Document Type : Research paper


1 M. Sc. Horticultural Science Department, Agriculture and Natural Resources College, University of Hormozgan, Bandar Abbas, Iran

2 Department of Biotechnology Engineering, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran


Concerning highly restricted application of chemicals in postharvest technology of horticultural crops, it is necessary to introduce the safe methods for preserving food or methods of food preservation. This study aimed to improve quality and prolong storage life of strawberry fruit by application of grape seed and fruit skin extracts and to compare them with calcium chloride as a chemical. In this study, strawberry fruit was individually immersed in the 1% and 2% CaCl2 solutions, seed and skin extracts (1 and 2 mg L-1) and then placed in polyethylene packaging for 24 d at 5±1 °C. Measurements of firmness, titrable acid, pH, weight loss, total antioxidant capacity, total phenolic, anthocyanin, vitamin C, enzymes' activity including catalase, peroxidase, and polyphenol oxidase and decay were carried out at 0, 6, 12, 18, and 24 d of storage. All applied treatments caused a significant effect on measured parameters including weight loss, titratable acidity, decay percentage and firmness, maintenance of anthocyanin and vitamin C contents, total phenolic, and antioxidant capacity. However, grape skin extract and grape seed extract showed the best results. Therefore, it can be concluded that Shiraz dark grape seed and skin extracts have the potential to control the decay incidence, prolong the storage life and preserve of postharvest valuable attributes of strawberry. 


Alexandratos N, Bruinsma J. 2012. “World agriculture towards 2030/2050: the saving water. From Field to Fork-Curbing Losses and Wastage in the Food Chain 2012 revision.” Working paper: FAO: ESA No. 12-03, p.4.
A.O.A.C. 2000. Vitamins and other nutrients (Chapter 45). In Official Methods of Analysis (17th ed.), Washington, D.C., pp: 16-20.
Barbosa L.N, Rall V.L, Fernandes A.A, Ushimaru P.I, da Silva Probst I, Fernandes A. 2009. Essential oils against foodborne pathogens and spoilage bacteria in minced meat. Foodborne Pathogens and Disease 6 (6), 725-728.
Ballester A.R, Lafuente M.T, González-Candelas L. 2006. Spatial study ofantioxidant enzymes, peroxidase and phenylalanine ammonia-lyase in thecitrus fruit-Penicillium digitatum interaction. Postharvest Biology and Technology 39,115–124.
Brand-Williams W, Cuvelier M.E, Berset C.L.W.T. 1995. Use of a free radical method to evaluate antioxidant activity. LWT-Food Science and Technology 28 (1), 25-30.‏
Canuto G.A.B, Oliveira D.R, Da Conceição L.S.M, Farah J.P.S, Tavares M.F.M. 2016. Development and validation of a liquid chromatography method for anthocyanins in strawberry (Fragaria spp.) and complementary studies on stability, kinetics and antioxidant power. Food Chemistry 192, 566–574.
Cao S, Zheng Y, Yang Z, Tang S, Jin P, Wang K, Wang X. 2008. Effect of methyl jasmonate on inhibition of Colletotrichum acutatum infection in loquat fruit and the possible echanisms. Postharvest Biology and Technology 49, 301–307.
ChanceB, Maehly C.1955. Assay of catalase and peroxidases. Methods in Enzymology 11, 764-775.
Chun O.K, Kim D.O, Lee C.Y. 2003. Superoxide radical scavenging activity of the major polyphenols in fresh plums. Journal of Agricultural and Food Chemistry 51 (27), 8067-8072.‏
Cisneros-Zevallos, L., 2006. The use of controlled postharvest abiotic stresses as a tool for enhancing nutraceutical content and adding value of fresh fruit and vegetables. Journal of Food Science 68 (5), 1560-1565.
Delmas  D. 2013. Resveratrol: Sources, Production and Health Benefits; Nova Science Publishers Inc.: Hauppauge, NY, USA. Pp. 275-296.
El Ghaouth A, Arul J, Ponnampalam R, Boulet M. 1991. Chitosan coating effect on stability and quality of fresh strawberries. Journal of Food Science 56 (6), 1618–1620.
FAO, 2019. Seeking end to loss and waste of food along production chain. Food and Agriculture Organization (FAO) of the United Nations. .
