Effect of Salicylic Acid Application on Oxidative Damage and Antioxidant Activity of Grape (Vitis vinifera L.) under Drought Stress Condition

Document Type : Research paper

Authors

1 Associate professor, Department of Biology, Faculty of Sciences, Urmia University, Urmia, West Azarbaijan, Iran.

2 MSc. Student, Department of Horticultural Sciences, Faculty of Agriculture, Urmia University, Urmia.

Abstract

Adaptation and defense responses are the consequences of exposure to drought stress in plants. Salicylic acid (SA) as a natural signaling molecule poses a significant impacts on various aspects of plant growth and development. This study investigates the effects of SA on carotenoids content, lipid peroxidation rate and antioxidant enzymes activities. A pot experiment was conducted using a factorial pattern based on a randomized complete block design with three replications under greenhouse conditions. The variables in the experiment included grapevine cultivars (‘Rasheh’ and ‘Bidane Sefid’), irrigation periods (5, 10 and 15 day intervals) and SA concentrations (0, 1 and 2 mM). Results showed that with increasing the drought stress levels, increase in carotenoids, malondialdehyde (MDA) contents, catalase (CAT), ascorbate peroxidase (APX) and guaiacol peroxidase (GPX) enzymes activities were detected. Carotenoids content, catalase (CAT) and ascorbate peroxidase (APX) and guaiacol peroxidase (GPX) enzymes activities were higher in the Rasheh cultivar compared with the ‘Bidane Sefid’ cultivar. Carotenoids content, CAT, APX and GPX enzymes activities increased by application of salicylic acid. . According to the obtained results, the Rasha cultivar showed a greater tolerance to water stress in comparison with Bidane Sefid cultivar, suggesting that SA is capable of   inducing drought stress  tolerance in plants.

