Document Type : Research paper


1 Assistant Professor, Department of Horticultural Science, Faculty of Agriculture, University of Jiroft, Jirot, Iran.

2 Associate Professor, Department of Horticultural Science, Faculty of Agriculture, University of Guilan, Rasht, Iran.


The pomegranate fruit is a good source of bioactive compounds. The present study has investigated the biochemical and sensory characteristics of the arils of four Iranian commercial pomegranate cultivars namely Malase Shirine Saveh (MSS), Malase Torshe Saveh (MTS), Alak Shirine Saveh (ASS) and Agha Mohammad Ali (AMA) for several developmental and ripening stages during 45–180 days after fruit set (DAFS). The results showed that the total soluble solids (TSS), individual and total anthocyanin concentrations, and color parameters including chroma and a* values significantly increased, in contrast, the total phenolic concentration and color parameters (L* and hue angle) gradually decreased during developmental stages. Six anthocyanin pigments were found responsible for the red color of pomegranate juice. The quantity and the quality of the anthocyanin pigments were different among the cultivars and the various developmental stages. The predominant anthocyanin pigment at all developmental stages in all  cultivars was cyanidin 3,5-diglucosides. The maximum total phenolic concentration was recorded at 45 DAFS for all cultivars. The highest antioxidant activity was recorded at 45 DAFS, and gradually decreased until 135 DAFS.  Similar decrease was observed in total phenolic and flavonoid concentrations. Notably, increasing of antioxidant activity at the late-developmental stage was due to  induction of the flavonoids and anthocyanins accumulation.


Alighourchi H, Barzegar M, Abbasi S. 2008. Anthocyanins characterization of 15 Iranian pomegranate (Punica granatum L.) varieties and their variation after cold storage and pasteurization. European Food Research and Technology 227, 881–887.

Al-Maiman S.A, Ahmad D. 2002. Changes in physical and chemical properties during pomegranate (Punica granatum L.) fruit maturation. Food Chemistry 76, 437-441.

Anonymous. 2015. Statistical book of agricultural of Iran. Iranian Statistical Centre, Tehran, Iran.

Bashir H.A, Abu-Gouck AA. 2003. Compositional changes during guava fruit ripening. Food Chemistry 80, 557-563.

Borochov-Neori H, Judeinstein S, Tripler E, Harari M, Greenberg A, Shomer I, Holland D. 2009. Seasonal and cultivar variations in antioxidant and sensory quality of pomegranate (Punica granatum L.) fruit. Journal of Food Composition and Analysis 22, 189–195.

Brand-Williams W, Cuvelier M.E, Berset C. 1995. Use of a free radical method to evaluate antioxidant activity. LWT - Food Science and Technology 28, 25–30.

Cam M, Yasar H, Gökhan D. 2009. Classification of eight pomegranate juices based on antioxidant capacity measured by four methods. Food Chemistry 112, 721–726.

Candir E.E, Ozdemir A.E, Kaplankiran M, Toplu C. 2009. Physico-chemical changes during growth of persimmon fruits in the East Mediterranean climate region. Scientia Horticulturae 121, 42–48.

D’Aquino S, Palma A, Schirra M, Continella A, Tribulato E, La-Malfa S. 2010. Influence of film wrapping and fludioxonil application on quality of pomegranate fruit. Postharvest Biology and Technology 55, 121–128.

Fawole O.A, Opara U.L. 2013. Developmental changes in maturity indices of pomegranate fruit: A descriptive review. Scientia Horticulturae 159, 152–161.

Gil M.I, Garcia-Viguera C, Artés F, Tomás-Barberán F. 1995. Changes in pomegranate juice pigmentation during ripening. Journal of Agricultural and Food Chemistry 68, 77–81.

Gil M.I, Tomas-Barberan F.A, Hess-Pierce B, Holcroft D.M, Kader A.A. 2000. Anioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. Journal of Agricultural and Food Chemistry 48, 4581–4589.

Gould K, Davies K, Winefield C. 2009. Anthocyanins: Biosynthesis, functions and Applications. Springer Science, Business Media, LLC.

Hernandez F, Melgarejo P, Tomas-Barberran F.A, Artes F. 1999. Evolution of juice anthocyanins during ripening of new selected pomegranate (Punica granatum) clones. European Food Research and Technology 210, 39–42.

Holland D, Hatib K, Bar-Yaakov I. 2009. Pomegranate: botany, horticulture, breeding. Horticultural Reviews 35, 127–191.

Kalaycıoglu Z. Erim F.B. 2017. Total phenolic contents, antioxidant activities, and bioactive ingredients of juices from pomegranate cultivars worldwide. Food Chemistry 221, 496–507.

Kulkarni A.P, Aradhya S.M. 2005. Chemical changes and antioxidant activity in pomegranate arils during fruit development. Food Chemistry 93, 319-324.

Miguel G, Fontes C, Antunes D, Neves A, Martins D. 2004. Anthocyanin concentration of ‘Assaria’ pomegranate fruits during different cold storage conditions. Journal of Biomedicine and Biotechnology 5, 338–342.

Moing A, Svanella L, Rolin D, Gaudillère M, Gaudillère J.P, Monet R. 1998. Compositional changes during the fruit development of two peach cultivars differing in juice acidity. Journal of the American Society for Horticultural Science 123, 770–775.

Moing A, Renaud C, Gaudillère M, Raymond P, Roudeillac P, Denoyes-Rothan B. 2001. Biochemical changes during fruit development of four strawberry cultivars. Journal of the American Society for Horticultural Science 126, 394–403.

Mousavinejad G, Emam-Djomeh Z, Rezaei K, Haddad-Khodaparast M.H. 2009. Identification and quantification of phenolic compounds and their effects on antioxidant activity in pomegranate juices of eight Iranian cultivars. Food Chemistry 115, 1274–1278.

Nuncio-Jáuregui N, Calín-Sánchez A, Carbonell-Barrachina A, Hernández F. 2014. Changes in quality parameters, proline, antioxidant activity and color of pomegranate (Punica granatum L.) as affected by fruit position within tree, cultivar and ripening stage. Scientia Horticulturae 165, 181–189.

Park Y.S, Jung S.T, Kang S.G, Heo B.G, Arancibia-Avila P, Toledo F, Drzewiecki J, Namiesnik J, Gorinstein S. 2008. Antioxidants and proteins in ethylene-treated kiwifruits. Food Chemistry 107, 640-648.

Shwartz E, Glazer I, Bar-Yaakov I, Matityahu I, Bar-Ilan I, Holland D, Amir R. 2009. Changes in chemical constituents during the maturation and ripening of two commercially important pomegranate accessions. Food Chemistry 115, 965–973.

Singleton V.L, Orthofer R, Lamuela-Raventós R.S. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteau reagent. Methods Enzymology 299, 152-178.

Tehranifar A, Zarei M, Nemati Z, Esfandiyari B, Vazifeshenas M.R. 2010. Investigation of physico-chemical properties and antioxidant activity of twenty Iranian pomegranate (Punica granatum L.) cultivars. Scientia Horticulturae 126, 180–185.

Zarei M, Azizi M, Bashir-Sadr Z. 2011. Evaluation of physicochemical characteristics of pomegranate (Punica granatum L.) fruit during ripening. Fruits 66, 121-129.

Zheng H.Z, Kim Y.I, Chung S.K. 2012. A profile of physicochemical and antioxidant changes during fruit growth for the utilisation of unripe apples. Food Chemistry 131, 106–110.