Document Type : Research paper


Department of Horticultural Sciences, College of Agriculture, Vali-e-Asr University of Rafsanjan, Kerman, Iran



The efficiency of sulfur in controlling pistachio pests, especially psylla, has been documented in the available literature, but it is essential for pesticides not to affect the quality of the product. In this study, movento (0.5 L/1000 L), confidor (0.4 L/1000 L) and refinery micronized (R) and mineral (M) sulfur (30 and 60 kg/1000 L in water) were sprayed twice on pistachio trees of the cultivar ‘Ahmadaghaei’ (in mid-May and 50 days thereafter). Pistachios were harvested in late September and were stored at 4 °C for 25 and 50 days to evaluate the effects of pesticides on the quality of hulls and kernels. The taste, aroma, appearance, respiration rate, firmness of hull and kernel, a* (red-green), b* (blue-yellow) and chroma of hull and kernel declined over time, while L* (lightness) and the hue angle of the hull and kernel increased. Ethylene production remained stable over time. Chemical pesticides stimulated hull firmness. Refined micronized sulfur at both concentrations caused higher L* and hue angle, but lower a*, b* and chroma in the hull and kernel (i.e. lighter and yellower in color). All sulfur treatments reduced the firmness of the hull and kernel, while also increasing the respiration rate. R30 preserved the taste and aroma after 50 days. R30 and M30 showed higher ethylene production. The R30 treatment had some positive effects on preserving the taste and aroma of pistachios overtime. Thus, it may be recommended as a reliable treatment, although the ability of sulfur in pest control could be offset by the side effects of sulfur on product quality.
R30: Refined micronized sulfur (30 kg/1000 L); R60: Refinery micronized sulfur (60 kg/1000 L); M30: Mineral sulfur (30 kg/1000 L); M60: Mineral sulfur (60 kg/1000 L)


