Document Type : Research paper
Authors
1
Department of Horticultural Science, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran
2
Department of Horticulture, Faculty of Agriculture and Natural Resources, Mohaghegh Ardabili University, Ardabil, Iran, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
3
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Abstract
Linkage maps are the framework for using genetic markers in biotechnological programs. In this experiment, two widely used markers were applied to find the loci of some momentous ornamental traits in lily flowers. A mapping population of 100 F1 progeny was used to construct the genetic linkage maps using the CP (out breeder full-sib family) model of JoinMap4 software. A total of 940 primers were tested, and the best ones were 172 primer pairs, including 96 AFLP markers and 76 SSR markers used for map construction. A total of 616 loci were scored, with 465 loci for the AFLP marker and 151 for the SSR marker. The whole mapped length was 2144.2 cM. The overall number of mapped loci was 189 loci, of which 152 loci were assessed by AFLP markers and 47 by SSR markers. The full length of linkage groups for the maternal map was 861.6 cM with 12 linkage groups. The shortest and longest linkage group gaps in the maternal maps was 2.2 cM and 55.7 cM, respectively. The number of mapped loci in maternal genetic linkage maps was 52 loci, consisting of 42 for the AFLP markers and 10 for the SSR markers. In paternal maps, the mapped loci were 51, and the linkage group’s entire length was 676 cM. The shortest linkage group gap for the paternal map was 0.1 cM, while the longest gap was 47.7 cM. In integrated plants, 6 linkage groups were found with 86 completely assessed loci. The total length of the linkage group for integrated maps was 606.6 cM. The shortest and longest gap of LGs was 0.2 cM and 26.8 cm, respectively.
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