Document Type : Research paper

Authors

1 Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute (NSTRI), Karaj, P.O. Box 31485498, Iran.

2 Bu Ali Sina University of Hamadan, Faculty of Agriculture, Department of Horticulture, Assistant Professor, Fruit / Apple

Abstract

Freezing damage is a significant environmental challenge that limits both the geographic distribution and production of pomegranates in the world. The objective of this research was to search for useful correlations between freezing tolerance and soluble carbohydrate and proline content, as well as stomatal density in different parts of the leaves, to allow indirect selection of winter hardiness in pomegranate cultivars. Our results showed that freezing tolerance at the non-acclimated stage (August) was not strongly correlated with freezing tolerance during deep winter dormancy in January. Cold tolerance in summer was strongly correlated with leaf stomatal density; however, a moderate correlation was observed between cold tolerance in winter and leaf stomatal density. The results showed that 'Alak', the most cold-tolerant cultivar in summer and winter, had the highest leaf stomatal density. 'Agha Mohammad Ali' cultivar had the lowest leaf stomatal density and cold tolerance in summer; however, this cultivar showed considerable cold tolerance in winter. Moreover, with the except of 'Agha Mohammad Ali', stomatal density in summer was related to soluble carbohydrate concentration in the stems, which could justify the correlation between stomatal density and winter hardiness in pomegranate trees. It was found that LT50 values in January were negatively related to soluble carbohydrate concentration in stems. However, there was no statistical correlation between winter hardiness in January and proline content in the stems. These results suggest that soluble carbohydrates and stomatal density are suitable indices for predicting freezing tolerance of pomegranate cultivars throughout the year and growing season, respectively.

