Impact of Salicylic Acid on Phenolic Metabolism and Antioxidant Activity in Four Grape Cultivars during Cold Storage

Document Type: Research paper

Author

Department of Biology, Payame Noor University (PNU), Iran

Abstract

Salicylic acid (SA) plays an important role in the regulation of plant ripening and responses to abiotic stresses. In this study, the protective effect of SA on cold stress-caused oxidative damage in grape (Vitis vinifera L.) bunches was investigated during cold storage.  Grape bunches treated with 2 mM SA and  stored at 0°C with 85-90% RH for 30 days. Samples were selected from each treatment for physical and chemical analysis before storage, midpoint of storage, end of storage and end of shelf life. The results showed that the cold storage induced lipid peroxidation, decay incidence and loss of fresh weight, while SA pretreatment mitigated these effects in “Bidaneh Sefid” and “Qzl uzum” cultivars. In these cultivars, SA-induced protection against cold damage was accompanied by decrease in malondialdehyde (MDA) content, and caused an increase in catalase (CAT) and superoxide dismutase (SOD) activity. Additionally, SA-induced protection against cold injury was mediated through promoting total phenolics concentration and phenylalanine ammonia-lyase (PAL) activity. The present findings suggest that although variability in the protective effect of SA against cold damage was observed between grape cultivars, SA could alleviate the cold-induced oxidative stress in grape bunches during cold storage.

Keywords


Alleweldt G, Spiegel-Roy P, Reisch B. 1991. Grapes (vitis). Genetic resources of temperate fruit and nut crops. Acta Horticulturae 1, 291-330.

Asghari M, Aghdam M.S. 2010. Impact of salicylic acid on post-harvest physiology of horticultural crops. Trends in Food Science and Technology 21, 502-509.

Babalar M, Asghari M, Talaei A, Khosroshahi A. 2007. Effect of pre-and postharvest salicylic acid treatment on ethylene production, fungal decay and overall quality of Selva strawberry fruit. Food chemistry 105(2), 449-453.

Bonada M, Sadras V.O. 2015. Critical appraisal of methods to investigate the effect of temperature on grapevine berry composition. Australian Journal of Grape and Wine Research 21(1), 1-7.

Cai H, He M, Ma K, Huang Y, Wang Y. 2015. Salicylic acid alleviates cold-induced photosynthesis inhibition and oxidative stress in Jasminum sambac. Turkish Journal of Biology 39(2), 241-247.

Cao S, Hu Z, Zheng Y, Lu B. 2010. Synergistic effect of heat treatment and salicylic acid on alleviating internal browning in cold-stored peach fruit. Postharvest Biology and Technology 58(2), 93-97.

Cao S.F, Hu Z.C, Wang H.O. 2009. Effect of salicylic acid on the activities of antioxidant enzymes and phenylalanine ammonia-lyase in cucumber fruit in relation to chilling injury. The Journal of Horticultural Science and Biotechnology 84(2), 125-130.

Creissen G.P, Mullineaux P.M. 2002. The molecular biology of the ascorbate-glutathione cycle in higher plants. Oxidative stress in plants, Taylor & Francis, UK. 247-270.

Ding C.K, Wang C, Gross K.C, Smith D.L. 2002. Jasmonate and salicylate induce the expression of pathogenesis-related-protein genes and increase resistance to chilling injury in tomato fruit. Planta 214(6), 895-901.

Habibi G. 2014. Hydrogen peroxide (H2O2) generation, scavenging and signaling in plants. In: Ahmad, P. (ed.). Oxidative Damage to Plants: Antioxidant Networks and Signaling. Elsevier, USA. 557-574

Habibi G. 2015. Effect of postharvest silicon application on phenolic content and antioxidant activity of apricot (Prunus armeniaca) fruits. International Journal of Postharvest Technology and Innovation 5(1), 42-51.

Habibi G, Hajiboland R. 2012. Comparison of photosynthesis and antioxidative protection in Sedum album and Sedum stoloniferum (Crassulaceae) under water stress.Photosynthetica50, 508-518

Hayat Q, Hayat S, Irfan M, Ahmad A. 2010. Effect of exogenous salicylic acid under changing environment: a review. Environmental and Experimental Botany 68(1), 14-25.

Heim K.E, Tagliaferro A.R, Bobilya D.J. 2002. Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships. The Journal of nutritional biochemistry 13(10), 572-584.

Kang G.Z, Wang Z.X, Sun G.C. 2003. Participation of H2O2 in enhancement of cold chilling by salicylic acid in banana seedlings. Acta Botanica Sinica 45(5), 567-573.

