Document Type : Research paper

Authors

1 Department of Horticulture, Faculty of Agricultural Sciences and Natural Resources, University of Mohaghegh Ardabili, Iran

2 Department of Horticulture, Faculty of Agricultural Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Iran

3 Ph.D Student of Medicinal Plants, Faculty of Plant Production, Gorgan Agricultural Sciences and Natural Resources University

10.22059/ijhst.2021.313970.416

Abstract

Bellis perennis is an ornamental, medicinal plant with a beautiful appearance. The current study aimed to assess the potential of daisy flowers cv. ‘Habanera’ to synthesize secondary metabolites after being affected by nano-phosphorus. The experiment was performed using a completely randomized design with three replications in greenhouse conditions. To achieve the research objectives, a range of parameters were observed, including total phenol content, total flavonoid content, and free radical scavenging activity in different organs (i.e. flower, leaf, and root), as well as photosynthetic pigments, anthocyanin, and quercetin contents. The results indicated that nano-phosphorus had significant effects on the said parameters (p< 0.05 and 0.01). Chlorophyll A, total chlorophyll, and carotenoids reached maximum content in response to the 1.5 g L-1 treatment. Nonetheless, the total phenol content in petals, free radical scavenging capacity, anthocyanin content and quercetin content were adversely affected by the nano-phosphorus treatment and, in fact, the said parameters showed higher contents in the control treatment. The application of nano-phosphorus fertilizer (3 g L-1) caused the highest flavonoid content in the leaves. At a concentration of 0.5 g L-1, it increased total flavonoid content and total phenols significantly in the roots. Phosphorus is necessary for producing primary and secondary metabolites, phospholipids, and nucleic acids in plants. Thus, its functions can vary greatly in plant organs.

