Resistance and vegetative growth analysis of some olive cultivars in response to a defoliating pathotype of Verticillium dahliae Kleb

Document Type: Research paper

Authors

Department of Plant Protection, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

Abstract

Verticillium dahliae Kleb. the causal agent of vascular wilt is an important pathogen of olive trees in growing areas of the world. Nine-month-old nursery olives including ‘Bladi’, ‘Conservalia’, ‘Kalamon’, ‘Koroneiki’, ‘Manzanilla’, ‘Mission’, ‘Marry’, ‘Picual’, ‘Rowghani’, ‘Sevillana’ and ‘Zard’ cultivars were root-dip inoculated with a defoliating (VCG1) isolate of V. dahliae obtained from diseased olives. Resistance was evaluated by assessing symptom severity using 0-4 rating scale and estimating the area under disease progress curves. The percentage of plants killed and final mean severity of symptoms were used as additional parameters for classifying the cultivars. Most of the tested cultivars were susceptible or highly susceptible to Verticillium wilt. However, two genotypes ‘Kalamon’ and ‘Koroneiki’ exhibited high resistance or resistance to this disease. Vegetative growth was reduced in inoculated plants due to infections caused by V. dahliae, although the reduction was remarkably higher in susceptible than in resistantcultivars. Correlation coefficient analyses revealed a negative relation between disease severity and vegetative growth. Dendrogram of olive cultivars based on all parameters represented two main clusters, major and minor. Minor cluster comprised only ‘Kalamon’ and ‘Koroneiki’ cultivars as resistant to V. dahliae. The major cluster could be divided into two groups, exhibiting extremely susceptible or susceptible reaction to V. dahliae.

Keywords


Anonymous. 2015. Agriculture Information for 2015, vol. 2. Jahad and Agricultural Ministry, Tehran.

Antoniou P.P, Markakis E.A, Tjamos S.E, Paplomatas E.J, Tjamos E.C. 2008. Novel methodologies in screening and selecting olive varieties and root-stocks for resistance to Verticillium dahliae. European Journal of Plant Pathology122, 549-560.

Barranco D, Trujillo I, Rallo P. 2000. Are ‘Oblonga’ and ‘Frantoio’ olives the same cultivar? Horst Science 35, 1323-1325.

Bejarano-Alcázar J, Blanco-López M.A, Melero-Vara J.M, Jiménez-Díaz R.M. 1996. Etiology, importance, and distribution of verticillium wilt of cotton in southern Spain. Plant Disease80, 1233-1238.

Birem F, Alcántara-Vara E, LÓpez-Escudero F.J. 2016. Water consumption and vegetative growth progress in resistant and susceptible olive cultivars infected by Verticillium dahliae. Agricultural Sciences 7, 230-238.

Blanco-LÓpez M.A, Jiménez-Díaz R.M, Caballero J.M. 1984. Symptomatology, incidence and distribution of Verticillium wilt of olive trees in Andalucıa. Phytopathologia Mediterranea 23, 1-8.

Bubici G, Cirulli M. 2012. Control of Verticillium wilt of olive by resistant rootstocks. Plant Soil 352, 363-376.

Caballero J.M, Del Rio C. 2008. The olive world germplasm bank of Spain. Acta Horticulture 791, 31-38.

CalderÓn R, Lucena C, Trapero-Casas J.L, Zarco-Tejada P.J, Navas-Cortés J.A. 2014. Soil temperature determines the reaction of olive cultivars to Verticillium dahliae pathotypes. PLoS ONE 9(10), e110664.

Campbell C.L, Madden L.V. 1990. Introduction to Plant Disease Epidemiology. John Wiley, New York.

Cirulli M, Montemurro G. 1976. A comparison of pathogenic isolates of Verticillium dahliae and sources of resistance in olive. Agriculturae Conspectus Scientificus 39, 469-476.

Colella C, Miacola C, Amenduni M, D’Amico M, Bubici G, Cirulli M. 2008. Sources of verticillium wilt resistance in wild olive germplasm from the Mediterranean region Plant Pathology57, 533-539.

Erten L, Yıldız M. 2011. Screening for resistance of Turkish olive cultivars and clonal rootstocks to Verticillium wilt. Phytoparasitica 39, 83-92.

Garcí-Ruiz G.M, Trapero C, Del Río C, LÓpez-Escudero F.J. 2014. Evaluation of resistance of Spanish olive cultivars to Verticillium dahliae in inoculations conducted in greenhouse. Phytoparasitica 42, 205-212.

Garcí-Ruiz G.M, Trapero C, Varo-Surtez Á, Trapero A, LÓpez-Escudero F.J. 2015. Identifying resistance to Verticillium wilt in local Spanish olive cultivars. Phytopathologia Mediterranea 54, 453-460.

Goicoechea N. 2006. Verticillium-Induced wilt in pepper: physiological disorders and perspectives for controlling the disease. Plant Pathology Journal 2, 258-265.

Hiemstra J, Harris D. 1998. Compendium of Verticillium Wilt in Tree Species, Ponsen & Looijen: Wageningen, the Netherlands.

Jiménez-Díaz R.M, Cirulli M, Bubici G, Jiménez-Gasco M.D, Antoniou P.P, Tjamos E.C. 2012. Verticillium wilt, a major threat to olive production: current status and future prospects for its management. Plant Disease 96, 304-329.

Karagiannidis N, Bletsos F, Stavro Poulos N. 2002. Effect of Verticillium wilt and mycorrhiza (Glomus mosseae) on root colonization, growth and nutrient uptake in tomato and eggplant seedlings. Scientia Horticulturae 94, 145-156.

