Production of parental lines using temperature and radiation treatments in greenhouse cucumber (Cucumis sativus L.)

Articles in Press

Document Type : Research paper

Authors

1 PhD student, Department of Agricultural Engineering, Faculty of Agriculture and Natural Resources, University of Hormozgan, Hormozgan, Iran

2 Associate Professor, Department of Agricultural Engineering, Faculty of Agriculture and Natural Resources, Hormozgan University, Hormozgan, Iran

3 Professor of the Department of Culture, Tissue and Cells, Agricultural Biotechnology Research Institute, Agricultural Research, Education and Extension Organization, Iran

4 Researcher of Radiation Application Research Institute, Nuclear Science and Technology Research Institute, Atomic Energy Organization, Iran

10.22059/ijhst.2025.384440.947

Abstract

Haploid and double-haploid technology through gametophytic embryogenesis and the altered expression of genes involved in the emergence of important agricultural traits allows for utilizing the genetic potential of genotypes and provides a suitable strategy for hybrid seed production. To this aim, this research was conducted in two two-factor factorial experiments as a completely randomized design with three replications in the research greenhouses of the Southern Agricultural and Natural Resources Research Center of Kerman province in 2023. Five levels of genotypes were determined as the first factor, and pollen radiation at two doses (300 and 350 Gy) and two temperature treatments of 35 °C for 6 and 12 hours to sterilize greenhouse cucumber pollens were specified as the second factor. The results indicated that haploid seedlings were obtained through parthenogenesis induction in cucumber genotypes. One-third of the fruits after pollination with treated pollens contained at least one parthenogenetic embryo. Since an optimal radiation dose is a key influential factor on the efficiency of parthenogenetic embryo induction, the ploidy level in double-haploid seedlings treated with 300 Gy of gamma radiation (10.67) and 6-hour temperature treatment at 35 °C (8.68) led to the highest haploid induction. Half of the produced plants possessed the haploid chromosome number and a combination of normal chromosomes and mixoploid chromosomes. The chromosome number was doubled in haploid plants by the laboratory colchicine treatment at a concentration of 500 mg/l for 24 h. This method can be used to produce homozygous lines for use in cucumber breeding programs.

Keywords

International Journal of Horticultural Science and Technology

Articles in Press, Accepted Manuscript
Available Online from 01 May 2026

Files

History

  • Receive Date: 27 October 2024
  • Revise Date: 16 December 2024
  • Accept Date: 31 December 2024

Share

How to cite

Statistics