Paclobutrazol-Induced Biochemical Changes in Pomegranate (Punica granatum L.) cv. ‘Malas Saveh’ under Freezing Stress

Document Type: Research paper

Authors

1 Department of Horticultural Sciences, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran

2 Pomegranate Research Station. Saveh Agriculture Research Center. Saveh. Iran.

3 Professor and Director of Pomology and Viticulture Program, University of Idaho, Parma Research and Extension Center, 29603 U of I Lane, Parma, ID 83660, USA

Abstract

Freezing injury is an important limiting factor in the production of pomegranate in Iran.The aim of the present study was to evaluate the effect of paclobutrazol (PBZ) on cold hardiness of pomegranate (Punica granatum L.) cv. ‘Malas Saveh’. Different concentrations of PBZ including 0 (control), 31, 62, 125 or 250 mg L–1 were sprayed on one-year-old plants in August and the electrolyte leakage of their stems was measured at three acclimation stages (November, January, and March). PBZ treatments, especially at 125 and 250 mg L–1 concentrations increased cold hardiness, and corresponding soluble carbohydrates and proline contents. The highest variation in freezing tolerance was observed between control and PBZ-treated plants in January, and the lowest found in November. Irrespective of PBZ treatment, correlations between cold hardiness and soluble carbohydrate concentrations were stronger, compared to proline. Soluble carbohydrates were higher in January, associated with deep dormancy, whereas the maximum proline content was detected in March, at deacclimation stage. Results suggest that PBZ application can reduce low temperature-induced dysfunction of cell membrane through increasing soluble carbohydrates and proline contents.

Keywords


[1].Ahmedullah, M., A. Kawahami, C.R. Sandidge., and R.L. Wample. 1986. Effect of Paclobutrazol on the Vegetative Growth, Yield, Quality, and Winter Hardiness of Buds of Concord Grape. HortScience 21:273-274.
[2].Ali Khan, L. 1998. Effects of Growth Regulators on Cold Hardiness of Pecan (Carya illinoinensis) (Wangen.) at Various Temperatures. Univ. of Mississippi, Mississippi, PhD Diss.
[3].Améglio, T., M. Decourteix, G. Alves, V. Valentin, S. Sakr, J.L. Julien, G. Petel, A. Guilliot., and A. Lacointe. 2004. Temperature Effects on Xylem Sap Osmolarity in Walnut Trees: Evidence for a Vitalistic Model of Winter Embolism Repair. Tree Physiol. 24:785-793.
[4].Anonymous. 2012. Iran statistical yearbook. Area Under Cultivation and Production of Selected Perennial Crops. Statistical Center of Iran Press.
[5].Arzani, K., F, Bahadori., and S, Piri. 2009. Paclobutrazol Reduces Vegetative Growth and
Enhances Flowering and Fruiting of Mature ‘J.H. Hale’ and ‘Red Skin’ Peach Trees. Hort. Environ. Biotechnol. 50:84-93.
[6].Arzani, K., and H.R. Roosta. 2004. Effects of Paclobutrazol on Vegetative and Reproductive Growth and Leaf Mineral Content of Mature Apricot (Prunus armeniaca L.) Trees. J. Agr. Sci. Technol. 6:43-56.
[7].Arzani, K., and N. Yazdani. 2008. The Influence of Drought Stress and Paclobutrazol on Quantitative Changes of Proteins in Olive (Olea europaea L.) Cultivars `Bladi' and `Mission'. Acta Hort. 791:527-530.
[8].Baninasab, B. 2009. Amelioration of Chilling Stress by Paclobutrazol in Watermelon Seedlings. Sci. Hort. 121:144-148.
[9].Bates, L.S., R.P. Waldren., and I.D. Teare. 1973. Rapid Determination of Free Proline for Water Stress Studies. Plant Soil. 39: 205-207.
[10]. Chen, W.P., and P.H. Li. 2002. Membrane Stabilization by Abscisic Acid under Cold Aids Proline in Alleviating Chilling Injury in Maize 

