Document Type : Research paper
Authors
1
Phd student, Department of Agricultural Sciences and Food Industries, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2
Associate Professor of Department of Agricultural Sciences and Food Industries, Science and Research Branch, Islamic Azad University, Tehran, Iran Weed science
3
Assistant Prof., Seed and Plant CertificationAssociate Professor- Seed and Plant Certification and Registration Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran.
4
Teacher,University of Science and Re, Assistant Professor of Department of Agricultural Sciences and Food Industries, Science and Research Branch, Islamic Azad University, Tehran, Iransearch
5
Associate Professor, Department of Agricultural Sciences and Plant Breeding, University of Tehran, Tehran, Iran
Abstract
The use of saline water for the production of halophyte forage is a reasonable practice in production of fodder where there is a shortage of fresh water reserves. The aim of this research was to investigate the effect of removing seed hardness and salt stress on germination characteristics and the activity of antioxidant enzymes in the seeds of Alhagi camelorum. A factorial experiment was carried out in the form of a completely random design with four repetitions. The factors examined included the Iranian population (Gorgan and Mashhad), the salinity stress (control, 100 and 200 mM NaCl) and seed hardness removal (water at 100 ℃ for 30 seconds and concentrated sulfuric acid for 30 minutes). Results showed that salinity stress had a very significant effect on the percentage and rate of germination, seedling length and seedling dry weight. The seeds of two populations of Alhagi camelorum were able to tolerate and germinate in saline conditions up to 200 mM salinity, although the germination indices decreased with increasing NaCl concentration. The level of CAT increased by 77.9% and 75.1% in Mashhad and Gorgan populations, at 200 mM salinity and 100°C water treatment compared to the control, respectively. The seed dormancy breaking treatment with 100 ℃ water at 200 mM salinity stress, increased SOD activities in Mashhad and Gorgan populations by 56.9% and 45.4% compared to the control, respectively. The salinity tolerance of Mashhad population of Alhagi camelorum seeds treated with 30-minute immersion in sulfuric acid showed 56.9% increase compared to the control.
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