Document Type : Research paper
Authors
Flower Research Institute of Yunnan Academy of Agricultural Sciences, Kunming, Yunnan, 650205, China National Engineering Research Center for Ornamental Horticulture, Kunming, Yunman, 650205, China
Abstract
Roses are cultivated as ornamental plants in gardens, cut flowers and potted flowers, but also serviced in the perfume industry. Rose flowers product different volatile compounds including eugenol, being floral attractant for pollinators and used as an important composition of essential oil. Here, the eugenol synthase gene (RcEGS1) promoter was cloned from R. chinensis 'Old Blush'. The sequences of RcEGS1 promoter were 1776bp, contained the conserved elements, and predicted to be with several cis-regulatory elements involved in secondary metabolism of plants. Yeast one-hybrid library of R. chinensis 'Old Blush' at flower opening was constructed with the three-frame reading. The library capacity was more than 1.12×107CFU. The length of fragments inserted was 1 Kb to 3 Kb, which is fully meeting the demand of selecting the yeast library. The bait yeast vectors with cis-transcription elements MBS-box, G-box and one fragment bait yeast vectors were constructed, respectively. Furthermore, the transcript factors R2R3-type MYB and bHLH3 were screened using the yeast one-hybrid system. The results suggested the two TFs might play an important role in the pathway of volatile biosynthesis in roses.
Keywords
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Hui-jun Yan et al. Int. J. Hort. Sci. Technol. 2021 8(3): 215-225
224
using expressed sequence tags. FEBS Letters 515: 35-38.
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6. Grossman J. 1993. Botanical pesticides in Africa. International Journal of Pest Managment. Pract 15:1-9.
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11. Lin-Wang K, Bolitho K, Grafton K, Kortstee A, Karunairetnam S, McGhie T.K, Espley R.V, Hellens R.P, Allan A.C. 2010. An R2R3 MYB transcription factor associated with regulation of
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12. Luo Y, Vijaychander S, Stile J, Zhu L. 1996. Cloning and analysis of DNA-binding proteins by yeast one-hybrid and one-two-hybrid systems. Biotechniques 20(4): 564-568.
13. Magnard J.L, Roccia A, Caissard J.C, Vergne P, Sun P, Hecquet R, Dubois A, Hibrand-Saint Oyant L, Jullien F, Nicolè F, Raymond O, Huguet S, Baltenweck R, Meyer S, Claudel P, Jeauffre J, Rohmer M, Foucher F, Hugueney P, Bendahmane M, Baudino S. 2015. Biosynthesis of monoterpene scent compounds in roses. Science 349(6243): 81-83.
14. Menkens A.E, Schindler U, Cashmore A.R. 1995. The G-box: a ubiquitous regulatory DNA element in plants bound by the GBF family of bZIP proteins. Trends in Biochemical Sciences 20: 506-510.
15. Ohara O, Temple G. 2001. Directional cDNA library construction assisted by the in vitro recombination reaction. Nucleic Acid Research 29(4):e22.
16. Quattrocchio F, Wing J.F, van der Woude K, Mol J.N, Koes R. 1998. Analysis of bHLH and MYB-domain proteins: Species-specific regulatory differences are caused by divergent evolution of target anthocyanin genes. Plant Journal 13: 475-488.
17. Raymond O, Gouzy J, Just J, Badouin H, Verdenaud M, Lemainque A,Vergne P, Moja S, Choisne N, Pont C, et al. 2018. The Rosa genome provides new insights into the domestication of modern roses. Nature Genetics 50: 772-777.
18. Reece-Hoyes J.S, Pons C, Diallo A, Mori A, Shrestha S, Kadreppa S, Nelson J, Diprima S, Dricot A, Lajoie B.R, et al. 2013. Extensive rewiring and complex evolutionary dynamics in a C. elegans multiparameter transcription factor network. Molecular Cell 51:116-127.
