Document Type : Research paper
Authors
1 Department of Horticultural Sciences, Islamic Azad University, Maragheh Branch, Maragheh, Iran
2 Department of Chemistry, Faculty of Science, University of Maragheh, Maragheh, Iran
Abstract
This study aimed to determine the amount of flavonoids, anthocyanins, total phenolic compounds, ascorbic acid and antioxidant activity of the peel and pulp of the quince (Cydonia oblonga Mill.) in the polar and nonpolar sub-fractions of methanol extracts with two extraction methods, Soxhlet and microwave. The antioxidant properties were assessed by the ability to quench the stable free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP) and β-carotene bleaching assays. The results showed that the Soxhlet method extracted higher amount of the phytochemicals. In the DPPH system, the highest radical scavenging activity was seen by the polar sub-fraction of the methanol extract in the peel of quince fruit (IC50=52±7.3 μg/mL). Among all the extracts analyzed, the polar sub-fraction of the peel extract exhibited a significantly higher total flavonoid (62.33±3.1 mg CTE/100 g), anthocyanin (1.54±0.24 μg C-3-GE/100 mg fresh weight), phenolic content (108.14 μg GAE/mg) and antioxidant activity than other samples with Soxhlet extraction method. Fruit has a number of bioactive ingredients and many of them were concentrated in the peel of the fruit rather than the flesh. Therefore, enjoy the benefits that unpeeled fruit offers! The present study revealed that the peel of quince fruit has higher phytochemicals and antioxidant properties when compared to the pulp.
Keywords
2. Alesiani D, Canini A, D’Abrosca B, Della-Greca M, Fiorentino A, Mastellone C, Monaco P, Pacifico S. 2010. Antioxidant and anti-proliferative activities of phytochemicals from quince (Cydonia vulgaris) peels. Food Chemistry 118, 199-207.
3. Amin F, Wani S.M, Gani A, Masoodi F.A. 2013. Polyphenolic estimation and antioxidant activity of some vegetables of J & K India-A Correlation Study. International Journal of Applied Engineering Research 3, 595-603.
4. Ashraf M.U, Muhammad G, Hussain M.A, Bukhari Syed-N.A. 2016. Cydonia oblonga M., A medicinal plant rich in phytonutrients for pharmaceuticals. Frontiers in Pharmacology 7, 1-20.
5. Benmeziane F, Djamai R, Cadot Y, Seridi R. 2014. Optimization of extraction parameters of phenolic compounds from Algerian fresh table grapes, (Vitis vinifera). International Food Research Journal 21(3), 1061-1065.
6. Carvalho M, Silva B.M, Silva R, Valentao P, Andrade P.B, Bastos M.L. 2010. First report on Cydonia oblonga Miller anticancer potential: differential anti-proliferative effect against human kidney and colon cancer cells. Journal of Agricultural and Food Chemistry 58, 3366-3370.
7. Charles D.J. 2013. Antioxidant properties of spices, herbs and other sources. Norway, IA, USA.
8. Cheng G.W, Breen P.J. 1991. Activity of phenylalanine ammonialyase (PAL) and concentrations of anthocyanins and phenolics in developing strawberry fruit. Journal of American Society Horticultural Science 116, 865-869.
9. Dourtoglou V.G, Mamalos A, Makris D.P. 2006. Storage of olives (Olea europaea) under CO2 atmosphere: Effect on anthocyanins, phenolics, sensory attributes and in vitro antioxidant properties. Food Chemistry 99, 342-349.
10. Jahanban-Sfahlan A, Mahmoodzadeh A, Hasanzadeh A. 2009. Antioxidants and antiradicals in almond hull and shell (Amygdalus
communis L.) as a function of genotype. Food Chemistry 115, 529-533.
11. Kim D.O, Chun O.K, Kim Y.J. 2003. Quantification of polyphenolics and their antioxidant capacity in fresh plums. Journal of Agricultural and Food Chemistry 51, 6509-6515.
