Effects of Selected Plant Growth Stimulators on Enhancing Germinability and Germination Parameters of Zea mays L. under Microgravity Conditions Simulated by a Two-Dimensional Clinostat

Saheed, Musa Ibrahim; Beckley, Ikhajiagbe; Anoliefo, Geoffrey O.; Orukpe, Alexander O.

doi:10.22059/ijhst.2022.334591.522

Document Type : Research paper

Authors

¹ Department of Biology and Forensic Science, Admiralty University of Nigeria, Delta State Nigeria

² Department of Plant Biology and Biotechnology, University of Benin, Benin City Nigeria

³ Department of Plant Biology and Biotechnology, University of Benin, Benin City Nigeria.

⁴ Department of Plant Biology and Biotechnology, University of Benin. Benin City.

https://doi.org/10.22059/ijhst.2022.334591.522

Abstract

The Earth has become increasingly overcrowded as a result of rapid urbanization and population growth, with strong predictions that its carrying capacity could be overstretched soon. As a result, it is important to test the possibilities of growing plants under space exploration conditions, especially gravitational balance. Since microgravity impedes plant development, it is important to evaluate the extents by which plant growth stimulators can reverse or enhance this trend. A total of 12 maize seeds were weighed and placed sideways in petri dishes and inoculated with plant growth stimulators, indole acetic acid (IAA), gibberellic acid (GA), and ascorbate (AA). They were clinorotated at different rates (0.5, 1.0, and 2.0 rpm), while the control seeds were just placed on a balanced table. Results of this research showed that under microgravity, the maize seeds had a decreased level of germination percentage with increasing clinorotation rates at 72 hrs, compared to the control group. But when stimulated with IAA, GA and AA, they improved in germination percentage, compared to the control, even under microgravity conditions. The seedling dry weight, germination time and other germination parameters also showed similar improvements. Comparatively, the three growth stimulators showed no major variations in their ability to improve germination percentage under micro-gravitational impact. However, IAA caused more improvements in seedling vigor, compared to the other growth regulators, while GA had more effects on the rate of germination. This research confirmed the possibilities of improving germinability in maize seeds under space exploration conditions.

Keywords

References

Al-Mudaris M. 1998. Notes on various parameters recording the speed of seed germination. Der Tropenlandwirt-Journal of Agriculture in the Tropics and Subtropics 99, 147–154.

2. AOSA. 1983. Seed Vigor Testing Handbook. Ithaca, NY, USA: Association of Official Seed Analysts.

3. Aravind J, Vimala Devi S, Radhamani J, Jacob S, Kalyani S. 2020. The germination metrics Package: A Brief Introduction. ICAR-National Bureau of Plant Genetic Resources, New Delhi. 46p.

4. Aronne G, De Micco V, Ariaudo P, De Pascale S. 2003. The effect of uni-axial clinostatrotation on germination and root anatomy of Phaseolus vulgaris L., Plant Biosystems 137 (2), 55–162.

5. Barth C, Mario DT. 2006. The Role of Ascorbic Acid in the Control of Flowering Tim and the Onset of senescence. Journal of Experimental Botany 57, 1657- 1665.

6. Basra A, Farooq I, Afzal I, Hussain M. 2006. Influence of osmo priming on the germination and early seedling growth of coarse and fine rice. International Journal of Agricultural Biology 8, 19–21.

7. Becerra-Vázquez Á, Coates R, Sánchez-Nieto S. 2020. Effects of seed priming on germination and seedling growth of desiccation-sensitive seeds from Mexican tropical rainforest. Journal of Plant Resources 133, 855–872. https://doi.org/10.1007/s10265-020- 01220-0

8. Bradford J. 1995. Relations in seed germination in Seed Development and Germination, J. Kigel and G. Galili, Eds., pp.351–396, Marcel Dekker, New York, NY, USA.

9. Bradford J. 2006. Manipulation of seed water relations via osmotic priming to improve germination under stress conditions. Horticultural Science 21, 1105–1112.

10. Braun M, Böhmer M, Häder D, Hemmersbach R, Palme K. 2018. Gravitational Biology I: Gravity Sensing and Graviorientation in Microorganisms and Plants (Cham, Switzerland: Springer International Publishing). https://www.springer.com/la/book/978 3319938936.

