Seyed Abolfazl Hassani; Ali Salehi Sardoei; Hamideh Azad Ghouge Bigloo; Hadi Ghasemi; Amir Ghorbanzadeh
Abstract
Various markers can be used for accurate identifications of plant genotypes and cultivars. Since microsatellite markers of simple sequence repeats (SSRs) are abundant and reliably reproducible, 14 pairs were used for evaluating polymorphic levels among 33 apple genotypes. All 14 pairs of primers had ...
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Various markers can be used for accurate identifications of plant genotypes and cultivars. Since microsatellite markers of simple sequence repeats (SSRs) are abundant and reliably reproducible, 14 pairs were used for evaluating polymorphic levels among 33 apple genotypes. All 14 pairs of primers had high degrees of polymorphism, ranging from three alleles (in the case of primers CH01h01 and CH02d12) to 12 alleles in primer CH05d04. In total, 83 polymorphic alleles appeared in these 14 SSR loci (with an average of 5.92 alleles per gene locus), and the polymorphic information content averaged 0.71. Dendrograms for molecular data were drawn based on the UPGMA method, and genotypes were divided into six main groups. The genotypes of Shahrood 20 and Shahrood 21 (95%) had the highest similarity with each other, while Shahrood 3 and Palestinian Malayer (14%) had the lowest. Principal component analysis confirmed the results of cluster analysis to determine relationships between the genotypes.
Hamed Hassanzadeh Khankahdani; Abdoolnabi Bagheri
Abstract
Date palm (Phoenix dactylifera L.) is a monocot, dioecious and high longevity tropical plant greatly considered due to its high economic importance in Middle East. This investigation was carried out to detect genetic relations among 34 cultivars of Iranian date palm, both males and females, using ISSR ...
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Date palm (Phoenix dactylifera L.) is a monocot, dioecious and high longevity tropical plant greatly considered due to its high economic importance in Middle East. This investigation was carried out to detect genetic relations among 34 cultivars of Iranian date palm, both males and females, using ISSR markers. Accordingly, total DNA was extracted using CTAB method and was subjected to PCR amplification using 12 ISSR primers. Analysis of ISSR data indicated that PIC value varied from 0.3695 to 0.4998 with average of 0.4497. The highest PIC value belonged to (CT)10 G primer (0.4498). Totally, 112 alleles were recognized. According to morphological attributes and ISSR markers, the 34 studied date cultivars divided into eight main groups and different sub-groups, and no significant correlation between grouping made by morphological and molecular markers. Unlike ISSR markers that produced a clear-cut among male and female cultivars, no distinct border was seen among male and female cultivars using morphological attributes. Furthermore, PCA analysis confirmed the grouping made by ISSR markers. In conclusion, ISSR markers have been useful to separate date palm cultivars.