Keywords

Ahmad I, Wainwright S, Stewart G. 1981. The solute and water relations of Agrostis stolonifera ecotypes differing in their salt tolerance. New Phytologist 87(3), 615-629.
Ashraf M, Foolad MR. 2007. Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environmental and experimental botany 59(2), 206- 216.
Aslamarz AA, Vahdati K. 2010. Stomatal density and ion leakage as indicators of cold hardiness in walnut. Acta Horticulturae 861, 321-324.
Bates LS, Waldren RP, Teare I. 1973. Rapid determination of free proline for water-stress studies. Plant and soil 39(1), 205-207.
Bosabalidis AM, Kofidis G. 2002. Comparative effects of drought stress on leaf anatomy of two olive cultivars. Plant Science 163(2), 375-379.
Boussiba S, Rikin AE. 1975. The role of abscisic acid in cross-adaptation of tobacco. Plant Physiology 56, 337- 339.
Dörffling K, Dörffling H, Lesselich G, Luck E, Zimmermann C, Melz G, Jürgens H. 1997. Heritable improvement of frost tolerance in winter wheat by in vitro-selection of hydroxyproline-resistant proline overproducing mutants. Euphytica 93(1), 1-10.
Drogoudi P, Pantelidis G, Manganaris A. 2012. Morphological and physiological characteristics in pomegranate cultivars with different yields. Options Méditerranéennes 103, 67-69.
Falconer D, Mackay T. 1996. Introduction toquantitative genetics (4th ed.). Prentice Hall, Longman Press. Fischer M. 1992. Frostresistenzuntersuchungen an Malus-Artbastard-Unterlagen. Erwerbsobstbau 34, 194-197.
Gailing O. 2008. QTL analysis of leaf morphological characters in a Quercus robur full-sib family (Q. robur× Q. robur ssp. slavonica). Plant Biology 10(5), 624-634.
Ghasemi-Soloklui AA, Ershadi A, Fallahi E. 2012. Evaluation of cold hardiness in seven Iranian commercial pomegranate (Punica granatum L.) cultivars. HortScience 47(12), 1821-1825.
Ghasemi-Soloklui AA, Gharaghani A, Oraguzie N, Eshghi S, Vazifeshenas M. 2017. Chilling and heat requirements of 20 Iranian pomegranate cultivars and their correlations with geographical and climatic parameters, as well as tree and fruit characteristics. HortScience 52(4), 560-565.
Ghasemi-Soloklui AA, Gharaghani A, Oraguzie N, SaedMoucheshi A, Vazifeshenas M. 2019. Genetic diversity, heritability and inter-relationships of fruit quality and taste attributes among Iranian pomegranate (Punica granatum L.) cultivars using multivariate statistical analysis. Fruits 74(6), 303–318.
Guy CL. 1990. Cold acclimation and freezing stress tolerance: Role of protein metabolism. Annual review of plant biology 41(1), 187-223.
Hsu S, Hsu Y, Kao C. 2003. The effect of polyethylene glycol on proline accumulation in rice leaves. Biologia Plantarum 46(1), 73-78.
Hummer K, Fuchigami LH, Peters V, Bell N. 1995. Cold hardiness in Rubus. Fruit varieties journal 49, 52-58.
Kalberer SR, Leyva-Estrada N, Krebs SL, Arora R. 2007. Frost dehardening and rehardening of floral buds of deciduous azaleas are influenced by genotypic biogeography. Environmental and Experimental Botany 59(3), 264-275.
Levitt J, Scarth GW. 1936. Frost-hardening studies with living cells: I. Osmotic and bound water changes in relation to frost resistance and the seasonal cycle. Canadian Journal of Research 14(7), 267-284.
Li PH, Weise CJ. 1971. Increasing cold resistance of stem section of Cornus stonlonifera by artificial dehydration: a preliminary report. Cryobiology 8, 108- 111. Lindén L. 2002. Measuring cold hardiness in woody plants. PhD thesis, University of Helsinki, Finland.
Liu C, Li Y, Xu L, Chen Z, He N. 2019. Variation in leaf morphological, stomatal, and anatomical traits and their relationships in temperate and subtropical forests. Scientific reports 9(1), 1-8.
Mancuso S, Fiorino P. 2000. Differential thermal analysis, supercooling and cell viability in organs of Olea europaea at subzero temperatures. Advances in horticultural science 14, 23-27.
Meena K, Singh R, Pareek S, Kashyap P, Sheikh M, Mokashi A, Rokhade A. 2011. Evaluation of pomegranate (Punica granatum L.) genotypes for morphological and flowering characteristics under semi-arid climate. Acta Horticulturae 890, 233-238.
Meng LS, Yao SQ. 2015. Transcription co-activator Arabidopsis Angostifolia3 (AN3) regulates water-use efficiency and drought tolerance by modulating stomatal density and improving root architecture by the transrepression of YODA (YDA). Plant Biotechnology Journal 13, 893-902.
Mittelstädt H. 1965. Beiträge zur Züchtungsforschung beim Apfel. Der Züchter 35(7), 311-327.
Morin X, Améglio T, Ahas R, Kurz-Besson C, Lanta V, Lebourgeois F, Miglietta F, Chuine I. 2007. Variation in cold hardiness and carbohydrate concentration from dormancy induction to bud burst among provenances of three European oak species. Tree Physiology 27(6), 817-825.
Muradoğlu F, Gündoğdu M. 2011. Stomata size and frequency in some walnut (Juglans regia) cultivars. International Journal of Agriculture & Biology 13, 1011–1015.
Nasrabadi M, Ramezanian A, Eshghi S, KamgarHaghighi AA, Vazifeshenas MR, Valero D. 2019. Biochemical changes and winter hardiness in pomegranate (Punica granatum L.) trees grown under deficit irrigation. Scientia Horticulturae 251, 39-47.
Nassuth A, Rahman MA, Nguyen T, Ebadi A, Lee C. 2021. Leaves of more cold hardy grapes have a higher density of small, sunken stomata. Vitis 60(2), 63-67.
Pagter M, Jensen CR, Petersen KK, Liu F, Arora R. 2008. Changes in carbohydrates, ABA and bark proteins during seasonal cold acclimation and deacclimation in Hydrangea species differing in cold hardiness. Physiologia Plantarum 134(3), 473-485.
Palonen P. 1999. Relationship of seasonal changes in carbohydrates and cold hardiness in canes and buds of three red raspberry cultivars. Journal of the American Society for Horticultural Science 124(5), 507-513.
Palta JP, Li PH. 1979. Frost-hardiness in relation to leaf anatomy and natural distribution of several solanum species. Crop Science 19(5), 665-671.
Patton AJ, Cunningham SM, Volenec JJ, Reicher ZJ. 2007. Differences in freeze tolerance of zoysiagrasses: II. Carbohydrate and proline accumulation. Crop Science 47(5), 2170-2181.
Pérez López D, Moriana Elvira A, Mariño Barnechea J, Gijón López MDC. 2010. Water relation response to soil chilling of six olive (Olea europaea L.) cultivars with different frost resistance. Spanish Journal of Agricultural Research, 8, 780-789.
Reyes-Díaz M, Alberdi M, Piper F, Bravo LA, Corcuera LJ. 2005. Low temperature responses of Nothofagus dombeyi and Nothofagus nitida, two evergreen species from south central Chile. Tree physiology 25(11), 1389-1398.
Roselli G, Venora G. 990. Relationship between stomatal size and winter hardiness in the olive. Acta Horticulturae 286, 89-92.
Roselli G, Benelli G, Morelli D. 1989. Relationship between stomatal density and winter hardiness in olive (Olea europaea L.). Journal of Horticultural Science 64(2), 199-203.
Rowland LJ, Ogden EL, Ehlenfeldt MK, Vinyard B. 2005. Cold hardiness, deacclimation kinetics, and bud development among 12 diverse blueberry genotypes under field conditions. Journal of the American Society for Horticultural Science 130(4), 508-514.
Sagaram M, Lombardini L, Grauke L. 2007. Variation in leaf anatomy of pecan cultivars from three ecogeographic locations. Journal of the American Society for Horticultural Science 132(5), 592-596.
Sheidai M, Khandan M, Nasre-Esfahani S. 2005. Cytogenetical study of some Iranian pomegranate (Punica granatum L.) cultivars. Caryologia 58(2), 132- 139.
Sirooeinejad B, Zamani Z, Fatahi MR. 2020. Study of physiological and biochemical responses to freezing stress in pomegranate (Punica granatum L.) trees during acclimation and deaclimation cycle. The Journal of Horticultural Science and Biotechnology 95(3), 341- 355.
Soleimani A, Lessani H, Talaie A. 2002. Relationship between stomatal density and ionic leakage as indicators of cold hardiness in olive (Olea europaea L.). Acta Horticulturae 618, 521-525.
Van Rensburg L, Peacock J, Krueger G. 1999. Boundary layer, stomatal geometry and-spacing in relation to drought tolerance in four Nicotiana tabacum L. cultivars. South African Journal of Plant and Soil 16(1), 44-49.
Wang X, Arora R, Horner HT, Krebs SL. 2008. Structural adaptations in overwintering leaves of thermonastic and nonthermonastic Rhododendron species. Journal of the American Society for Horticultural Science 133(6), 768-776.
Yemm EW, Willis AJ. 1954. The estimation of carbohydrates in plant extracts by anthrone. Biochemical journal 57(3), 508-514.