Keywords

Astley S B. 2003. Dietary Antioxidants—Past, Present, and Future?" Trends in Food Science and Technology 14, 93–98.
Auffan M, Bottero J.Y, Chaneac C, Rose J. 2010. Inorganic Manufactured Nanoparticles: How Their Physicochemical Properties Influence Their Biological Effects in Aqueous Environments. Nanomedicine 5, 999–1007.
Baba S.A, Malik A. 2015. Determination of Total Phenolic and Flavonoid Content, Antimicrobial and Antioxidant Activity of a Root Extract of Arisaema Jacquemontii Blume. Journal of Taibah University for Science 9 (4), 449–454.
Barker A.V, Pilbeam D.J. 2015. Handbook of Plant Nutrition. CRC press. Boca Raton, FL, USA.
Bennett R.N, Wallsgrove R.M. 1994. Secondary Metabolites in Plant Defence Mechanisms. New Phytologist 127, 617–633.
Bieleski R.L. 1973. Phosphate Pools, Phosphate Transport, and Phosphate Availability. Annual Review of Plant Physiology 24, 225–252.
Bourgaud F.A.G, Miles S, Gontier E. 2001. Production of Plant Secondary Metabolites: a Historical Perspective. Plant Science 161, 839–851.
Briskin D.P. 2000. Medicinal Plants and Phytomedicines. Linking Plant Biochemistry and Physiology to Human Health. Plant Physiology 124, 507–514.
Chang C.C, Yang M.H, Wen H.M, Chern J.C. 2002. Estimation of Total Flavonoid Content in Propolis by Two Complementary Colorimetric Methods. Journal of Food and Drug Analysis 10, 178-182.
Davies K.M. 2008. Modifying Anthocyanin Production in Flowers. In: Anthocyanins. 49–80. Springer.
Estell R.E, Fredrickson L, James D.K. 2016. Effect of Light Intensity and Wavelength on Concentration of Plant Secondary Metabolites in the Leaves of Flourensia Cernua. Biochemical Systematics and Ecology 65, 108–114.
Ghasemzadeh A, Ghasemzadeh N. 2011. Flavonoids and Phenolic Acids: Role and Biochemical Activity in Plants and Humans. Journal of Medicinal Plants Research 5, 6697–6703.
Ghorbani N, Moradi H, Akbarpour V, Ghasemnezhad A. 2013. The Phytochemical Changes of Violet Flowers (Viola cornuta) Response to Exogenous Salicylic Acid Hormone. Journal of Chemical Health Risks 3(4), 01-08.
Ghormade V, Deshpande M.V, Paknikar K.M. 2011. Perspectives for Nano-biotechnology Enabled Protection and Nutrition of Plants. Biotechnology Advances 29, 792–803.
Glover B.J. 2007. Understanding Flowers and Flowering: An Integrated Approach. Vol. 277. Oxford University Press Oxford, UK.
Grabias B, Dombrowicz E, Kalemba D, Swiatek L. 1995. Phenolic Acids in Flores Bellidis and Herba Tropaeoli. Herba Polonica 41, 111–114.
Gudej J, Nazaruk J. 1997. Apigenin Glycosidoesters from Flowers of Bellis Perennis L. Acta Poloniae Pharmaceutica 54, 233–236.
Gudej J, Nazaruk J. 2001. Flavonol Glycosides from the Flowers of Bellis Perennis. Fitoterapia 72, 839–840.
Hapkins W.G. 1999. Introduction to Plant Physiology. Vol 1 and 2. John Wiley and Sons, New York.
Heyworth C.J, Iason G.R, Temperton V, Jarvis P.G, Duncan A.J. 1998. The Effect of Elevated CO2 Concentration and Nutrient Supply on Carbon-based Plant Secondary Metabolites in Pinus Sylvestris L. Oecologia 115, 344–350.
Iriti M, Rossoni M, Borgo M, Faoro F. 2004. Benzothiadiazole Enhances Resveratrol and Anthocyanin Biosynthesis in Grapevine, Meanwhile Improving Resistance to Botrytis Cinerea. Journal of
Ghorbani, Moradi, Kanani and Ashnavar Int. J. Hort. Sci. Technol. 2022 9(4): 405-414
413
Agricultural and Food Chemistry 52, 4406–4413.
Kalinova J, Dadakov, E. 2009. Rutin and Total Quercetin Content in Amaranth (Amaranthus spp.). Plant Foods for Human Nutrition 64, 68–74.
Kaur C, Kapoor H C. 2002. Antioxidant Activity and Total Phenolic Content of Some Asian Vegetables. International Journal of Food Science and Technology 37, 153–161.
Kim D.O, Jeong, S.W, Lee C.Y. 2003. Antioxidant Capacity of Phenolic Phytochemicals from Various Cultivars of Plums. Food Chemistry 81, 321–326.
Lal A, Ku M.S.B, Edwards G.E. 1996. Analysis of Inhibition of Photosynthesis Due to Water Stress in the C3 Species Hordeum Vulgare and Vicia Faba: Electron Transport. CO2 Fixation and Carboxylation Capacity. Photosynthesis Research 49, 57–69.
Luk’yanova L.D, Storozheva Z.I, Proshin A.T. 2007. Corrective Effect of Flavonoid-containing Preparation Extralife on the Development of Parkinson’s Syndrome.” Bulletin of Experimental Biology and Medicine 144, 42–45.