19. Roccia A, Hibrand-Saint Oyant L, Cavel E, Caissard J.C, Machenaud J, Thouroude T, Jeauffre J, Bony A, Dubois A, Vergne P, Szécsi J, Foucher F, Bendahmane M, Baudino S. 2019. Biosynthesis of 2-Phenylethanol in Rose Petals Is Linked to the Expression of One Allele of RhPAAS. Plant Physiology 179: 1064-79.
20. Rusanov K.E, Kovacheva N.M, Atanassov I.I. 2011. Comparative GC/MS analysis of rose flower and distilled oil Volatiles of the oil bearing rose Rosa damascene. Biotechnology & Biotechnological Equipment 25(1): 2210-2216.
Hui-jun Yan et al. Int. J. Hort. Sci. Technol. 2021 8(3): 215-225
225
21. Scalliet G, Lionnet C, Le Bechec M, Dutron L, Magnard J.L, Baudino S, Bergougnoux V, Jullien F, Chambrier P, Vergne P, Dumas C, Cock J.M, Hugueney P. 2006. Role of petal-specific orcinol O-methyltransferases in the evolution of rose scent. Plant Physiology 140(1): 18-29.
22. Scalliet G, Piola F, Douady C.J, Réty S, Raymond O, Baudino S, Bordji K, Bendahmane M, Dumas C, Cock J.M. 2008. Hugueney P. Scent evolution in Chinese roses. Proceedings of the National Academy of Sciences of USA 105 (15), 5927-5932.
23. Schaart J.G, Dubos C, Romero D.L.I, van H, Vos R.C, Jonker H.H, Xu W, Routaboul J.M, Lepiniec L, Bovy A.G. 2013. Identification and characterization of MYB-bHLH-WD40 regulatory complexes controlling proanthocyanidin biosynthesis in strawberry (Fragaria × ananassa) fruits. New Phytologist 197: 454-467.
24. Shalit M, Guterman I, Volpin H, Bar E, Tamari T, Menda N, Adam Z, Zamir D, Vainstein A, Weiss D, Pichersky E, Lewinsohn E. 2003. Volatileester formation in roses: Identification of an acetyl-CoA: Geraniol/Citronellol acetyltransferase in developing rose petals. Plant Physiology 131(4): 1868-1876.
25. Spitzer-Rimon B, Farhi M, Albo B, Cna'ani A, Ben Zvi M.M, Masci T, Edelbaum O, Yu Y, Shklarman E, Ovadis M, Vainstein A. 2012. The R2R3-MYB-like regulatory factor EOBI, acting downstream of EOBII, regulates scent production by activating ODO1 and structural scent-related genes in petunia. Plant Cell 24(12): 5089-5105.
26. Spitzer-Rimon B, Marhevka E, Barkai O, Marton I, Edelbaum O, Masci T, Prathapani, NK, Shklarman E, Ovadis M, Vainstein A. 2010. EOBП, a gene encoding a flower-specific regulator of phenylpropanoid volatiles’ biosynthesis in petunia. Plant Cell 22(6): 1961-1976.
27. Sun P, Schuurink R.C, Caissard J.C, Hugueney P,
Baudino S. 2016. My Way: Noncanonical Biosynthesis Pathways for Plant Volatiles. Trends in Plant Science 21(10): 884-894.
28. Verdonk J.C, Haring M.A, van Tunen A.J, Schuurink R.C. 2005. ODORANT1 regulates fragrance biosynthesis in petunia flowers. Plant Cell 17(5): 1612-1624.
29. Wang H.P, Yan H.J, Zhang H, Jian H.Y, Wang Q.G, Qiu X.Q, Li S.B, Zhou N.N, Tang K.X. 2012. Cloning and Expression Analysis of Eugenol Synthase Gene RcEGS1 in Rosa chinensis 'Pallida'. Acta Horticulturae Sinica 39: 1387-1394.
30. Weiss E.A. 1997. Rosaceae. In: Weiss, E.A. (Ed.), Essential Oil Crops. CAB International Wallingford, Oxon, UK, pp. 393-416.
