Seed Priming Mellissa officinalis L. with Potassium Nitrate and Inoculation with Plant Growth Promoting Rhizobacteria at Different Times

Document Type : Research paper

Authors

1 Ph.D student, Department of Horticulture Sciences, Faculty of Agriculture, Lorestan University, Khorram abad, Iran.

2 Assistant Professor in Botany Departmentof Botany, University of Chakwal, Chakwal, Pakistan

10.22059/ijhst.2024.366314.706

Abstract

Priming seed priming and seedling inoculation with bio elicitor improve growth and phytochemical constituents of medicinal plants. This study was conducted with the aim of investigate the role of seed priming with potassium nitrate (0, 0.25, 0.5, 0.75 and 1%) at different times and inoculation of seedling originated from primed seeds with rhizobacteria strains (Pseudomonas fluorescens and P. putida) on physiological and metabolic attributes of Melissa officinalis L. The results showed that the interaction of treatments on morphological, physiological, photosynthetic pigments, metabolic traits contain percentage and essential oil composition were significant at 0.25% concentration of potassium nitrate with 72 hours priming of P. putida bacteria inoculation. The phenolic contents increased (56.30 mg. g-1 of gallic acid per ml of extract) in inoculation of P. putida and P. fluorescens bacteria while the highest antioxidant activity (50.05 mg. ml-1) was noticed in inoculum of P. fluorescens bacteria with 12 hours of priming. In addition, remarkable increase of 20% was observed in essential oils and related compounds such as carvacrol, thymol and isomenthone. Overall, seed priming along with seedling inoculation with pseudomonas strains play vital role in the increase in both primary and secondary metabolites of lemon balm plants.

Keywords



Articles in Press, Accepted Manuscript
Available Online from 01 January 2025
  • Receive Date: 07 October 2023
  • Revise Date: 31 May 2024
  • Accept Date: 07 June 2024