Influences of Brassinosteroide and Hot Water on Postharvest Enzyme Activity and Lipid Peroxidaion of Lime (Citrus aurantifolia L.) Fruit During Storage at Cold Temperature

Document Type : Research paper

Authors

1 Horticulture PhD Student, Department of Horticultural Science, Faculty Agriculture, University of Tabriz, East Azerbaijan, Iran

2 Assistant Professor, Horticultural Research Institute, Shahid Bahonar University of Kerman, Iran

Abstract

Storage of Lime (Citrus aurantifolia L.) fruits, originally a tropical fruits, in low temperature confronts with several difficulties  due to the risk of chilling injury (CI). To develop an effective method aiming to  reduce CI, the effects of treatments containing 0, 0.5 and 1 Mg/lit brassinosteroids (BRs) and hot water (HW) including 20°C as control, 45 and 55°C for 30 minute on CI was studied. Moreover lipid peroxidaion, hydrogen peroxide, catalase (CAT) and peroxidase (POD) activities were investigated in lime fruits stored at 1°C for 21 days. The CI, lipid peroxidaion, hydrogen peroxide were significantly reduced by BRs treatment particularly at 1Mg/lit and 45°CHW treatment. Furthermore fruits treated with 1Mg/lit BRs and 45 °C HW treatment exhibited significantly higher CAT and POD activities in comparison with the control fruits. These results suggest that BRs and HW treatment protect lime fruits from CI by enhancing antioxidant enzymes such as POD and CAT activities, and reduce lipid peroxidaion, hydrogen peroxide contents, and maintaining membrane integrity.

