Document Type : Research paper

Authors

1 Ornamental Plants Research Center (OPRC), Horticulture Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Mahallat, Iran

2 Department of Plant Production and Genetic (Biotechnology), College of Agriculture, Jahrom University, Jahrom, Iran

Abstract

To produce new, high-quality offsprings, we generated two cross combinations in Iris germanica. Three parental plants were selected after preliminary screening of commercial traits in different cultivars. Fourteen quantitative traits mostly related to the flower organ were investigated on 15 progenies and three parental plants in a randomized complete block design with three replications. Significant positive and negative correlations were observed among different studied attributes at 0.01 and 0.05 probability. The highest positive (+0.86) and negative (-0.76) correlations was recorded between diameter of flowering branch with crown diameter as well as peduncle length, respectively. Cluster analysis using Ward’s method separated most of the progenies from the parental plants based on evaluated morphological characteristics. However, some of the progenies were grouped with their maternal parent. In PCA analysis, five factors explained 87.84% of the total variations; crown diameters as well as diameter of flowering branch were predominant in the first component (34.19%). Some promising progenies with interesting characteristics were observed and several hybrid plants were superior to their parents and showed positive heterosis over their parents. Progenies NIOP5 and NIOP8 were superior in the most studied attributes, therefore, they suggested for further investigations which can be used in breeding programs. Progenies showed a wider range for flower diameter, peduncle thickness, bush height, as well as inner and outer tepal dimensions. Flower color also showed wider range in the progenies and purple, violet and white colors were the most frequent colors in the hybrid plants, while lilac color was the rarest one. Results of our investigation revealed that intervarietal hybridization is an effective way to contribute to the phenotypic variation in the iris flower for producing new plant materials for breeding purposes as well as releasing new cultivars. The inheritance of some of the traits identified in this study will be important for improving plant architecture in German iris plant.

