Document Type : Research paper

Authors

1 Kenya agricultural and livestock research organization

2 Department of Plant, Animal and Food Science, Jaramogi Oginga Odinga University of Science and Technology

Abstract

Leaf spot (Cercospora moricola, Cooke) is a disease that negatively influences the yield of mulberry (Morus alba L.) plants. To determine the effect of nitrogen levels on the incidence and severity of leaf spot an experiment was carried out on mulberry plants. The nitrogen levels included 0, 100, 200 and 300 kg ha-1, which were applied in two splits coinciding with the two rainy seasons. The study design was randomized complete block design (RCBD) with three replications. Determination of disease intensity involved scoring for disease intensity on a 1-5 Manandhar scale and calculation of the disease incidence were performed by expressing the number of infected leaves as a percentage of the total number of leaves. The values were translated to area under disease progress stairs (AUDPS). The means for AUDPS were subjected to analysis of variance (ANOVA) using PRO GLM in SAS and Fisher’s least significant difference (LSD) used to partition the means at p≤0.05. The results showed that as the rate of nitrogen application was increased, there was a corresponding decrease in AUDPS for disease incidence and a decrease in AUDPS for disease severity. From the obtained results it can be concluded that nitrogen at an application rate of 200 kg ha-1 is an effective approach to suppress Cercospora leaf spot of mulberry and can be recommended to the farmers, where this disease is a problem for cultivation of mulberry.

Keywords

Adolkar V.V, Raina S.K, Kimbu D.M. 2007. Evaluation of various Mulberry Morus spp. (Moraceae) cultivars for the rearing of the bivoltine hybrid race Shaashi BV-333 of the silkworm Bombix mori (Lepidoptera: Bombicidae). Tropical insect Science 27, 6-14.
Ahmed F, Kader M.A, Sultana R, Ahmed O, Begun S.A, Iqbal M.F. 2019. Combined application of foliar fertilizer with basal NPK enhances mulberry leaf yield and silkworm cocoon productivity in calcareous soil. Pakistan Journal of biological science 2, 1002-1005.
Chiang K.S, Liu H.J, Bock, C.H. 2017. A discussion on disease severity index values. Part 1n: warning on inherent errors and suggestions to maximize accuracy. Annals of Applied Biology, 2, 1-2.
Daub M.E, Ehrenshaft M. 2000. The Photo activated Cercospora toxin cercosporin: Contributions to plant disease and fundamental biology. Annual Reviews in Phytopathology 38, 461-490. 
Dordas C. 2008. Role of nutrients on controlling plant diseases in sustainable agriculture. A review. Agronomy for sustainable development 28, 33-46.
Gathumbi M.N. 2008. The effect of fertilizers and mulberry (M. alba L) variety on cocoon and silk quality in Kenya. MSc thesis, Nairobi University.
Ghoes L, Neela F.A, Chakravorty T.C, Ali M.R, Alam M.S. 2010. Incidence of leaf blight disease of mulberry plant and assessment of changes in amino acids and photosynthetic pigments of infected leaf. Plant pathology Journal 9, 3.
Ghoshi L, Neela F.A, Mahal M.F, Khatum M.J, Ali M.R.  2012. Effect of various factors on the development of leaf spot disease in mulberry. Journal of environmental science and natural resources 5, 205-209.
Huber D.M, Haneklaus S. 2007. Managing nutrients to control plant diseases. Landbauforsch Volkenrode 57, 313-322.
Huber D, Römheld V, Weinmann M. 2012. Relationship between nutrition, plant diseases and pests. In Marschner's mineral nutrition of higher plants 2012 Jan 1 (pp. 283-298). Academic Press.
Kone N, Asare-Bediako E, Silue S, Kone D, Koita O, Menze, W, Winter S. 2017. Influence of planting date on incidence and severity of viral disease on cucurbits under field conditions. Annals of Agriculture, 62, 1-2. 
Kuribayashi S. 1986. Department of sericulture, Sericultural Experiment station, Tsukuba Ibaraki 305, Japan.
Manandhar H.K, Timila R.D, Sharma S, Joshi S, Sthapit B.R. 2016. A field guide for identification and scoring methods of diseases in the mountain crops of Nepal. Bioversity International, 1, 1-11.
Mitchell C.E, Reich P.B, Tilman D, Groth J.V. 2003. Effects of elevated CO2 Nitrogen deposition and decreased species diversity on foliar plant diseases. Global change biology. 9, 438-451.
Nderitu W.P, Ngode, Kinyua G.M, Mutui M.T. 2012. Field evaluation of mulberry accessions for susceptibility to foliar diseases in Uasin Gishu district, Kenya. African Journal of Biotechnology11, 3569-3574.
Parthasarathy S. 2015. Effect of fertilizers on plant diseases. PhD thesis, Tamil Nadu agricultural university, Coimbatore 641003, Tamilnadu.
Ray P, Martin H.P. 2012. Impact of feeding Neochetina weevils on pathogenicity of fungi associated with water hyacinth in South Africa. Journal of Aquatic Plant Management, 50, 79-84.
Reddy R.C.G, Nirmala R.S, Ramanamma C.H. 2009. Efficacy of phytoextracts and oils of certain medicinal plants against C. moricola Cook, incidence of mulberry leaf spot. Journal of biopesticides 2, 77-83.
Sajad U.H, Hasan S.S, Dhar A.V. 2014. Nutrigenic Efficiency Change and Cocoon Crop Loss due to Assimilation of Leaf Spot Diseased Mulberry Leaf in Silkworm, Bombyx mori L... Journal of Plant Pathology and Microbiology 5, 220.
Sexton A.C, Howlett B.J. 2006. Parallels in fungal pathogenesis onplant and animal hosts. Eukaryotic cell. 5, 1941-1949.
SimkoI, Piepho H.P. 2012. The Area under Disease Progress Stairs: Calculation, Advantage and Application. Phytopathology Journal. 102, 2012-381.
Singh B.K, Singh T. 2010. Muga silkworm seed organization, P-4 unit Mendipathar, East Garo Hills, Meghalaya.
Sugiyama M, Takahashi M, Katsube T, Koyama A, Itamura H. 2016. Effects of applied Nitrogen on the amounts of the functional components of mulberry (M. alba L) leaves. Journal of Agriculture and food chemistry 37, 6923-6929.
To-Anun C, Hidayat I, Meeboon J. 2011. Genus Cercospora in Thailand: Taxonomy and phylogeny (with a dichotomous key to species). Journal of plant pathology and quarantine, 1, 11-87.
Tuikong D.R, Kipkurgat T.K, Madera D.S. 2015. Mulberry and silk production in Kenya. Journal of Textile science and Engineering, 5, 2-7.
Veresoglou S.D, Barto E.K, Menexes G, Rillig M.C.
2012. Fertilization effects on severity of disease caused by fungal plant pathogens. Plant pathology journal 62, 961-969.
Walters D.R, Bingham I.J. 2007. Influence of nutrition on disease development caused by fungal pathogens: implications for plant disease control. Annals of applied biology 151, 307-324.