11. Cowles J, Lemay R, Jahns G. 2008. Microgravity effects on plant growth and lignification. Astrophys Lett Commun 27, 223-8. PMID: 11539286.

12. De Micco V, De Pascale S, Paradiso R, Aronne G. 2013. Microgravity effects on different stages of higher plant life cycle and completion of the seed-to-seed cycle. Plant Biology 1, 31-8. doi: 10.1111/plb.12098.

13. Edwards T. 1932. Temperature relations of seed germination. The Quarterly Review of Biology 7, 428– 443.

14. Farooq M, Ali AB, Sardar AC. 2013. Application of Allelopathy in crop production. International Journal of Agricultural and Biology 15, 1367-1378.

15. Farooq M, Basra S, Ahmad N, Hafeez K. 2005. Thermal hardening: A new seed vigor enhancement tool in rice. Journal of Integrative Plant Biology 47, 187–193. doi:10.1111/J.1744- 7909.2005.00031.x.

16. Gupta P, Mukherjee D. 1982. Influence of GA3 presoaking of seeds on biochemical changes in seedling parts of Pennisetum typhoides Rich. Proceedings of Indian National Science Academy 48 (5), 642–648.

17. Hedden P, Phillips A. 2000. Gibberellin metabolism: new insights revealed by the genes. Trends in Plant Science 5(12), 523–530.

18. Hoson T, Kamisaka S, Masuda Y. 1992. Changes in plant growth processes under microgravity conditions simulated by a three-dimensional clinostat. Botany Management Tokyo 105, 53–70. https://doi.org/10.1007/BF02489403.

19. Ikhajiagbe B, Mgbeze G, Folu M, Dania O. 2007. Responses of Sphenostylis stenocarpa (Hochst Ex A. Rich) Harms (African yam bean) to salinity stress I: Germination and vegetative growth. Nigerian Journal of Botany 20(1), 69-82.

20. Ikhajiagbe B, Musa S. 2020. Application of biosynthesized nanoparticles in the enhancement of growth and yield performances of Rice (Oryza sativa var. Nerica) under salinity conditions in a ferruginous ultisol. FUDMA Journal of Sciences (FJS) 4(1), 120-132.

21. ISTA. 2015. The germination test. International Rules for Seed Testing. International Seed Testing Association, Zurich, Switzerland. 1, 5–56.

22. Kapgate H, Potkile N, Zode N, Dhopte A. 1989. Persistence of Physiological Responses of upland Cotton to growth regulators. Annual Plant Physiology 3, 188-195.

23. Kering M, Zhang B. 2015. Effect of priming and seed size on germination and Emergence of Six Food-Type Soybean Varieties. International Journal of Agronomy 12, 6-16. https://doi.org/10.1155/2015/859212

24. Kiss J, Wolverton C, Wyatt S, Hasenstein KH, Van Loon J. 2019. Comparison of microgravity analogs to spaceflight in studies of plant growth and development. Frontiers of Plant Science 3, 12- 22. https://doi.org/10.3389/fpls.2019.01577

25. Koryum E, Chapman D. 2017. Plants and microgravity: Patterns of microgravity effects at the cellular and molecular levels. Cytology and Genetics. 51, 108–116. https://doi.org/10.3103/S0095452717020049

26. Lee H. 1990. Effect of Pre sewing seed with GA3 and IAA on flowering and yield component in Groundnut. Korean Journal of Crop Science. 35, 1-9.

27. Levine HG. 2010. The Influence of microgravity on plants. Assessed on 19th April 2021, at: https://www.nasa.gov/pdf/478076main_Day1_P03c_ Levine_Plants.pdf

28. Monje, O, Porterfield D, Stutte G. 2000. Boundary Layers around plant leaf and roots tissues depend on gravity. Gravity Space Biology Bulleting. 3, 14-25.

29. Morrow R, Remiker M, Tuominem L, Lee M, Crabb T. 2005. A low equivalent system mass plant growth Unit for Space exploration. 35th International Conference on Environmental Systems. Rome, Italy. PP. 56-64.

