Document Type: Research paper


Pistachio Research Center, Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rafsanjan, Iran.


Understanding mechanisms of salt tolerance, physiological responses to salt stress, and screening genotypes for breeding programs are important scientific issues remained to be investigated in pistachio. Therefore, current study was carried out to investigate response of different pistachio cultivars (G1, G2, Kaleghochi and UCB1) to salinity treatments (0.6 as control, 10, 20 dS m-1 using saline underground water) as a factorial experiment based on randomized complete block design with three replications in greenhouse of Iranian Pistachio Research Institute (Rafsanjan) in 2014-2015. Some physiological and nutrition properties of the pistachio cultivars measured in this study. Results showed decreased stomatal conductance, photosynthesis rate, chlorophyll content, and Fv/Fm in response to salinity treatments. The main cause of these changes was related to the altered ion contents along with the competition among ions for being absorbed by plant. Despite of sufficient amount of potassium in the soil, high concentrations of sodium and other associated elements such as calcium and magnesium decreased the ability of pistachio plants to absorb adequate amount of vital ions such as potassium. As a result of sodium accumulation and deficiency of potassium, K+/Na+ ratio was decreased in pistachio leaves. Consequently, toxicity of sodium ions in the plant cells reduced stomatal conductance and the rate of photosynthesis. Comparison between cultivars showed that for the most of the traits the difference between control and moderate salinity (10 ds m-1) in all cultivars was not significant. However, G2 cultivar showed higher ability to accumulate potassium and absorbed lower concentration of sodium, calcium, and magnesium under sever salinity treatment (20 ds m-1). These result suggested that G2 could be considered as a potential tolerant cultivar for cultivation in saline area.


Adish M, Fekri M, Hokmabadi H. 2010. Response of Badami-Zarand pistachio rootstock to salinity stress International Journal of Nuts Related Sciences 1, 1-11.

Alipur H, Hosseini J, Ghaffari F. 2012. Determining salinity tolerance in female pistachio cultivars using controlled crosses. Journal of Plant Production Reserach 19, 39-59.

Attarzadeh M, Rahimi A, Torabi B. 2016. Response of chlorophyll, relative water content and protein percentage of safflower leaves to salinity and foliar calcium, potassium and magnesium applications. Research and Science Journal of Crop Ecophysiology 9, 269-288.

Bastam N, Baninasab B, Ghobadi C. 2013. Interactive effects of ascorbic acid and salinity stress on the growth and photosynthetic capacity of pistachio seedlings. Journal of Horticultural Science and Bioetecnology 88, 610-616.

Bernstein L. 1975. Effects of salinity and sodicity on plant growth. Annual Review of Phytopathol 13, 295-312.

Deinlein U, Stephan A.B, Horie T, Luo W, Xu G, Schroeder J. 2014. Plant salt-tolerance mechanisms. Trends in Plant Science 19, 371-379.

Falah A, Farahmandfar A, Moradi F. 2015. Effect of salinity stress in different growth stages on some morpho-physiological properties of two cultivars of rice under greenhouse conditions. Agriculture 107, 175-184.

Galvan-Ampudia C.S, Julkowska M.M, Darwish E, Gandullo J, Korver R.A, Brunoud G, Haring M.A, Munnik T, Vernoux T, Testerink C. 2013. Halotropism is a response of plant roots to avoid a saline environment. Current Biology 23, 2044-2050.

Garcıa-Sánchez F, Carvajal M, Porras I, Botıa P, Martınez V. 2003. Effects of salinity and rate of irrigation on yield, fruit quality and mineral composition of ‘Fino 49’ lemon. European Journal of Agronomy 19, 427-437.

Hokmabadi H, Sharafati A. 2015. The effect of some pistachio based on absorption of nutrients in two pistachio cultivars including Akbari and Barghsiah. Pistachio Journal of Science and Technology 1, 32-43.

Hossain M.A, Bhattacharjee S, Armin S.M, Qian P, Xin W, Li H.Y, Burritt D.J, Fujita M, Tran L.S.P. 2015. Hydrogen peroxide priming modulates abiotic oxidative stress tolerance: insights from ROS detoxification and scavenging. Frontiers in Plant Science 6, 420-438.