Keywords

References

Abbate P.E, Dardanellib J.L, Cantareroc M, Maturanoc M, Melchiorid R.J.M, Sueroa E.E. 2004. Climatic and water availability effects on water-use efficiency in wheat. Journal of Crop Science 44, 474-483.
Abdulah A, Ghamdi A. 2009. Evaluation of oxidative stress in two wheat (Triticum aestivum L.) cultivars in response to drought. Int. Journal of Agricultural and Biological Engineering 11, 7-12.
Aebi H. 1984. Catalase in vitro. Method Enzymol 105, 121-126.
Agamy R.A, Hafez E.E, Taha T.H. 2013. Acquired resistant motivated by SAapplications on salt stressed tomato (Lycopersicon esculentum Mill). American- Eurasian Journal of Agricultural & Environmental Sciences 13(1), 50-57.
Al-Hakimi A.M.A, Hamada A.M. 2001. Counteraction of salinity stress on wheat plants by grain soaking in ascorbic acid, thiamin or sodium salicylate. Journal of Plant Biology 44, 253-261.
Arora A, Singh V.P, Mohan J. 2001. Effect of nitrogen and water stress on photosynthesis and nitrogen content in wheat. Biologia Plantarum 44(1), 153-155
Asada K. 1992. Ascorbate peroxidase and hydrogen peroxide scavenging enzyme in plants. Journal of Plant Physiology 30, 987-998.
Asada K, Takahashi M. 1987. Production and scavenging of active oxygen in photosynthesis. In: Kyle DJ Osmond CB Arntzen CJ (eds.) Photo-inhibition topics in photosynthesis.  Elsevier Amsterdam 9, 227-287.
Ashraf M.Y, Mazhar H, Naqvi L, Khan A.H. 1996. Effect of water stress on total phenols, peroxidase activity, Growth and yield on tomato. Acta Horticulturae 516, 41-45.
Azooz M.M, Ismail A.M, Elhamd M.F. 2009. Growth, lipid peroxidation and antioxidant enzyme activities as a selection criterion for salt tolerance of maize cultivars grown under salinity stress. International Journal of Agricultural and Biological Engineering 11, 21-26.
Bian S, Jiang Y. 2009. Reactive oxygen species, antioxidant enzyme activities and gene expression patterns in leaves and roots of Kentucky bluegrass in response to drought stress and recovery. Scientia Horticulturae 120, 264-270.
Bradford M.M. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Annual Review of Biochemistry 72, 248-254.
Chae H.S, Faureand F, Kieber J.J. 2003. The eto1, eto 2, and eto 3 mutations and cytokinin treatment increase ethylene biosynthesis in Arabidopsis by increasing the stability of ACS Protein. The Plant Cell 15, 545-559.
Chen Z, Iyer S, Caplan A, Klessig D.F, Fan B. 1997. Differential accumulation of SAand salicylic acidsensitive catalase in different rice tissue. Journal of Plant Physiology 114, 193-201.
Cunhuva S, Wei D, Xiangling C, Xinna X, Yahong Z, Dong S, Jianjie S. 2010. The Effects of drought stress on the activity of acid phosphatase and its protective enzymes in pigweed leaves. African Journal of Biotechnology 9, 825-833.
Cornelia P, Petrus A, Pop L, Chis A, Bandici G.E. 2010. Exogenous SAinvolvement on some physiological parameters amelioration in salt stressed wheat (Triticum aestivum) plantlets. Analele Universitatii din Oradea, Fascicula: Protectia Mediului 15, 160- 165.
Cramer G.R, Lauchli A, Epstein E. 1986. Effect of NaCl and CaCl2 on ion activites in complex nutrient solution and root growth of cotton. Journal of Plant Physiology 81, 792-797.
Decov I, Tsonev T, Yordanov I. 2000. Effects of water stress and high-temperature stress on the structure and activity of photosynthetic apparatus of Zea mays and Helianthus annuus. Photosynthetica 38, 361-366.
Dellongo O.T, Gonzales G.A, Pastori G.M. 1993. Antioxidant defenses sensitivity to drought. Plant and Cell Physiology 34(7), 1023-1028.
Doulatabadian A, Modarressanavy S.A.M, Etemadi F. 1386. Effect of pretreatment of SAon wheat (Triticum aestivum L.) seed germination under salt stress. Iranian Journal of Basic Medical Sciences 4, 15-21.
Efeoglu B, Ekmekci Y, Çicek N. 2009. Physiological responses of three maize cultivars to drought stress and recovery. South African Journal of Botany 75(1), 34-42.
Elizabeth M.A, Boach S. 2008. SAmay be involved in the regulation of drought- induced leaf senescence in perennials. Environmental and Experimental Botany 64, 105-112.
El_Tayeb M.A. 2005. Response of barley grains to the interactive effect of salinity and salicylic acid. Plant Growth Regulation 45, 215-224.
Fariduddin Q, Hayat S, Ahmad A. 2003. SAinfluences net photosynthetic rate, carboxilation efficiency, nitrate reductase activity and seed yield in Brassica juncea L. Photosynthetica 41, 281-284.
Farooq M, Wahid A, Kobayashi N, Fujita D, Basra S.M.A. 2009. Plant drought stress: effects, mechanisms and management. Agronomy for Sustainable Development 29, 185-212.
Filella I, Llusia J, Pin J.O, Pen J.U. 1998. Leaf gas exchange and fluorescence of Phillyrea latifolia, Pistacia lentiscus and Quercus ilex saplings in severe drought and high temperature conditions. Environmental and Experimental Botany 39, 213-220.
Gharib F.A. 2006. Effect of SAon the growth, metabolic activities and oil content of basil and marjoram. International Journal of Agricultural and Biological Engineering 4, 485-492.