Keywords

References

Abu-Kpawoh J.C, Xi Y.F, Zhang Y.Z, Jin Y.F. 2002. Polyamine accumulation following hot-water dips influences chilling injury and decay in ‘Friar’ plum fruit. Journal Food Science 67, 2649–2653.
Beers R.F, Sizer I.W. 1952. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. The Journal of Biological Chemistry 195, 133-140.
Bradford M.M. 1976. A rapid and sensitive method for the quantittion of microgram quantities of protein utilizing the principle dye binding. Annual Review of Biochemistry 72, 248-252.
Bajguz A, Hayat S. 2009. Effects of brassinosteroids on the plant responses to environmental stresses. Plant Physiology and Biochemistry 47, 1–8.
Bandurska H. 1993. In vitro and in vivo effect of proline on nitrate reductase activity under osmotic stress in barley. Acta Physiologiae Plantarum 15, 83–88.
Bohnert H.J, Jensen R.G. 1996. Strategies for engineering water-stress tolerance in plants. Trends in Biotechnology 14, 89–97.
Chen J.Y, He L.H, Jiang Y.M, Wang Y, Joyce D.C, Ji Z.L, Lu W.L. 2008. Role of phenylalanine ammonia-lyase in heat pretreatment-induced chilling tolerance in banana fruit. Physiologia Plantarum 132, 318–328.
8.Cao S, Xu Q, Cao Y, Qian K, An K, Zhu Y, Binzeng H, Zhao H, Kuai B. 2005. Loss of function mutations in DET2 gene lead to an enhanced resistance to oxidative stress in Arabidopsis. Physiologia Plantarum 123, 57–66.
Civello P.M, Martinez G.A, Chaves A.R, Anon M.C. 1997. Heat treatment delay ripening and postharvest decay of strawberry fruit. Journal of Agricultural and Food Chemistry 45, 4589-4594.
Cowley J.M, Chad field K.D, Baker R.T. 1992. Evaluation of dry heat as a postharvest disinfestation treatment for persimmons. Journal of Crop and Horticultural Science 20, 209-215.
Candir E.E, Temizyurek F, Ozdemir A.E. 2008. The effects of hot water dip treatments on the cold storage of Big Top nectarines. Journal of Applied Botany and Food Quality 82, 136-140.
Ershova A, Khripach V. 1996. Effect of epibrassinolide on lipid peroxidation in Pisum sativum at normal aeration and under oxygen deficiency. Journal of Plant Physiology 43, 750–752.
Enejoh O.S, Ogunyemi I.O, Bala M.S., Oruene I.S, Suleiman M.M, Ambali S.F. 2015. Ethnomedical Importance of Citrus Aurantifolia (Christm) Swingle. The Pharma Innovation Journal 4(8), 1-6.
Fariduddin Q, Yusuf M, Chalkoo S, Hayat S, Ahmad A. 2011. Homobrassinolide improves growth and photosynthesis in Cucumis sativus L. through an enhanced antioxidant system in the presence of chilling stress. Photosynthetica 49, 55–64.
Grover A, Sinha S.K. 1985. Senescence of detached leaves in pigeon pea and chick pea: Regulation by developing pods. Physiologia Plantarum 65, 503-507
He R.Y, Wang G.J, Wang X.S. 1991. Effects of brassinolide on growth and chill-ing resistance of maize seedlings. Brassinosteroids Chemistry, Bioactivity and Application. In: Cultler G.H, Yokota T, Adam G. American Chemical Society, Washington. 220–230.
Hodges D.M, Delong J.M, Forney C.F, Prange R.K. 1999. Improving the thiobar-bituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta 207, 604–611.
Hotta Y, Tanaka T, Luo B.S, Takeuchi Y, Konnai M. 1998. Improvement of cold resistance in rice seedlings by 5-aminolevulinic acid. Journal of Pest Science 23, 29–33.
Heath R.L, Packer L. 1968. Photoperoxidation in isolated chloroplast I. kinetics and stoichiometry of fatty acids peroxidation. Archives of Biochemistry and Biophysics 125, 189-198.
Kochba J, Lavee S, Spiegel-Roy P. 1977. Differevces in peroxidase activity and isoenzymes in embryogenic and non-embryogenic shamouti orange ovular callus lines. Plant and Cell Physiology 18, 463-497.
Lay-Yee M, Ball S, Forbes S.K, Woolf A.B. 1997. Hot water treatment for insect disinfestation and reduction of chilling sensitivity of ‘Fuyu’ persimmon. Postharvest Biology and Technology 10, 81-88.
Lafuente M.T, Zacarias L, Martinez-Tellez M.A, Sanchez-Ballesta M.T, Granell A. 2003. Phenylalanine ammonia-lyase and ethylene in relation to chilling injury as affected by fruit age in citrus. Postharvest Biology and Technology 29, 308–317.
Lafuente M.T, Sala J.M, Zacarias L. 2004. Active oxygen detoxifying enzymes and phenylalanine ammonia-lyase in the ethylene-induced chilling tolerance in citrus fruit. Journal of Agricultural and Food Chemistry 52, 3606–3611.