Feng W, Zheng X. 2007. Essential oils to control Alternaria alternata in vitro and in vivo. Food Control 18, 1126–1130.
Fiume M.M, Bergfeld W.F, Belsito D.V, Hill R.A, Klaassen C.D, Liebler D.C, Marks J.G, Shank R.C, Slaga T.J, Snyder P.W, Andersen F.A. 2014. Safety assessment of Vitis vinifera (Grape)-derived ingredients as used in cosmetics. International Journal of Toxicology 33, 48–83.
Gebel M.P, Magurno F. 2014. Assessment of the antifungal potential of the essential oil from Thymus vulgaris against Botrytis cinerea causative agent of postharvest gray mold on strawberry fruit. Journal of Agricultural and Environmental Science 1 (2). 17-24.
Han C, Zhao Y, Leonard S.W, Traber M.G. 2004. Edible coatings to improve storability and enhance nutritional value of fresh and frozen strawberries (Fragaria × ananassa) and raspberries (Rubus ideaus). Postharvest Biology and Technology 33, 67-78.
Hernandez-Munoz P, Almenar E, Del Valle V, Velez D, Gavara R. 2008. Effect of chitosan coating combined with postharvest calcium treatment on strawberry (Fragaria× ananassa) quality during refrigerated storage. Food Chemistry 110 (2), 428-435.‏
Hong K, Xie J, Zhang L, Sun D, Gong D. 2012. Effects of chitosan coating on postharvest life and quality of guava (Psidium guajava L.) fruit during cold storage. Scientia Horticulturae 144, 172-178.
Jin P, Zheng Y, Tang S, Rui H, Wang C.Y. 2009. A combination of hot air and methyl jasmonate vapor treatment alleviates chilling injury of peach fruit. Postharvest Biology and Technology 52, 24–29.
Kar M, Mishra D. 1976. Catalase, peroxidase, and polyphenoloxidase activities during rice leaf senescence. Plant Physiology 57, 315-319.
Kumah P, Olympio N.S, Tayviah C.S. 2011.  Sensitivity of three tomato (Lycopersicon esculentum) cultivars - Akoma, Pectomech and power- to chilling injury. Agriculture and Biology Journal of North America 2 (5), 799-805.
Martinez-Romero D, Castillo S, Valverde J.M, Gullen F, Valero D, Serrano M. 2005. The use of natural aromatic essential oils helps to maintain postharvest quality of Crimson table grapes. Acta Horticulturae 682, 1723–1729.
Martinez-Ferrer M, Harper C, Perez-Muroz F, Chaparro M. 2002. Modified atmosphere packaging of minimally processed mango and pineapple fruit. Journal of Food Science 67, 3365-3371.
Martinez-Romero D, Guillen F, Valverde J.M, Bailen G, Zapata P.J, Serrano M. 2007. Influence of carvacrol on survival of Botrytis cinerea inoculated in table grapes. International Journal of Food Microbiology 115, 144–148. doi: 10.1016/j.ijfoodmicro.2006.10.015.
Mazumda B.C, Majumder K. 2003. Methods on physicochemical analysis of fruit. Uni. College of Agric. Calcutta University. pp. 108- 109.
Mittler, R., 2002. Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science 7, 405–410.
Munin A, Edwards-Lévy F. 2011. Encapsulation of natural polyphenolic compounds: A review. Pharmaceutics 3, 793–829.
Oms-Oliu G, Rojas-Grau M. A, Alandes G. L, Varela P, Soliva- Fortuny R, Hernando H.M.I, Perez Munuera I, Fiszman S, Martin-Belloso O. 2010. Recent approaches using chemical treatments to preserve quality of fresh-cut fruit: a review. Postharvest Biology and Technology 57, 139–178.
Perkins-Veazie P. 1995. Growth and ripening of strawberry fruit. Horticultural Reviews 17, 267–297.
Pezzuto J.M, Kondratyuk T.P, Ogas T. 2013. Resveratrol derivatives: A patent review (2009–2012). Expert Opinion on Therapeutic Patents 23, 1529–1546.
Rattanapitigorn P, Arakawa M, Tsuro M. 2006. Vanillin enhances the antifungal effect of plant essential oils against Botrytis cinerea. International Journal of Aromatherapy 16, 193–198.