31. Yan H.J, Baudino S, Caissard J.C, Nicolè F Zhang H, Tang K.X, Li S.B, Lu S.G. 2018. Functional characterization of the eugenol synthase gene (RcEGS1) in rose. Plant Physiology and Biochemistry 129: 21-26.
32. Yan H.J, Yang J.K, Zhang H, Li S.B, Zhang T, Wang J.H, Zhou N.N, Chen M, Tang K.X. 2015. Use of digital gene expression to discriminate gene expression in different developmental stages of Rosa chinenssis ‘Pallida’. Acta Horticulturae 1064: 115-121.
33. Yan H.J, Zhang H, Wang Q.G, Jian H.Y, Xiu X.Q, Wang J.H, Tang K.X. 2011. Isolation and identification of a putative scent-related gene RhMYB1 from rose. Molecular Biology Reports 38, 4475-4482.
34. Yang H, Zhou Y, Zhang Y, Wang J, Shi H. 2019. Identification of transcription factors of nitrate reductase gene promoters and NRE2 cis-element through yeast one-hybrid screening in Nicotiana tabacum. BMC Plant Biology 19(1):145.
35. Zvi M.M, Shklarman E, Masci T, Kalev H, Debener T, Shafir S, Ovadis M, Vainstein A. 2012. PAP1 transcription factor enhances production of phenylpropanoid and terpenoid scent compounds in rose flowers. New Phytologist 195(2): 335-345.
2. Channelière S, Rivièrea S, Scallieta G, Szecsia J, Jullienc F, Dollea C, Vergneab P, Dumasab C, Bendahmanea M, Hugueneya P, Cocka J.M. 2002. Analysis of gene expression in rose petals
Hui-jun Yan et al. Int. J. Hort. Sci. Technol. 2021 8(3): 215-225
224
using expressed sequence tags. FEBS Letters 515: 35-38.
3. Colquhoun T.A, Marciniak D.M, Wedde A.E, Kim J.Y, Schwieterman M.L, Levin L.A, Van Moerkercke A, Schuurink R.C, Clark D.G. 2012. A peroxisomally localized acylactivating enzyme is required for volatile benzenoid formation in a Petunia×hybrida cv. 'Mitchell Diploid' flower. Journal of Experimental Botany 63(13): 4821-33.
4. Deplancke B, Mukhopadhyay A, Ao W, Elewa A.M, Grove C.A, Martinez N.J, Sequerra R, Doucetta-Stamm L, Reece-Hoyce J.S, Hope I.A, Tissenbaum H.A, Mango S.E, Walhout A.J.M. 2006. A gene-centered C. elegans protein-DNA interaction network. Cell 125:1193-1205.
5. Dubois A, Carrere S, Raymond O, Pouvreau B, Cottret L, Roccia A, Onesto J.P, Sakr S, Atanassova R, Baudino S, Foucher F, Bris M.L, Gouzy J. 2012. Bendahmane M. Transcriptome database resource and gene expression atlas for the rose. BMC Genomics 13 (1): 638.
6. Grossman J. 1993. Botanical pesticides in Africa. International Journal of Pest Managment. Pract 15:1-9.
7. Han Y, Wan H, Cheng T, Wang J, Yang W, Pan H, Zhang Q. 2017. Comparative RNA-seq analysis of transcriptome dynamics during petal development in Rosa chinensis. Scientific Reports 7, 43382.
8. Hibrand-Saint Oyant L, Ruttink T, Hamama L, Kirov I, Lakhwani D, Zhou N.N, et al 2018. A high-quality genome sequence of Rosa chinensis to elucidate ornamental traits. Nature plants 4(7):473-484.
9. Koeduka T, Louie G.V, Orlova I, Kish C.M, Ibdah M, Wilkerson C.G, Bowman M.E, Baiga T.J, Noel J.P, Dudareva N, Pichersky E. 2008. The multiple phenylpropene synthases in both Clarkia breweriand Petunia hybrid represent two distinct protein lineages. Plant Journal 54(3): 362-374.
