Ali Pourkhaloee; Morteza Khosh-Khui; Paul Arens; Hassan Salehi; Hooman Razi; Ali Niazi; Alireza Afsharifar; Jaap van Tuyl
Abstract
The genus Tulipa L. (Liliaceae) comprises about 100 species and Iran is considered as one of the main origins of tulips. In this research, genetic diversity and population structure of 27 wild populations of tulips collected from Iran were studied by 15 highly polymorphic and reproducible expressed sequenced ...
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The genus Tulipa L. (Liliaceae) comprises about 100 species and Iran is considered as one of the main origins of tulips. In this research, genetic diversity and population structure of 27 wild populations of tulips collected from Iran were studied by 15 highly polymorphic and reproducible expressed sequenced tag-simple sequence repeat (EST-SSR) markers and 8 nucleotide binding site (NBS)-enzyme combinations. According to EST-SSR genotyping, the average of gene diversity (GD) and polymorphism information content (PIC) were 0.66 and 0.62, respectively. However, the values of GD and PIC were equal for each NBS primer–enzyme combination which ranged between 0.85-0.95 with a mean value of 0.91. The mean value of resolving power (EST-SSR = 1.93; NBS-LRR = 17.39) indicated that the NBS markers had higher discriminatory power compared to the EST-SSR markers. UPGMA clustering confirmed the results of PCA which was further confirmed by Bayesian model-based STRUCTURE analysis. Population structure analysis detected 3 and 4 gene pools for 27 wild tulip germplasms with EST-SSR genotyping and NBS-LRR profiling, respectively. The AMOVA results indicated that molecular variation among populations (ΦPT = inter-population variation) was 82% and 93% of the total variation for EST-SSR and NBS-LRR markers, respectively. The results of this study will help the conservation and phylogenetic studies of tulips.
Somayeh Esmaeili; Hassan Salehi; Morteza Khosh-Khui
Abstract
Landscape groundcover plants are a diverse group of trailing or spreading species that naturally form continuous soil coverage.Ground cover plants that were used in this study consisted of vegetative propagules of: Vinca minor L. ٬Variegatum’, Vinca minor L. ٬Green’, Oxalis brasiliensis ...
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Landscape groundcover plants are a diverse group of trailing or spreading species that naturally form continuous soil coverage.Ground cover plants that were used in this study consisted of vegetative propagules of: Vinca minor L. ٬Variegatum’, Vinca minor L. ٬Green’, Oxalis brasiliensis G. Lodd, Trifolium repens L., Phyla nodiflora L. and Frankenia thymifolia Desf. The aim of this study was to compare some physiological and biochemical responses of each species used under seasonal changes in Shiraz climatic conditions. Several factors including chlorophyll and relative water content (RWC), electrolyte leakage (EL), proline content, soluble sugars and starch levels and antioxidant enzyme activities of these ground covers were investigated. Results showed that chlorophyll and RWC content were decreased in all ground cover plants during summer time. Furthermore, considerable seasonal variations in antioxidant enzyme activities were detected in all of the studied plants. The highest activity of CAT was obtained in summer, while SOD, POD and APX had their highest activities in autumn and winter seasons. Proline concentration in F. thymifolia was higher than the other species, especially in mid-winter. According to the results of this research, it appears that plants show different mechanisms against natural adverse environmental conditions. It can be concluded that F. thymifolia and P. nodiflora are well-matched with warm season and T. repens and V. minor are well-matched with cool season environmental conditions. Therefore, these plants can be recommended for cultivation in Shiraz green space and the same conditions elsewhere.
Nafiseh Geshnizjani; Morteza Khosh-khui
Abstract
Commercially available amino acids mixtures have several advantages such as enhancing assimilation of fertilizer, facilitating uptake of water and nutrients and improving photosynthesis of plants. To investigate the response of gerbera (Gerbera jamesonni L.) flowers ‘Saltino’ to foliar application ...
