A Simple and Efficient Direct Shoot Organogenesis Method Using Leafy Petiole Explants in Gerbera jamesonii ‘Royal Soft Pink’

Document Type : Research paper

Authors

1 Department of Horticultural Science, College of Agriculture, University of Kurdistan, Sanandaj, Iran

2 Department of Horticultural Science, College of Agriculture, Shiraz University, Shiraz, Iran

3 Agriculture Biotechnology Research Institute of Iran, Karaj, Iran

4 Ornamental Plants Research Institute of Iran, Mahallat, Iran

Abstract

The gerbera market would benefit from an efficient and simple protocol for high rate regeneration for propagation and genetic engineering. With these objectives, this investigation was done on shoot regeneration via direct organogenesis from leafy petiole explants of Gerbera jamesonii ‘Royal Soft Pink’. Murashige and Skoog (1962) (MS) medium was supplemented with 0.1 mg L-1 indole-3-acetic acid (IAA) and additions of various concentrations and combinations of thidiazuron (TDZ: 0, 0.5 and 1 mg L-1) and N6-benzyladenine (BA: 0, 2, 4 and 6 mg L-1). Higher values were recorded for a number of shoots on leafy petiole on the MS medium containing BA in combination with TDZ than on media containing BA or TDZ solely. The highest evaluations for percentage of shoot regeneration (85.43 %) and number of shoots per explant (12.88) was recorded in the medium supplemented with 0.1 mg L-1 IAA and 1.0 mg L-1 TDZ plus 4.0 mg L-1 BA. For rooting of the shoots, MS medium supplemented with three concentrations of a-naphthaleneacetic acid (NAA: 0.5 and 1 mg L-1) together with control (MS only) were tried. The optimal results for rooting of shoots were obtained on MS medium containing 1 mg L-1 NAA. The in vitro raised plantlets were acclimatized and transferred to greenhouse successfully.
 