Gill S.S, Tuteja N. 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry 48, 909-930.
Hajheidari M, Abdollahian- Noghabi M, Askari H. 2005. Proteome analysis of sugar beet leaves under draught stress. Proteomics 5, 463-99.
Hasheminasab H, Assad M.T, Aliakbari A, Sahhafi R. 2012. Influence of drought stress on oxidative damage and antioxidant defense systems in tolerant and susceptible wheat genotypes. Journal of Agricultural Science 4(8), 20-30.
Hayat S, Ahmad A. 2007. Salicylic acid: A plant hormone. Springer Publ, Dordrecht.
Hayat S, Hasan S.A, Fariduddin Q, Ahmad A. 2008. Growth of tomato (Lycopersicon sculentum L.) in response to SAunder water stress. Journal of Plant Interactions 3, 297-304.
Hayat Q, Hayat S, Irfan M, Ahmad A. 2010. Effect of exogenous SAunder changing environment: A review. Environmental and Experimental Botany 68, 14-25.
Heath R.L, Packer L. 1968. Photoperoxidation in isolated chloroplast. I. Kinetics and stoichiometry of fatty acid peroxidation. Biochemistry and Biophysics 125, 189-198.
Horvath E, Pal M, Szalai G. 2007. Exogenous 4-hydroxy benzoic acid and SAmodulate the effect of short-term drought and freezing stress on wheat plants. Biologia Plantarum 51, 480-487
Horvath E, Szalai G, Janda T. 2007. Induction of abiotic stress tolerance by SAsignaling. Journal of Plant Growth Regulation 26, 290-300.
Hussain M, Malik M.A, Farooq M, Ashraf M.Y, Cheema M.A. 2008. Improving Drought tolerance by exogenous application of glycine betaine and SAin sunflower. Journal of Agronomy and Crop Science 194, 193-199.
Janda T, Horvat E, Szalai G, Paldi E. 2007. Role of SAin the induction of abiotic stress tolerance. Biologia Plantarum 51, 471-477.
Josaph B, Jini D, Sujatha S. 2010. Insight into role of exogenous SAon plants growth under salt environment. Asian Journal of Crop Science 2(4), 226-235.
Juan M, Rivero R.M, Romero L, Ruiz J.M. 2005. Evalu­ation of some nutritional and biochemical indicators in selecting salt-resistant tomato cultivars. Environmental and Experimental Botany 54, 193-201.
Kadioglu A, Saruhan N, Saglam A, Terzi R, Acet T. 2011. Exogenous SAalleviates effects of long term drought stress and delays leaf rolling by inducing antioxidant system. Plant Growth Regulation 64, 27-37.
Kavar T, Maras M, Kidric M, Sustar-Vozlic J, Meglic V. 2007. Identification of genes involved in the response of leaves of Phaseolus vulgaris to drought stress. Molecular Breeding 21, 159-172.
Kaya M.D, Okçub G, Ataka M, Çkılıc Y, Kolsarıcıa O. 2006. Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). The European Journal of Agronomy 24, 291-295.
Khan W, Balakrishnan P, Smith D.L. 2003. Photosynthetic responses of corn and soybean to foliar application of salicylates. Journal of Plant Physiology 160(5), 485-492.
Khan M.I.R, Syeed S, Nazarand R, Anjum N.A. 2012. An insight into the role of SAand jasmonic acid in salt stress tolerance. In: Phytohormones and abiotic stress tolerance in plants. Springer 277-300.
Khodary S.E.A. 2004. Effect of SA on growth, photosynthesis and carbohydrate metabolism in salt stressed maize plants. International Journal of Agricultural and Biological Engineering 6, 5-8.
King H.M, Saltveit M.E. 2002. Chilling tolerance of maize, cucumber and rice seedling leaves and roots are differentially affected by salicylic acid. Journal of Plant Physiology 115, 571-576.
Kumar P, Dube S.D, Chauhan V.S. 1999. Effect of SAon growth, development and some biochemical aspect of soybean (Glycine max L. Merrill). International Journal of Plant Physiology and Biochemistry 4, 327-330.
Lazaridou M, Kirilov A, Noitsakis B, Todorov N, Katerov I. 2003. The effect of water deficit on yield and water use efficiency of lucerne. Optimal forage systems for animal production and the environment, Proceedings of the 12th Symposium of the European Grassland Federation, Pleven, Bulgaria.
Lazaridou M, Koutroubas S.D. 2004. Drought effect on water use efficiency of berseem clover at various growth stages. New directions for a diverse planet: Proceedings of the 4th International Crop Science Congress Brisbane, Australia. 26 Sept–1 Oct 2004.
Lichtenthaler H.K, Wellburn A.R. 1983. Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochemical Society Transactions 11, 591-592.
Manivannan P, Abdul Jaleel C, Somasundaram R, Panneersel R. 2008. Osmoregulation and antioxidant metabolism in drought-stressed Helianthus annus L. under triadimefon drenching.  The Comptes rendus Biologies 331, 418–425.
Meirs S, Philisohhadas S, Aharoni N. 1992. Ethylene increased accumulation of florescent lipid- per oxidation products detected during parsley by a newly developed method. Journal of the American Society for Mass Spectrometry 117, 128-132.
Mohammadi A, Habibi D, Rihami M, Mafakheri S. 2011. Effect of Drought Stress on Antioxidant Enzymes Activity of Some Chickpea Cultivars American-Eurasian L. American-Eurasian journal of agricultural & environmental sciences 11(6), 782-785.
Mohammadkhani N, Heidari R. 2008. Effects of drought stress on soluble proteins in two maize varieties. The Turkish Journal of Biology 32, 23-30.
