Genetic Variation within Iranian Iris Species Using Morphological Traits

Azimi, Mohammad Hossein; Tahernezhad, Zahra; Zamani, Mohammad Javad

doi:10.22059/ijhst.2016.58165

Document Type : Research paper

Authors

¹ Assistant Professor, National Institute of Ornamental Plants (NIOP), Horticulture Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Mahallat, Iran

² Seed and Plant Certification and Registration Institute, Karaj, Iran

³ Seed and Plant Certification and Registration Institute, Karaj, Iran.

https://doi.org/10.22059/ijhst.2016.58165

Abstract

Iris belongs toIridaceae family and it is monocotyledon. Iris is one of the important ornamental and medicinal plants. 34 iris genotypes (14 species) collected from different provinces of Iran were planted at National Institute of Ornamental Plants (NIOP) Iran. All of the species evaluated for 15 quantitative traits and 30 qualitative traits. Results showed that the highest positive correlation was between inner tepal length and width and the lowest of this correlation was between crest length and width. Cluster analysis using Ward similarity coefficient divided Iris species into three clusters. Also the highest Nei’s genetic distance based on qualitative traits was between I. iberica and I. fosterana and the lowest was between I. germanica and I. paradoxa. The maximum genetic diversity was in Khorasan Razavi populations, and the minimum was in Khorasan (North). According to Shannon index, I. spuria and I. germanica species had the maximum level of genetic diversity. Cluster analysis according to qualitative traits by Jaccard similarity coefficient and UPGMA classified the 34 genotypes into four groups. Classification of populations based on quantitative traits was not similar to classification based on qualitative traits and they were not similar to geographical distribution.

Keywords

References

Arafe, R.M.H. Sapir, Y. Shmida, A. Iraki, N. Fragman, and H. O.Comes. 2002. Pattern of genetic and phenotypic variation in Iris haynei, I.atrofusca (Iris sect. Oncocyclus = the royal Irises) along an ecogeographical gradient Israel and the West Bank. Mol. Ecol. 11:39-53.

Avishai, M. 1977. Species relationships and cytogenetic affinities in section Oncocyclus of the genus Iris. Unpublished PhD Thesis, The Hebrew University of Jerusalem.

Avishai, M. and Zohary, D. 1980. Genetic affinities among the Oncocyclus Irises. Bot. Gaz.141:107–115.

Azimi, M.H. Hamzehi, Z. Khalag, M.A. and Fathihafashjani, A. 2012. Evaluation of genetic variety and heritability of some characteristics of wildspecies Iris of Iran. Int. J Agron. Plant Prod. 3(11):514-520.

Azimi, M.H. Sadeghian, S.Y. Ahari, V. Khazaei and A. F. Fathi-Hafashjani. 2012. Genetic variation of Iranian Iris species using morphological characteristics and RAPD markers. Int. J. Agr. Sci. 2(9): 875-889.

Brauner, S. D.J. Crawford and T.F. Stuessy. 1992. Ribosomal DNA and RAPD variation in the rare plant family Lactoridaceae. Amer. J. Bot. 79:1436-1439.

Feinbrun-Dothan, N. 1986. Flora Palaestina. Jerusalem: Israel. Academy of Sciences and Humanities.

Ghahraman, A. 1998. Colorful Flora of Iran. The Research Institute of Forest and Pastures, Tehran (in Farsi).

Ghahreman, A. 1979. Colorful Flora of Iran. The Research Institute of Forest and Pastures, Tehran (in Farsi).

Hamrick, J.L. and M.J.W. Godt. 1990. Allozyme diversity in plant species. In: Plant Population Genetics, Breeding, and Genetic Resources (eds Brown AHD, Clegg MT, Kahler AL, Weir BS), pp: 43-63.

Karam, N.S. A.A. Al-Khasawneh, R.A. Ghibli, H.M. Migdadi and M. Al-Ajlouni. 2004. Morphological variation in Black Iris (Iris nigricans Dinsm.) from Jordan. FAO- Biodivers. 147:12-17.

Khassanov, F.O. and N. Rakhimova. 2012. Taxonomic revision of the genus Iris L. (Iridaceae Juss.) for the flora of Central Asia. Stapfia 97:174–179.

Low, A.J. O. Hanotte and L. Guarino. 1996. Standardization of molecular genetics techniques for the characterization of germplasm collections: The case for Random Amplified Polymorphic DNA (RAPD). Plant Gen. Res. Newslett. 107:50- 54.

Mathew, B. 1989. The iris London: Batsford. 23-76.

Moradi, B. 2007. Evaluation of genetic diversity of quantitative characters and superior single plants selection for propagation in Gladiolus different varieties. Final Report. Publication of Research Station of Ornamental Plant Center at Mahalat. Iran. P: 65.

Naderi, R. M. Alaei and A. Khalighi. 2007 Classification of cyclamen races in Iran using evaluation of important morphological traits. 5th Horticulture Science Congress. Shiraz. Iran. P: 106.

Nei, M. 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89:583–590.

Peakall, R. and P.E. Smouse. 2006. GENALEX6: genetic analysis in Excel. Population genetic software for teaching and research. Mol. Ecol. Notes 6:288- 295.

Rahimi, V. M. Arab, S.H. Dianati and R. Amiri. 2009. Evaluation of morphological diversity of local Irises Iran. 6th Iranian Holticulture Science Congress. P: 324.

Rix, M. 1997. Section Oncocyclus(Siemssen) Baker. In The Species Group of the British Iris Society, A guide to species of Irises. Cambridge University Press. Cambridge 62– 90.

Sapir, Y. A. Shmida, O. Fragman and H.P. Comes. 2002. Morphological variation of the OncocyclusIrises(Iris: Iridaceae) in the southern Levant. Bot. J. Linnean Soc. 139:369–382.

Wendelbo, P. 1977. Tulips and Irises of Iran. Botanical Institute of Iran, Tehran, Iran.

International Journal of Horticultural Science and Technology

Genetic Variation within Iranian Iris Species Using Morphological Traits

References

References

Volume 3, Issue 1
June 2016
Pages 89-98

Genetic Variation within Iranian Iris Species Using Morphological Traits

References

References

Volume 3, Issue 1June 2016Pages 89-98

Volume 3, Issue 1
June 2016
Pages 89-98