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Commercially available amino acids mixtures have several advantages such as enhancing assimilation of fertilizer, facilitating uptake of water and nutrients and improving photosynthesis of plants. To investigate the response of gerbera (Gerbera jamesonni L.) flowers ‘Saltino’ to foliar application of a mixture of 19 essential amino acids (0.25, 0.50 and 0.75 mg L-1) and ammonium nitrate (200 mg L-1) as nitrogen source, a pot experiment was carried out in the research greenhouse of Eram Botanical Garden of Shiraz University. Number of flowers, flower diameter, stems length, chlorophyll, proline, protein and nitrogen contents, photosynthesis rate, stomatal conductance, and vase life of flowers were significantly improved by amino acids treatments. No significant difference was observed between ammonium nitrate and amino acids mixture for flower fresh and dry weights. Results suggested that application of amino acids mixture can induce acetyl CoA, which plays an improvingrole in physiological processes in plants. Application of amino acids mixture as a promising and sustainable approach can be recommended to promote quality and quantity of gerbera flowers.
Zahra Afifipour; Morteza Khosh-Khui
Abstract
Tuberose (Polianthes tuberosa L.) is one of the most important cut flowers in Iran. This research was conducted to investigate the effects of spraying a mixture of amino acids on the physiological and morphological characteristics of tuberose. The experiment was carried out in two successive years (2009 ...
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Tuberose (Polianthes tuberosa L.) is one of the most important cut flowers in Iran. This research was conducted to investigate the effects of spraying a mixture of amino acids on the physiological and morphological characteristics of tuberose. The experiment was carried out in two successive years (2009 and 2010) using two tuberose cultivars ‘Dezfuli’ and ‘Mahallati’. The experiment was arranged as a factorial on a completely randomized design with 4 replications. Treatments were 0, 0.25, 0.5, and 0.75 mg L-1 of amino acids. The results showed that applying amino acids improved all growth and flowering characteristics of tuberose cultivars. Concentration of 0.75 mg L-1 amino acids enhanced the floral stem, inflorescence, and stem diameters. The fresh weight of floral stem increased about 22% as a result of spraying with 0.25 mg L-1 amino acid mixture. Total protein and nitrogen contents of the ‘Dezfuli’ cultivar showed about 60% enhancement when using a 0.5 mg L-1 mixture of amino acid. In general, the effects of amino acids on ‘Dezfuli’ were greater than the ‘Mahallati’ cultivar.
Ali Pourkhaloee; Morteza Khosh-Khui
Abstract
Two separate factorial experiments were conducted to study the effects of explant sources, plant growth regulators, sucrose concentrations, and light conditions on in vitro cormlet formation of freesia (Freesia ×hybrida Bailey 'Argenta'). Interestingly, it was observed that the pupae had lower ...
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Two separate factorial experiments were conducted to study the effects of explant sources, plant growth regulators, sucrose concentrations, and light conditions on in vitro cormlet formation of freesia (Freesia ×hybrida Bailey 'Argenta'). Interestingly, it was observed that the pupae had lower contamination levels compared to mother corms. Using 40% sodium hypochlorite solution for 40 min, contamination levels of pupae and mother corms reduced to 19.80 and 46.40%, respectively. Moreover, pupae showed the highest regeneration frequency. In the first experiment, 6.67 cormlets were directly produced per pupa (cold storage-produced corm) on Murashige and Skoog (MS) medium supplemented with 6 mg L-1 1-naphthaleneacetic acid (NAA), 1 mg L-1 6-benzylaminopurine (BA), and 60 g L-1 sucrose, when cultures were stored in the dark. In the second experiment, on average, 5.67 shoots were proliferated per pupa explant in the presence of 4 mg L-1 BA and 2 mg L-1 Kinetin (Kin). Subculturing these shoots on MS medium containing 3 mg L-1 BA and 0.5 mg L-1 NAA led to production of 3.67 cormlets per shoot. Finally, in vitro derived cormlets showed the highest percentage of rooting (77.80%), root number (8.33), and root length (2.13 cm) on MS medium containing 1 mg L-1 indole-3-butyric acid (IBA).
Mehdi Abdolmaleki; Morteza Khosh-Khui; Saeid Eshghi; Asghar Ramezanian
Abstract
Rosa hybrida L. is an important commercial cut flower. Salicylic acid (SA) and calcium chloride (CaCl2) act as endogenous signal molecules responsible for growth parameters in plants. The aim of this study was to evaluate the effects of preharvest SA and CaCl2 treatments in extending the vase life of ...
