Document Type : Research paper
Authors
1
Dr. Mousa RasouliAssociate Professor of Horticultural Sciences (Physiology and Breeding of fruit trees), Department of Horticultural Sciences Engineering, Faculty of Agriculture and Natural Resources, Imam Khomeini International University,
2
MSc. Graduate, Department of Horticultural Science, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran
3
Department of Horticultural Science, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran
4
MSc. Graduate of Horticulture Science and Landscape Department, Faculty of Agriculture, Malayer University, Malayer, Hamedan, Iran
5
Departament of Plant Breeding CEBAS-CSIC, PO Box 164, E-30100 Murcia, Spain
Abstract
In this study, pollen compatibility as well as identification of self-incompatibility alleles of selected cultivars and genotypes including ‘MSK81’, ‘MSK82’, ‘MSK83’, ‘MSK84’, ‘Shahrood12’ cultivar and ‘Shokufeh’ as paternal cultivar with ‘Shahrood12’ cultivar as parent with two controlled crossbreeding methods in orchard conditions and the polymerase chain reaction was investigated. The results of controlled pollination under garden conditions showed that the highest percentage of fruit set (14.39%) was obtained at the crossing of ‘Shahrood12’ and ‘Shokufeh’ cultivars. The lowest percentage of fruit set (0%) was obtained in pollination of ‘Shahrood12’ cultivar with self-pollen, which showed complete self-incompatibility of this cultivar. The degenerate primers used in this study identified 8 incompatibility alleles and an unknown SA allele with a band size of 550 bp in the studied cultivars and genotypes. The combination of CEBASf and AmyC5R primers did not identify any bands indicating self-compatibility in the genotypes and cultivars studied. The highest amount of kernel oil was obtained as a result of the crossing of ‘Shahrood12’ maternal parent with ‘MSK82’ paternal parent at 54.42%. Also, in the study of the amount of fatty acids, it was found that in the offspring of the cross, the highest amount of oleic acid was obtained in the offspring of the cross between the parent ‘Shahrood12’ and the parent ‘Shokufeh’ (72%). The highest amount of linoleic acid was obtained from the crossing of ‘Shahrood12’ parent with ‘MSK84’ father (18.40%). Also, the highest percentage of palmitic acid (9.50%) was observed in the results of free pollination.
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