Marschner H. 2011. Marschner’s Mineral Nutrition of Higher Plants. Academic Press.
Meena MC, Patni V. 2008. Isolation and Identification of Flavonoid‘ Quercetin’ from Citrullus Colocynthis (Linn.) Schrad. Asian Journal of Experimental Sciences 22, 137–142.
Mogren L.M, Olsson M.E, Gertsson U.E. 2006. Quercetin Content in Field-cured Onions (Allium Cepa L.): Effects of Cultivar, Lifting Time, and Nitrogen Fertilizer Level. Journal of Agricultural and Food Chemistry 54, 6185–6191.
Mu C, Jia P, Yan Z, Liu X, Li X, Liu H. 2007. Quercetin Induces Cell Cycle G1 Arrest through Elevating Cdk Inhibitors P2l and P27 in Human Hepatoma Cell Line (HepG2). Methods and Findings in Experimental and Clinical Pharmacology 29, 179–184.
Nazaruk J, Gudej J. 2001. Qualitative and Quantitative Chromatographic Investigation of Flavonoids in Bellis Perennis L. Acta Poloniae Pharmaceutica 58, 401–405.
Nell M, Voetsch M, Vierheilig H, Steinkellner S, Zitterl-Eglseer K, Franz S, Novak J. 200). Effect of Phosphorus Uptake on Growth and Secondary Metabolites of Garden Sage (Salvia Officinalis L.). Journal of the Science of Food and Agriculture 89, 1090–1096.
Noack S.R, McBeat, T.M, McLaughlin M.J. 2010. Potential for Foliar Phosphorus Fertilisation of Dryland Cereal Crops: a Review. Crop and Pasture Science 61, 659–669.
Porra R.J. 2005. The Chequered History of the Development and Use of Simultaneous Equations for the Accurate Determination of Chlorophylls a and b. In: Discoveries in Photosynthesis 633–640. Springer.
Powell J.S, Raffa F. 1999. Sources of Variation in Concentration and Composition of Foliar Monoterpenes in Tamarack (Larix Laricina) Seedlings: Roles of Nutrient Availability, Time of Season, and Plant Architecture. Journal of Chemical Ecology 25, 1771–1797.
Rasmussen, Breinholt. 2003. Non-nutritive Bioactive Food Constituents of Plants: Bioavailability of Flavonoids. International Journal for Vitamin and Nutrition Research 73, 101–111.
Reich P.B, Oleksyn J, Wright I.J. 2009. Leaf Phosphorus Influences the Photosynthesis–nitrogen Relation: a Cross-biome Analysis of 314 Species. Oecologia 160, 207–212.
Robaszkiewicz A, Balcerczyk A, Bartosz G. 2007. Antioxidative and Prooxidative Effects of Quercetin on A549 Cells. Cell Biology International 31, 1245–1250.
Samee W, Vorarat S. 2007. Simultaneous Determination of Gallic Acid, Catechin, Rutin, Ellagic Acid, and Quercetin in Flower Extracts of Michelia Alba, Caesalpinia Pulcherrima and Nelumbo Nucifera by HPLC. Thai Pharmaceutical and Health Science Journal 2, 131–137.
Siatka T, Kašparová M. 2010. Seasonal Variation in Total Phenolic and Flavonoid Contents and DPPH Scavenging Activity of Bellis Perennis L. Flowers. Molecules 15, 9450–9461.
Soobrattee M.A, Neergheen V.S, Luximon-Ramma A, Aruoma O.I, Bahorun T. 2005. Phenolics as Potential Antioxidant Therapeutic Agents: Mechanism and Actions. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 579, 200–213.
Stewart A.J, Chapman W, Jenkins G.I, Graham I, Martin T, Crozier A. 2001. The Effect of Nitrogen and Phosphorus Deficiency on Flavonol Accumulation in Plant Tissues. Plant, Cell and Environment 24, 1189–1197.
Tilman D, Balzer C, Hill J, Befort B.L. 2011. Global Food Demand and the Sustainable Intensification of Agriculture. Proceedings of the National Academy of Sciences 108, 20260–20264.
Toki K, Saito N, Honda T. 1991. Three Cyanidin 3-glucuronylglucosides from Red Flowers of Bellis Perennis. Phytochemistry 30, 3769–3771.
Treutter D. 2006. Significance of Flavonoids in Plant Resistance: a Review. Environmental Chemistry Letters 4, 147–157.
Villano D, Fernández-Pachón M. S, Moyá M.L, Troncoso A M, García-Parrilla M C. 2007. Radical Scavenging Ability of Polyphenolic Compounds Towards DPPH Free Radical. Talanta 71, 230–235.
Wagner G.J. 1979. Content and Vacuole/extravacuole Distribution of Neutral Sugars, Free Amino Acids, and Anthocyanin in Protoplasts. Plant Physiology 64, 88–93.
Wink M. 1999. Functions of Plant Secondary Metabolites and Their Exploitation in Biotechnology. Vol. 3. Taylor and Francis.
Zima T, Fialová L, Mestek O, Janebová M, Crkovská J,
Ghorbani, Moradi, Kanani and Ashnavar Int. J. Hort. Sci. Technol. 2022 9(4): 405-414
414
Malbohan I, Štípek S, Mikulíková L, Popov P. 2001. Oxidative Stress, Metabolism of Ethanol and Alcohol-related Diseases. Journal of Biomedical Science 8, 59–70.