Document Type : Research paper

Authors

Department of Horticultural Science, School of Agriculture, Shiraz University, Shiraz, Iran.

Abstract

Landscape groundcover plants are a diverse group of trailing or spreading species that naturally form continuous soil coverage.Ground cover plants that were used in this study consisted of vegetative propagules of: Vinca minor L. ٬Variegatum, Vinca minor L. ٬Green, Oxalis brasiliensis G. Lodd, Trifolium repens L., Phyla nodiflora L. and Frankenia thymifolia Desf. The aim of this study was to compare some physiological and biochemical responses of each species used under seasonal changes in Shiraz climatic conditions. Several factors including chlorophyll and relative water content (RWC), electrolyte leakage (EL), proline content, soluble sugars and starch levels and antioxidant enzyme activities of these ground covers were investigated. Results showed that chlorophyll and RWC content were decreased in all ground cover plants during summer time. Furthermore, considerable seasonal variations in antioxidant enzyme activities were detected in all of the studied plants. The highest activity of CAT was obtained in summer, while SOD, POD and APX had their highest activities in autumn and winter seasons. Proline concentration in F. thymifolia was higher than the other species, especially in mid-winter. According to the results of this research, it appears that plants show different mechanisms against natural adverse environmental conditions. It can be concluded that F. thymifolia and P. nodiflora are well-matched with warm season and T. repens and V. minor are well-matched with cool season environmental conditions. Therefore, these plants can be recommended for cultivation in Shiraz green space and the same conditions elsewhere.

