Influence of Plant Growth Regulators on Callus Induction, Silymarin Production and Antioxidant Activity in Silybum marianum L. Gaertn. by Tissue Culture

Ebrahimzadeh, Mohammad Hadi; Naseri, Mahboobeh; Younesikelaki, Fatemeh Soghra; Yusefi Javan, Iman; Swamy N, Rama

doi:10.22059/ijhst.2022.322832.465

Document Type : Research paper

Authors

¹ Department of Plant Production, Faculty of Agriculture, University of Torbat Heydarieh, Torbat Heydarieh, Iran

² Plant Production of University of Torbate Heydarieh, Iran

³ Department of Biology, Payame Noor University, Iran

⁴ University of Torbat heydarieh

⁵ DOS in Biotechnology, Kakatiya University, Telangana, India

https://doi.org/10.22059/ijhst.2022.322832.465

Abstract

Silybum marianum (L.) is a medicinally important herb known for producing the liver-protecting compound silymarin, an isomeric mixture of flavonolignans and taxifolin. In this study, a protocol (plant growth regulators in various concentrations and combinations of coconut water) for the initiation of callus and silymarin production using different types of explantes (root, hypocotyls, cotyledonary leaf) of S. marianum L. was established. The free radical scavenging activity of silymarin extracted from callus cultures of these explants was investigated. In comparison to other plant growth regulators and explants studied, root explants cultured on MS medium enriched with 5.0 M NAA + 2.5 M BAP + 10% coconut water produced the most friable callus and had the highest possible level of silymarin and free radical scavenging activity. NAA + BAP + coconut water was found to be the best hormonal combination for callus initiation and mass production. This research will aid in understanding the influence of plant growth regulators and different types of explantes on the production of valuable secondary metabolites in S. marianum L. in vitro cultures.

Keywords

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International Journal of Horticultural Science and Technology

Influence of Plant Growth Regulators on Callus Induction, Silymarin Production and Antioxidant Activity in Silybum marianum L. Gaertn. by Tissue Culture

References

References

Volume 10, Issue 2
April 2023
Pages 223-236

Influence of Plant Growth Regulators on Callus Induction, Silymarin Production and Antioxidant Activity in Silybum marianum L. Gaertn. by Tissue Culture

References

References

Volume 10, Issue 2April 2023Pages 223-236

Volume 10, Issue 2
April 2023
Pages 223-236