing polysaccharides during germination of the Easter lily bulb. Plant and Cell Physiology 15, 555-558.

Mohapatra P, Sarkar R, Kuanar S. 2009. Starch synthesizing enzymes and sink strength of grains of contrasting rice cultivars. Plant Science 176, 256-263.

Murashige T, Skoog F. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum 15, 473-497.

Niimi Y. 1995. In vitro propagation and post-in vitro establishment of bulblets of Lilium japonicum Thunb. Journal of the Japanese Society for Horticultural Science 63, 843-852.

Puijalon S, Piola F, Bornette G. 2008. Abiotic stresses increase plant regeneration ability. Evolutionary Ecology 22, 493-506.

Pumisutapon P, Topoonyanont N. 2017. Moderate-abiotic stress increase in vitro tuberization and microtuber growth of potato. Acta Horticulturae 1155.

Pumisutapon P, Visser R.G, De Klerk G.J. 2012. Moderate abiotic stresses increase rhizome growth and outgrowth of axillary buds in Alstroemeria cultured in vitro. Plant Cell, Tissue and Organ Culture 110, 395-400.

Ravnikar M, Žel J, Plaper I, Špacapan A. 1993. Jasmonic acid stimulates shoot and bulb formation of garlic in vitro. Journal of Plant Growth Regulation 12, 73-77.

Rice R, Alderson P, Wright N. 1983. Induction of bulbing of tulip shoots in vitro. Scientia Horticulturae 20, 377-390.

Saniewski M, Puchalski J. 1987. The effect of methyl jasmonate and abscisic acid on differentiation of benzyladenine-induced bulblets in Muscari bulbs. Biologia Plantarum 29, 63-65.

Santos I, Salema R. 2000. Promotion by Jasmonic acid of bulb formation in shoot cultures of Narcissus triandrus L. Plant Growth Regulation 30, 133-138.

Staikidou I, Watson S, Harvey B.M, Selby C. 2005. Narcissus bulblet formation in vitro: effects of carbohydrate type and osmolarity of the culture medium. Plant Cell, Tissue and Organ Culture 80, 313-320.

Taiz L, Zeiger E. 2002. Plant Physiology. 3rd edition. New York, Sinauer, 690.

Takayama S, Amo T, Fukano M. 1991. Rapid clonal propagation of Hyacinthus orientalis bulbs by shake culture. Scientia Horticulturae 45, 315-321.

Takayama S, Misawa M. 1982. Regulation of organ formation by cytokinin and auxin in Lilium bulbscales grown in vitro. Plant and Cell Physiology 23, 67-74.

Tanaka A, Hoshi Y, Kondo K, Taniguchi K. 1991. Induction and rapid propagation of shoot primordia from shoot apices of Lilium japonicum. Plant Tissue Culture Letters 8, 206-208.

Thakur R, Sood A. 2006. An efficient method for explant sterilization for reduced contamination. Plant Cell, Tissue and Organ Culture 84, 369-371.

Van Aartrijk J, Van der Linde P. 1986. In vitro propagation of flower-bulb crops. In: Tissue culture as a plant production system for horticultural crops, Springer 317-331.

Van Bel A. 1993. Strategies of phloem loading. Annual Review of Plant Biology 44, 253-281.

Van der Linde P, Schipper J. 1992. Micropropagation of iris with special reference to Iris x hollandica Tub. In: Biotechnology in Agriculture and Forestry 20, 173-197.

Viola R, Pelloux J, Van Der Ploeg A, Gillespie T, Marquis N, Roberts A.G, Hancock R.D. 2007. Symplastic connection is required for bud outgrowth following dormancy in potato (Solanum tuberosum L.) tubers. Plant, Cell & Environment 30, 973-983.

Vreugdenhil D, Sergeeva L.I. 1999. Gibberellins and tuberization in potato. Potato Research 42, 471-481.

Vreugdenhil D, Bindels P, Reinhoud P, Klocek J, Hendriks T. 1994. Use of the growth retardant tetcyclacis for potato tuber formation in vitro. Plant Growth Regulation 14, 257-265.

Wang Q.M, Zhang L.M, Guan Y.A, Wang Z.L. 2006. Endogenous hormone concentration in developing tuberous roots of different sweet potato genotypes. Agricultural Sciences in China 5, 919-927.

Xu X, van Lammeren A.A, Vermeer E, Vreugdenhil D. 1998. The role of gibberellin, abscisic acid, and sucrose in the regulation of potato tuber formation in vitro. Plant Physiology 117, 575-584.

Zhang Y, Zhang Q, Xue X. 2010. The effects of the photoperiods on the bulblet formation and sugar metabolism change of wild Lilium lancifoiium in vitro. Acta Horticulturae Sinica 201, 957-962.

Zimmermann M.H, Ziegler H. 1975. List of sugars and sugar alcohols in sieve-tube exudates. Encyclopedia of Plant Physiology. Springer, New York 480-503.