Ritambhara Bhutani; Susmita Shukla; Nibha Gupta; Shiv Kant Shukla
Abstract
This research aimed at in vitro propagations of bananas originating from India. Banana (Musa spp.) is a nutritious fruit but shows susceptibility to specific diseases. A traditional method of its propagation is through the separation of suckers, although it may culminate in the transmission of nematodes, ...
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This research aimed at in vitro propagations of bananas originating from India. Banana (Musa spp.) is a nutritious fruit but shows susceptibility to specific diseases. A traditional method of its propagation is through the separation of suckers, although it may culminate in the transmission of nematodes, parasitic organisms, and viral diseases. In the past two decades, plant tissue culture techniques have facilitated the production of disease-free plantlets. Tissue culturing bananas can involve different explants, including shoot tips, suckers, leaves, and flower buds. Each responds differently to the presence and absence of light. In the current research, explant cultures were placed in light or dark incubation for identical durations, i.e., one to six months, to monitor their growth and development. The color intensity of the explants changed in response to different photoperiods. Young flower buds, mature flower buds, and suckers developed a higher color intensity when placed in light than in dark conditions. However, the opposite occurred in leaf explants thatgrew optimally in the dark. The results indicated that banana growth occurred productively from sucker explants in different light conditions and variable durations. The results were optimal when employing sucker explants, which exhibited the fastest growth.
Adel Sultan Alqadasi; Isam Al-madhagi; Abdulbaset Al-kershy; Mohamed Al-Samaei
Abstract
Ginger is unfertile species that usually failed to set seed. Cultivation of this plant using its economic part (rhizomes) is an unprofitable method, which negatively affects its supply in the market. Therefore, this study aimed to maintain the local Yemeni ginger variety, and facilitate its propagation ...
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Ginger is unfertile species that usually failed to set seed. Cultivation of this plant using its economic part (rhizomes) is an unprofitable method, which negatively affects its supply in the market. Therefore, this study aimed to maintain the local Yemeni ginger variety, and facilitate its propagation by tissue culture technique, using fresh rhizome buds in a semi-rigid culture medium. To do so, two experiments were carried out, the first one was based on completely randomized block design with four replicates to determine the optimum pH levels (including 5.6, 5.8, 6.0) for the shoot and root formation. The result showed that the 5.8 is the optimum pH level of the medium. Second experiment was designed as two factors, type of cytokinin [6-benzylaminopurine (BAP) and Kiniten (Kin) (N6- furfuryladenine)] and ii) Cytokinin concentrations (0, 1, 2 and 3 mg L-1). The MS basal medium supplemented with above types and concentration of cytokinin with 1.0 mg L-1 IBA and 3% sucrose (BAP and Kinetin) The explants cultured on Murashige and Skoog’s (MS) medium supplemented with cytokinin with four replicates. The result showed that the explants cultured on MS basal medium supplemented with 2.0 mg L-1 Kin + 1.0 mg L-1 IBA give the highest rate of shoot multiplication, shoot length and root number. In conclusion, the result obtained from this study might help to manage the propagation of ginger.
