Effects of Scion Cultivar, Rootstock Age and Hormonal Treatment on Minigrafting of Persian Walnut

Document Type: Research paper


1 Department of Horticultural Sciences, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.

2 Temperate Fruits Research Center, Horticultural Science Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran


The experiment was carried out during 2015-2016, with the aim of investigating the effects of scion cultivar, rootstock age and hormonal treatments on the success of walnut minigrafting. Analysis of results showed that all studied factors were effective on graft-take and survival of minigrafted walnuts and other growth traits of the scions. The highest graft-take was observed on one-year-old rootstocks in hormonal treatments of 20 mg/L BA + 12.5 mg/L IBA for ‘Jamal’ and 80 mg/L BA + 50 mg/L IBA for ‘Chandler’. The highest survival rate was observed on one-year-old rootstocks in control treatment and the lowest was on two-year-old rootstocks in hormonal treatment of 80 mg/L BA + 50 mg/L IBA. The highest growth rate of scion belonged to the scions of ‘Jamal’ on one-year-old rootstocks in hormonal treatment of 20 mg/L BA + 12.5 mg/L IBA and the lowest was in scions of ‘Chandler’ on two-year-old rootstocks in hormonal treatments of 20 mg/L BA + 12.5 mg/L IBA and 80 mg/L BA + 50 mg/L IBA. In addition, diameter of the graft union on two-year-old rootstocks was more than on one-year-old ones. Histological studies showed that increase in the diameter of the graft unit on two-year-old rootstocks was due to dead and necrotic tissues at the upper parts of it which acted as a barrier against passage of water and minerals from the rootstock to the scion and reduced the growth of the scion on two-year-old rootstocks.


Aloni B, Karni L, Deventurero G, Levin Z, Cohen R, Katzir N, Lotan-Pompan M, Edelstein M, Aktas H, Turhan E, Joel D.M, Horev C, Kapulnik Y. 2008. Physiological and biochemical changes at the rootstock-scion interface in graft combinations between Cucurbita rootstocks and a melon scion. The Journal of Horticultural Science and Biotechnology 83, 777-783.

Aloni R. 1987. Differentiation of vascular tissues. Annual Reviews Plant Physiology 38, 179-204.

Aloni R. 1995. The induction of vascular tissues by auxin and cytokinin. In: Davies P.J. (Ed.) Plant Hormones: Physiology, Biochemistry and Molecular Biology. Kluwer Academic Publishers, Dordrecht, The Netherlands. pp 531-546.

Aloni R. 2001. Foliar and axial aspects of vascular differentiation hypothesis and evidence. Journal of Plant Growth Regulation 20, 22-24.

Aloni R. 2010. The induction of vascular tissues by auxin plant hormones. In: Davies P.J. (Ed.) Plant Hormones: Biosynthesis, Signal Transduction and Action. Kluwer Academic Publishers, Dordrecht, The Netherlands. pp 485-518.

Alzate A, Royero N, Nunez V, Cabral J, Tohme1 J, Mejia-Jimenez A. 2002. Optimization of the in vitro propagation methodology of selected clones of soursop (Annona muricata L.) and evaluation of the compatibility of different scion and rootstock combinations for in vitro micrografting. Centro Internacional de Agricultura Tropical- CIAT, Cali, CO.

Aminzadeh F. 2012. An Investigation on Minigrafting of Walnut in Semi-Controlled Conditions. University of Tehran, Iran. M.Sc. Thesis. P 113.

Andrews P.K, Marquez C.S. 1993. Graft incompatibility. Horticultural Reviews 15, 183-232.

Arteca R.N. 1996. Plant Growth Substances: Principles and Applications. Chapman & Hall, New York. P 332.

Avanzato D, Atefi J. 1997. Walnut grafting by heating the graft point directly in the field. Acta Horticulturae 442, 291-294.

Balanian H. 2010. Walnut Minigrafting in Controlled Conditions. University of Tehran, Iran. M.Sc. Thesis. P 110.

Carlson V. 1963. How to green graft grapes. Univ. of California, USA. P 115.

Dehghan B, Vahdati K, Hassani D, Rezaee R. 2010. Bench-grafting of Persian walnut as affected by pre- and post-grafting heating and chilling treatments. The Journal of Horticultural Science and Biotechnology 85(1), 48-52.