(Zea mays L.) Cultured Cells. Plant Cell Environ. 25:955-962.
[11]. Coleman, W.K., and E.N. Estabrooks. 1992. Enhancement of Cold Hardiness in Apple Tree by PBZ, Thidiazuron and Flurprimidol. Can. J. Plant Sci. 72:1267-1274.
[12]. Coleman, W.K., E.N. Estabrooks, M. O’Hara, J. Embleton., and R.R. King. 1992. Seasonal Changes in Cold Hardiness, Sucrose and Sorbitol in Apple Trees Treated with Plant Growth Regulators. HortScience 67:429-435.
[13]. Davis, T.D., G.L. Steffens., and N. Sankhla. 1988. Triazole Plant Growth Regulators. Hort. Rev. 10: 63-105.
[14]. Delaunay, A.J., and D.P.S. Verma. 1993. Proline Biosynthesis and Osmoregulation in Plants. Plant J. 4: 215-223.
[15]. Feng, Z., A. Guo., and Z. Feng. 2003. Amelioration of Chilling Stress by Triadimefon in Cucumber Seedlings. Plant Growth Regul. 39:277-283.
[16]. Fiorino, P., and S. Mancuso. 2000. Differential Thermal Analysis, Supercooling and Cell Viability in Organs of Olea europaea at Subzero Temperatures. Adv. Hort. Sci. 14:23-27.
[17]. Fletcher, R.A., A. Gilley, N. Sankhla, and T.D. Davis. 2000. Triazoles as Plant Growth Regulators and Stress Protectants. Hort. Rev. 24:55-137.
[18]. Ghasemi Soluklui, A.A., A. Ershadi., and E. Fallahi. 2012. Evaluation of Cold Hardiness in Seven Iranian Commercial Pomegranate (Punica granatum L.) Cultivars. Hortscience 47:1821-1825.
[19]. Gopi, R., C. Abdul Jaleel, R. Sairam, G.M.A. Lakshmanan, M. Gomathinayagam, and R. Panneerselvam. 2007. Differential Effects of Hexaconazole and Paclobutrazol on Biomass, Electrolyte Leakage, Lipid Peroxidation and Antioxidant Potential of Daucus carota L. Colloids and Surfaces B: Biointerfaces 60:180-186.
[20]. Kraus, T.E., and R.A. Fletcher. 1994. PBZ Protects Wheat Seedlings from Heat and Paraquat Injury. Is Detoxification of Active Oxygen Involved? Plant Cell Physiol. 35:45-52.
[21]. Lin, K.H., F.H. Pai, S.Y. Hwang., and H.F. Lo. 2006. Pre-treating with Paclobutrazol Enhanced Chilling Tolerance of Sweet Potato. Plant Growth Regul. 49:249-262.
[22]. Maage, F. 1986. The Effect of Growth Regulators on Bud Dormancy and Winter Injury in Red Raspberry. Acta Hort. 179:149–156.
[23]. Mauk, K.C., C.R. Unrath, S.M. Blankenship., and L.J. Lehman. 1990. Influence of Method of Application of Paclobutrazol on Soil Residues and Growth Retardation in A 'Starkrimson-Delicious' Apple Orchard. Plant Growth Regul. 9:27-35.
[24]. Morin, X., T. Ameglio, R. Ahas, C. Kurz-Besson, V. Lanta, F. Lebourgeois, F. Miglietta., and I. Chuine. 2007. Variation in Cold Hardiness and Carbohydrate Concentration from Dormancy Induction to Bud Burst Among Provenances of Three European Oak Species. Tree Physiol. 27:817-825.
[25]. Pagter, M., C.R. Jensen, K.K. Petersen, F. Liu., and R. Arora. 2008. Changes in Carbohydrates, ABA and Bark Proteins During Seasonal Cold Acclimation and Deacclimation in Hydrangea Species Differing in Cold Hardiness. Physiol. Plant. 134:473-485.
[26]. Palonen, P. 1999. Relationship of Seasonal Changes in Carbohydrates and Cold Hardiness in Canes and Buds of Three Red Raspberry Cultivars. J. Amer. Soc. Hort. Sci. 124:509-513.
[27]. Palonen, P., and D. Buszard. 1997. Current State of Cold Hardiness Research on Fruit Crops. Can. J. Plant Sci. 77:399-420.
[28]. Reynolds A.G., D.A. Wardle, A.C. Cottrell., and A.P. Gaunce. 1992. Advancement of ‘Riesling’ Fruit Maturity by Paclobutrazol-induced Reduction of Lateral Shoot Growth. J. Amer. Soc. Hort. Sci. 117:430-435.
[29]. Santarius, K.A. 1992. Freezing of Isolated Thylakoid Membranes in Complex Media. VIII. Differential Cryoprotection by Sucrose, Proline and Glycerol. Physiol. Plant. 84:87-93.
[30]. Sarkhosh, A., Z. Zamani, R. Fatahi., and H. Ranjbar. 2009. Evaluation of Genetic Diversity Among Iranian Soft-Seed Pomegranate Accessions by Fruit Characteristics and RAPD Markers. Scientia Hort. 121:313-319.
[31]. SAS Institute. 2003. SAS version 9.1. SAS Institute, Cary, North Carolina, USA.
[32]. Senaratna, T., C. Mackay, B. McKersie., and R. Fletcher. 1988. Uniconazole-induced Chilling Tolerance in Tomato and its Relationship to Antioxidant Content. Plant Physiol. 133:56-61.
[33]. Shao, H.B., B. Li, B.C. Wang, K.E. Tang., and Y.I. Liang. 2008. A Study on Differentially Expressed Gene Screening of Chrysanthemum 