30. Mshelbula B, Okooboh G, Mensah J, Ikhajiagbe B, Zakariya R. 2015. The effects of indole-3-acetic acid (IAA) on the growth and yield of sesame (Sesamum indicum L.) under drought conditions. International Journal of Science and Knowledge 4(1), 60 -65.

31. Murungu F, Chiduza C, Nyamugafata, P, Clark, L, Whalley W, Finch-Savage, W. 2004. Effects of on-farm seed priming on consecutive daily sowing occasions on the emergence and growth of maize in semi-arid Zimbabwe. Field Crops Research 89(1), 49–57, 2004.

32. Musa S, Ikhajiagbe B. 2021. The growth response of rice (Oryza sativa L. var. FARO 44) in vitro after inoculation with bacterial isolates from a typical ferruginous ultisol. Bulletin of the National Research Centre 45(20), 1 – 20. https://doi.org/10.1186/s42269-021-00528-8

33. Orukpe AO, Anoliefo GO, Ikhajiagbe B. 2021. Effects of Clinorotation on the Enzyme Activities and Morphology of Zea mays Seedlings. American Journal of Life Sciences 9 (1), 11-18. DOI: 10.11648/j.ajls.20210901.13

34. Papaseit C, Pochon N, Tabony J. 2000. Microtubule selforganization is gravity-dependent. Proceedings of National Academy of Science, USA 97, 8364^8368.

35. Ranal M, Santana D. 2006. How and why to measure the germination process? Brazilian Journal of Botany 29, 1– 11. doi:10.1590/s0100-84042006000100002.

36. Raymond M. 2017. The Role of Plants in Space Exploration. National Aeronautic and Space Administration Exploration Research and Technology, Kennedy Space Centre, Florida, USA. p24.

37. Sevik H, Guney K. 2013. Effects of IAA, IBA, NAA, and GA3 on rooting and morphological features of Melissa officinalis L. stem cuttings. The Scientific World Journal 2, 55-59.

38. Shah S. 2004. Morpho-physiological Responses of Black Cumin Nigella Sativa L. Nitrogen, Gibberellic Acid and Kinetin Application. PhD Thesis. Aligar Muslim University, Aligar, India.

39. Smirnorf N, Wheeler GL. 2000. Ascorbic acid in plant: Biosynthesis and function. Crit. Rev. Biochemistry and Molecular Biology 35, 291-314.

40. Soga L. 2002. Growth stimulation of plants under microgravity conditions in space. Biological Sciences Space. 16(3), 117-8.

41. Soga K, Wakabayashi K, Kamisaka S. 2002. Stimulation of elongation growth and xyloglucan breakdown in Arabidopsis hypocotyls under microgravity conditions in space. Planta 215, 1040–1046. https://doi.org/10.1007/s00425-002-0838-x

42. Stutte G, Monje O, Goins, G, Ruffe G. 2000. Evapotranspiration and photosynthesis characteristics of two wheat cultivars grown the biomass production system. SAE Technical Paper 2001-0202180.

43. Vandenbrink J, Kiss J, Herranz R, Medina F. 2014. Light and gravity signals synergize in modulating plant development. Frontiers of Plant Science 5, 563. doi: 10.3389/fpls.2014.00563

44. Zabel P, Bamsey M, Schubert D, Tajmar M. 2016. Review and analysis of over 40 years of space plant growth systems. Life Sciences in Space Research 10:1-16.

45. Zhang Y, Ni Z, Yao X, Nie Y, Sun Q. 2007. Gibberellins and heterosis of plant height in wheat (Triticum aestivum L.),” BMC Genetics 8(40), 112-121.

International Journal of Horticultural Science and Technology

Effects of Selected Plant Growth Stimulators on Enhancing Germinability and Germination Parameters of Zea mays L. under Microgravity Conditions Simulated by a Two-Dimensional Clinostat

References

References

Volume 10, Issue 1
January 2023
Pages 1-10

Effects of Selected Plant Growth Stimulators on Enhancing Germinability and Germination Parameters of Zea mays L. under Microgravity Conditions Simulated by a Two-Dimensional Clinostat

References

References

Volume 10, Issue 1January 2023Pages 1-10

Volume 10, Issue 1
January 2023
Pages 1-10