Dolgun O, Tekintas F.E, Ertan E. 2008. A histological investigation of graft union in some plum varieties grafted on pixy rootstock. ADu Ziraat Fakultesi Dergisi 5, 1-4.

Ebrahimi A, Vahdati K, Fallahi E. 2006. Improved success of Persian walnut grafting under environmentally controlled conditions. International Journal of Fruit Science 6, 3-12.

Errea P. 1998. Implications of phenolic compounds in graft incompatibility in fruit tree species. Scientia Horticulturae 74, 195-205.

FAO. 2014. FAOSTAT database results. http://faostat3.Fao.org.faostat.

Foyer C.H, Noctor G. 2003. Redox sensing and signaling associated in plants: A reevaluation of the concept of oxidative stress response in leaves. Journal of Experimental Botany 53, 1249-1254.

Gandev S. 2007. Budding and grafting of the walnut (Juglans regia L.) and their effectiveness in Bulgaria (Review). Bulgarian Journal of Agricultural Science 13, 683-689.

Gandev S, Arnaudov V. 2011. Propagation method of epicotyl grafting in walnut (Juglans regia L.) under production condition. Bulgarian Journal of Agricultural Science 17, 173-176.

Ghamari Hesabi F, Sharafi Y, Tabatabaei S.J, Grigurian V. 2016. Effect of budding method, rootstock age and cut below budding union on budding success in Persian walnut. Journal of Nuts 7(2), 119-124.

Gross G.G, Janse C, Elstner E.F. 1977. Involvement of malate, monophenols, and the superoxide radical in hydrogen peroxide formation by isolated cell walls from horseradish (Amoracia lapathifolia Gilib.). Planta 136, 271-276

Hartmann H.T, Kester D.E, Davies F.T.J.R, Geneve L.R. 2002. Plant Propagation: Principles and Practices. Seventh Edition. Regents / Prentice Hall International Editions, Englewood Cliffs, New Jersey. P 880.

Karadeniz T. 2005. Relationships between graft success and climatic values in walnut (Juglans regia). Journal of Central European Agriculture 6, 631-634.

Kazankaya A, Mehmet S, Tekintas F.G. 1997. Relations between graft success and structural hormones on walnut (J. regia L.). Acta Horticulturae 442, 295-298.

Köse C, Güleryüz M. 2006. Effects of auxins and cytokinins on graft union of grapevine (Vitis vinifera). New Zealand Journal of Crop and Horticultural Science 34(2), 145-150.

Kuniyuki A, Forde H. 1985. Walnut propagation. In: Ramos D. (Ed.). Walnut Orchard Management. Publication 21410, University of California, USA. pp 38-46.

Lu S.F, Yang S.J. 1995. Electrical wave transmission between stock and scion. Acta Phytophisiologica Sinica 21, 386-392.

Lu S.F, Tang D.T, Song J.Y, Liu M.Q, Yang S.J. 1996. Preliminary studies on controlling graft union through plant hormones. Acta Botanica Sinica 38, 307-311.

Macheix J.J, Fleuriet A, Quessada M.P. 1986. Involvement of phenols and peroxidases in wound healing and grafting. In: Greppin H, Penel C, Gaspar T.H (Eds). Molecular and Physiological Aspects of Plant Peroxidases. Univ. of Geneva, Switzerland. P 267.

Manusev B. 1970. Studies on some method of grafting walnuts in the region of Force. Horticulture Abstract 41, 6040.

Mattsson J, Ckurshumova W, Berlet T. 2003. Auxin signaling in Arabidopsis leaf vascular development. Plant Physiology 131, 1327-1339.

Mehmet S, Kazankaya A, Testereci H, Hakkiyoruk I. 1997. Changing of IAA (Indol-3-Acetic Acid) content at different organs of walnut (Juglans regia L.), after grafting. Acta Horticulturae 422, 169-174.

Mittler R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends in Plant Sciences 7, 405-410.

Mng'omba S.A, Du Toit E.S, Akinnifesi F.K, Venter H.M. 2007. Histological evaluation of early graft compatibility in Uapaca kirkiana Müell Arg. scion/stock combinations. HortScience 42(3), 1-5.

Moore R. 1984. A model for compatibility-incompatibility in higher plants. American Journal of Botany 71(5), 752-758.