10. Kozioł A, Stryjewska A, Librowski T, Salat K, Gawel M, Moniczewski A, Lochyński S. 2014. An overview of the pharmacological properties and potential applications of natural monoterpenes. Mini-Reviews in Medicinal Chemistry 14, 1156-1168.
11. Lin-Wang K, Bolitho K, Grafton K, Kortstee A, Karunairetnam S, McGhie T.K, Espley R.V, Hellens R.P, Allan A.C. 2010. An R2R3 MYB transcription factor associated with regulation of
the anthocyanin biosynthetic pathway in Rosaceae. BMC Plant Biology 10: 50.
12. Luo Y, Vijaychander S, Stile J, Zhu L. 1996. Cloning and analysis of DNA-binding proteins by yeast one-hybrid and one-two-hybrid systems. Biotechniques 20(4): 564-568.
13. Magnard J.L, Roccia A, Caissard J.C, Vergne P, Sun P, Hecquet R, Dubois A, Hibrand-Saint Oyant L, Jullien F, Nicolè F, Raymond O, Huguet S, Baltenweck R, Meyer S, Claudel P, Jeauffre J, Rohmer M, Foucher F, Hugueney P, Bendahmane M, Baudino S. 2015. Biosynthesis of monoterpene scent compounds in roses. Science 349(6243): 81-83.
14. Menkens A.E, Schindler U, Cashmore A.R. 1995. The G-box: a ubiquitous regulatory DNA element in plants bound by the GBF family of bZIP proteins. Trends in Biochemical Sciences 20: 506-510.
15. Ohara O, Temple G. 2001. Directional cDNA library construction assisted by the in vitro recombination reaction. Nucleic Acid Research 29(4):e22.
16. Quattrocchio F, Wing J.F, van der Woude K, Mol J.N, Koes R. 1998. Analysis of bHLH and MYB-domain proteins: Species-specific regulatory differences are caused by divergent evolution of target anthocyanin genes. Plant Journal 13: 475-488.
17. Raymond O, Gouzy J, Just J, Badouin H, Verdenaud M, Lemainque A,Vergne P, Moja S, Choisne N, Pont C, et al. 2018. The Rosa genome provides new insights into the domestication of modern roses. Nature Genetics 50: 772-777.
18. Reece-Hoyes J.S, Pons C, Diallo A, Mori A, Shrestha S, Kadreppa S, Nelson J, Diprima S, Dricot A, Lajoie B.R, et al. 2013. Extensive rewiring and complex evolutionary dynamics in a C. elegans multiparameter transcription factor network. Molecular Cell 51:116-127.
19. Roccia A, Hibrand-Saint Oyant L, Cavel E, Caissard J.C, Machenaud J, Thouroude T, Jeauffre J, Bony A, Dubois A, Vergne P, Szécsi J, Foucher F, Bendahmane M, Baudino S. 2019. Biosynthesis of 2-Phenylethanol in Rose Petals Is Linked to the Expression of One Allele of RhPAAS. Plant Physiology 179: 1064-79.
20. Rusanov K.E, Kovacheva N.M, Atanassov I.I. 2011. Comparative GC/MS analysis of rose flower and distilled oil Volatiles of the oil bearing rose Rosa damascene. Biotechnology & Biotechnological Equipment 25(1): 2210-2216.
Hui-jun Yan et al. Int. J. Hort. Sci. Technol. 2021 8(3): 215-225
225
21. Scalliet G, Lionnet C, Le Bechec M, Dutron L, Magnard J.L, Baudino S, Bergougnoux V, Jullien F, Chambrier P, Vergne P, Dumas C, Cock J.M, Hugueney P. 2006. Role of petal-specific orcinol O-methyltransferases in the evolution of rose scent. Plant Physiology 140(1): 18-29.
22. Scalliet G, Piola F, Douady C.J, Réty S, Raymond O, Baudino S, Bordji K, Bendahmane M, Dumas C, Cock J.M. 2008. Hugueney P. Scent evolution in Chinese roses. Proceedings of the National Academy of Sciences of USA 105 (15), 5927-5932.
