Document Type : Research paper


1 Department of Horticultural science and Agronomy, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Department of Horticultural Science, Agriculture Faculty, Shahid-Chamran University, Ahvaz, Iran

3 Department of Irrigation and Drainage, Water Science Engineering Faculty, Shahid-Chamran University, Ahvaz, Iran


Seed priming is one of the most important measures in propagation of plants by seed, which leads to increase of percentage and rate of germination. This study was conducted as a factorial arrangement in a completely randomised design on two farm cucumber cultivars (‘Impress’ and ‘Emperor’). Priming treatments were silicic acid (SA) and ascorbic acid (AsA) at three levels of 50, 100 and 150 mg L-1, pyridoxine at three levels of 0.02%, 0.04% and 0.06% and compound treatment (SA 75 mg L-1 + AsA 75 mg L-1 + pyridoxine 0.03%). There was a positive and significant correlation between the Catalase (CAT) enzyme activity in the radicle and plumule and Polyphenol oxidase (PPO) in the radicle and germination rate and percentage. In the ‘Impress’ cultivar, the highest CAT activity in the radical and plumule was observed after using of 150 mg L-1 AsA. The highest activity of Polyphenol oxidase (PPO) in the radical was detected after using of 0.04% pyridoxine. In the ‘Emperor’ cultivar, the highest CAT enzyme activity was detected in the radical and plumule, respectively by using of 75 mg L-1 AsA, 75 mg L-1 SA, and 0.03% pyridoxine. AsA (50 mg L-1) induced the highest activity of PPO enzyme in the radicle. Finally, to increase the antioxidant enzyme activity in cucumbers against unfavourable environmental conditions, our results confirmed the effectiveness of 150 mg L-1 AsA and 0.04% pyridoxine for the ‘Impress’ cultivar and the compound treatment and 50 mg L-1 AsA for the ‘Emparator’ cultivar.


