Quantitative Assessment of Diosgenin from Different Ecotypes of Iranian Fenugreek (Trigonella foenum-graecum L.) by High-performance Liquid Chromatography

Document Type : Research paper

Authors

1 Department of Horticultural Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

2 Department of Horticultural Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.

3 Department of Plant Biotechnology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.

Abstract

In this study, a sensitive, accurate and reproducible high performance liquid chromatographic method was developed for determination of diosgenin from fenugreek (Trigonella foenum-graecum) leaves in 22 ecotypes of Iranian fenugreek. The obtained results showed that the highest concentration of diosgenin with 23.8 mg g-1dw was found in Boshruyeh ecotype. While, Mashhad ecotype with 3.51 mg g-1dw diosgenin contained the lowest concentration of diosgenin. The method employed in current study confirmed significant variations in the diosgenin concentration in Iranian fenugreek. Therefore, Iranian fenugreek can be considered as a new potential crop and source of diosgenin in the pharmaceutical industry.

Keywords

References

Ariburnu E, Uludag M.F, Yalcinkaya H, Yesilada E. 2012. Comparative determination of sibutramine as an adulterant in natural slimming products by HPLC and HPTLC densitometry. Journal of pharmaceutical and biomedical analysis 64, 77-81.
Dangi R, Misar A, Tamhankar S, Rao S. 2014. Diosgenin content in some Trigonella species. Indian Journal of Advances in plant Research 1, 47-51.
De Candolle A. 1886. Origin of cultivated plants (Hafner, New York, 1967). English translation of the second edition. 316-321.
Fazli F, Hardman R. 1968. The spice, fenugreek (Trigonella foenum-graecum L.): Its conmmercial varieties of seed as a source mehrafarin and authors of diosgenin. Tropical Science 10, 66-78. 
Fernandes P, Cruz A, Angelova B, Pinheiro H.M, Cabral J.M.S. 2003. Microbial conversion of steroid compounds: recent developments. Enzyme and Microbial Technology 32, 688-705.
Güçlü-Üstündağ Ö, Mazza G. 2007. Saponins: properties, applications and processing. Critical reviews in food science and nutrition 47, 231-258.
Huhman D.V, Sumner L.W. 2002. Metabolic profiling of saponins in Medicago sativa and Medicago truncatula using HPLC coupled to an electrospray ion-trap mass spectrometer. Phytochemistry 59, 347-360.
Hwang W.T, Kim Y, Hong C, Lee J, Park D. 2011. Simple stability-indicating validated HPLC method for diosgenin in cosmeceutical formulations with long term stability application for light and heat. International Journal of Pharmacy and Pharmaceutical Sciences3, 466-471.
Kalinowska M, Zimowski J, Pączkowski C, Wojciechowski Z.A. 2005. The formation of sugar chains in triterpenoid saponins and glycoalkaloids. Phytochemistry Reviews 4, 237-257.
Laila O, Murtaza I, Abdin M, Ahmad S, Ganai N.A, Jehangir M. 2014. Development and validation of HPTLC method for simultaneous estimation of diosgenin and quercetin in fenugreek seeds (Trigonella foenum-graceum). ISRN Chromatography.
Li P, Mao Z, Lou J, Li Y, Mou Y, Lu S, Peng Y, Zhou L. 2011. Enhancement of diosgenin production in Dioscorea zingiberensis cell cultures by oligosaccharides from its endophytic fungus Fusarium oxysporum Dzf17. Molecules 16, 10631-10644.
Li P, Mou Y, Lu S, Sun W, Lou J, Yin C, Zhou L. 2012. Quantitative determination of diosgenin in Dioscorea zingiberensis cell cultures by microplate-spectrophotometry and high-performance liquid chromatography. African journal of pharmacy and pharmacology 6, 1186-1193.
Martin E, Akan H, Ekici M, Aytac Z. 2011. New chromosome numbers in the genus Trigonella L.(Fabaceae) from Turkey. African journal of Biotechnology 10, 116.
Mathur V, Mathur N. 2005. Fenugreek and other lesser known legume galactomannan-polysaccharides: scope for developments. Journal of Scientific and Industrial research 64, 475.
Ressmann A.K, Strassl K, Gaertner P, Zhao B, Greiner L, Bica K. 2012. New aspects for biomass processing with ionic liquids: towards the isolation of pharmaceutically active betulin. Green Chemistry14, 940-944.
Rosser A. 1985. The day of the yam. Nursing times 81, 47.
Zhang C.L, Gao J.M, Zhu W. 2012. Steroidal saponins from the rhizomes and roots of Smilax scobinicaulis. Phytochemistry Letters 5, 49-52.
Zhu Y, Huang W, Ni J. 2010. A promising clean process for production of diosgenin from Dioscorea zingiberensis CH Wright. Journal of Cleaner Production18, 242-247.
International Journal of Horticultural Science and Technology
Volume 5, Issue 1
June 2018
Pages 103-109

Files

History

  • Receive Date: 24 February 2018
  • Revise Date: 14 April 2018
  • Accept Date: 15 April 2018

Share

How to cite

Statistics