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Rosa hybrida L. is an important commercial cut flower. Salicylic acid (SA) and calcium chloride (CaCl2) act as endogenous signal molecules responsible for growth parameters in plants. The aim of this study was to evaluate the effects of preharvest SA and CaCl2 treatments in extending the vase life of cut rose flowers. Therefore, a factorial experiment based on completely randomized design with SA (0, 150, 300, and 450 mg L-1) and CaCl2 (0, 0.75, 1.5, and 2.25%) with 4 replicates and 2 samples (individual flowers) in each replicate, was conducted. Changes in growth, macronutrient concentration, chlorophyll content, leaf relative water content (LRWC), flower quality, vase life, and membrane stability index were investigated in R. hybrida cv. “Dolce Vita.” Exogenously applied SA and CaCl2 increased plant growth (such as shoots and flower buds). Foliar application of SA and CaCl2 also increased macronutrient concentration (N, K, Ca, and Mg), chlorophyll content, LRWC, flower quality, and vase life; however membrane stability index was decreased with increasing levels of SA and CaCl2. These results suggest that SA and CaCl2 could be used as potential growth promoters to improve postharvest life of roses. According to the results of this experiment, SA and CaCl2 as natural, cheap, safe, and biodegradable compounds are suitable alternatives for conventional chemical treatments in order to prolong vase life of cut rose flowers. Commercialization of these compounds for optimum formulations needs further experiments.
Nafiseh Geshnizjany; Asghar Ramezanian; Morteza Khosh-Khui
Abstract
The purpose of this study was to evaluate the effects of Nano-silver (NS), calcium chloride (CaCl2) and their combinations on Gerbera jamesonii ‘Carambole’ cut flowers. The experiment was conducted as a completely randomized design (CRD) with 10 treatments and four replications, with three ...
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The purpose of this study was to evaluate the effects of Nano-silver (NS), calcium chloride (CaCl2) and their combinations on Gerbera jamesonii ‘Carambole’ cut flowers. The experiment was conducted as a completely randomized design (CRD) with 10 treatments and four replications, with three flowers in each replication. Treatments consisted of 5 mg L-1 NS, 1% and 2% CaCl2, 5 mg L-1 NS+ 1% CaCl2, 5 mg L-1 NS + 2% CaCl2, 0.1% and 0.2% CaCl2, 5 mg L-1 NS + 0.1% CaCl2, 5 mg L-1 NS + 0.2% CaCl2 and a control. It was revealed that CaCl2 postharvest spray, NS in a vase solution, as well as their combinations could significantly increase the vase life of gerbera flowers. The longest postharvest life of treated flowers was obtained from 5 mg L-1 NS and 5 mg L-1 NS + 1% CaCl2 treatments. The highest solution uptake was observed in 5 mg L-1 NS, 5 mg L-1 NS + 2% CaCl2 and 5 mg L-1 NS +1% CaCl2 treatments. Furthermore, application of NS and CaCl2 resulted in a reduced loss of relative fresh weight. The application of CaCl2 alone was able to increase all mentioned traits; however, this increase was less than 5 mg L-1 NS, 5 mg L-1 NS + 1% CaCl2 and 5 mg L-1 NS + 2% CaCl2 treatments. Additionally, in vitro cultivation and microscopic counting of microorganisms showed that microorganism growth at the end of flowering stems had been largely restricted by using NS solely or in combination with CaCl2.
Morteza Khosh-Khui
Abstract
Scented rose species are very important in the genus Rosa due to their use in highly-prized essential oil production and as garden roses. The fragrance of the rose flower (attar) captured by extraction is one of the most valuable flavor and fragrance products. A comprehensive study of the papers published ...
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Scented rose species are very important in the genus Rosa due to their use in highly-prized essential oil production and as garden roses. The fragrance of the rose flower (attar) captured by extraction is one of the most valuable flavor and fragrance products. A comprehensive study of the papers published in the field of in vitro culture and biotechnology of these species, particularly Damask rose, was performed. Then, in separate sections, the articles related to media preparation for callus culture, shoot regeneration and multiplication, rooting, and cell suspension culture were reviewed. In this paper, the stages of the in vitro culture of scented roses, including establishment, multiplication, in vitro rooting, acclimatization, and genetic variability are also considered. Subsequently, investigations on methods of in vitro culture of scented roses by callus, pollen, anther, cell suspension, and protoplast culture are described. Finally, technological improvements for micropropagation, biotechnology. pharmaceutical, and other properties of the Damask rose and other species of scented roses are discussed. Prospects of in vitro culture and biotechnological techniques of scented rose species are also discussed. Among scented roses, the focus of this paper is on four species, namely Rosa damascena Mill., R. gallica Linn., R. centifolia Linn., and R. moschata Herrm., which are highly prized for oil production.
