Mehdi Garazhian; Ali Gharaghani; Saeid Eshghi; Ahmad Tahmasebi
Abstract
This study was carried out to estimate the genetic diversity and relationships of 74 Iranian blackberry accessions assigned to 5 different species using inter-simple sequence repeats (ISSR) marker analysis and morphological trait characterization. Sixteen traits were analyzed, including phenological, ...
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This study was carried out to estimate the genetic diversity and relationships of 74 Iranian blackberry accessions assigned to 5 different species using inter-simple sequence repeats (ISSR) marker analysis and morphological trait characterization. Sixteen traits were analyzed, including phenological, vegetative and reproductive attributes (in 57 out of 74 accessions), and 10 ISSR primers were screened (in 74 accessions). The yield and leaf width had the highest and lowest genetic diversity, (diversity index = 62.57 and 13.74), respectively. Flowering and ripening date were recorded as traits with the strongest correlations (r = 0.98). Ten ISSR primers were selected and produced a total of 161 amplified fragments (200 to 3500 bp), of which 113 were polymorphic. The highest, lowest and average PIC values were 0.53, 0.38 and 0.44, respectively. Principle component analysis (PCA) was based on morphological traits and showed that the first six components explained 84.9% of the variations among the traits. Meanwhile, the principal coordinate analysis (PCoA) was based on ISSR data and implied the first eight principal coordinates which explained 67.06% of the total variation. Cluster analysis was based on morphological traits and ISSR data ultimately classified all accessions into two and three major groups, respectively, so that the distribution pattern of genotypes was mainly based on species and the geographic origins.
Hesamaddin Afshari-Jafarbigloo; Saeid Eshghi; Ali Gharaghani
Abstract
Some cultivars of grapevine have very compact cluster which adversely affect its quality and marketability. ‘Yaghouti’ is a commercial early table grape cultivar that has very compact clusters. The present study was conducted to assess the effects of mechanical and chemical thinning as well ...
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Some cultivars of grapevine have very compact cluster which adversely affect its quality and marketability. ‘Yaghouti’ is a commercial early table grape cultivar that has very compact clusters. The present study was conducted to assess the effects of mechanical and chemical thinning as well as GA3 application on cluster morphology and berry size of grapevine cv. ‘Yaghouti’. Treatments were chemical thinning agents including: NAA (7.5 and 15 mg/L), Ethephon (75 and 150 mg/L), Sevin (750 and 1500 mg/L) and mechanical thinning by a comb (comb thinning), applied a week after fruit set and unthinned clusters as control with 3 replications, 10 days after thinning, clusters were immersed in GA3 solution at 60 mg/L. Results indicated that berry numbers were reduced in all thinning treatments. Length, width and weight of berries increased significantly when treated with hand or chemical thinning. GA3 alone and Sevin at 750 mg/L in combination with GA3 treatment improved yield and the quality of berries. Total phenolics compounds, TSS and berries colour were improved in all thinning treatments. In general, application of Sevin at 750 mg/L combined with GA3 and hand thinning along with GA3 are proposed to improve quality of grapevine berries.
Mehdi Abdolmaleki; Morteza Khosh-Khui; Saeid Eshghi; Asghar Ramezanian
Abstract
Rosa hybrida L. is an important commercial cut flower. Salicylic acid (SA) and calcium chloride (CaCl2) act as endogenous signal molecules responsible for growth parameters in plants. The aim of this study was to evaluate the effects of preharvest SA and CaCl2 treatments in extending the vase life of ...
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Rosa hybrida L. is an important commercial cut flower. Salicylic acid (SA) and calcium chloride (CaCl2) act as endogenous signal molecules responsible for growth parameters in plants. The aim of this study was to evaluate the effects of preharvest SA and CaCl2 treatments in extending the vase life of cut rose flowers. Therefore, a factorial experiment based on completely randomized design with SA (0, 150, 300, and 450 mg L-1) and CaCl2 (0, 0.75, 1.5, and 2.25%) with 4 replicates and 2 samples (individual flowers) in each replicate, was conducted. Changes in growth, macronutrient concentration, chlorophyll content, leaf relative water content (LRWC), flower quality, vase life, and membrane stability index were investigated in R. hybrida cv. “Dolce Vita.” Exogenously applied SA and CaCl2 increased plant growth (such as shoots and flower buds). Foliar application of SA and CaCl2 also increased macronutrient concentration (N, K, Ca, and Mg), chlorophyll content, LRWC, flower quality, and vase life; however membrane stability index was decreased with increasing levels of SA and CaCl2. These results suggest that SA and CaCl2 could be used as potential growth promoters to improve postharvest life of roses. According to the results of this experiment, SA and CaCl2 as natural, cheap, safe, and biodegradable compounds are suitable alternatives for conventional chemical treatments in order to prolong vase life of cut rose flowers. Commercialization of these compounds for optimum formulations needs further experiments.
Saeid Eshghi; Loghman Salehi; Mohamad Javad Karami
Abstract
Grapes are significant sources of nutritional antioxidants as well as biologically active dietary components. This study was carried out to determine the amount of total phenols, anthocyanins and antioxidant activity of 35 grapevine (Vitis vinifera L.) cultivars grown in Fars province (Iran). Ripened ...
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Grapes are significant sources of nutritional antioxidants as well as biologically active dietary components. This study was carried out to determine the amount of total phenols, anthocyanins and antioxidant activity of 35 grapevine (Vitis vinifera L.) cultivars grown in Fars province (Iran). Ripened bunches were randomly harvested from grapevine collection in Zarghan (Fars province, Iran) Agricultural Research Center, and then were transferred to the laboratory. Berry size (length, diameter, and weight), skin weight, acidity, vitamin C, total soluble solids (TSS), pH, phenols, anthocyanins and antioxidant activity were evaluated. The results showed that total phenols, anthocyanin and antioxidant activity in the berries varied among the investigated cultivars. ‘Gandome Uromia’ (Red, 64) and ‘Rishbaba Uromia’ (Red, 75) cultivars had the highest values of antioxidant activity and total phenols. The lowest amount of phenol was obtained from ‘Divaneh Kashmar’ (White, 135) cultivar. Anthocyanin and antioxidant activity were the lowest in ‘Jeshnion Bavanat’ (White, 105) cultivar. Antioxidant activity had a positive significant correlation with amount of phenols and anthocyanin. In general, it was found that different cultivars in this study had a vast range of antioxidant activity from 14.55 to 66.47%.