Serrano M, Martinez-Romero D, Castillo S, Guillen F, Valero D. 2005. The use of the natural antifungal compounds improves the beneficial effect of MAP in sweet cherry storage. Innovative Food Science Emerging Technologies 6, 115–123.
Skrovankova S, Sumczynski D, Mlcek J, Jurikova,T, Sochor J. 2015. Bioactive compounds and antioxidant activity in different types of berries. International Journal of .Molecular Science 16, 24673–24706.
Slinkard K, Singleton V.L. 1977. Total phenol analyses: Automation and Comparison with Manual Methods. American Journal of Enology and Viticulture 28, 49-55.
Sogvar O.B, Koushesh Saba M, Emamifar A. 2016. Aloe vera and ascorbic acid coatings maintain postharvest quality and reduce microbial load of strawberry fruit. Postharvest Biology and Technology 114, 29–35.
Soto-Zamora G, Yahia E.M, Brecht J.K, Gardea A. 2005. Effects of postharvest hot air treatments on the quality and antioxidant levels in tomato fruit. LWT-Food Science and Technology 38 (6), 657-663.
Tzortzakis N.G. 2007. Maintaining postharvest quality of fresh produce with volatile compounds. Innovative Food Science Emerging Technologies 8, 111–116. doi: 10.1016/j.ifset.2006.08.001.
Valero D, Valverde J.M, Martinez-Romero D, Guillen F, Castillo S, Serrano M. 2006. The combination of modified atmosphere packaging with eugenol or thymol to maintain quality, safety and functional properties of table grapes. Postharvest Biology and Technology 41, 317–327.
Vicente A.R, Martínez G.A, Civello P.M, Chaves A.R. 2002. Quality ofheat-treated strawberry fruit during refrigerated storage. Postharvest Biology and Technology 25, 59–71.
Wada L, Ou B. 2002. Antioxidant activity and phenolic content of Oregon caneberries. Journal of Agricultural and Food Chemistry 50 (12), 3495-3500.‏
Wagner G.J. 1979. Content and vacuole/extravacuole distribution of neutral sugars, free amino acids, and anthocyanin in protoplasts. Plant Physiology 64 (1), 88-93.‏
Wang S.Y, Gao H. 2012. Effect of chitosan-based edible coating on antioxidants, antioxidant enzyme system, and postharvest fruit quality of strawberries (Fragaria x aranassa Duch). Food Science and Technology 52 (6), 71–79.
Xiao Z, Luo Y, Luo Y, Wang Q. 2011. Combined effects of sodium chlorite dip treatment and chitosan coatings on the quality of fresh-cut d’Anjou pears. Postharvest Biology and Technology 62, 319–326.
Xing Y, Li X, Xu Q, Jiang Y, Yun J, Li W. 2010. Effects of chitosan-based coating and modified atmosphere packaging (MAP) on browning and shelf life of fresh-cut lotus root (Nelumbo nucifera Gaerth). Innovative Food Science Emerging Technologies 4, 684–689.
Xing Y, Li X, Yun J, Lu Y. 2011. Extending the shelf-life of fresh-cut lotus root with antibrowning agents, cinnamon oil fumigation, and moderate vacuum packaging (MVP). Journal of Food Process Engineering 35, 505–521.
Yao H.J, Tian S.P. 2005. Effects of a biocontrol agent and methyl jasmonate on postharvest disease of peach fruit and the possible mechanism involved. Journal of Applied Microbiology 9, 941–950.
Yu-Jie Z Ren-You G, Sha L, Yue Z, An-Na L, Dong-Ping X, Hua-Bin L, 2015. Antioxidant phytochemicals for the prevention and treatment of chronic diseases. Molecules 20, 21138–21156;
Zamani-Zadeh M, Soleimanian-Zad S, Sheikh-
Zeinoddin M, Goli S.A.H, 2014. Integration of Lactobacillus plantarum A7 with thyme and cumin essential oils as a potential biocontrol tool for gray mold rot on strawberry fruit. Postharvest Biology and Technology 92. 149-156.
Zeng K.F, Deng, Y.Y, Ming, J, Deng, L.L, 2010. Induction of disease resistance and ROS metabolism in navel oranges by chitosan. Scientia Horticulturae 126, 223–228.