Seyed Mohammad Mahdi Mortazavian; Mahdieh Arshadi Bidgoli
Abstract
Cumin (Cuminum cyminum L.) is an annual, outcross plant with medicinal properties. It is adapted to arid and semi-arid regions of the world. This research aimed to evaluate genetic parameters such as GCA and genetic distance based on molecular markers and important traits for parent selection among diverse ...
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Cumin (Cuminum cyminum L.) is an annual, outcross plant with medicinal properties. It is adapted to arid and semi-arid regions of the world. This research aimed to evaluate genetic parameters such as GCA and genetic distance based on molecular markers and important traits for parent selection among diverse genotypes of cumin. For this purpose, ISSR markers and a polycross assay were conducted. Forty-nine half-sib families were obtained from the polycross test and were evaluated for two years under normal and drought stress conditions. The measurements were aimed at 1000-seed weight, mechanized harvest height, and seed yield. The statistical analysis guided a selection of elite parents due to the high genetic diversity among half-sib families. An estimation of narrow sense heritability and general combining ability of the studied traits revealed the contribution of both additive and non-additive effects in genetically controlling the evaluated traits. According to the general combining ability, drought tolerance indices and genetic distance of 49 genotypes, the top 10 candidates were selected for the production of drought-tolerant seeds. From a molecular perspective, the banding patterns obtained from ISSR markers showed significant genetic diversity among all genotypes and indicated a proper distance among ten selected parents. The findings of this experiment provided a starting point for cumin breeding. It is likely that a breeding program would proceed with random pollinations among the ten select genotypes under controlled conditions to produce high-yield drought-tolerant varieties.
Leila Baghazadeh Daryaii; Davood Samsampour; Abdolnabi Bagheri; Majid Askari Seyahooei; Mojdeh Raam
Abstract
Investigating genetic diversity in plant species provides a platform for further insight in plant breeding and conservation. Therefore, in the present study genetic diversity of 13 geographically isolated genotypes of Zhumeria majdea, as a seriously endangered medicine plant growing exclusively in Hormozgan ...
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Investigating genetic diversity in plant species provides a platform for further insight in plant breeding and conservation. Therefore, in the present study genetic diversity of 13 geographically isolated genotypes of Zhumeria majdea, as a seriously endangered medicine plant growing exclusively in Hormozgan province (South of Iran) was studied. To do so, the leaf samples of Z. majdea were collected from the main growing habitats of this spices including Haji Abad, Geno and Bastak regions. The collected leaf samples were subjected to DNA extraction followed by PCR assay, using Inter-Simple Sequence Repeats (ISSR) markers. Twelve markers produced totally 121 polymorphic bands and revealed a clear-cut among and within Z. majdea genotypes. The analysis of molecular variance (AMOVA) showed 86% and 14% variations within and among populations, respectively. Cluster analysis divided genotypes into four main groups. The first and second principle coordinates allocated 28.81% and 15.71% of the variations, respectively. In addition to the innate differences of the individuals, the high intra population variation of Z. majdea, can also be explained by differences in the presence or absence of endophytes and differences in the type and genetic pattern of their endophytes.