Afrousheh M, Hasheminasab H. 2018. Sulfur application as pesticide in pistachio orchard: health and safety. Pistachio and Health Journal 1(3), 52-63.
Agrawal M, Deepak SS. 2003. Physiological and biochemical responses of two cultivars of wheat to elevated levels of CO2 and SO2, singly and in combination. Environmental Pollution 121, 189–197.
Binam. 2017. Use of sulfur against psylla. Iran Pistachio Association Monthly, No. 16.
Cantin CM, Minas IS, Goulas V, Jimenez M, Manganaris GA, Michailides TJ, Crisosto CH. 2012. Sulfur dioxide fumigation alone or in combination with CO2-enriched atmosphere extends the market life of highbush blueberry fruit. Postharvest Biology and Technology 67, 84-91.
Culbreath AK, Brenneman TB, Kemerait RC, Stevenson KL, Anco DJ. 2019. Combinations of elemental sulfur with demethylation inhibitor fungicides for management of late leaf spot (Nothopassalora personata) of peanut. Crop Protection 125, 1-7.
Gheysarbigi S, Mirdehghan SH, Ghasemnezhad M, Nazoori F. 2020. The inhibitory effect of nitric oxide on enzymatic browning reactions of inpackage fresh pistachios (Pistacia vera L.). Postharvest Biology and Technology 159, 1-7.
Gong Young BAE, Noriaki K, Nobuyoshi N, Kozo I. 1995. Ethylene production in tomato plants by SO2 in relation to leaf injury. Journal of Japan Society for Atmospheric Environment / Taiki Kankyo Gakkaishi 30(6), 367-373.
Holb IJ, Kunz S. 2016. Integrated control of apple scab and powdery mildew in an organic apple orchard by combining potassium carbonates with wettable sulfur, pruning, and cultivar susceptibility. Phytofrontiers 100(9), 1894-1905.
Holb IJ, Schnabel G. 2008. A detached fruit study on the post-inoculation activity of lime sulfur against brown rot of peach (Monilinia fructicola). Australasian Plant Pathology 37, 454–459.
Hosseininia A, Edrisi B, Etaati M, KazemiSiahouei GHR. 2008. Simultaneous control of rose powdery mildew and rose spider mites by neem (Azadirachta indica Adr. Juss) seed oil, sulfur and dinocap. Pajouhesh Va Sazandegi 21(1), 34-40.
Kwasniewski MT, Sacks GL, Wilcox WF. 2014. Persistence of elemental sulfur spray residue on grapes during ripening and vinification. The American Journal of Enology and Viticulture 65(4), 453-462.
Li L, Yi H. 2020. Photosynthetic responses of Arabidopsis to SO2 were related to photosynthetic pigments, photosynthesis gene expression and redox regulation. Ecotoxicology and Environmental Safety. 203(15), 111019.
Mehrnejad MR. 2002. Bionomics of the common pistachio psylla, Agonoscena pistaciae, in Iran. Acta Horticulture 591, 535-539.
Moradinezhad F, Khayyat M, Ranjbari F, Maraki Z. 2019. Vacuum packaging optimises quality and reduces postharvest losses of pomegranate fruits. Journal of Horticulture and Postharvest Research 2 (Special Issue - Postharvest Losses), 15–26.
Pakrah S, Rahemi M, Nabipour A, Zahedzadeh F, Kakavand F, Vahdati K. 2021. Sensory and nutritional attributes of Persian walnut kernel influenced by maturity stage, drying method, and cultivar. Journal of Food Processing and Preservation 45(6), e15513.
Paniagua AC, East AR, Hindmarsh JP, Heyes JA. 2013. Moisture loss is the major cause of firmness change during postharvest storage of blueberry. Postharvest Biology and Technology 79, 13-19.
Peiser GD, Yang SF. 1979. Ethylene and ethane production from sulfur dioxide-injured plants. Plant Physiology 63, 142-145.
Recalde-Manrique L, Diaz-Miguel M . 1981 . Evolution of
Nazoori, ZamaniBahramabadi and Mirdehghan Int. J. Hort. Sci. Technol. 2022 9(4): 453-462
ethylene by sulphur dust addition. Physiologia Plantarum 53, 462-467.
Rivera SA, Zoffoli JP, Latorre BA. 2013. Determination of optimal sulfur dioxide time and concentration product for postharvest control of gray mold of blueberry fruit. Postharvest Biology and Technology 83, 40-46.
Romero SM, Patriarca A, Fernandez Pinto V, Vaamonde G. 2007. Effect of water activity and temperature on growth of ochratoxigenic strains of Aspergillus carbonarius isolated from Argentinean dried vine fruits. International journal of food microbiology 115, 140-143.
Rossello C, Mulet A, Simal S, Torres A, Caiiellas J. 1994. Quality of dried apricots: effect of storage temperature, light and SO2 content. Journal of the Science of Food and Agriculture 65, 121-124.
Sheikhi A, Mirdehghan SH, Arab MM, Eftekhari M, Ahmadi H, Jamshidi S, Gheysarbigi S. 2020. Novelorganic -based postharvest sanitizerfor mulationusing Box Behnken design and mathematical modeling approach: A case study of fresh pistachio storage under modified atmosphere packaging. Postharvest Biology and Technology 160, 111047.
Sheikhi A, Mirdehghan SH, Ferguson L. 2019. Extending storage potential of de-hulled fresh pistachios in passive-modified atmosphere. Journal of the Science of Food and Agriculture 99(7), 3426-3433.
Slade L, Levine H, Reid DS. 1991. Beyond water activity: Recent advances based on an alternative approach to the assessment of food quality and safety. Critical Reviews in Food Science and Nutrition 30, 115-360.
Wyk SV, Farid MM, Silva FVM. 2018. SO2, high pressure processing and pulsed electric field treatments of red wine: Effect on sensory, Brettanomyces inactivation and other quality parameters during one-year storage. Innovative Food Science & Emerging Technologies 48, 204-211.