Ali Pourkhaloee; Morteza Khosh-Khui; Paul Arens; Hassan Salehi; Hooman Razi; Ali Niazi; Alireza Afsharifar; Jaap van Tuyl
Abstract
The genus Tulipa L. (Liliaceae) comprises about 100 species and Iran is considered as one of the main origins of tulips. In this research, genetic diversity and population structure of 27 wild populations of tulips collected from Iran were studied by 15 highly polymorphic and reproducible expressed sequenced ...
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The genus Tulipa L. (Liliaceae) comprises about 100 species and Iran is considered as one of the main origins of tulips. In this research, genetic diversity and population structure of 27 wild populations of tulips collected from Iran were studied by 15 highly polymorphic and reproducible expressed sequenced tag-simple sequence repeat (EST-SSR) markers and 8 nucleotide binding site (NBS)-enzyme combinations. According to EST-SSR genotyping, the average of gene diversity (GD) and polymorphism information content (PIC) were 0.66 and 0.62, respectively. However, the values of GD and PIC were equal for each NBS primer–enzyme combination which ranged between 0.85-0.95 with a mean value of 0.91. The mean value of resolving power (EST-SSR = 1.93; NBS-LRR = 17.39) indicated that the NBS markers had higher discriminatory power compared to the EST-SSR markers. UPGMA clustering confirmed the results of PCA which was further confirmed by Bayesian model-based STRUCTURE analysis. Population structure analysis detected 3 and 4 gene pools for 27 wild tulip germplasms with EST-SSR genotyping and NBS-LRR profiling, respectively. The AMOVA results indicated that molecular variation among populations (ΦPT = inter-population variation) was 82% and 93% of the total variation for EST-SSR and NBS-LRR markers, respectively. The results of this study will help the conservation and phylogenetic studies of tulips.
Ali Pourkhaloee; Morteza Khosh-Khui
Abstract
Two separate factorial experiments were conducted to study the effects of explant sources, plant growth regulators, sucrose concentrations, and light conditions on in vitro cormlet formation of freesia (Freesia ×hybrida Bailey 'Argenta'). Interestingly, it was observed that the pupae had lower ...
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Two separate factorial experiments were conducted to study the effects of explant sources, plant growth regulators, sucrose concentrations, and light conditions on in vitro cormlet formation of freesia (Freesia ×hybrida Bailey 'Argenta'). Interestingly, it was observed that the pupae had lower contamination levels compared to mother corms. Using 40% sodium hypochlorite solution for 40 min, contamination levels of pupae and mother corms reduced to 19.80 and 46.40%, respectively. Moreover, pupae showed the highest regeneration frequency. In the first experiment, 6.67 cormlets were directly produced per pupa (cold storage-produced corm) on Murashige and Skoog (MS) medium supplemented with 6 mg L-1 1-naphthaleneacetic acid (NAA), 1 mg L-1 6-benzylaminopurine (BA), and 60 g L-1 sucrose, when cultures were stored in the dark. In the second experiment, on average, 5.67 shoots were proliferated per pupa explant in the presence of 4 mg L-1 BA and 2 mg L-1 Kinetin (Kin). Subculturing these shoots on MS medium containing 3 mg L-1 BA and 0.5 mg L-1 NAA led to production of 3.67 cormlets per shoot. Finally, in vitro derived cormlets showed the highest percentage of rooting (77.80%), root number (8.33), and root length (2.13 cm) on MS medium containing 1 mg L-1 indole-3-butyric acid (IBA).