Keywords

Acar C, Var M. 2001. A study on the adaptations of some natural ground cover plants and on their implications in landscape architecture in the ecological conditions of trabzon. Turkish Journal of Agriculture and Forestry 25, 235-245.
Ashraf M, Foolad M.R. 2007. Roles of glycinebetaine and proline in improving plant abiotic stress resistance. Environmental and Experimental Botany 59, 206-216.
Baur-Hӧch B, Mächler F, Nӧsberger J. 1990. Effect of carbohydrate demand on the remobilization of starch in stolons and roots of white clover (Trifolium repens L.) after defoliation. Journal of Experimental Botany 41, 573-578.
Beauchamp C, Fridovich I. 1971. Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry 44, 276-287.
Britz S, Hungerford J, Lee D.R. 1985. Photoperiodic regulation of photosynthate partitioning leave of Digitaria dacumbens Stent. Plant Physio l78, 710-4.
Buysse J, Merckx R. 1993. An improved colorimetric method to quantify sugar content of plant tissue. Journal of Experimental Botany 44, 1627-1629.
Bӧller B, Nӧsberger J. 1983. Effects of temperature and photoperiod onstolon characteristics, dry matter partitioning, and nonstructuralcarbohydrate concentration of two white clover ecotypes. Crop Science23, 1057-1062.
Chance B, Maehly A.C. 1955. Assay of catalase and peroxidases. Methods in Enzymology. 2, 764-765.
Danckwerts J.E, Gordon A.J. 1989. Long-term partitioning, storage and remobilization of 14C assimilated by Trifolium repens (cv. Blanca). Annals of Botany 64, 533-544.
Dou J, Zhou S.U, Xu Z.F. 2004. Tropical native ground cover plants resources of southern Yunnan and their sustainable use in landscape. Journal of Zhejiang Forestry College 1, 54-61.
Dubois M, Gilles K.A, Hamilton J.K, Rebers, P.A, Smith F. 1956. Colorimetric method for determination of sugar and related substances. Analytical Chemistry 28, 350–356.
Easton C.L, Kleindorfer S. 2009. Effects of salinity levels and seed mass on germination in Australian species of Frankenia L. (Frankeniaceae). Environmental and Experimental Botany 65, 345-352.
Frankow -Lindberg B.E. 2001. Adaptation to winter stress in nine white clover populations:  changes in non-structural carbo-hydrates during exposure to simulated winter conditions and ‘Spring’ regrowth potential. Annals of Botany 88, 745-751.
Ghahreman A. 2000. Final report of plant cover study program and floristic of Kish Island. Kish Island, pp. 205.
Ghani A, Azizi M, Tehranifar A. 2010. Potential evaluating of five ornamental species of wild Achillea cultivated under Mashhad climate. Iran Horticultural Science Journal (Agriculture Science and Industry) 32, 25-31.
Gil R, Bautista I, Boscaiu M, Lidón A, Wankhade S, Sánchez H, Llinares J, Vicente O. 2014. Responses of five Mediterranean halophytes to seasonal changes in environmental conditions. AoB Plants 6.
Gusta L.V, Wilen R.W, Fu P. 1996. Low-temperature stress tolerance: The role of abscisic acid, sugars, and heat-stable proteins. HortScience 31, 39-46.
Hoekstra F.A, Golovina E.A, Buitink J. 2001. Mechanisms of plant desiccation tolerance. Trends in Plant Science 6, 431-438.
Huner N.P.A, Krol M, Williams J.P, Maissan E. 1988. Overwintering periwinkle (Vinca minor L.) exhibits increased. Plant Physiology 87, 721-726.
Khedr A.A, Abbas M.A, Abdel Wahid A.A, Quick W.P, Abogadallah G.M. 2003. Proline induces the expression of salt-stress-responsive proteins and may improve the adaptation of Pancratium maritimum L. to salt-stress. Journal of Experimental Botany 45, 2553-2562.
Kirtikar K.R, Basu B.D. 1975. In: Indian medicinal plants, 2nd ed. Jayeed Press, New Delhi 3, 1916 -1917.
Ksouri R, Megdiche W, Debez A, Falleh H, Grignon C, Abdelly C. 2007. Salinity effects on polyphenol content and antioxidant activities in leaves of the halophyte Cakile maritima. Plant Physiology and Biochemistry 45, 244-249.
Mächler F, Nӧsberger J. 1977. Effect of light intensity and temperature on apparent photosynthesis of altitudinal ecotypes of Trifolium repens L. Oecologia 31, 73-78.
Macolino S, Serena M, Leinauer B, Ziliotto U. 2010. Preliminary findings on the correlation between water soluble carbohydrate content in stolons and first year green-up of seeded bermudagrass cultivars. HortTechnology 20, 758-776.
McCready R.M, Guggolz J, Silviera V, Owens H.S. 1950. Determination of starch and amylase in vegetables. Analytical Chemistry 22, 1156–1158.
Montague T, Kjelgren R.K, Rupp L. 2000. Surface energy balance affects gas exchange and growth of two irrigated landscape tree species in an arid climate. Journal of the American Society for Horticultural Science 125, 299-309.
Nakano Y, Asada K. 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplast. Plant and Cell Physiology 22, 867-880.
Naz S, Ahmad S, Rasool S.A, Sayeed. S.A, Siddiqi R. 2006. Antibacterial activity directed isolation of compounds from Onosma hispidium. Microbial Resolution 161, 43-48.
Pittenger D.R, Shaw D.A, Hode D.R, Holt D.B. 2001. Responses of landscape ground covers to minimum irrigation. Journal of Environmental Horticulture 19, 78-84.
Ravikanth V, Ramesh P, Diwan P.V, Venkateswarlu Y. 2000. Halleridone and Hallerone from Phyla nodiflora (L.) Greene. Biochemical Systematic Ecology 28, 905-906.
Saadalla M.M, Shanahan J.F, Quick J.S. 1990. Heat tolerance in winter wheat: I. Hardening and genetic effects on membrane thermostability. Crop Science 30, 1243-1247.
Saini R.S, Sharme K.D, Dhankhar O.P, Kaushik R.A. 2001. Laboratory manual of analytical techniques in horticulture. India: Agrobios 49-50.
Sairam R.K, Rao K.V, Srivastava G.C. 2002. Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant Science 163, 1037-1046.
Samieiani E, Ansari H. 2014. Drought Stress Impact on Some Biochemical and Physiological Traits of 4 Ground covers (Lolium perenne, Potentilla spp, Trifolium repens and Frankinia spp) with Potential Landscape Usage. Journal of Periodontology 4, 53-60.
Schiavon M, Macolino S, Leinauer B, Ziliotto U. 2016. Seasonal changes in carbohydrate and protein content of seeded bermudagrasses and their effect on spring green-up. Journal of Agronomy and Crop Science 202, 151-160.
Shooshtarian S, Salehi H. 2011. Physiological and ecological investigation on adaption of some ground cover plants as turfgrass alternatives in arid landscape region of Kish Island during cool season. African Journal of Agricultural Research 7, 546-554.
Shooshtarian S, Tehranifar A. 2010. The study of application of xerophile ground cover plants in urban landscape of Mashhad. Journal of Mashhad Pazhoohi 1, 92-105. 
Terblanche F.C, Kornelius G. 1996. Essential oil constituents of the genus Lippia (Verbenaceae) – A literature review. Journal of Essential Oil Research8, 471-485.
Udomchalothorn T, Maneeprasobsuk S, Bangyeekhun E, Boon-Long P, Chadchawan S. 2009. The role of the bifunctional enzyme, fructose-6-phosphate-2-kinase/fructose-2, 6-bisphosphatase, in carbon partitioning during salt stress and salt tolerance in Rice (Oryza sativa L.). Plant Science 176, 334-341.
Van der Spuy U. 1976. Gardening with groundcovers. A.H. and A.W. Reed Wellington. pp. 112.
Vranova E, Inzé D, Van Breusegem F. 2002. Signal transduction during oxidative stress. Journal of Experimental Botany 53, 1227-1236.
Wachendorf M, Collins R.P, Elgersma A, Fothergill M, Frankow-Lindberg B.E, Ghesquiere A, Guckert A, Guinchard M.P, Helgadottir A, Lüscher A, Nolan T, Nykänen-Kurki P, Nösberger J, Parente G, Puzio S, Rhodes I, Robin C, Ryan A, Stäheli B, Stoffel S, Taube F, Connolly J. 2001. Overwintering and growing season dynamics of Trifolium repens L. in mixture with Lolium perenne L.: a model approach to plant–environment interactions. Annals of Botany 88, 683-702.
Weston L.A. 2002. Weed suppressive ground covers, a more attractive and effective way to manage weeds. Cornell University Turfgrass Times (CUTT) 12, 4-7.
Yang S.Q, Ren G.X, Yang G.H, Feng Y.Z, Zhang Q. 2007. Effects of Water stress on osmoregulation substances and chlorophyll fluorescent parameter for forage grass. Acta Botanica Boreal-occident Sinicia 27, 1826-1832.
Yang W.L, Liu J.M, Chen F, Liu Q, Gong Y.D, Zhao N.M. 2006. Identification of Festuca arundinacea Schreb. Cat1 Catalase Gene and analysis of its expression under abiotic stresses. Journal of Integrative Plant Biology 48, 334-340.