Keywords

Arnold M.I, Tang S, Knapp S.J, Martin N.H. 2010. Asymmetric introgressive hybridization among louisiana Iris species. Genes 1(1), 9-22. DOI: 10.3390/genes1010009.
Arzani K. 1994. Horticultural and Physiological Aspects of Vigor Control in Apricot (Prunus armeniaca L.) under Orchard and Controlled Environment Conditions. PhD dissertation, Department of Plant science research at Massey University, Palmerston North, New Zealand.
Austin C. 2005. Irises a Gardener’s Encyclopedia. Portland. U.S.A. pp 340.
Azimi M.H, Moradiashur B, Hosseini-Nia A. 2011. Genetic variation and heritability of some traits in Wild Iranian Iris species. Iranian, Journal of Rangelands and Forests Plant Breeding and Genetic Resorces 19(1), 153-166. (In Persian)
Azimi M.H, Sadeghian S.Y, Razavi Ahari V, Khazaei F, Fathi Hafashjani A. 2012. Genetic variation of Iranian Iris species using morphological characteristics and RAPD markers. International Journal of Agricultural Science 9(2), 875-889.
Azimi M.H, Sadeghyan S.Y, Beyramizadeh E, Kalate-Jari S, Tahernezhad Z. 2010b. Study of genetic variation among Iranian Irises species using morphological characteristics. Iranian Journal of Horticultural Science & Technology 11(1), 71-86. (In Persian)
Bell R.L, Quamme H.A, Layne R.E.C, Skirvin R.M. 1996. Pears. In: "Tree and Tropical Fruits, Fruit Breeding", (Eds.): Janick J, Moore J.N, John Wiley and Sons, Inc., NY, USA, PP. 441-514.
Burke J.M, Carney S.E, Arnold M.L. 1998. Hybrid in the loisiana Irises. Evolution 52(1), 37-43.
Ghanadi F. 1991. Iris flower training. Flowers Press. p 115. (In Persian)
Gozu Y.M, Yokoyama M, Nakamura R, Namba K, Yomogida M, Nakamura Y. 1993. In vitro propagation of Iris pallid. Plant Cell Reports 13, 12-16.
Ha M, Lu J, Tian L, Ramachandran V, Kasschau K.D, Chapman E.J, Carrington J.C, Chen X, Wang X.J, Chen Z.J. 2009. Small RNAs serve as a genetic buffer against genomic shock in Arabidopsis interspecific hybrids and allopolyploids. Proceedings of the National Academy of Sciences, USA 106, 17835-17840.
Hallauer A.R, Carena M, Miranda J.B. 1988. Quantitative genetics in maize breeding. Hand book of plant breeding, Iowa State University Press, IOWA, USA. pp 1-31.
Hayakawa H, Muroi M, Hamachi H, Yokoyama J, Fukuda T. 2011. Correlation of variation between leaf and flower characters in Cymbidium goeringii (Rchb. f.) Rchb. f. (Orchidaceae). Journal of Japanes Botany 86, 82-92.
Iezzoni A.F, Pritts M.P. 1991. Applications of principal components analysis to horticultural research. Horticulture Science 26, 334-338.
International Union for the Protection of New Varieties of Plants (UPOV). 2000. Guidelines for the conduct of test for distinctness, uniformity and stability, iris L., TG/174/3, 2000-04-05. Geneva.
Ishikura N. 1980. Anthocyanins in iris flowers. Cellular and Molecular Life Sciences CMLS 36(6):656-657. DOI: 10.1007/BF01970120
Jalilian H, Zarei A, Erfani-Moghadam J. 2018. Phylogeny relationship among commercial and wild pear species based on morphological characteristics and SCoT molecular markers. Scientia Horticulturae. 235: 323-333. https://doi.org/10.1016/j.scienta.2018.03.020.
Joshi S.P, Bhave S.G, Chowdari K.V, Apte G.S, Dhonukshe B.L, Latitha K, Ranjekar P.K, Gupta V.S. 2001. Use of DNA markers in prediction of hybrid performance and heterosis for a three-line hybride system in rice. Biochemical Genetic 39, 179-199.
Jozghasemi S, Rabiei V, Soleymani A. 2015. Evaluation of the pigments concentration in the Iris species native to Iran. Journal of Biodiversity and Environmental Science 6(1), 557-561.
Kasperbauer M.J. 1990. Biotechnology in tall fescue improvement. CRC Press. Inc. Florida. pp 199.
Kirk H, Vrieling K, Klinkhamer P.G.L. 2005. Maternal effects and heterosis influence the fitness of plant hybrids. New Phytologist 166, 685-694
Kumar Baranwal V, Mikkilineni V, Barwale Zehr U, Tyagi A.K, Kapoor S. 2012. Heterosis: emerging ideas about hybrid vigour. Journal of Experimental Botany 63(18), 6309-6314. DOI:10.1093/jxb/ers291.
Luo S, He P. Zheng X, Zhou P. 2002. Inheritance of RAPD markers in an interspecific F1 hybrid of grape between Vitis quinquangularis and V. vinifera. Scientia Horticulturae 93, 19-28.
Mizunoa T, Uehara A, Mizuta D, Yabuya T, Iwashina T. 2015. Contribution of anthocyanin–flavone copigmentation to grayed violet flower color of Dutch iris cultivar ‘Tiger's Eye’ under the presence of carotenoids. Scientia Horticulturae, 186, 201-206. DOI: https://doi.org/10.1016/j.scienta.2015.01.037.
Musavi Bazaz A, Nemati H, Tehranifar A, Hatefi S. 2007. Study of hybridization and correlation among morphological and ornamental characters in Mathiola genotype. 5th Horticulture congress, Shiraz, Iran. (In Persian).
Norman P.E, Tongoona P, Shanahan P.E. 2011. Determination of interrelationships among agr-morphological traits of yams (Discorea spp.) using correrlation and factor analyses. Journal of Applied Bioscience 45, 3059-3070
Norusis M.J. 1998. SPSS/PC advanced statistics. SPSS Inc, Chicago.
Rahimi V, Arab M, Deanati SH, Amiri R. 2009. Study of genetic variation among Iranian Iris species using morphological characteristics. 6th Horticulture congress, Rasht, Iran. (In Persian)
SAS® Procedures. 1990. Version 6, 3rd edn. SAS Institute, Cary
Shimizu K, Miyabe Y, Nagaike H, Yabuya T, Adachi T. 1999. Production of somatic hybrid plants between Iris ensata and Iris germanica.Euphytica 107, 105-113
Strecker J, Rodríguez G, Brewer M, Gonzalo M.J, Anderson C, Lang L, Sullivan D, Wagner E, Strecker B, Drushal R, Dujmovic N, Fujimuro K, Josh Thomas J, Gray S, Knaap E. 2010. Tomato Analyzer User Manual Version 3. Ohaio University.
Tanaka Y, Katsumoto Y, Brugliera F, Mason J. 2005. Genetic engineering in floriculture. Plant Cell, Tissue and Organ Culture 80, 1-24
Wendelbo P. 1977. Tulips and Irises of Iran and Their Relatives. Botanical Institute of Iran. pp 83.
Werlemark G, Uggla M, Nybom H. 1999. Morphological and RAPD markers show a highly skewed distribiution in a pair of reciprocal crosses between hemisexual dogrose species, Rosa sect. Caninae. Theoretical and Applied Genetics 98, 557-563.
Yang Y, Wen C, Ma N, Zhao L. 2015. Heterosis and genetic analysis of branching in cut-flower chrysanthemums. Euphytica DOI: 10.1007/s10681-015-1439-7.
Yuval S, Avi S, Orif H, Prter C. 2002. Morphological variation of the Oncocyclus Irises (Iris: Iridaceae) in the southern Levant. Botanical Journal of the Linnean Society 139(4), 369-382.
Zamani Z, Zarei A, Fatahi R. 2010. Characterization of progenies derived from pollination of pomegranate cv. Malase-Tourshe-Saveh using fruit traits and RAPD molecular marker. ScientiaHorticulturae, 124, 67-73. ISI DOI: 10.1016/j.scienta.2009.12.021.
Zarei A. 2017. Biochemical and pomological characterization of pomegranate accessions in Fars province of Iran. SABRAO Journal of Breeding and Genetics, 49(2), 155-167.
Zhen H.S, Yin G, Lin H.Y. 1997. The hybridization of Iris spp. Journal of Plant Resources andEnvironment 1998:01.
Zhen H.S, Yin G, Lin H.Y. 2003. Breeding dwarf plants of Iris germanica L. through hybridization. Journal of Plant Resources and Environment 04.