Ali Pourkhaloee; Morteza Khosh-Khui; Paul Arens; Hassan Salehi; Hooman Razi; Ali Niazi; Alireza Afsharifar; Jaap van Tuyl
Abstract
The genus Tulipa L. (Liliaceae) comprises about 100 species and Iran is considered as one of the main origins of tulips. In this research, genetic diversity and population structure of 27 wild populations of tulips collected from Iran were studied by 15 highly polymorphic and reproducible expressed sequenced ...
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The genus Tulipa L. (Liliaceae) comprises about 100 species and Iran is considered as one of the main origins of tulips. In this research, genetic diversity and population structure of 27 wild populations of tulips collected from Iran were studied by 15 highly polymorphic and reproducible expressed sequenced tag-simple sequence repeat (EST-SSR) markers and 8 nucleotide binding site (NBS)-enzyme combinations. According to EST-SSR genotyping, the average of gene diversity (GD) and polymorphism information content (PIC) were 0.66 and 0.62, respectively. However, the values of GD and PIC were equal for each NBS primer–enzyme combination which ranged between 0.85-0.95 with a mean value of 0.91. The mean value of resolving power (EST-SSR = 1.93; NBS-LRR = 17.39) indicated that the NBS markers had higher discriminatory power compared to the EST-SSR markers. UPGMA clustering confirmed the results of PCA which was further confirmed by Bayesian model-based STRUCTURE analysis. Population structure analysis detected 3 and 4 gene pools for 27 wild tulip germplasms with EST-SSR genotyping and NBS-LRR profiling, respectively. The AMOVA results indicated that molecular variation among populations (ΦPT = inter-population variation) was 82% and 93% of the total variation for EST-SSR and NBS-LRR markers, respectively. The results of this study will help the conservation and phylogenetic studies of tulips.
Mehrnaz Zarei; Hassan Salehi; Abolfazl Jowkar
Abstract
Plant propagation originated from the mature tissues is the most desirable method in producing clonal plants, however microbial contamination usually is the main concern during regeneration processes. Moreover, commercial production of plants using clonal propagation is considered as high throughput ...
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Plant propagation originated from the mature tissues is the most desirable method in producing clonal plants, however microbial contamination usually is the main concern during regeneration processes. Moreover, commercial production of plants using clonal propagation is considered as high throughput method due to the removal of seasonal barriers, lower cost of production with higher yield when compared to the conventional propagation methods. In this research the regulatory roles of 2-iso-pentenyl adenine (2iP) and Zeatin (Z) along with two temperature regimes, 22 and 28 °C, on mature explants of Picea abies (L.) H. Karst was evaluated for adventitious shoot regeneration. Norway spruce micro-cuttings considerably benefited from Ridomil and Benomyl treatments for fungal decontamination in explants taken in spring or fall. The highest percentage of regenerated explants (68.75%) as well as the highest numbers of adventitious shoots per explant were obtained in MS media supplemented with 4 µM Z. Furthermore, the highest length of proliferated shoot (1.17 cm) was achieved at 3.2 µM 2iP treatment. However, there were no significant differences in the length of adventitious shoots using different concentrations of 2iP. Incubating explants at 28˚C resulted in null or deformed regeneration in Norway spruce.
Somayeh Esmaeili; Hassan Salehi; Morteza Khosh-Khui
Abstract
Landscape groundcover plants are a diverse group of trailing or spreading species that naturally form continuous soil coverage.Ground cover plants that were used in this study consisted of vegetative propagules of: Vinca minor L. ٬Variegatum’, Vinca minor L. ٬Green’, Oxalis brasiliensis ...
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Landscape groundcover plants are a diverse group of trailing or spreading species that naturally form continuous soil coverage.Ground cover plants that were used in this study consisted of vegetative propagules of: Vinca minor L. ٬Variegatum’, Vinca minor L. ٬Green’, Oxalis brasiliensis G. Lodd, Trifolium repens L., Phyla nodiflora L. and Frankenia thymifolia Desf. The aim of this study was to compare some physiological and biochemical responses of each species used under seasonal changes in Shiraz climatic conditions. Several factors including chlorophyll and relative water content (RWC), electrolyte leakage (EL), proline content, soluble sugars and starch levels and antioxidant enzyme activities of these ground covers were investigated. Results showed that chlorophyll and RWC content were decreased in all ground cover plants during summer time. Furthermore, considerable seasonal variations in antioxidant enzyme activities were detected in all of the studied plants. The highest activity of CAT was obtained in summer, while SOD, POD and APX had their highest activities in autumn and winter seasons. Proline concentration in F. thymifolia was higher than the other species, especially in mid-winter. According to the results of this research, it appears that plants show different mechanisms against natural adverse environmental conditions. It can be concluded that F. thymifolia and P. nodiflora are well-matched with warm season and T. repens and V. minor are well-matched with cool season environmental conditions. Therefore, these plants can be recommended for cultivation in Shiraz green space and the same conditions elsewhere.
