Bayram Emin Develi; Natalia Miler
Abstract
In the present study, the influence of three-wide spectrum light-emitting diodes (LEDs), emitting warm light AP67 (moderate blue and red and low red:far-red), AP673L (high red and high red:far-red) and G2 (high red and low red:far-red) with different colours mixing blue, green, red and far-red was used ...
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In the present study, the influence of three-wide spectrum light-emitting diodes (LEDs), emitting warm light AP67 (moderate blue and red and low red:far-red), AP673L (high red and high red:far-red) and G2 (high red and low red:far-red) with different colours mixing blue, green, red and far-red was used for micropropagation of three Polish potato cultivars; ‘Aruba’, ‘Oberon’ and ‘Skawa’. The planlets were grown in a growth chamber with a 16-h photoperiod. The photosynthetic photon flux density was 30 μmol m-2 s-1. The tissue culture medium for shoot proliferation was a standard MS-based medium. Microtuberization was observed on MS medium supplemented with higher sugar content (40 m g/L-1). Light quality affected the number of microtubers (MTs) per plantlet, the volume (cm3) of MTs, the fresh weight (mg) of MTs, and the width (mm) of MTs. Different light qualities did not affect morphological parameters, including length (mm), perimeter (mm), and crosssection area (mm2) of microtubers, whereas the highest number of microtuber were detected under the effect of AP67 light quality. The morphological parameters mostly depended on cultivar. The lowest number of microtubers was observed in the G2 treatment. In conclusion, ‘Aruba’ and ‘Oberon’ with AP67 and AP673L, as well as LED lighting systems, were determined as most suitable in terms of the MT count per plantlet. Also, these variables were most suitable for MT volume (cm3), fresh weight (mg), and width (mm).
Amin Hassankhah; Kourosh Vahdati; Mahmoud Lotfi; Masoud Mirmasoumi; John Preece; Mohammad Hassan Assareh
Abstract
Plantlets grown in conventional tissue culture systems usually encounter physiological and anatomical abnormalities including inability to photosynthesize, low chlorophyll content, open stomata, lack of a cuticle layer in the leaf, abnormal xylem parenchyma etc. Photoautotrophic and photomixotrophic ...
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Plantlets grown in conventional tissue culture systems usually encounter physiological and anatomical abnormalities including inability to photosynthesize, low chlorophyll content, open stomata, lack of a cuticle layer in the leaf, abnormal xylem parenchyma etc. Photoautotrophic and photomixotrophic systems could diminish these problems. The purpose of this study was to increase the chlorophyll content and photosynthetic ability of shoots grown in vitro and to improve the adaptation of walnut plantlets. Walnut apical leaf buds were cultured in vessels containing DKW medium supplemented with 0, 15, 30 and 45 g L-1of sucrose. The vessels were closed with a clear polypropylene lid with two syringe filters on the lid (V1) or with a 50 mm microporous polypropylene membrane (V2). Natural ventilation had a significant effect on most of the growth indices. Furthermore, different levels of sucrose had significant effects on growth characteristics. Natural ventilation increased chlorophyll content significantly. By using ventilated vessels containing 15 g L-1 sucrose, the total chlorophyll was significantly increased. Stomata under non-ventilated conditions were spherical with wide openings, whereas those in ventilated vessels were elliptical with narrow openings. As a result, ventilated vessels with 15 g L-1of sucrose produced healthy plantlets.
Morteza Khosh-Khui
Abstract
Scented rose species are very important in the genus Rosa due to their use in highly-prized essential oil production and as garden roses. The fragrance of the rose flower (attar) captured by extraction is one of the most valuable flavor and fragrance products. A comprehensive study of the papers published ...
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Scented rose species are very important in the genus Rosa due to their use in highly-prized essential oil production and as garden roses. The fragrance of the rose flower (attar) captured by extraction is one of the most valuable flavor and fragrance products. A comprehensive study of the papers published in the field of in vitro culture and biotechnology of these species, particularly Damask rose, was performed. Then, in separate sections, the articles related to media preparation for callus culture, shoot regeneration and multiplication, rooting, and cell suspension culture were reviewed. In this paper, the stages of the in vitro culture of scented roses, including establishment, multiplication, in vitro rooting, acclimatization, and genetic variability are also considered. Subsequently, investigations on methods of in vitro culture of scented roses by callus, pollen, anther, cell suspension, and protoplast culture are described. Finally, technological improvements for micropropagation, biotechnology. pharmaceutical, and other properties of the Damask rose and other species of scented roses are discussed. Prospects of in vitro culture and biotechnological techniques of scented rose species are also discussed. Among scented roses, the focus of this paper is on four species, namely Rosa damascena Mill., R. gallica Linn., R. centifolia Linn., and R. moschata Herrm., which are highly prized for oil production.