Levin A.G, Lavee S, Tsror L. 2007. The influence of salinity on Verticillium dahliae in stem cuttings of five olive cultivars. Journal of Phytopathology 155, 587-592.

Levin A.G, Lavee S, Tsror L. 2003. Epidemiology of Verticillium dahliae on olive (cv. Picual) and its effect on yield under saline conditions. Plant Pathology 52, 212-218.

LÓpez-Escudero F.J, Blánco-LÓpez M.A. 2001. Effect of a single or double soil solarization to control verticillium wilt in established olive orchards in Spain. Plant Disease 85, 489-496.

LÓpez-Escudero F.J, del Rio RincÓn C, Caballero Reig J.M, Blanco-LÓpez M.A. 2004. Evaluation of olive cultivars for resistance to Verticillium dahliae. European Journal of Plant Pathology 110, 79-85.

LÓpez-Escudero F.J, Blánco-LÓpez M.A. 2007. Relationship between the inoculum density of Verticillium dahliae and the progress of Verticillium wilt of olive. Plant Disease 91, 1372-1378.

LÓpez-Escudero F.J, del Rio RincÓn C, Caballero Reig J.M, Blanco-LÓpez M.A. 2007. Response of olive cultivars to stem puncture inoculation with a defoliating pathotype of Verticillium dahliae. HortScience 42, 294-298.

Martos-Moreno C, LÓpez -Escudero F.J, Blanco-LÓpez M.A. 2006. Resistance of Olive Cultivars to the Defoliating Pathotype of Verticillium dahliae. HortScience 41, 1313-1316.

Owen C.A, Bita E.C, Banilas G, Hajjar S.E, Sellianakis V, Aksoy U. 2005. AFLP reveals structural details of genetic diversity within cultivated olive germplasm from the Eastern Mediterranean. Theoretical and Applied Genetics 110, 1169-1176.

Porras-Soriano A, Soriano-Martin M.L, Porras-Piedra A. 2003. Grafting olive cv. Cornicabra on rootstocks tolerant to Verticillium dahliae reduces their susceptibility. Crop Protection 22, 369-374.

Rodríguez E, García-Garrido J.M, García P.A, Campos M. 2008. Agricultural factors affecting Verticillium wilt in olive orchards in Spain. European Journal of Plant Pathology 122, 287-295.

Rodriguez-Jurado D.R, Blánco-López M.A, Rapoport H.F, Jiménez-Díaz R.M. 1993. Present status of Verticillium wilt of olive in Andalucia (southern Spain). EPPO Bulletin23, 513-516.

Sadras V.O, Quiroz F, Echarte L, Escande A, Pereyra V.R. 2000. Effect of Verticillium dahliae on photosynthesis, leaf expansion and senescence of field-grown sunflower. Annals of Botany 86, 1007-1015.

Sanei S.J, Okhovvat S.M, Hedjaroude Gh.A, Saremi H, Javan-Nikkhah M. 2004. Olive verticillium wilt or dieback on olive in Iran. Commun Applied and Biological Sciences, Ghent University 69, 433-442.

Sanei S.J, Okhovvat S.M, Javan-Nikkhah M, Saremi H. 2005a. Vegetative compatibility and pathogenicity of Verticillium dahliae from olive in Iran. Communications in Agricultural and Applied Biological Sciences 70, 323-326.

Sanei S.J, Okhovvat S.M, Hedjaroud Gh.A, Javan-Nikkhah M, Saremi H. 2005b. Recognition of wild olive resistance to some diseases for breeding and horticultural programs. 57th International Symposium on Crop Protection,Ghent University. p. 194.

Sanei S.J, Waliyar F, Razavi S.E, Okhovvat S.M. 2008. Vegetative compatibility, host range and pathogenicity of Verticillium dahliae isolates in Iran. International Journal of Plant Production2, 37-45.

Sanei S.J, Razavi S.E, Okhovvat S.M, Pahlavani M.H. 2010. Verticillium Wilts. Peik-e-Reihan publication, Gorgan.

Sanei S.J, Razavi S.E. 2011. Reaction of some olive cultivars to Verticillium dahliae isolates agent of vascular wilt: A comparative study. American Journal of Experimental Agriculture 1, 320-330.

Sanei S.J, Razavi S.E. 2017. Sources of Verticillium wilt resistance in wild olive germplasmfrom the Golestan province, Northern Iran. Journal of Crop Protection 6, 457-470.

Saremi H, Amarlou A, Okhovvat S.M. 2006. Verticillium wilt of olive branches in Iran and its control managements with soil solarization method. Communications in Agricultural and Applied Biological Sciences 71, 1187-1196.

Tjamos E.C. 1993. Prospects and strategies in controlling Verticillium wilt of olive. EPPO Bulletin 23(3), 505-512.

Trapero C, Serrano N, Arquero O, Trapero A, LÓpez-Escudero F.J. 2013. Field Resistance to Verticillium Wilt in Selected Olive Cultivars Grown in Two Naturally Infested Soils. Plant Disease 97, 668-674.

Tsror L, Hazanovsky M, Mordechi-Lebiush S, Sivan S. 2001. Agressivness of Verticillium dahliae isolates from different vegetative compatibility groups to potato. Plant Pathology 50, 477- 482.

Wilhelm S. 1955. Longevity of the verticillium wilt fungus in the laboratory and field. Phytopathology45, 180-181.

Wilhelm S, Taylor J.B. 1965. Control of Verticillium wilt of olive through natural recovery and resistance. Phytopathology 55, 310-316.