Keywords

References

Akter, N., M.I. Hoque, and R.H. Sarker. 2012. In vitro Propagation in Three Varieties of Gerbera (Gerbera jamesonii Bolus.) from Flower Bud and Flower Stalk Explants. Plant Tissue Cult. Biotech. 22:143-152.
Ananthakrishnan, G., R. Ravikumar, R. Prem Anand, G. Vengadesan, and A. Ganapathi. 1999. Induction of Somatic Embryogenesis from Nucellus Derived Callus of Anacardium occidentale L. Scientia Hort. 79:91-99.
Bhatia, R., K.P. Singh, T. Jhang, and T.R. Sharma. 2009. Assessment of Clonal Fidelity of Micropropagated Gerbera Plants by ISSR Markers. Scientia. Hort. 119:208-211.
Cardoso, J. C. and J. A. Teixeira da Silva. 2012. Micropropagation of Gerbera Using Chlorine Dioxide (ClO2) to Sterilize the Culture Medium. In Vitro Cell. Dev. Biol. Plant. 48:362–368.
Cardoso, J. C. and J. A. Teixeira da Silva. 2013. Gerbera Micropropagation. Biotechnol. Adv. 31:1344-1357.
Chen, F.C., C.Y. Wang, and J.Y. Fang. 2012. Micropropagation of Self-Heading Philodendron via Direct Shoot Regeneration. Scientia Hort. 141:23–29.
Dole, J.M. and H.F. Wilkins. 2005. Floriculture Principles and Species. Prentice- Hall, Inc. USA, 1023 pp.
Elomaa, P., J. Honkanen, R. Puska, P. Seppiinen, Y. Helariutta, M. Mehto, M. Kotilainen, L. Nevalainen, and T.H. Terri. 1993. Agrobacterium-Mediated Transfer of Antisense Chalcone Synthase cDNA to Gerbera hybrida Inhibits Flower Pigmentation. Biotechcol. 11:508-511.
Gantait, S., N. Mandal, S. Bhattacharya, and P.K. Das. 2010. An Elite Protocol for Accelerated Quality-Cloning in Gerbera jamesonii Bolus cv. Sciella. In Vitro Cell. Dev. Biol. Plant 46:537-548.
Goldy, R.G. 1991. Growth Regulator and Axillary Bud Position Effects on In Vitro Establishment of Vitis rotundifolia. HortScience 26:304-307.
Guo, B., B.H. Abbasi, A. Zeb, L.L. Xu, and Y.H. Wei. 2011. Thidiazuron: a Multidimensional Plant Growth Regulator. Afr. J. Biotechnol. 10:8984-9000.
Hasbullah, N.A., A. Saled, and R.M. Taha. 2011. Establishment of Somatic Embryogenesis from Gerbera jamesonii Bolus ex. Hook F. Through Suspension Culture. Afr. J. Biotechnol. 10:13762–13768.
Hedtrich, C.M., 1979. Sprossregeneration Aus Blatern Und Vermehrung Von Gerbera jamesonii. Gartenbauwissenschaft 44:1-3.
Huetteman, C.A. and J.E. Preece. 1993. Thidiazuron: a Potent Cytokinin for Woody Plant Tissue Culture. Plant Cell Tissue Organ Cult. 33:105-119.
Husain, M.K., M. Anis, and A. Shahzad. 2007. In Vitro Propagation of Indian Kino (Pterocarpus marsupium Roxb.) Using Thidiazuron. In Vitro Cell. Dev. Biol. Plant 43:59–64.
Jerzy, M. and M. Lubomski. 1991. Adventitious Shoot Formation on Ex Vitro Derived Leaf Explants of Gerbera jamesonii. Scientia Hort. 47:115–124.
Koné, M., T. Koné, H.T. Kouakou, S. Konaté, and J.S. Ochatt. 2013. Plant regeneration Via Direct Shoot Organogenesis from Cotyledon Explants of Bambara groundnut (Vigna subterranea L.). Biotechnol. Agron. Soc. Environ. 17:584-592.
Lata, H., S. Chandra, I. Khan, and M.A. ElSohly. 2009. Thidiazuron-Induced High-Frequency Direct Shoot Organogenesis of Cannabis sativa L. In Vitro Cell. Dev. Biol. Plant 45:12-19.
Liu, C.Z., S.J. Murch, E.L. Demerdash, and P.K. Saxena. 2003. Regeneration of the Egyptian Medicinal Plant Artemisia judaica L. Plant Cell Rep. 21:525-530.
Lu, C. 1993. The Use of Thidiazuron in Tissue Culture. In Vitro Cell. Dev. Biol. Plant. 29:92–96.
Malik, K.A. and P.K. Saxena. 1992. Thidiazuron Induces High Frequency Shoot Regeneration in Intact Seedling of Pea (Pisum sativum) Chickpea (Cicer arietinum) and Lentil (Lens culinaris).  Austral. J. plant physiol. 19:731–740.
McConnell, J.R. and M.K. Barton. 1998. Leaf Polarity and Meristem Formation in Arabidopsis. Dev. 125:2935-2942.
Nazari, F., M. Khosh-Khui, P. Azadi, H. Salehi, and A. Niazi. 2014. Growth regulators Affected In Vitro Propagation of Pot Gerbera (Gerbera jamesonii cv. Royal Soft Pink). Intl. J. Agr. Biosci. 3:185-189.
Nielsen, J. M., J. Hansen, and K. Brand. 1995. Synergism of Thidiazuron and Benzyladenine in Axillary Shoot Formation Depends on Sequence of Application in Miscanthus × ogiformis cv. Giganteus. Plant Cell Tissue Organ Cult. 41: 165–170.
Orlikowska, T., E. Nowak, A. Marasek, and D. Kucharska. 1999. Effects of Growth Regulators and Incubation Period on In Vitro Regeneration of Adventitious Shoots from Gerbera Petioles. Plant Cell Tissue Organ Cult. 59:95–102.
Polisetty, R., V. Paul, J.J. Deveshwar, S. Khetarpal, K. Suresh, and R. Chandra. 1997. Multiple Shoot Induction by Benzyladenine and Complete Plant Regeneration from Seed Explants of Chickpea (Cicer arietinum. L.). Plant Cell Rep. 16:565–571.
Sreekumar, S., S. Mukunthakumar, and S. Seeni. 2001. Morphogenetic Responses of Six Philodendron Cultivars In Vitro. Indian J. Exp. Biol. 39:280–1287.
Sudarsono, A., and R.G. Goldy. 1991. Growth Regulator and Axillary Bud Position Effects on In Vitro Establishment of Vitis rotundifolia. HortScience. 26:304-307.
Teeri, T.H., P. Elomaa, M. Kotilainen, and V.A. Albert. 2006. Mining Plant Diversity: Gerbera as a Model System for Plant Developmental and Biosynthetic Research. Bioassays 28:756–767.
Thiruvengadam, M., K.T. Rekha, C.H. Yang, N. Jayabalan, and I.M. Chung. 2010. High Frequency Shoot Regeneration From Leaf Explants Through Organogenesis in Bitter Melon (Momordica charantia L.). Plant Biotechnol. Rep 4:321–328.
Thomas, T.D. and J.T. Puthur. 2004. Thidiazuron Induced High Frequency Shoot Organogenesis in Callus From Kigelia pinnata L. Bot. Bul. Acad. Sinica 45:307–313.
Tran Thanh Van, M. 1973. In Vitro Control of De Now Flower, Bud, Root and Callus Differentiation From Excised Epidermal Tissues. Nature 246:44-45.
International Journal of Horticultural Science and Technology
Volume 3, Issue 1
June 2016
Pages 51-58

Files

History

  • Receive Date: 10 September 2015
  • Revise Date: 09 March 2016
  • Accept Date: 01 May 2016

Share

How to cite

Statistics