Plants under Sound Stress. CR. Biol. 331:329-333.
[34]. Srivastav, M., A. Kishor, D. Anil., and R.R. Sharma. 2010. Effect of Paclobutrazol and Salinity on Ion Leakage, Proline Content and Activities of Antioxidant Enzymes in Mango (Mangifera indica L.). Scientia Hort. 125:785-788.
[35]. Srivastav, M.S., R.R. Sharma., and R.N. Khawale. 2003. Residual Effect of Paclobutrazol on Yield and Fruit Quality of Mango Cultivars. Indian J. Plant Physiol. 483-488 (special issue).
[36]. Steffens, G.L., and S.Y. Wang. 1986. Biochemical and Physiological Alterations in Apple Trees Caused by A Gibberellin Biosynthesis Inhibitor PBZ. Acta Hort. 179:433-442.
[37]. Sugavanam, B. 1984. Diastereoisomers and Enantiomers of PBZ: Their Preparation and Biological Activity. J. Pesticide Sci. 15:296-302.
[38]. Tafazoli, E., and C. Beyl. 1993. Changes in Endogenous Abscisic Acid and Cold Hardiness in Actinidia Treated with Triazole Growth Retardants. Plant Growth Regul. 12:79-83.
[39]. Vu, J.C., and G. Yelenosky. 1992. Growth and Photosynthesis of Sweet Orange Plants Treated with Paclobutrazol. Plant Growth Regul. 11:85-89.
[40]. Walser, R.H., and T.D. Davis. 1986. Cold Hardiness, Rest, and Growth of Peach Trees as Influences by Paclobutrazol. Proc. Plant Growth Reg. Soc. Amer. 13:177-181.
[41]. Wang, S.Y., G.L. Steffens., and M. Faust. 1986. Effect of Paclobutrazol on Acclimation of Carbohydrates in Apple Wood. HortScience 22:276-278.
[42]. Wanner, L.A., and O. Junttila. 1999. Cold-induced Freezing Tolerance in Arabidopsis. Plant Physiol. 120:391-399.
[43]. Yelenosky, G., J.C. Vu., and H.K. Wutscher. 1995. Influence of Paclobutrazol in the Soil on Growth, Nutrient Elements in the Leaves, and Flood/Freeze Tolerance of Citrus Rootstock Seedlings. Plant Growth Regul. 14:129-134.
[44]. Yemm, E.W., and A.J. Willis. 1954. The Estimation of Carbohydrates in Plant Extracts by Anthrone. Biochem. J. 57:508-514.