Ali Tanhaei; Ahmad Reza Dadras; Hossein Sabouri; Ebrahim Gholamalipour Alamdari; Sayed Javad Sajadi; Hossein Hosseini Moghaddam
Abstract
Olive (Olea europaea L.) is a historic and significant Mediterranean tree that has been widely used for its curative properties and oily nature. Images of 150 randomly selected fruits were captured and processed in three replications to investigate the genetic diversity among 98 olive genotypes. The ...
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Olive (Olea europaea L.) is a historic and significant Mediterranean tree that has been widely used for its curative properties and oily nature. Images of 150 randomly selected fruits were captured and processed in three replications to investigate the genetic diversity among 98 olive genotypes. The difference in all traits between genotypes was significant (P˂0.01), indicating a high level of genetic diversity among the olive genotypes. D1 outperformed other genotypes in terms of fruit area, major axis length, convex area, filled area, and equiv. diameter. The major axis length of the fruit exhibited a significant positive correlation with the perimeter, equivalent diameter, major axis length, and area of the stone (P≤0.01). Also, there was a significant, positive correlation between the minor axis lengths of the fruit and stone. The explained percentage of the traits’ associated markers indicated that the fruits’ major axis length had the highest cumulative coefficient (39%) with five bands. The IJS9-A and ScoT21-B genes regulated the most significant number of traits. The former regulated seven characteristics, i.e. fruit area, major axis length, minor axis length, convex area, filled area, equivalent diameter, and perimeter. In comparison, the latter regulated six characteristics, i.e. fruit area, major axis length, convex area, filled area, and the equivalent diameter of the fruit. Cluster analysis was used for categorizing genotypes into two groups. The findings of this study can be applied in hybridization and production programs for developing genotypes with more suitable fruits.
Atefeh Gholizadegan; َAlireza Seifi
Abstract
Fusarium wilt is one of the most destructive diseases of muskmelon (Cucumis melo L.), which is an economically important disease worldwide causes yield losses in muskmelon growing areas. One of the most effective controlling measures to prevent Fusarium wilt is through host resistance by using resistance ...
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Fusarium wilt is one of the most destructive diseases of muskmelon (Cucumis melo L.), which is an economically important disease worldwide causes yield losses in muskmelon growing areas. One of the most effective controlling measures to prevent Fusarium wilt is through host resistance by using resistance genes. We used developed molecular markers for Fom-2 gene, which confers resistance to race 1 of Fusarium in muskmelon, to screen muskmelon landraces in Khorasane-e-Razavi, Iran. After validation of the markers on a differential set of resistant and susceptible lines, we identified STS312 marker as the polymorphic and easy-to-score marker. Then we used STS312 to genotype plants from five different landraces. Our results suggest that resistance allele of Fom-2 gene is present in two landraces: Eyvankey and Mashhadi. These landraces can be used by muskmelon breeders to enhance resistance to Fusarium wilt in muskmelon.
Mansoureh Danesh; Mahmoud Lotfi; Shiva Azizinia
Abstract
Iran is a part of the secondary centre of origin of melons, and therefore, there is a wealth of genetic variation of these species in this country. The Amplified Fragment Length Polymorphism (AFLP) marker was applied to investigate the genetic variations among five major Iranian melon cultigens (Cucumis ...
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Iran is a part of the secondary centre of origin of melons, and therefore, there is a wealth of genetic variation of these species in this country. The Amplified Fragment Length Polymorphism (AFLP) marker was applied to investigate the genetic variations among five major Iranian melon cultigens (Cucumis melo) and ‘Ananasi’ as a general well-known cultivar. Ten primer pairs were used on 90 individuals producing 318 polymorphic fragments, with an average of 31.8 fragments per primer combination. The polymorphism rates ranged from 80 to 100%. The genetic similarities among accessions were calculated according to Dice’s Similarity Index and used to construct a dendrogram based on the Unweighted Pair Group Method with Arithmetic Average (UPGMA). The genetic distance estimates based on AFLPs ranged from 0.29 to 0.63, with a mean of 47±0.3. Iranian melon genotypes and the ‘Ananasi’ cultivar were considered as two separate groups on the cluster analysis. The principal coordinate analysis showed a separate allocation of the melon cultivar groups. The results demonstrated a wide diversity of Iranian melon cultigens. The high number of alleles and the high expected genetic diversity detected with the AFLP marker indicated that the Iranian melon cultigens had distinctive characteristics and were an important genetic diversity pool, which made them a valuable source of breeding materials.