Khaled Elmeer; Zenib Suliman; Latifa Al-Latifi; Nora Alhashimi; Ateea Alamami
Abstract
In the Libyan Green Mountains, this study was conducted to evaluate the effects of different elevations and topography on the genetic variation and chemical composition of Spartium junceum. The results indicated a significant difference among the antioxidant activity, phenolic compounds, and flavonoid ...
Read More
In the Libyan Green Mountains, this study was conducted to evaluate the effects of different elevations and topography on the genetic variation and chemical composition of Spartium junceum. The results indicated a significant difference among the antioxidant activity, phenolic compounds, and flavonoid content of flower extracts obtained from the genotypes of different regions. The total phenol content ranged from 22.74 to 31.66 mg GAE g-1, whereas the flavonoid content ranged from 7.35 to 10.74 mg CE g-1 DW. The antioxidant efficiency appeared variable among the flower extracts, ranging between 66.81-91.55 μM Trolox equivalents (TE) g-1 of dry matter. Genetic variation was examined by inter-simple sequence repeats, showing a mean value of 0.26 in gene diversity, and a mean value of 0.21 in polymorphism information content. A total of 26 bands were observed, with an average of 3.25 bands per primer, while 50% of the bands were polymorphic. The most effective primers were BT01, BT09, and BT11, which generated four bands. The least effective primer was BT10, which generated only two bands. Phylogenetic relationships between the genotypes divided the samples into two main groups according to geographical location.
Mehdi Garazhian; Ali Gharaghani; Saeid Eshghi; Ahmad Tahmasebi
Abstract
This study was carried out to estimate the genetic diversity and relationships of 74 Iranian blackberry accessions assigned to 5 different species using inter-simple sequence repeats (ISSR) marker analysis and morphological trait characterization. Sixteen traits were analyzed, including phenological, ...
Read More
This study was carried out to estimate the genetic diversity and relationships of 74 Iranian blackberry accessions assigned to 5 different species using inter-simple sequence repeats (ISSR) marker analysis and morphological trait characterization. Sixteen traits were analyzed, including phenological, vegetative and reproductive attributes (in 57 out of 74 accessions), and 10 ISSR primers were screened (in 74 accessions). The yield and leaf width had the highest and lowest genetic diversity, (diversity index = 62.57 and 13.74), respectively. Flowering and ripening date were recorded as traits with the strongest correlations (r = 0.98). Ten ISSR primers were selected and produced a total of 161 amplified fragments (200 to 3500 bp), of which 113 were polymorphic. The highest, lowest and average PIC values were 0.53, 0.38 and 0.44, respectively. Principle component analysis (PCA) was based on morphological traits and showed that the first six components explained 84.9% of the variations among the traits. Meanwhile, the principal coordinate analysis (PCoA) was based on ISSR data and implied the first eight principal coordinates which explained 67.06% of the total variation. Cluster analysis was based on morphological traits and ISSR data ultimately classified all accessions into two and three major groups, respectively, so that the distribution pattern of genotypes was mainly based on species and the geographic origins.
Hamed Hassanzadeh Khankahdani; Abdoolnabi Bagheri
Abstract
Date palm (Phoenix dactylifera L.) is a monocot, dioecious and high longevity tropical plant greatly considered due to its high economic importance in Middle East. This investigation was carried out to detect genetic relations among 34 cultivars of Iranian date palm, both males and females, using ISSR ...
Read More
Date palm (Phoenix dactylifera L.) is a monocot, dioecious and high longevity tropical plant greatly considered due to its high economic importance in Middle East. This investigation was carried out to detect genetic relations among 34 cultivars of Iranian date palm, both males and females, using ISSR markers. Accordingly, total DNA was extracted using CTAB method and was subjected to PCR amplification using 12 ISSR primers. Analysis of ISSR data indicated that PIC value varied from 0.3695 to 0.4998 with average of 0.4497. The highest PIC value belonged to (CT)10 G primer (0.4498). Totally, 112 alleles were recognized. According to morphological attributes and ISSR markers, the 34 studied date cultivars divided into eight main groups and different sub-groups, and no significant correlation between grouping made by morphological and molecular markers. Unlike ISSR markers that produced a clear-cut among male and female cultivars, no distinct border was seen among male and female cultivars using morphological attributes. Furthermore, PCA analysis confirmed the grouping made by ISSR markers. In conclusion, ISSR markers have been useful to separate date palm cultivars.
Hamed Hassanzadeh Khankahdani; Somayeh Rastegar; Behrouz Golein; Morteza Golmohammadi; Abdolhossein Aboutalebi Jahromi
Abstract
Because of sexual propagation of Mexican lime in southern regions of Iran, there are many lime accessions (lime biotypes) in Mexican lime gardens in which appear some variation in fruit and tree shape. However, most of these accessions are susceptible to witches broom disease of lime (WBDL). Persian ...
Read More
Because of sexual propagation of Mexican lime in southern regions of Iran, there are many lime accessions (lime biotypes) in Mexican lime gardens in which appear some variation in fruit and tree shape. However, most of these accessions are susceptible to witches broom disease of lime (WBDL). Persian lime (C. latifolia Tanaka) is a triploid WBDL-tolerant species. Considerable number of un-described lime biotypes have been cultivated by farmers as Persian lime with no documented evidence related to their origin and genetic background. To unveil systematic modality of the lime accessions, we investigated 18 lime accessions collected from Fars, Hormozgan and Mazandaran provinces (Iran) using flow cytometry (FCM) approach, morphological characteristics, ISSR and SSR markers in 2016. The results of FCM revealed that 14 out of 18 lime accessions were triploid. Triploid accessions showed higher leaf dimension values together with lower stomata and secretory sacs density compared with diploid accessions. Application of SSR markers confirmed the obtained results of ploidy level determination using FCM and morphological analysis. Results of morphological and ISSR markers demonstrated that four out of 18 accessions were different from others and it was in correspondence with the results of FCM. SSR results also grouped the accessions in line of FCM. In conclusion, it has been shown that IFJKh, Cucumber-shaped lime and IFJKMes accessions, are diploid and their cultivation is not recommended in the infected regions to WBDL phytoplasma.
