Document Type: Research paper

Authors

Department of Biotechnology and Plant Breeding, Faculty of Agriculture, Ferdowsi University of Mashhad

10.22059/ijhst.2020.300685.359

Abstract

Fusarium wilt is one of the most destructive diseases of muskmelon (Cucumis melo L.), which is an economically important disease worldwide causes yield losses in muskmelon growing areas. One of the most effective controlling measures to prevent Fusarium wilt is through host resistance by using resistance genes. We used developed molecular markers for Fom-2 gene, which confers resistance to race 1 of Fusarium in muskmelon, to screen muskmelon landraces in Khorasane-e-Razavi, Iran. After validation of the markers on a differential set of resistant and susceptible lines, we identified STS312 marker as the polymorphic and easy-to-score marker. Then we used STS312 to genotype plants from five different landraces. Our results suggest that resistance allele of Fom-2 gene is present in two landraces: Eyvankey and Mashhadi. These landraces can be used by muskmelon breeders to enhance resistance to Fusarium wilt in muskmelon.

Keywords

  1. Banihashemi Z. 2010. Reaction of Cucumis melo cultivars to races of Fusarium oxysporum f. sp. melonis the cause of melon vascular wilt. Iranian Journal of Plant Pathology46 (1), 11-22.
  2. Banihashemi Z, Dezeeuw D. 1973. The effect of soil temperature on survival of Fusarium oxysporum f. melonis (Leach and Currence) Snyder and Hansen. Plant and Soil38(2), 465-468.
  3. Belavadi V. 2019. Floral biology and pollination in Cucumis melo L., a tropical andromonoecious cucurbit. Journal of Asia-Pacific Entomology22(1), 215-225.
  4. Brotman Y, Normantovich M, Goldenberg Z, Zvirin Z, Kovalski I, Stovbun N, Doniger T, Bolger A.M, Troadec C, Bendahmane A, Cohen R. 2013. Dual resistance of melon to Fusarium oxysporum races 0 and 2 and to Papaya ring-spot virus is controlled by a pair of head-to-head-oriented NB-LRR genes of unusual architecture. Molecular Plant 6(1), 235-238.
  5. Chikh-Rouhou H, González-Torres R, Alvarez J.M, Oumouloud A. 2010. Screening and morphological characterization of melons for resistance to Fusarium oxysporum f. sp. melonis race 1.2. HortScience45(7), 1021-1025.
  6. Ellis J, Dodds P, Pryor T. 2000. Structure, function and evolution of plant disease resistance genes. Current Opinion in Plant Biology 3(4), 278-284.
  7. Fulton T. M, Chunwongse J, Tanksley S.D. 1995. Microprep protocol for extraction of DNA from tomato and other herbaceous plants. Plant Molecular Biology Reporter 13(3), 207-209.
  8. Jones J.D, Dangl J.L. 2006. The plant immune system. Nature 444(7117), 323-329.
  9. Joobeur T, King J.J, Nolin S.J, Thomas, C.E, Dean R.A. 2004. The fusarium wilt resistance locus Fom-2 of melon contains a single resistance gene with complex features. The Plant Journal 39(3), 283-297.

10. Madadkhah E, Lotfi M, Nabipour A, Rahmanpour S, Banihashemi Z, Shoorooei M. 2012. Enzymatic activities in roots of melon genotypes infected with Fusarium oxysporum f. sp. melonis race 1. Scientia Horticulturae 135, 171-176.

11. Maleki M, Shojaeiyan A, Monfared S.R. 2018. Population structure, morphological and genetic diversity within and among melon (Cucumis melo L.) landraces in Iran. Journal of Genetic Engineering and Biotechnology 16(2), 599-606.

12. Nations F.a.A.O.o.t.U. 2018. FAOSTAT Statistical Database.

13. Oumouloud A, El-Otmani M, Chikh-Rouhou H, Claver A.G, Torres R.G, Perl-Treves R, Alvarez J. 2013. Breeding melon for resistance to Fusarium wilt: recent developments. Euphytica 192(2), 155-169.

14. Perchepied L, Dogimont C, Pitrat M. 2005. Strain-specific and recessive QTLs involved in the control of partial resistance to Fusarium oxysporum f. sp. melonis race 1.2 in a recombinant inbred line population of melon. Theoretical and Applied Genetics 111(1), 65-74.

15. Raghami M, López-Sesé A.I, Hasandokht M.R, Zamani Z, Moghadam M.R.F, Kashi A. 2014. Genetic diversity among melon accessions from Iran and their relationships with melon germplasm of diverse origins using microsatellite markers. Plant Systematics and Evolution 300(1), 139-151.

16. Risser G, Banihashemi Z, Davis D. 1976. A proposed nomenclature of Fusarium oxysporum f. sp. melonis races and resistance genes in Cucumis melo. Phytopathology 66 (9),1105-1106.

17. Sousaraei N, Ramshini H, Lotfi M, Sharzei A. 2018. Marker assisted backcrossing for introgression of Fusarium wilt resistance gene into melon. Euphytica 214(1), 7. https://doi.org/10.1007/s10681-017-2080-4

18. Wang S, Yang J, Zhang M. 2011. Developments of functional markers for Fom-2-mediated fusarium wilt resistance based on single nucleotide polymorphism in melon (Cucumis melo L.). Molecular Breeding 27(3), 385-393.

19. Wang, Y.H, Thomas C.E,  Dean R.A. 2000. Genetic mapping of a fusarium wilt resistance gene (Fom-2) in melon (Cucumis melo L.). Molecular Breeding 6(4), 379-389.