Karlidag H, Yildirim E, Turan M. 2009. Exogenous applications of salicylic acid affect quality and yield of strawberry grown under antifrost heated greenhouse conditions. Journal of Plant Nutrition and Soil Science 172(2), 270-276.

Knörzer O.C, Lederer B, Durner J, Böger P. 1999. Antioxidative defense activation in soybean cells. Physiologia Plantarum 107(3), 294-302.

Lachowicz S, Oszmiański J, Pluta S. 2017. The composition of bioactive compounds and antioxidant activity of Saskatoon berry (Amelanchier alnifolia Nutt.) genotypes grown in central Poland. Food Chemistry 235, 234-243.

Lee S.K, Mbwambo Z.H, Chung H, Luyengi L, Gamez E.J, Mehta R.G, Kinghorn A.D, Pezzuto J.M. 1998. Evaluation of the antioxidant potential of natural products. Combinatorial Chemistry and High Throughput Screening 1, 35-46.

Luo Z, Wu X, Xie Y. Chen C. 2012. Alleviation of chilling injury and browning of postharvest bamboo shootby salicylic acid treatment. Food Chemistry 131, 456-461.

Lu X.H, Sun D.Q, Mo Y.W, Xi J.G, Sun G.M. 2010. Effects of post-harvest salicylic acid treatment on fruit quality and anti-oxidant metabolism in pineapple during cold storage. The Journal of Horticultural Science and Biotechnology 85(5), 454-458.

Meng X, Li B. Liu J. Tian S. 2008. Physiological responses and quality attributes of table grape fruit to chitosan preharvest spray and postharvest coating during storage. Food Chemistry 106, 501-508.

Panda S.K, Chaudhury I, Khan M.H. 2003. Heavy metals induce lipid peroxidation and affect antioxidants in wheat leaves. Biologia Plantarum 46(2), 289-294.

Posmyk M.M, Bailly C, Szafrańska K, Janas K.M, Corbineau F. 2005. Antioxidant enzymes and isoflavonoids in chilled soybean (Glycine max L. Merr.) seedlings. Journal of Plant Physiology. 162(4), 403-412.

Promyou S, Ketsa S, van Doorn W.G. 2012. Salicylic acid alleviates chilling injury in anthurium (Anthurium andraeanum L.) flowers. Postharvest Biology and Technology 64(1), 104-110.

Ranjbaran E, Sarikhani H, Wakana A, Bakhshi D. 2011. Effect of salicylic acid on storage life and postharvest quality of grape (Vitis vinifera L. cv. Bidaneh Sefid). Journal of the Faculty of Agriculture, Kyushu University 56(2), 263-269.

Lei T, Feng H, Sun X, Dai Q.L, Zhang F, Liang H.G, Lin H.H. 2010. The alternative pathway in cucumber seedlings under low temperature stress was enhanced by salicylic acid. Plant Growth Regulation 60(1), 35.

Tian S, Qin G, Li B, Wang Q. Meng X. 2007. Effects of salicylic acid on disease resistance and postharvest decay control of fruits. Stewart Postharvest Review 6, 1–7

Velikova V, Yordanov I. Edreva A. 2000. Oxidative stress and some antioxidant systems in Acid rain-treated bean plants-protective role of exogenous polyamines. Plant Science 151, 59-66.

Velioglu Y.S, Mazza G, Gao L, Oomah B.D. 1998. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. Journal of Agricultural and Food Chemistry 46(10), 4113-4117.

Wang D, Amornsiripanitch N, Dong X. 2006. A genomic approach to identify regulatory nodes in the transcriptional network of systemic acquired resistance in plants. PLoS Pathogens 2(11), 123.

Yao H, Tian S. 2005. Effects of pre-and post-harvest application of salicylic acid or methyl jasmonate on inducing disease resistance of sweet cherry fruit in storage. Postharvest Biology and Technology 35(3), 253-262.

Yang J, Martinson T.E, Liu RH. 2009. Phytochemical profiles and antioxidant activities of wine grapes. Food Chemistry 116(1), 332-339.

Zoffoli J.P, Latorre B.A, Naranjo P. 2009. Preharvest applications of growth regulators and their effect on postharvest quality of table grapes during cold storage. Postharvest Biology and Technology 51(2), 183-192.

Zucker M. 1965. Induction of phenylalanine deaminase by light its relation to chlorogenic acid synthesis in potato tuber tissue. Physiologia Plantarum40, 779-784.