Jalili Marandi R, Hasani A, Dulati Baneh H, Haji Taghiloo R, Yusef-Zadeh H. 2012. The effect of salinity on morphological and physiological properties of the two grape cultivars. Journal of Horticultural Science 6, 68-77.

Karimi S, Rahemi M, Maftoun M, Tavallali V. 2009. Effects of long-term salinity on growth and performance of two pistachio (Pistacia vera L.) rootstocks. Australian Journal of Basic and Applied Sciences 3, 1630-1639.

Khan H.A, Pervez M.A, Ayub C.M, Ziaf K, Balal R.M, Shahid M.A, Akhtar N. 2009. Hormonal priming alleviates salt stress in hot pepper (Capsicum annuum L.). Soil and Environment 28, 130-135.

Laohavisit A, Richards S.L, Shabala L, Chen C, Colaço R, Swarbreck S.M, Shaw E, Dark A, Shabala S, Shang Z. 2013. Salinity-induced calcium signaling and root adaptation in Arabidopsis require the calcium regulatory protein annexin1. Plant Physiology 163, 253-262.

Mozaffari V, Eskandari S, Tajabadi A, Dashti H. 2015. the effect of salinity levels and copper on some nutrients uptake in shoot and root of two cultivars of pistachio (Pistacia vera L.). Pistachio Science and Technology Journal 1, 14-31.

Najafian S.H, Rahemi M, Tavallali V. 2008. Effect of salinity on tolerance of two bitter almond rootstock. Journal of Environmental Sciense and Agriculture 3, 264-268.

Oukarroum A, El Madidi S, Schansker G, Strasser R.J. 2007. Probing the responses of barley cultivars (Hordeum vulgare L.) by chlorophyll a fluorescence OLKJIP under drought stress and re-watering. Environmental and Experimental Botany 60, 438-446.

Paknejad F, Nasri M, Moghadam H.R.T, Zahedi H, Alahmadi M.J. 2007. Effects of drought stress on chlorophyll fluorescence parameters, chlorophyll content and grain yield of wheat cultivars. journal of Biological Science 7, 841-847.

Pirasteh‐Anosheh H, Saed‐Moucheshi A, Pakniyat H, Pessarakli M. 2016. Stomatal responses to drought stress, Water stress and crop plants: a sustainable approach. Whiley, New York, USA, pp. 24-40.

Ranjbar A, Lemeur R, Van Damme P. 2001. Ecophysiological characteristics of two pistachio species (Pistacia khinjuk and Pistacia mutica) in response to salinity. Acta Horticulturae 72, 343-349.

Rezaei M, Lesani H, Babalar M, Talaei A. 2006. Effects of sodium chloride stress on growth and the concentration of five olive cultivars. Iranian Journal of Agricultural Sciences 37, 293-301.

Saed-Moocheshi A, Shekoofa A, Sadeghi H, Pessarakli M. 2014. Drought and salt stress mitigation by seed priming with Kno3 and urea in various maize hybrids: an experimental approach based on enhancing antioxidant responses. Journal of plant nutrition 37, 674-689.

Sajedi Nia A. 2008. studying the Ecophysiology properties of Six Iranian pistachio cultivars (Pistacia vera L.), Department of Horticulture. College of Agriculture, Bu-Ali Sina University, Hamedan, Iran, p. 78.

Sevengor S, Yasar F, Kusvuran S, Ellialtioglu S. 2011. The effect of salt stress on growth, chlorophyll content, lipid peroxidation and antioxidative enzymes of pumpkin seedling. African Journal of Agricultural Research 6, 4920-4924.

Tavallali V, Rahemi M, Maftoun M, Panahi B, Karimi S, Ramezanian A, Vaezpour M. 2009. Zinc influence and salt stress on photosynthesis, water relations, and carbonic anhydrase activity in pistachio. Scientia Horticulturae 123, 272-279.

Tavallali V, Rahemi M, Panahi B. 2008. Calcium induces salinity tolerance in pistachio rootstocks. Fruits 63, 285-296.

Yuan S, Lin H.H. 2008. Minireview: role of salicylic acid in plant abiotic stress. Journal of plant physiology 63, 313-320.

Zadsalehimasouleh F, Mozafari V, Tajabadipour A, Hokmabadi H. 2014. Pistachio responses to salt stress at varied levels of magnesium. Journal of Plant Nutrition 37, 889-906.