Genetic Linkage Map of Oriental × Oriental Lily Population via AFLP and SSR Markers

Document Type : Research paper

Authors

1 Department of Horticulture and Landscape Engineering, Sayyed Jamaleddin Asadabadi University, Asadabad, Iran

2 Department of Horticulture, Faculty of Agriculture and Natural Resources, Mohaghegh Ardabili University, Ardabil, Iran

3 Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China

10.22059/ijhst.2024.371709.764

Abstract

Linkage maps are the framework for using genetic markers in biotechnological programs. In this experiment, two widely used markers were applied to find the loci of some momentous ornamental traits in lily flowers. A mapping population of 100 F1 progeny was used to construct the genetic linkage maps using the CP (out breeder full-sib family) model of JoinMap4 software. A total of 940 primers were tested, and the best ones were 172 primer pairs, including 96 AFLP Marker and 76 SSR markers used for map construction. A total of 616 loci with 465 loci for the AFLP marker and 151 the loci for SSR marker were scored. The whole mapped length was 2144.2 cM. The overall number of mapped loci was 189 loci of which 152 loci were assessed by AFLP marker and 47 loci by SSR marker. The full length of linkage groups for the maternal map was 861.6 cM with 12 linkage groups. The shortest and longest linkage groups gap in the maternal maps also was 2.2 cM and 55.7 cm, respectively. The numbers of mapped loci in maternal genetic linkage maps were 52 loci with 42 loci for the AFLP marker and 10 loci for the SSR marker. In integrated plants, 6 linkage groups were found with 86 completely assessed loci. The total length of the linkage group for integrated maps was 606.6 cM. The shortest and longest gap of LGs was also 0.2 cM and 26.8 cm, respectively.

Keywords

International Journal of Horticultural Science and Technology
Volume 12, Issue 4
September 2025
Pages 477-492

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History

  • Receive Date: 28 January 2024
  • Revise Date: 27 April 2024
  • Accept Date: 01 June 2024

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