12. Kulisic T, Radonic A, Katalinic V. 2004. Use of different methods for testing antioxidative activity of oregano essential oil. Food Chemistry 85, 633-640.
13. Magalhaes A.S, Silva B.M, Pereira J.A, Andrade P.B, Valentao P, Carvalho M. 2009. Protective effect of quince (Cydonia oblonga Miller.) fruit against oxidative hemolysis of human erythrocytes. Food Chemical Toxicology 47, 1372-1377.
14. Miller H.E. 1971. A simplified method for the evaluation of antioxidants. Journal of the American Oil Chemists' Society 48, 91-97.
15. Molyneux P. 2004. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology 26, 211-219.
16. Muzykiewicz A, Zielonka-Brzezicka J, Klimowicz A. 2018. Quince (Cydonia oblonga Mill.) as a useful source of antioxidants-antioxidant activity evaluation. Herba Polonica 64 (4), 23-33.
17. Pantelidis G.E, Vasilakakis M, Manganaris G.A. 2007. Antioxidant capacity, phenol, anthocyanin, and ascorbic acid contents in raspberries, blackberries, red currants, gooseberries and Cornelian cherries. Food Chemistry 102, 777-783.
18. Sharififar F, Moshafi M.H, Mansouri S.H. 2007. In vitro evaluation of antibacterial and antioxidant activities of the essential oil and methanol extract of endemic Zataria multiflora Boiss. Food Control 18, 800-805.
19. Silva B.M, Andrade P.B, Mendes G.C, Seabra R.M, Ferreira M.A. 2002. Phenolic profile of quince fruit (Cydonia oblonga Miller) (Pulp and Peel). Journal of Agricultural and Food Chemistry 50, 4615-4618.
20. Silva B.M, Andrade P.B, Valentao P, Ferreres F, Seabra R.M, Ferreira M.A. 2004. Quince (Cydonia oblonga). Miller) fruit (pulp, peel, and seed) and jam: antioxidant activity. Journal of Agricultural and Food Chemistry 52, 4705-4712.
21. Singleton V.L, Rossi J.A. 1965. Colorimetry of total phenolics with phosphomolybdic-
Free Radical Scavengering and Phenolic Compounds of Peel and Pulp of Quince 101
phosphotungstic acid reagents. American Journal of Enology and Viticulture 16, 144-158.
22. Sun J, Chu Y.F, Wu X. 2002. Antioxidant and antiproliferative activities of common fruits. Journal of Agricultural and Food Chemistry 50, 449-454.
23. Szydlowska-Czerniak A, Tulodziecka A. 2015. Optimization of ultrasound-assisted extraction procedure to determine antioxidant capacity of rapeseed cultivars. Food Analytical Methods 8, 778-789.
24. Toor R.K, Savage G.P. 2005. Antioxidant activity in different fractions of tomatoes. Food Research International 38, 487−494.
25. Wojdylo A, Oszmianski J, Bielicki P. 2013. Polyphenolic composition, antioxidant activity, and polyphenol oxidase (PPO) activity of quince
(Cydonia oblonga Miller) varieties. Journal of Agricultural and Food Chemistry 61 (11), 2762-2772.
26. Wu Y, Wang X, Xue J, Fan E. 2017. Plant phenolics extraction from flos Chrysanthemi: response surface methodology based optimization and the correlation between extracts and free radical scavenging activity. Journal of Food Science 82(11), 2726-2733.
27. Gu L. 2012. Analysis methods of proanthocyanidins. In: Xu Z, Howard LR (eds), Analysis of antioxidant-rich phytochemicals. Wiley-Blackwell. Chichester.
28. Yildirim A, Mavi A, Kara A. 2001. Determination of antioxidant and antimicrobial activities of Rumex crispus L. extracts. Journal of Agricultural and Food Chemistry 49, 4083-4089.
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