Mulkey T.J, Kuzmanoff K.M, Evan M.L. 1982. Promotion of growth and shift in the auxin dose/response relationship in maize roots treated with the ethylene biosynthesis inhibitors aminoethoxyvinylglycine and cobalt. Plant Science Letters 25, 43-48.

Ozkan Y, Edizer Y, Akca Y. 2001. A study on propagation with patch budding of some walnut cultivars (Juglans regia L.). Acta Horticulturae 544, 521-525.

Pinghai D, Rongting X. 1993. Effect of phenols on survival of walnut grafting. Acta Horticulturae 311, 134-140.

Pirkhezri M, Hasani D, Soleimani A. 2010. Effects of IBA and GA3 on increasing the efficiency of patch budding in some Persian walnut cultivars. Horticultural Science 24(1), 64-69. (In Persian)

Prataviera A.G, Kuniyank A.H, Ryogo K. 1983. Growth inhibitors in xylem exudates of Persian walnut and their possible role in graft failure. Journal of the American Society for Horticultural Science 108, 1043-1045.

Rahman A, Amakawa T, Goto N, Tsurumi S. 2001. Auxin is a positive regulator of ethylene-mediated response in the growth of Arabidopsis roots. Plant and Cell Physiology 42, 301-307.

Rezaee R, Vahdati K. 2008. Introducing a simple and efficient procedure for top working Persian walnut trees. Journal of the American Pomological Society 62, 21-26.

Rezaee R, Vahdati K, Grigoorian V, Lizade M. 2008. Walnut grafting success and bleeding rate as affected by different grafting methods and seedling vigor. The Journal of Horticultural Science and Biotechnology 83, 94-99.

Rongting X, Pinghai D. 1990. Theory and practice of walnut grafting. Acta Horticulturae 284, 69-89.

Rongting X, Pinghai D. 1993. A study on the uniting process of walnut grafting and the factors affecting. Acta Horticulturae 311, 160-170.

Rongting X. 1993. A study on the uniting process of walnut grafting and the factors affecting. Acta Horticulturae 311, 160-172.

Sachs T. 1981. The control of the patterned differentiation of vascular tissue. Advances in Botanical Research 9, 151-262.

Solar A, Stampar F, Trost M, Barbo J, Avsec S. 2001. Comparison of different propagation methods in walnut (Juglans regia L.) made in Slovenia. Acta Horticulturae 544, 527-530.

Soleimani A, Rabiei V, Hassani D. 2010. Effect of different techniques on walnut (J. regia L.) grafting. Journal of Food, Agriculture and Environment 8(2), 544-546.

Soleimani A, Rabiei V, Hassani D, Amiri M.E. 2009. Effects of rootstock and cultivar on propagation of Persian walnut (Juglans regia L.) using hypocotyle grafting. Seed & Plant Production 25(1), 93-101. (In Persian)

Sorce C, Massai R, Picciarelli P, Lorenzi R. 2002. Hormonal relationships in xylem sap of grafted and ungrafted Prunus rootstocks. Scientia Horticulturae 93, 333-342.

Stanisavljevic M, Mitrovic M. 1995. Effect of variety on success grafting and development of nursery trees of walnut (J. regia). Acta Horticulturae 442, 281-283.

Taylor R.M. 1972. Influence of gibberellic acid on early patch budding of pecan seedling. Journal of the American Society for Horticultural Science 97(5), 677-679.

Tshokoeva M.D, Tsonev R.V. 1995. Compatibility of rootstock and scions in apricots trees. Acta Horticulturae 384, 471-476.

Vahdati K. 2003. Nursery management and grafting of walnut. Khaniran Publication, Tehran, Iran. P 99. (In Persian)

Vahdati K. 2006. Evaluation of side-stub and hypocotyl grafting efficiency for walnut propagation in Iran. Acta Horticulturae 705, 347-351.

Zakinthinos G, Rouskas D. 1997. Specific treatment on walnut grafting improvement. Acta Horticulturae 442, 285-289.

Zheng B.S, Chu H.L, Jin S.H, Huang Y.J, Wang Z.J, Chen M, Huang J.Q. 2010. cDNA-AFLP analysis of gene expression in hickory (Carya cathayensis) during graft process. Tree Physiology 30, 297-303.