Li B.Q, Zhang C.F, Cao B.H, Qin G.Z, Wang W.H, Tian S.P. 2012. Brassinolide enhances cold stress tolerance of fruit by regulating plasma membrane proteins and lipids. Amino Acids 43, 2469-80.
Liu Y.J, Zhao Z.G, Si J, Di C.X, Han J, An L.Z. 2009. Brassinosteroids alleviate chilling-induced oxidative damage by enhancing antioxidant defense system in suspension cultured cells of Chorispora bungeana. Plant Growth Regulation 59, 207–214.
Mandava N.B. 1988. Plant growth-promoting brassinosteroids. Annual review of plant physiology and plant molecular biology 39, 23–52.
Nakashita H, Yasuda M, Nitta T, Asami T, Fujikoa S, Arai Y, Sekimata K, Takat-suto S, Yamaguchi I, Yoshida S. 2003. Brassinosteroids functions in a broad range of disease resistance in tobacco and rice. The Plant Journal 33, 887–898.
Obenland D, Collin S, Sievert J, Fjeld K, Doctor J, Arpaia M.L. 2008. Commercial packing and storage of navel oranges alters aroma volatiles and reduces flavor quality. Postharvest Biology and Technology 47, 159–167.
Ozdemir A.E, Dundar, O. 1999. Effects of hot water dip applications on postharvest of some oranges (in Turkish). National Horticule Congress, Ankara. 126-131.
Ozdemir F, Bor M, Demiral T, Turkan I. 2004. Effects of 24-epibrassinolide on seed germination, seedling growth, lipid peroxidation, proline content and antioxida-tive system of rice (Oryza sativa L.) under salinity stress. Plant Growth Regulation 42, 203–211.
Ogweno J.O, Song X.S, Shi K, Hu W.H, Mao W.H, Zhou Y.H, Yu J.Q, Nogues S. 2008. Brassinosteroids alleviate heat-induced inhibition of photosynthesis by increasing carboxylation efficiency and enhancing antioxidant systems in Lycopersicon esculentum. Plant Growth Regulation27, 49–57.
Porat R, Pavoncello D, Peretz Y, Weiss B, Cohen L, Ben-Yehoshua S, Fallik E, Droby S, Lurie S. 2000. Induction of resistance against Penicillium digitatum and chilling injury in ‘Star Ruby’ grapefruit by a short hot water brushing treatment. The Journal of Horticultural Science and Biotechnology 75, 428- 432.
Rady M.M. 2011. Effect of 24-epibrassinolide on growth, yield, antioxidant system and cadmium content of bean (Phaseolus vulgaris L.) plants under salinity and cadmium stress. Scientia Horticulturae 129, 232–237.
Robinson J.M, Bunce J.A. 2000. Influence of drought-induced water stress on soy-bean and spinach leaf ascorbate-dehydroascorbate level and redox status. International Journal of Plant Sciences 161, 271–279.
Sharp R.E, Hsiao T.C, Silk W.K. 1990. Growth of maize primary root at low water potential’s. Plant Physiology 93, 1337–1346.
Velikova V, Yordanov I, Edreva A. 2000. Oxidative stress and some antioxidant system in acid rain treated bean plants: Protective role of exogenous polyamines. Plant Science 151, 59-66.
Yadegari L.Z, Heidari R, Carapetian J. 2007. The influence of cold acclimation on proline, malondialdehyde (MDA), total protein and pigments contents in soybean (Glycine max) seedlings. The Journal of Biological Sciences 7, 1436–1441.
Yu J.Q, Zhou Y.H, Ye S.F, Huang L.F. 2002. 24-Epibrassinolide and abscisic acid protect cucumber seedlings from chilling injury. The Journal of Horticultural Science and Biotechnology 77, 470–473.
Yusuf M, Fariduddin Q, Ahmad A. 2011. 28-Homobrassinolide mitigates boron induced toxicity through enhanced antioxidant system in Vigna radiata plants. Chemosphere 85, 1574-1584.
Zhu Z, Zhang Z, Qin G, Tian S. 2010. Effects of brassinosteroids on postharvest disease and senescence of jujube fruit in storage. Postharvest Biology and Technology 56, 50–55.
Wang C.Y. 1998. Heat treatment affects postharvest quality of kale and collard but not of brussels sprouts. HortScience  33, 881-883.
Woolf A.B, Ball S, Spooner K.J, Lay-Yee M, Ferguson I.B, Watkins C.B, Gunson A, Forbes S.K. 1997. Reduction of CI in the sweet persimmon ‘Fuyu’ during storage by dry air heat treatments. Postharvest Biology and Technology 11, 155-164.
International Journal of Horticultural Science and Technology
Volume 4, Issue 1
June 2017
Pages 57-65

Files

History

  • Receive Date: 15 May 2016
  • Revise Date: 16 September 2017
  • Accept Date: 14 April 2017

Share

How to cite

Statistics