Aebi H. 1983. Catalase, In: Bergmeyer H, ed., "Methods of Enzymatic Analysis 3. Verlag Chemie", Weinheim, Germany, 273-277.
Afzal I, Basra S, Farooq M, Nawaz A. 2009. Alleviation of salinity stress in spring wheat by hormonal priming whit ABC, salicylic acid and ascorbic acid. Agricultural and Biology 1, 23-28.
Agarwal S, Pandey V. 2004. Antioxidant enzyme responses to NaCl stress in CasSA angustifolia. Biologia Plantarum 48(4), 555-560.
Al-Hakimi AMA, Hamada AM. 2001. Counteraction of salinity stress on wheat plants by grain soaking in ascorbic acid, thiamin or sodium salicylate. Biologia Plantarum 44(2), 253-261. doi:10.1023/A:1010255526903.
Ashraf M, Foolad MR. 2005. Pre‐sowing seed treatment-A shotgun approach to improve germination, plant growth, and crop yield under saline and non‐saline conditions. Advances in Agronomy 88, 223-71.
Ashraf M, Rauf H. 2001. Inducing salt tolerance in maize (Zea mays L.) through seed priming with chloride salts: Growth and ion transport at early growth stages. Acta Physiologiae Plantarum 23(4), 407-414. doi:10.1007/s11738-001-0050-9.
Bankaji I, Ben Hammouda I, Sleimi N. 2017. Effect of priming on seed germination of Cucurbita Pepo under copper stress. American Journal of Life Science Researches 5(3), 118-123.
Basra SM, Farooq M, Rehman H, Saleem BA. 2007. Improving the germination and early seedling growth in melon (Cucumis melo L.) by pre-sowing salicylicate treatments. International Journal of Agriculture and Biology 10, 238-240.
Basra SMA, Ashraf M, Iqbal N, Khaliq A, Ahmad R. 2004. Physiological and biochemical aspect of pre-sowing heat stress on cotton seed. Seed Science and Technology 32(3), 756-774. doi:10.15258/sst.2004.32.3.12.
Bradford KJ. 2017. Water relations in seed germination. In: Seed development and germination (pp. 351-396). Routledge.
Burguieres E, McCu P, Kwon Y, Shetty K. 2007. Effect of vitamin C and folic acid on seed vigor response and phenolic-linked antioxidation activity. Bio-resource Technology 98(7), 1393-1404.
Daneshvar M. 2001. Growing vegetables, Shahid-Chamran University Press, 1st Ed (In PerSAn).
Farhoudi R, Saeedipour S, Mohammadreza D. 2011. The effect of NaCl seed priming on salt tolerance, antioxidant enzyme activity, and proline and carbohydrate accumulation of Muskmelon (Cucumis melo L.) under saline condition. African Journal of Agricultural Research 6(6), 1363-1370.
Giannopolitis C, Ries S. 1977. Superoxide dismutases I. Occurrence in higher plants. Plant Physiology 59, 309-314. doi:10.1104/pp.59.2.309.
Gul H, Ahmad R, Hamayun M. 2015. Impact of exogenously applied ascorbic acid on growth, some biochemical constituents and ionic composition of guar (Cymopsis tetragonoloba) subjected to salinity stress. Life Science 3(1), 22-40.
Hamama H, Murniati E. 2010. The effect of ascorbic acid treatment on viability and vigor maize (Zea mays L.) seedling under drought stress. Hayati Journal of Biosciences 17(3), 105-109.
Harris D, Pathan AK, Gothkar P, Joshi A, Chivasa W, Nyamudeza P. 2001. On-farm seed priming: using participatory methods to revive and refine a key technology. Agricultural Systems 69(1), 151-164. doi:10.1016/S0308-521X(01)00023-3.
Huang S, Li R, Zhang Z, Li L, Gu X, Fan W, Lucas WJ, Wang X, Xie B, Ni P, Ren Y. 2009. The genome of the cucumber, Cucumis sativus L. Nature Genetics 41(12), 1275-1281. doi:10.1038/ng.475.
Maguire JD. 1962. Speed of germination-Aaid in selection and evaluation for seedling emergence and vigor. Crop Science 2, 176-177.
McDonald MB. 1999. Seed deterioration: physiology, repair and assessment. Seed Science and Technology 27(1), 177-237.
Murungu FS, Nyamugafata P, Chiduza C, Clark LJ, Whalley WR. 2003. Effects of seed priming, aggregate size and soil matrix potential on emergence of cotton (Gossypium hirsutum L.) and maize (Zea mays L.). Soil and Tillage Research 74(2), 161-168. doi:10.1016/j.still.2003.06.003
Omidi H, Soroushzadeh A, Salehi A, Ghezeli FD. 2005. Rape seed germination as affected by osmo-priming pretreatment. Agricultural Sciences and Technology Journal 19(2), 125-136.
Pandey P, Bhanuprakash K, Umesh U. 2017. Effect of seed priming on seed germination and vigor in fresh and aged seeds of cucumber. International Journal of Environment, Agriculture and Biotechnology 2(4), 238900.
Rafique N, Raza SH, Qasim M, Iqbal NAEEM. 2011. Pre-sowing application of ascorbic acid and salicylic acid to seed of pumpkin and seedling response to salt. Pakistan Journal of Botany 43(6), 2677-2682.
Rehman H, Farooq M, Basra SMA, Afzal I. 2011. Hormonal priming with salicylic acid improves the emergence and early seedling growth in cucumber. Journal of Agriculture and Social Science 7, 109-113.
Rukui H, Sutevee S, Lop P, Sunanta J, Chaiwat C. 2006. Changes in antioxidant enzyme activity, lipid peroxidation and seedling growth of cucumber seed induced by hydro-priming and electric field treatments. Kasetsart Journal (Natural Science) 40, 825-834.
Shakirova FM, Sakhabutdinova AR, Bezrukova MV, Fatkhutdinova RA, Fatkhutdinova DR. 2003. Changes in the hormonal status of wheat seedlings induced by salicylic acid and salinity. Plant Science 164, 317-322. doi:10.1016/S0168-9452(02)00415-6.
Simova-Stoilova L, Demirevaska K, Petrova T, Tsenov N, Feller U. 2008. Antioxidative protection in wheat varieties under severe recoverable drought at seedling stage. Plant Soil and Environment 54, 529 -539.
Siosemardeh A, Sadeghi F, Kanooni H, BahramNezhad B, Gholami S. 2014. Effects of drought stress on physiologic features, seed yield and its components of cowpea genotypes (Cicer arietinum L.). Iranian Agronomy Science Journal 16(2). (In PerSAn)
Solvia RC, Elenz P, Sebastian M, Martin O. 2001. Evaluation of browning effect on avocado puree preserved by combined methods. Innovative Food Science and Emerging Technologies 1, 261-268.
Zanjan MG, Asli DE. 2012. A study of seed germination and early seeding growth of wheat genotypes affected by different seed pyridoxine-priming duration. Annals of Biological Research 3(12), 5687-5691.