23. Schaart J.G, Dubos C, Romero D.L.I, van H, Vos R.C, Jonker H.H, Xu W, Routaboul J.M, Lepiniec L, Bovy A.G. 2013. Identification and characterization of MYB-bHLH-WD40 regulatory complexes controlling proanthocyanidin biosynthesis in strawberry (Fragaria × ananassa) fruits. New Phytologist 197: 454-467.
24. Shalit M, Guterman I, Volpin H, Bar E, Tamari T, Menda N, Adam Z, Zamir D, Vainstein A, Weiss D, Pichersky E, Lewinsohn E. 2003. Volatileester formation in roses: Identification of an acetyl-CoA: Geraniol/Citronellol acetyltransferase in developing rose petals. Plant Physiology 131(4): 1868-1876.
25. Spitzer-Rimon B, Farhi M, Albo B, Cna'ani A, Ben Zvi M.M, Masci T, Edelbaum O, Yu Y, Shklarman E, Ovadis M, Vainstein A. 2012. The R2R3-MYB-like regulatory factor EOBI, acting downstream of EOBII, regulates scent production by activating ODO1 and structural scent-related genes in petunia. Plant Cell 24(12): 5089-5105.
26. Spitzer-Rimon B, Marhevka E, Barkai O, Marton I, Edelbaum O, Masci T, Prathapani, NK, Shklarman E, Ovadis M, Vainstein A. 2010. EOBП, a gene encoding a flower-specific regulator of phenylpropanoid volatiles’ biosynthesis in petunia. Plant Cell 22(6): 1961-1976.
27. Sun P, Schuurink R.C, Caissard J.C, Hugueney P,
Baudino S. 2016. My Way: Noncanonical Biosynthesis Pathways for Plant Volatiles. Trends in Plant Science 21(10): 884-894.
28. Verdonk J.C, Haring M.A, van Tunen A.J, Schuurink R.C. 2005. ODORANT1 regulates fragrance biosynthesis in petunia flowers. Plant Cell 17(5): 1612-1624.
29. Wang H.P, Yan H.J, Zhang H, Jian H.Y, Wang Q.G, Qiu X.Q, Li S.B, Zhou N.N, Tang K.X. 2012. Cloning and Expression Analysis of Eugenol Synthase Gene RcEGS1 in Rosa chinensis 'Pallida'. Acta Horticulturae Sinica 39: 1387-1394.
30. Weiss E.A. 1997. Rosaceae. In: Weiss, E.A. (Ed.), Essential Oil Crops. CAB International Wallingford, Oxon, UK, pp. 393-416.
31. Yan H.J, Baudino S, Caissard J.C, Nicolè F Zhang H, Tang K.X, Li S.B, Lu S.G. 2018. Functional characterization of the eugenol synthase gene (RcEGS1) in rose. Plant Physiology and Biochemistry 129: 21-26.
32. Yan H.J, Yang J.K, Zhang H, Li S.B, Zhang T, Wang J.H, Zhou N.N, Chen M, Tang K.X. 2015. Use of digital gene expression to discriminate gene expression in different developmental stages of Rosa chinenssis ‘Pallida’. Acta Horticulturae 1064: 115-121.
33. Yan H.J, Zhang H, Wang Q.G, Jian H.Y, Xiu X.Q, Wang J.H, Tang K.X. 2011. Isolation and identification of a putative scent-related gene RhMYB1 from rose. Molecular Biology Reports 38, 4475-4482.
34. Yang H, Zhou Y, Zhang Y, Wang J, Shi H. 2019. Identification of transcription factors of nitrate reductase gene promoters and NRE2 cis-element through yeast one-hybrid screening in Nicotiana tabacum. BMC Plant Biology 19(1):145.
35. Zvi M.M, Shklarman E, Masci T, Kalev H, Debener T, Shafir S, Ovadis M, Vainstein A. 2012. PAP1 transcription factor enhances production of phenylpropanoid and terpenoid scent compounds in rose flowers. New Phytologist 195(2): 335-345.
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