International Journal of Horticultural Science and Technology

International Journal of Horticultural Science and Technology

Improving Effects of Mild Cold Stress and Salicylic Acid on Growth and Physiology of Periwinkle (Catharanthus roseus Don.)

Document Type : Research paper

Authors
Azizolah Khandan-Mirkohi
Mahdieh Khalili Halbi
Seyed Alireza Salami
Hosein Lesani
Department of Horticulture Sciences, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
10.22059/ijhst.2017.208435.126
Abstract
To study effects of salicylic acid (SA) treatments (0, 0.5, 1 and 2 mM) in two forms of seed priming (for 24 h) and spraying on seedling at 4-6 leaf stage an experiment was conducted on periwinkle (Catharanthus roseus Don.) with and without exposure to a mild cold stress of 8 °C (for 32 h). Seeds were sown in trays containing peat-based substrate (with 20% sandy loam field soil). Transplants of both groups were treated with mild cold. Seedlings were grown in greenhouse with an average day/night temperature of 25/20± 2°C until their blooming. Seed emergence rate and percentage, activities of catalase (CAT), guaiacol peroxidase (GPX), polyphenol oxidase (PPO), CAT gene expression, height and number of nodes per plant, and days to flowering were evaluated. Results showed that mild cold stress together with salicylic acid at 0.5 mM (as either seed priming or spray on seedling) was the best treatment to accelerate the flowering, and improve growth parameters and antioxidant enzymatic activities. Thus, mild cold stress enhanced the positive effects of SA treatments on cold acclimation of periwinkle (particularly when associated with spraying, which is a simple administration method). Accordingly, it might be recommended for its seedling production and cultivation in temperate climates to prevent late spring frost damages.
Keywords
Antioxidant enzymes
Medicinal ornamental plant
Plant growth regulator(s)
Stress
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International Journal of Horticultural Science and Technology
Volume 4, Issue 1
June 2017
Pages 67-78

  • Receive Date 30 May 2016
  • Revise Date 06 August 2017
  • Accept Date 06 May 2017