Moharekar S.T, Lokhande S.D, Hara T, Tanaka R, Tanaka A, Chavan P.D. 2003. Effect of SAon chlorophyll and carotenoid contents of wheat and moongcaryopsis. Photosynthetica 41, 315-317.
Moussa H, Abdel-Aziz S.M. 2008. Comparative Response of Drought Tolerant and Drought Sensitive Maize Genotypes to Water Stress. Australian Journal of Crop Science 1, 31-36.
Nazar R, Iqbal N, Syeed S, Khan N.A. 2011. SAalleviates decreases in photosynthesis under salt stress by enhancing nitrogen and sulfur assimilation and antioxidant metabolism differentially in two mungbean cultivars. Journal of Plant Physiology 168, 807-815.
Nguyen H.T, Babu R.C, Blum A. 1997. Breeding for drought resistance in rice: Physilogy and molecular genetics considerations. Crop Science 37, 1426-1434.
Qin J, Wang X, Hu F, Li H. 2010. Growth and physiological performance responses to drought stress undernon-flooded rice cultivation with straw mulching. Plant, Soil and Environment 56(2), 51-59.
Rubio M.C, Minchin F.R. 2002. Effect of water stress on antioxidant enzyme of leaves and nodules of transgenic alfalfa over-expressing superoxide dismutase. Journal of Plant Physiology 115, 531-540.
Sagee O, Riov J, Goren R. 1989. Ethylene-enhanced catabolism of (14C) indole-3- acetic acid to indole-3-carboxylic acid in citrus leaf tissues. Journal of Plant Physiology l91, 54-60.
Sairam R.K, Deshmukh P.S, Saxena D.C. 1998. Role of antioxidant systems in wheat genotype tolerance to water stress. Biologia Plantarum 41(3), 387-394b.
Sarkar S, Topha C. 1974. Effects of ethylene on the phenylalanine ammonia-lyase activity of carrot tissues.  Journal of Plant Physiology 32(4), 318.
Sedghi M, Sharifi R.S, Pirzad A.R, Balaneji B.A. 2012. Phytohormonal regulation of antioxidant systems in petals of drought stressed pot marigold (Calendula officinalis L.). Journal of Agricultural Science and Technology 14, 869-878.
Senaratna T, Touchell D, Bunn T, Dixon K. 2000. Acetyl SA(Aspirin) and SAinduce multiple stress tolerance in bean and tomato plants. Plant Growth Regulation 30, 157-161.
Shao H.B, Chu L.Y, Wu G, Zhang J.H, Lu Z.H, Hu Y.C .2007. Changes of some anti-oxidative physiological indices under soil water deficits among 10 wheat (Triticum aestivum L.) genotypes at tillering stage. Journal of Colloid and Interface Science 54, 143-149.
Shakirova F.M, Sakhabutdinova A.R, Bezrukova M.V, Fathudinova R.A, Fathutdinova D.R. 2003.  Insight into the role of exogenous salicylic acid on plants grown under salt environment. Plant Science 164, 317.
Sharma P, Dubey R.S. 2005. Drought induces oxidative stress and enhances the activities of antioxidant enzymes in growing rice seedlings. Plant Growth Regulation 46, 209-221.
Sing B, Usha K. 2003. SAinduced physiological and biochemical changes in wheat seedlings under water stress. Plant Growth Regulation 39, 137-141.
Smart D.R, Carlise E, Goebel M, Nunez A. 2005. Transverse hydraulic redistribution by a grapevine.Plant, Cell and Environment 28, 157-166.
Smirnof N. 1993. The role of active oxygen in the response of plants to water deficit and desiccation. The New Phytologist Trust 125, 27-58.
Syeed S, Anjum N.A, Nazar R, Iqbal N, Masood A, Khan N.A. 2011. Salicylic acid-mediated changes in photosynthesis, nutrients content and antioxidant metabolism in two mustard (Brassica juncea L.) cultivars differing in salt tolerance. Acta Physiologiae Plantarum 33, 877-886.
Szepesi A, Csiszar J, Bajkan S, Gemes K, Horvath F, Erdel L, Deer A.K, Simon M.L, Tari L. 2005. Role of SApre-treatment on the acclimation of tomato plants to salt and osmotic stress. Acta Biologica Szegediensis 49, 123- 125.
Uupdhyaya A, Sankhla D, Davis T.D, Sankhla N, Smidth B.N. 1985. Effect of paclobutrazol on the activities of some enzymes of activated oxygen metabolism and lipid peroxidation in senescing soybean leaves. Journal of Plant Physiology 121, 453-461.
Vallivodan B, Nguyen H.T. 2006. Understanding regulatory networks and engineering for enhanced drought tolerance in plants. Current Opinion in Plant Biology 9, 189-195.
Wang Y.S, Wang J, Yang Z.M, Wang Q.Y, Lu B, Li S.Q, Lu Y.P, Wang S.H, Sun X. 2004. SAmodulates aluminum-induced oxidative stress in roots of Cassia tora L. Acta Botanica Sinica 46, 819-828.
Wang W.B, Kim Y.H, Lee H.S, Kim K.Y, Deng X.P, Skwak S. 2009. Analysis of antioxidant enzyme activity during germination of alfalfa under salt and drought stress. Plant Physiology and Biochemistry 47, 570-577.
Wise M.J, Tunnacliffe A. 2004. POPP the question: what do LEA proteins do? Trends in Plant Sciences 9, 7-13.
Zhou Zh.Sh, Guo K, Abdou Elbaz A, Yang Zh M. 2009. SAalleviates mercury toxicity by preventing oxidative stress in roots of Medicago sativa. Environmental and Experimental Botany 65, 27-34.
International Journal of Horticultural Science and Technology
Volume 4, Issue 1
June 2017
Pages 29-50

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History

  • Receive Date: 10 February 2016
  • Revise Date: 09 October 2017
  • Accept Date: 13 April 2017

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